{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T10:20:41Z","timestamp":1790072441355,"version":"4.0.1"},"reference-count":53,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2022,6,14]],"date-time":"2022-06-14T00:00:00Z","timestamp":1655164800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002261","name":"Russian Foundation for Basic Research","doi-asserted-by":"publisher","award":["20-04-60302"],"award-info":[{"award-number":["20-04-60302"]}],"id":[{"id":"10.13039\/501100002261","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>The virucidal activity of a series of cationic surfactants differing in the length and number of hydrophobic tails (at the same hydrophilic head) and the structure of the hydrophilic head (at the same length of the hydrophobic n-alkyl tail) was compared. It was shown that an increase in the length and number of hydrophobic tails, as well as the presence of a benzene ring in the surfactant molecule, enhance the virucidal activity of the surfactant against SARS-CoV-2. This may be due to the more pronounced ability of such surfactants to penetrate and destroy the phospholipid membrane of the virus. Among the cationic surfactants studied, didodecyldimethylammonium bromide was shown to be the most efficient as a disinfectant, its 50% effective concentration (EC50) being equal to 0.016 mM. Two surfactants (didodecyldimethylammonium bromide and benzalkonium chloride) can deactivate SARS-CoV-2 in as little as 5 s.<\/jats:p>","DOI":"10.3390\/ijms23126645","type":"journal-article","created":{"date-parts":[[2022,6,14]],"date-time":"2022-06-14T23:50:14Z","timestamp":1655250614000},"page":"6645","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":37,"title":["Cationic Surfactants as Disinfectants against SARS-CoV-2"],"prefix":"10.3390","volume":"23","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1162-118X","authenticated-orcid":false,"given":"Eduard V.","family":"Karamov","sequence":"first","affiliation":[{"name":"Gamaleya National Research Center for Epidemiology and Microbiology of the Russian Ministry of Health, 123098 Moscow, Russia"},{"name":"National Medical Research Center of Phthisiopulmonology and Infectious Diseases of the Russian Ministry of Health, 127473 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Viktor F.","family":"Larichev","sequence":"additional","affiliation":[{"name":"Gamaleya National Research Center for Epidemiology and Microbiology of the Russian Ministry of Health, 123098 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Galina V.","family":"Kornilaeva","sequence":"additional","affiliation":[{"name":"Gamaleya National Research Center for Epidemiology and Microbiology of the Russian Ministry of Health, 123098 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Irina T.","family":"Fedyakina","sequence":"additional","affiliation":[{"name":"Gamaleya National Research Center for Epidemiology and Microbiology of the Russian Ministry of Health, 123098 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ali S.","family":"Turgiev","sequence":"additional","affiliation":[{"name":"Gamaleya National Research Center for Epidemiology and Microbiology of the Russian Ministry of Health, 123098 Moscow, Russia"},{"name":"National Medical Research Center of Phthisiopulmonology and Infectious Diseases of the Russian Ministry of Health, 127473 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3019-5764","authenticated-orcid":false,"given":"Andrey V.","family":"Shibaev","sequence":"additional","affiliation":[{"name":"Physics Department, Moscow State University, 119991 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2846-0784","authenticated-orcid":false,"given":"Vyacheslav S.","family":"Molchanov","sequence":"additional","affiliation":[{"name":"Physics Department, Moscow State University, 119991 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1098-0255","authenticated-orcid":false,"given":"Olga E.","family":"Philippova","sequence":"additional","affiliation":[{"name":"Physics Department, Moscow State University, 119991 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexei R.","family":"Khokhlov","sequence":"additional","affiliation":[{"name":"Physics Department, Moscow State University, 119991 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,6,14]]},"reference":[{"key":"ref_1","unstructured":"Worldometer (2022, May 30). Coronavirus. Available online: https:\/\/www.worldometers.info\/coronavirus\/#countries."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"296","DOI":"10.1111\/tbed.13707","article-title":"Stability of SARS-CoV-2 and other coronaviruses in the environment and on common touch surfaces and the influence of climatic conditions: A review","volume":"68","author":"Aboubakr","year":"2021","journal-title":"Transbound. Emerg. Dis."