{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T13:17:19Z","timestamp":1790774239698,"version":"4.1.0"},"reference-count":55,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2022,6,16]],"date-time":"2022-06-16T00:00:00Z","timestamp":1655337600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Natural Science Foundation of China","award":["32172951"],"award-info":[{"award-number":["32172951"]}]},{"name":"National Natural Science Foundation of China","award":["2018YFD0900400"],"award-info":[{"award-number":["2018YFD0900400"]}]},{"name":"National Natural Science Foundation of China","award":["AHSC202002"],"award-info":[{"award-number":["AHSC202002"]}]},{"DOI":"10.13039\/501100012166","name":"National Key R&amp;D Program of China","doi-asserted-by":"publisher","award":["32172951"],"award-info":[{"award-number":["32172951"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Key R&amp;D Program of China","doi-asserted-by":"publisher","award":["2018YFD0900400"],"award-info":[{"award-number":["2018YFD0900400"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Key R&amp;D Program of China","doi-asserted-by":"publisher","award":["AHSC202002"],"award-info":[{"award-number":["AHSC202002"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Anhui Province Key Laboratory of Aquaculture &amp; Stock Enhancement","award":["32172951"],"award-info":[{"award-number":["32172951"]}]},{"name":"Anhui Province Key Laboratory of Aquaculture &amp; Stock Enhancement","award":["2018YFD0900400"],"award-info":[{"award-number":["2018YFD0900400"]}]},{"name":"Anhui Province Key Laboratory of Aquaculture &amp; Stock Enhancement","award":["AHSC202002"],"award-info":[{"award-number":["AHSC202002"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Regulation of food intake is associated with nutrient-sensing systems and the expression of appetite neuropeptides. Nutrient-sensing systems generate the capacity to sense nutrient availability to maintain energy and metabolism homeostasis. Appetite neuropeptides are prominent factors that are essential for regulating the appetite to adapt energy status. However, the link between the expression of appetite neuropeptides and nutrient-sensing systems remains debatable in carnivorous fish. Here, with intracerebroventricular (ICV) administration of six essential amino acids (lysine, methionine, tryptophan, arginine, phenylalanine, or threonine) performed in mandarin fish (Siniperca chuatsi), we found that lysine and methionine are the feeding-stimulating amino acids other than the reported valine, and found a key appetite neuropeptide, neuropeptide Y (NPY), mainly contributes to the regulatory role of the essential amino acids on food intake. With the brain cells of mandarin fish cultured in essential amino acid deleted medium (lysine, methionine, histidine, valine, or leucine), we showed that only lysine deprivation activated the general control nonderepressible 2 (GCN2) signaling pathway, elevated \u03b1 subunit of eukaryotic translation initiation factor 2 (eIF2\u03b1) phosphorylation, increased activating transcription factor 4 (ATF4) protein expression, and finally induced transcription of npy. Furthermore, pharmacological inhibition of GCN2 and eIF2\u03b1 phosphorylation signaling by GCN2iB or ISRIB, effectively blocked the transcriptional induction of npy in lysine deprivation. Overall, these findings could provide a better understanding of the GCN2 signaling pathway involved in food intake control by amino acids.<\/jats:p>","DOI":"10.3390\/ijms23126727","type":"journal-article","created":{"date-parts":[[2022,6,17]],"date-time":"2022-06-17T01:48:12Z","timestamp":1655430492000},"page":"6727","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Lysine Deprivation Regulates Npy Expression via GCN2 Signaling Pathway in Mandarin Fish (Siniperca chuatsi)"],"prefix":"10.3390","volume":"23","author":[{"given":"Jia-Ming","family":"Zou","sequence":"first","affiliation":[{"name":"College of Fisheries, Chinese Perch Research Center, Huazhong Agricultural University, Wuhan 430070, China"},{"name":"Engineering Research Center of Green development for Conventional Aquatic Biological Industry in the Yangtze River Economic Belt, Ministry of Education, Huazhong Agricultural University, Wuhan 430070, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiang-Sheng","family":"Zhu","sequence":"additional","affiliation":[{"name":"College of Fisheries, Chinese Perch Research Center, Huazhong Agricultural University, Wuhan 430070, China"},{"name":"Engineering Research Center of Green development for Conventional Aquatic Biological Industry in the Yangtze River Economic Belt, Ministry of Education, Huazhong Agricultural University, Wuhan 430070, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui","family":"Liang","sequence":"additional","affiliation":[{"name":"College of Fisheries, Chinese Perch Research Center, Huazhong Agricultural University, Wuhan 430070, China"},{"name":"Engineering Research Center of Green development for Conventional Aquatic Biological Industry in the Yangtze River Economic Belt, Ministry of Education, Huazhong Agricultural University, Wuhan 430070, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hai-Lin","family":"Lu","sequence":"additional","affiliation":[{"name":"College of Fisheries, Chinese Perch Research Center, Huazhong Agricultural University, Wuhan 430070, China"},{"name":"Engineering Research Center of Green development for Conventional Aquatic Biological Industry in the Yangtze River Economic Belt, Ministry of Education, Huazhong Agricultural University, Wuhan 430070, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xu-Fang","family":"Liang","sequence":"additional","affiliation":[{"name":"College of Fisheries, Chinese Perch Research Center, Huazhong Agricultural University, Wuhan 430070, China"},{"name":"Engineering Research Center of Green development for Conventional Aquatic Biological Industry in the Yangtze River Economic Belt, Ministry of Education, Huazhong Agricultural University, Wuhan 430070, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shan","family":"He","sequence":"additional","affiliation":[{"name":"College of Fisheries, Chinese Perch Research Center, Huazhong Agricultural University, Wuhan 430070, China"},{"name":"Engineering Research Center of Green development for Conventional Aquatic Biological Industry in the Yangtze River Economic Belt, Ministry of Education, Huazhong Agricultural University, Wuhan 430070, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,6,16]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"367","DOI":"10.1038\/nrn3745","article-title":"Neurobiology of food intake in health and disease","volume":"15","author":"Morton","year":"2014","journal-title":"Nat. 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