{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T23:24:52Z","timestamp":1790810692545,"version":"4.1.0"},"reference-count":28,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2022,6,20]],"date-time":"2022-06-20T00:00:00Z","timestamp":1655683200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31570242"],"award-info":[{"award-number":["31570242"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["[2020]4004"],"award-info":[{"award-number":["[2020]4004"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["2020XM03"],"award-info":[{"award-number":["2020XM03"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Guizhou Provincial Academician Workstation of Microbiology and Health","award":["31570242"],"award-info":[{"award-number":["31570242"]}]},{"name":"Guizhou Provincial Academician Workstation of Microbiology and Health","award":["[2020]4004"],"award-info":[{"award-number":["[2020]4004"]}]},{"name":"Guizhou Provincial Academician Workstation of Microbiology and Health","award":["2020XM03"],"award-info":[{"award-number":["2020XM03"]}]},{"name":"Guizhou Tobacco Company","award":["31570242"],"award-info":[{"award-number":["31570242"]}]},{"name":"Guizhou Tobacco Company","award":["[2020]4004"],"award-info":[{"award-number":["[2020]4004"]}]},{"name":"Guizhou Tobacco Company","award":["2020XM03"],"award-info":[{"award-number":["2020XM03"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Arabidopsis AGD2 (Aberrant Growth and Death2) and its close homolog ALD1 (AGD2-like defense response protein 1) have divergent roles in plant defense. We previously reported that modulation of salicylic acid (SA) contents by ALD1 affects numbers of nodules produced by Lotus japonicus, but AGD2\u2032s role in leguminous plants remains unclear. A combination of enzymatic analysis and biological characterization of genetic materials was used to study the function of AGD2 (LjAGD2a and LjAGD2b) in L. japonicus. Both LjAGD2a and LjAGD2b could complement dapD and dapE mutants of Escherichia coli and had aminotransferase activity in vitro. ljagd2 plants, with insertional mutations of LjAGD2, had delayed flowering times and reduced seed weights. In contrast, overexpression of LjAGD2a in L. japonicus induced early flowering, with increases in seed and flower sizes, but reductions in pollen fertility and seed setting rates. Additionally, ljagd2a mutation resulted in increased expression of nodulin genes and corresponding increases in infection threads and nodule numbers following inoculation with Rhizobium. Changes in expression of LjAGD2a in L. japonicus also affected endogenous SA contents and hence resistance to pathogens. Our results indicate that LjAGD2a functions as an LL-DAP aminotransferase and plays important roles in plant development. Moreover, LjAGD2a activates defense signaling via the Lys synthesis pathway, thereby participating in legume\u2013microbe interaction.<\/jats:p>","DOI":"10.3390\/ijms23126863","type":"journal-article","created":{"date-parts":[[2022,6,21]],"date-time":"2022-06-21T01:43:27Z","timestamp":1655775807000},"page":"6863","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Roles of AGD2a in Plant Development and Microbial Interactions of Lotus japonicus"],"prefix":"10.3390","volume":"23","author":[{"given":"Mingchao","family":"Huang","sequence":"first","affiliation":[{"name":"Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement & Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China"},{"name":"University of Chinese Academy of Sciences, Beijing 100049, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mengru","family":"Yuan","sequence":"additional","affiliation":[{"name":"Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement & Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China"},{"name":"University of Chinese Academy of Sciences, Beijing 100049, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chunyu","family":"Sun","sequence":"additional","affiliation":[{"name":"Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement & Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China"},{"name":"University of Chinese Academy of Sciences, Beijing 100049, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meiru","family":"Li","sequence":"additional","affiliation":[{"name":"Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement & Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pingzhi","family":"Wu","sequence":"additional","affiliation":[{"name":"Key Laboratory of South Subtropical Fruit Biology and Genetic Resource Utilization, Ministry of Agriculture\/Key Laboratory of Tropical