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"E10","DOI":"10.1016\/S2666-5247(20)30003-3","article-title":"Stability of SARS-CoV-2 in different environmental conditions","volume":"1","author":"Chin","year":"2020","journal-title":"Lancet Microbe"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1564","DOI":"10.1056\/NEJMc2004973","article-title":"Aerosol and Surface Stability of SARS-CoV-2 as Compared with SARS-CoV-1","volume":"382","author":"Bushmaker","year":"2020","journal-title":"N. Engl. J. Med."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"142","DOI":"10.1016\/j.jhin.2020.11.023","article-title":"Alcohol-free hand sanitizer and other quaternary ammonium disinfectants quickly and effectively inactivate SARS-CoV-2","volume":"108","author":"Ogilvie","year":"2021","journal-title":"J. Hosp. Infect."},{"key":"ref_6","unstructured":"Gold, N.A., Mirza, T.M., and Avva, U. (2022). Alcohol Sanitizer, StatPearls Publishing. PMID: 30020626."},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Basak, D., and Deb, S. (2021). Sensitivity of SARS-CoV-2 towards alcohols: Potential for alcohol related toxicity in humans. Life, 11.","DOI":"10.3390\/life11121334"},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Vereshchagin, A.N., Frolov, N.A., Egorova, K.S., Seitkalieva, M.M., and Ananikov, V.P. (2021). Quaternary ammonium compounds (QACs) and ionic liquids (ILs) as biocides: From simple antiseptics to tunable antimicrobials. Int. J. Mol. Sci., 22.","DOI":"10.3390\/ijms22136793"},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Druvari, D., Antonopoulou, A., Lainioti, G.C., Vlamis-Gardikas, A., Bokias, G., and Kallitsis, J.K. (2021). Preparation of antimicrobial coatings from cross-linked copolymers containing quaternary dodecyl-ammonium compounds. Int. J. Mol. Sci., 22.","DOI":"10.3390\/ijms222413236"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1553","DOI":"10.1021\/acsinfecdis.0c00265","article-title":"Are Quaternary Ammonium Compounds, the Workhorse Disinfectants, Effective against Severe Acute Respiratory Syndrome-Coronavirus-2?","volume":"6","author":"Schrank","year":"2020","journal-title":"ACS Infect. Dis."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"104","DOI":"10.1007\/s11095-020-02842-8","article-title":"Repurposing quaternary ammonium compounds as potential treatments for COVID-19","volume":"37","author":"Baker","year":"2020","journal-title":"Pharm. Res."},{"key":"ref_12","first-page":"536","article-title":"The species Severe acute respiratory syndrome-related coronavirus: Classifying 2019-nCoV and naming it SARS-CoV-2","volume":"5","author":"Gorbalenya","year":"2000","journal-title":"Nat. Microbiol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1265","DOI":"10.1177\/00220345211029269","article-title":"Mouthwashes with CPC reduce the infectivity of SARS-CoV-2 variants in vitro","volume":"100","author":"Leon","year":"2021","journal-title":"J. Dent. Res."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"305","DOI":"10.1007\/s15010-020-01563-9","article-title":"Efficacy of commercial mouth-rinses on SARS-CoV-2 viral load in saliva: Randomized control trial in Singapore","volume":"49","author":"Seneviratne","year":"2021","journal-title":"Infection"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"449","DOI":"10.1006\/jcis.1997.4870","article-title":"Microemulsions with didodecyldimethylammonium bromide studied by neutron contrast variation","volume":"190","author":"Eastoe","year":"1997","journal-title":"J. Colloid Interface Sci."},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"McKeen, L. (2012). Introduction to food irradiation and medical sterilization. The Effect of Sterilization on Plastics and Elastomers, William Andrew Publishing. [3rd ed.].","DOI":"10.1016\/B978-1-4557-2598-4.00001-0"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1042-e1","DOI":"10.1016\/j.cmi.2021.04.009","article-title":"Disinfectant effectiveness against SARS-CoV-2 and influenza viruses present on human skin: Model-based evaluation","volume":"27","author":"Hirose","year":"2021","journal-title":"Clin. Microbiol. Infect."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"2418","DOI":"10.1038\/s41598-021-82148-w","article-title":"In vitro inactivation of SARS-CoV-2 by commonly used disinfection products and methods","volume":"11","author":"Guo","year":"2021","journal-title":"Sci. Rep."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"587","DOI":"10.1007\/s11743-012-1427-z","article-title":"Micellar and interfacial behavior of cationic benzalkonium chloride and nonionic polyoxyethylene alkyl ether based mixed surfactant systems","volume":"16","author":"Nandni","year":"2013","journal-title":"J. Surfact. Deterg."