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TRIM28 contributes to physiological functions such as cell growth and differentiation. In the chronic myeloid leukemia cell line K562, we edited TRIM28 using CRISPR\/Cas9 technology, and the complete and partial knockout (KO) cell clones were obtained and confirmed using quantitative droplet digital PCR (ddPCR) technology. The amplicon sequencing demonstrated no off-target effects in our gene editing experiments. The TRIM28 KO cells grew slowly and appeared red, seeming to have a tendency towards erythroid differentiation. To understand how TRIM28 controls K562 cell proliferation and differentiation, transcriptome profiling analysis was performed in wild-type and KO cells to identify TRIM28-regulated genes. Some of the RNAs that encode the proteins regulating the cell cycle were increased (such as p21) or decreased (such as cyclin D2) in TRIM28 KO cell clones; a tumor marker, the MAGE (melanoma antigen) family, which is involved in cell proliferation was reduced. Moreover, we found that knockout of TRIM28 can induce miR-874 expression to downregulate MAGEC2 mRNA via post-transcriptional regulation. The embryonic epsilon-globin gene was significantly increased in TRIM28 KO cell clones through the downregulation of transcription repressor SOX6. Taken together, we provide evidence to demonstrate the regulatory network of TRIM28-mediated cell growth and erythroid differentiation in K562 leukemia cells.<\/jats:p>","DOI":"10.3390\/ijms23126839","type":"journal-article","created":{"date-parts":[[2022,6,21]],"date-time":"2022-06-21T01:43:27Z","timestamp":1655775807000},"page":"6839","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Generation of TRIM28 Knockout K562 Cells by CRISPR\/Cas9 Genome Editing and Characterization of TRIM28-Regulated Gene Expression in Cell Proliferation and Hemoglobin Beta Subunits"],"prefix":"10.3390","volume":"23","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1985-2428","authenticated-orcid":false,"given":"Yao-Jen","family":"Chang","sequence":"first","affiliation":[{"name":"Institute of Biological Chemistry, Academia Sinica, Taipei 11529, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhifu","family":"Kang","sequence":"additional","affiliation":[{"name":"Graduate Institute of Biochemical Sciences, College of Life Science, National Taiwan University, Taipei 10617, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiayuan","family":"Bei","sequence":"additional","affiliation":[{"name":"Graduate Institute of Biochemical Sciences, College of Life Science, National Taiwan University, Taipei 10617, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shu-Jen","family":"Chou","sequence":"additional","affiliation":[{"name":"Institute of Plant and Microbial Biology, Academia Sinica, Taipei 11529, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mei-Yeh Jade","family":"Lu","sequence":"additional","affiliation":[{"name":"Biodiversity Research Center, Academia Sinica, Taipei 11529, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3803-6047","authenticated-orcid":false,"given":"Yu-Lun","family":"Su","sequence":"additional","affiliation":[{"name":"Graduate Institute of Biochemical Sciences, College of Life Science, National Taiwan University, Taipei 10617, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7777-2090","authenticated-orcid":false,"given":"Sheng-Wei","family":"Lin","sequence":"additional","affiliation":[{"name":"Institute of Biological Chemistry, Academia Sinica, Taipei 11529, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hsin-Hui","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Division of Pediatric Immunology and Nephrology, Taipei Veterans General Hospital, Taipei 11217, Taiwan"},{"name":"Department of Pediatrics, School of Medicine, College of Medicine, National Yang Ming Chiao Tung University, Taipei 112304, Taiwan"},{"name":"Institute of Emergency and Critical Care Medicine, College of Medicine, National Yang Ming Chiao Tung University, Taipei 112304, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7542-4663","authenticated-orcid":false,"given":"Steven","family":"Lin","sequence":"additional","affiliation":[{"name":"Institute of Biological Chemistry, Academia Sinica, Taipei 11529, Taiwan"},{"name":"Graduate Institute of Biochemical Sciences, College of Life Science, National Taiwan University, Taipei 10617, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ching-Jin","family":"Chang","sequence":"additional","affiliation":[{"name":"Institute of Biological Chemistry, Academia Sinica, Taipei 11529, Taiwan"},{"name":"Graduate Institute of Biochemical Sciences, College of Life Science, National Taiwan University, Taipei 10617, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,6,20]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"2067","DOI":"10.1101\/gad.10.16.2067","article-title":"KAP-1, a novel corepressor for the highly conserved KRAB repression domain","volume":"10","author":"Friedman","year":"1996","journal-title":"Genes Dev."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1139","DOI":"10.1006\/jmbi.1999.3402","article-title":"Reconstitution of the KRAB-KAP-1 repressor complex: A model system for defining the molecular anatomy of RING-B box-coiled-coil domain-mediated protein-protein interactions","volume":"295","author":"Peng","year":"2000","journal-title":"J. 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