and Subtropical Fruit Tree Research of Guangdong Province, Institution of Fruit Tree Research, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huawu","family":"Jiang","sequence":"additional","affiliation":[{"name":"Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement & Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guojiang","family":"Wu","sequence":"additional","affiliation":[{"name":"Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement & Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0118-4265","authenticated-orcid":false,"given":"Yaping","family":"Chen","sequence":"additional","affiliation":[{"name":"Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement & Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,6,20]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1111\/tpj.14037","article-title":"L-lysine metabolism to N-hydroxypipecolic acid: An integral immune-activating pathway in plants","volume":"96","author":"Hartmann","year":"2018","journal-title":"Plant J."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"324","DOI":"10.1016\/S1360-1385(00)01688-5","article-title":"Regulation of lysine catabolism in higher plants","volume":"5","author":"Arruda","year":"2000","journal-title":"Trends Plant Sci."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"239","DOI":"10.3389\/fpls.2012.00239","article-title":"Quinolizidine alkaloid biosynthesis: Recent advances and future prospects","volume":"3","author":"Bunsupa","year":"2012","journal-title":"Front. Plant Sci."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"587","DOI":"10.3389\/fpls.2020.00587","article-title":"Lysine catabolism through the saccharopine pathway: Enzymes and intermediates involved in plant responses to abiotic and biotic stress","volume":"11","author":"Arruda","year":"2020","journal-title":"Front. Plant Sci."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"456.e16","DOI":"10.1016\/j.cell.2018.02.049","article-title":"Flavin monooxygenase-generated N-hydroxypipecolic acid is a critical element of plant systemic immunity","volume":"173","author":"Hartmann","year":"2018","journal-title":"Cell"},{"key":"ref_6","first-page":"E4920","article-title":"N-hydroxy-pipecolic acid is a mobile metabolite that induces systemic disease resistance in Arabidopsis","volume":"115","author":"Chen","year":"2018","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_7","first-page":"6735209","article-title":"De novo assembly and comparative transcriptome analysis provide insight into lysine biosynthesis in Toona sinensis Roem","volume":"2016","author":"Zhang","year":"2016","journal-title":"Int. J. Genom."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"292","DOI":"10.1104\/pp.105.072629","article-title":"An LL-diaminopimelate aminotransferase defines a novel variant of the lysine biosynthesis pathway in plants","volume":"140","author":"Hudson","year":"2006","journal-title":"Plant Physiol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"2603","DOI":"10.1105\/tpc.16.00486","article-title":"Characterization of a pipecolic acid biosynthesis pathway required for systemic acquired resistance","volume":"28","author":"Ding","year":"2016","journal-title":"Plant Cell"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1046\/j.0960-7412.2001.1075umedoc.x","article-title":"The Arabidopsis aberrant growth and death2 mutant shows resistance to Pseudomonas syringae and reveals a role for NPR1 in suppressing hypersensitive cell death","volume":"27","author":"Rate","year":"2001","journal-title":"Plant J."},{"key":"ref_11","first-page":"27","article-title":"Biosynthesis of lysine in plants: Evidence for a variant of the known bacterial pathways","volume":"1721","author":"Hudson","year":"2005","journal-title":"BBA"},{"key":"ref_12","first-page":"5489","article-title":"An L,L-diaminopimelate aminotransferase mutation leads to metabolic shifts and growth inhibition in Arabidopsis","volume":"69","author":"Cavalcanti","year":"2018","journal-title":"J. Exp. Bot."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1034","DOI":"10.1111\/jipb.12211","article-title":"Knockdown of LjALD1, AGD2-like defense response protein 1, influences plant growth and nodulation in Lotus japonicus","volume":"56","author":"Chen","year":"2014","journal-title":"J. Integr. Plant Biol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"353","DOI":"10.1105\/tpc.019372","article-title":"Divergent roles in Arabidopsis thaliana development and defense of two homologous genes, aberrant growth and death2 and AGD2-LIKE DEFENSE RESPONSE PROTEIN1, encoding novel aminotransferases","volume":"16","author":"Song","year":"2004","journal-title":"Plant Cell"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"455","DOI":"10.1094\/MPMI-06-14-0187-R","article-title":"ALD1 regulates basal immune components and early inducible defense responses in Arabidopsis","volume":"28","author":"Cecchini","year":"2015","journal-title":"Mol. Plant Microbe Interact."