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"365","DOI":"10.1016\/j.jcis.2016.06.015","article-title":"A comparison of didodecyldimethylammonium bromide adsorbed at mica\/water and silica\/water interfaces using neutron reflection","volume":"478","author":"Griffin","year":"2016","journal-title":"J. Colloid Interface Sci."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"1480","DOI":"10.1126\/science.270.5241.1480","article-title":"Molecular organization of surfactants at solid-liquid interfaces","volume":"270","author":"Manne","year":"1995","journal-title":"Science"},{"key":"ref_22","first-page":"17","article-title":"Cationic emulsifiers in cosmetics","volume":"17","author":"Godfrey","year":"1966","journal-title":"J. Soc. Cosmet. Chem."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1016\/j.colsurfa.2004.12.038","article-title":"Effect of temperature on critical micelle concentration and thermodynamic behavior of dodecyldimethylethylammonium bromide and dodecyltrimethylammonium chloride in aqueous media","volume":"255","author":"Mehta","year":"2005","journal-title":"Colloids Surf. A Physicochem. Eng. Asp."},{"key":"ref_24","unstructured":"ChemBK (2022, May 23). Online Chemical Database for PC. Available online: https:\/\/www.chembk.com\/en\/chem\/DTAC."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1021\/bk-1988-0371.ch003","article-title":"In vitro test for effects of surfactants and formulations on permeability of plant cuticles","volume":"Volume 371","author":"Cross","year":"1988","journal-title":"Pesticide Formulations Innovations and Developments"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"2822","DOI":"10.1021\/ma951006p","article-title":"Interaction of hydrophobically modified poly(acrylic acid) hydrogels with ionic surfactants","volume":"29","author":"Philippova","year":"1996","journal-title":"Macromolecules"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"409","DOI":"10.1016\/S0966-7822(98)00030-6","article-title":"Two mechanisms of gel\/surfactant binding","volume":"6","author":"Philippova","year":"1998","journal-title":"Polym. Gels Netw."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"511","DOI":"10.1016\/j.jcis.2010.06.070","article-title":"Aggregation and adsorption behavior of cetylpyridinium chloride in aqueous sodium salicylate and sodium benzoate solutions","volume":"350","author":"Mukhim","year":"2010","journal-title":"J. Colloid Interface Sci."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1016\/0040-6031(90)80634-B","article-title":"Aqueous micellar solutions of some N-alkylnicotinamide and N-alkylpyridinium halide surfactants: Apparent and partial molar enthalpies","volume":"168","author":"Fisicaro","year":"1990","journal-title":"Thermochim. Acta"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1016\/S0927-7757(02)00404-1","article-title":"Hydrophile-lipophile balance and solubility parameter of cationic surfactants","volume":"214","author":"Proverbio","year":"2003","journal-title":"Colloids Surf. A Physicochem. Eng. Asp."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1515\/tsd-1992-290208","article-title":"Calculation of HLB values","volume":"29","author":"Sowada","year":"1992","journal-title":"Tenside Surf. Deterg."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"843","DOI":"10.2166\/wh.2020.108","article-title":"Chemical disinfectants of COVID-19: An overview","volume":"18","year":"2020","journal-title":"J. Water Health"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"2934","DOI":"10.1021\/la00060a012","article-title":"Theory of surfactant self-assembly: A predictive molecular thermodynamic approach","volume":"7","author":"Nagarajan","year":"1991","journal-title":"Langmuir"},{"key":"ref_34","unstructured":"Halva, H.S. (2001). Association colloids: Cmc a property to calculate. Handbook of Surfaces and Interfaces of Materials, Academic Press."},{"key":"ref_35","unstructured":"Kruglyakov, P.M. (2000). Hydrophile-Lipophile Balance of Surfactants and Solid Particles. Physicochemical Aspects and Applications, Elsevier."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1002\/jss.400040209","article-title":"The HLB dependency for detergent solubilization of hormonally sensitive adenylate cyclase","volume":"4","author":"Storm","year":"1976","journal-title":"J. Supramol. Struct."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"249","DOI":"10.1006\/jcis.1997.5316","article-title":"Electrokinetic studies on emulsions stabilized by ionic surfactants: The electroacoustophoretic behavior and estimation of Davies\u2019 HLB increments","volume":"198","author":"Ho","year":"1998","journal-title":"J. Colloid Interface Sci."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1016\/0009-3084(95)02456-S","article-title":"Solubilizing effects caused by alkyl pyridinium surfactants in phosphatidylcholine liposomes","volume":"77","author":"Parra","year":"1995","journal-title":"Chem. Phys. Lipids"},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Brandolini, M., Taddei, F., Marino, M.M., Grumiro, L., Scalcione, A., Turba, M.E., Gentilini, F., Fantini, M., Zannoli, S., and Dirani, G. (2021). Correlating qRT-PCR, dPCR and viral titration for the identification and quantification of SARS-CoV-2: A new approach for infection management. Viruses, 13.","DOI":"10.3390\/v13061022"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"96","DOI":"10.1038\/s41421-021-00340-8","article-title":"Isolation of a panel of ultra-potent human antibodies neutralizing SARS-CoV-2 and viral variants of concern","volume":"7","author":"Gorchakov","year":"2021","journal-title":"Cell Discov."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1007\/978-1-0716-2111-0_9","article-title":"Quantification of infectious SARS-CoV-2 by the 50% tissue culture infectious dose endpoint dilution assay","volume":"2452","author":"Bullen","year":"2022","journal-title":"Methods Mol. Biol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1038\/s41421-020-00191-9","article-title":"Survival of SARS-COV-2 under liquid medium, dry filter paper and acidic conditions","volume":"6","author":"Sun","year":"2020","journal-title":"Cell Discov."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1007\/s11262-021-01826-z","article-title":"Isolation and genetic characterization of SARS-CoV-2 from Indian patients in a single family without H\/O travel abroad","volume":"57","author":"Shrivastava","year":"2021","journal-title":"Virus Genes"},{"key":"ref_44","unstructured":"Carter, J., and Saunders, V. (2007). Virology: Principles and Applications, John Wiley & Sons."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"611","DOI":"10.1016\/S0960-894X(01)81162-8","article-title":"5-(3-carboxymethoxyphenyl)-2-(4,5-dimethylthiazolyl)-3-(4-sulfophenyl)tetrazolium, inner salt (MTS) and related analogs of 3-(4,5-dimethylthiazolyl)-2,5-diphenyltetrazolium bromide (MTT) reducing to purple water-soluble formazans as cell-viability indicators","volume":"1","author":"Barltrop","year":"1991","journal-title":"Bioorg. Med. Chem. Lett."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"474","DOI":"10.1006\/abbi.1993.1311","article-title":"Characterization of the cellular reduction of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT): Subcellular localization, substrate dependence, and involvement of mitochondrial electron transport in MTT reduction","volume":"303","author":"Berridge","year":"1993","journal-title":"Arch. Biochem. Biophys."},{"key":"ref_47","first-page":"24","article-title":"The infectious cycle","volume":"Volume 1","author":"Flint","year":"2015","journal-title":"Principles of Virology"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"480","DOI":"10.1007\/BF01863914","article-title":"Beitrag zur kollektiven Behandlung pharmakologischer Reihenversuche","volume":"162","year":"1931","journal-title":"Naunyn-Schmiedebergs Arch. F\u00fcr Exp. Pathol. Und Pharmakol."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"2039","DOI":"10.1021\/ja00449a004","article-title":"Environmental effects on vibronic band intensities in pyrene monomer fluorescence and their application in studies of micellar systems","volume":"99","author":"Kalyanasundaram","year":"1977","journal-title":"J. Am. Chem. Soc."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1016\/S0021-9673(02)01145-7","article-title":"Molecular mass characterization of polymers with strongly interacting groups using gel permeation chromatography\u2013light scattering detection","volume":"976","author":"Blagodatskikh","year":"2002","journal-title":"J. Chrom. A"},{"key":"ref_51","unstructured":"(2022, June 01). Regulatory Framework | Disinfection & Sterilization Guidelines | Guidelines Library | Infection Control | CDC, Available online: https:\/\/www.cdc.gov\/infectioncontrol\/guidelines\/disinfection\/glossary.html#V."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1007\/s11084-010-9194-1","article-title":"Defining life: The virus viewpoint","volume":"40","author":"Forterre","year":"2010","journal-title":"Orig. Life Evol. Biosph."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1016\/j.shpsc.2016.02.016","article-title":"Are viruses alive? The replicator paradigm sheds decisive light on an old but misguided question","volume":"59","author":"Koonin","year":"2016","journal-title":"Stud. Hist. Philos. Biol. Biomed. 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