},{"key":"ref_16","first-page":"161","article-title":"5\u2014Advances in the biochemistry of amino acid biosynthesis","volume":"Volume 16","author":"Miflin","year":"1990","journal-title":"Intermediary Nitrogen Metabolism"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1146\/annurev.arplant.53.091401.110929","article-title":"New insights into the regulation and functional significance of lysine metabolism in plants","volume":"53","author":"Galili","year":"2002","journal-title":"Annu. Rev. Plant Biol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"928","DOI":"10.3389\/fpls.2020.00928","article-title":"Connections between amino acid metabolisms in plants: Lysine as an example","volume":"11","author":"Yang","year":"2020","journal-title":"Front. Plant Sci."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1473","DOI":"10.1104\/pp.106.080986","article-title":"Effects of endogenous salicylic acid on nodulation in the model legumes Lotus japonicus and Medicago truncatula","volume":"141","author":"Stacey","year":"2006","journal-title":"Plant Physiol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1104\/pp.103.037168","article-title":"Lysine metabolism is concurrently regulated by synthesis and catabolism in both reproductive and vegetative tissues","volume":"135","author":"Zhu","year":"2004","journal-title":"Plant Physiol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"306","DOI":"10.1111\/tpj.13243","article-title":"The LORE1 insertion mutant resource","volume":"88","author":"Malolepszy","year":"2016","journal-title":"Plant J."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"39447","DOI":"10.1038\/srep39447","article-title":"Lotus Base: An integrated information portal for the model legume Lotus japonicus","volume":"6","author":"Mun","year":"2016","journal-title":"Sci. Rep."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1016\/j.jprot.2017.01.017","article-title":"Proteolysis of chloroplast proteins is responsible for accumulation of free amino acids in dark-treated tea (Camellia sinensis) leaves","volume":"157","author":"Chen","year":"2017","journal-title":"J. Proteom."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"2731","DOI":"10.1093\/molbev\/msr121","article-title":"MEGA5: Molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods","volume":"28","author":"Tamura","year":"2011","journal-title":"Mol. Biol. Evol."},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Tian, L., Liu, L., Yin, Y., Huang, M., Chen, Y., Xu, X., Wu, P., Li, M., Wu, G., and Jiang, H. (2017). Heterogeneity in the expression and subcellular localization of POLYOL\/MONOSACCHARIDE TRANSPORTER genes in Lotus japonicus. PLoS ONE, 12.","DOI":"10.1371\/journal.pone.0185269"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"311","DOI":"10.1016\/j.plantsci.2019.03.011","article-title":"The role of endogenous thiamine produced via THIC in root nodule symbiosis in Lotus japonicus","volume":"283","author":"Yin","year":"2019","journal-title":"Plant Sci."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1315","DOI":"10.3389\/fpls.2016.01315","article-title":"Overexpression of the starch phosphorylase-like gene (PHO3) in Lotus japonicus has a profound effect on the growth of plants and reduction of transitory starch accumulation","volume":"7","author":"Qin","year":"2016","journal-title":"Front. Plant Sci."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"830900","DOI":"10.3389\/fmicb.2022.830900","article-title":"Complete genome sequence analysis of Ralstonia solanacearum strain PeaFJ1 provides insights into its strong virulence in peanut plants","volume":"13","author":"Tan","year":"2022","journal-title":"Front Microbiol."}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/23\/12\/6863\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T23:35:59Z","timestamp":1760139359000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/23\/12\/6863"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,6,20]]},"references-count":28,"journal-issue":{"issue":"12","published-online":{"date-parts":[[2022,6]]}},"alternative-id":["ijms23126863"],"URL":"https:\/\/doi.org\/10.3390\/ijms23126863","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,6,20]]}}}