{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T03:44:13Z","timestamp":1785815053914,"version":"3.56.0"},"reference-count":90,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2022,6,20]],"date-time":"2022-06-20T00:00:00Z","timestamp":1655683200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"the S\u00e3o Paulo Research Foundation (FAPESP)","award":["2018\/05181-7"],"award-info":[{"award-number":["2018\/05181-7"]}]},{"name":"the S\u00e3o Paulo Research Foundation (FAPESP)","award":["2019\/09741-0"],"award-info":[{"award-number":["2019\/09741-0"]}]},{"name":"the S\u00e3o Paulo Research Foundation (FAPESP)","award":["2018\/08631-3"],"award-info":[{"award-number":["2018\/08631-3"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>\u03b3\u03b4T cells mature in the human thymus, and mainly produce IL-17A or IFN-\u03b3, but can also produce IL-22 and modulate a variety of immune responses. Here, we aimed to evaluate whether IgG from AD patients (AD IgG) can functionally modulate thymic nonatopic \u03b3\u03b4T cells. Thymic tissues were obtained from 12 infants who had not had an atopic history. Thymocytes were cultured in mock condition, or in the presence of either AD IgG or therapeutic intravenous IgG (IVIg). Following these treatments, intracellular cytokine production, phenotype, and microRNA expression profiles were investigated. AD IgG could downregulate \u03b14\u03b27, upregulate CLA, and induce the production of IFN-\u03b3, IL-17, and IL-22 in \u03b3\u03b4T cells. Although both AD IgG and IVIg could directly interact with \u03b3\u03b4T cell membranes, AD IgG could reduce \u03b3\u03b4T cell apoptosis. AD IgG could upregulate nine miRNAs compared to IVIg, and six when compared to the mock condition. In parallel, some miRNAs were downregulated. Target gene prediction and functional analysis indicated that some target genes were enriched in the negative regulation of cellular transcription. This study shows that AD IgG influences the production of IL-17 and IL-22 by intrathymic nonatopic \u03b3\u03b4T cells, and demonstrates epigenetic implications mediated by miRNAs.<\/jats:p>","DOI":"10.3390\/ijms23126872","type":"journal-article","created":{"date-parts":[[2022,6,21]],"date-time":"2022-06-21T01:43:27Z","timestamp":1655775807000},"page":"6872","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["IgG from Adult Atopic Dermatitis (AD) Patients Induces Nonatopic Neonatal Thymic Gamma\u2013Delta T Cells (\u03b3\u03b4T) to Acquire IL-22\/IL-17 Secretion Profile with Skin-Homing Properties and Epigenetic Implications Mediated by miRNA"],"prefix":"10.3390","volume":"23","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9628-9184","authenticated-orcid":false,"given":"Beatriz Oliveira","family":"Fagundes","sequence":"first","affiliation":[{"name":"Laboratory of Medical Investigation LIM-56, Division of Dermatology, Medical School, University of Sao Paulo, Sao Paulo 05403-000, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thamires Rodrigues","family":"de Sousa","sequence":"additional","affiliation":[{"name":"Laboratory of Medical Investigation LIM-56, Division of Dermatology, Medical School, University of Sao Paulo, Sao Paulo 05403-000, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrezza","family":"Nascimento","sequence":"additional","affiliation":[{"name":"Post-Graduation Program in Translational Medicine, Federal University of Sao Paulo, Sao Paulo 04039-002, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8246-3126","authenticated-orcid":false,"given":"Lorena Abreu","family":"Fernandes","sequence":"additional","affiliation":[{"name":"Post-Graduation Program in Translational Medicine, Federal University of Sao Paulo, Sao Paulo 04039-002, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F\u00e1bio da Ressurei\u00e7\u00e3o","family":"Sgnotto","sequence":"additional","affiliation":[{"name":"Division of Hematology, Medical School, University of Sao Paulo, Sao Paulo 05403-000, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2807-1404","authenticated-orcid":false,"given":"Raquel Le\u00e3o","family":"Orfali","sequence":"additional","affiliation":[{"name":"Laboratory of Medical Investigation LIM-56, Division of Dermatology, Medical School, University of Sao Paulo, Sao Paulo 05403-000, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Val\u00e9ria","family":"Aoki","sequence":"additional","affiliation":[{"name":"Laboratory of Medical Investigation LIM-56, Division of Dermatology, Medical School, University of Sao Paulo, Sao Paulo 05403-000, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alberto Jos\u00e9 da Silva","family":"Duarte","sequence":"additional","affiliation":[{"name":"Laboratory of Medical Investigation LIM-56, Division of Dermatology, Medical School, University of Sao Paulo, Sao Paulo 05403-000, Brazil"},{"name":"Division of Pathology, Medical School, University of Sao Paulo, Sao Paulo 05403-000, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sabri Saeed","family":"Sanabani","sequence":"additional","affiliation":[{"name":"Laboratory of Medical Investigation LIM-56, Division of Dermatology, Medical School, University of Sao Paulo, Sao Paulo 05403-000, Brazil"},{"name":"Laboratory of Medical Investigation LIM-03, Division of Pathology, Medical School, University of Sao Paulo, Sao Paulo 05403-000, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6092-8394","authenticated-orcid":false,"given":"Jefferson Russo","family":"Victor","sequence":"additional","affiliation":[{"name":"Laboratory of Medical Investigation LIM-56, Division of Dermatology, Medical School, University of Sao Paulo, Sao Paulo 05403-000, Brazil"},{"name":"Faculdades Metropolitanas Unidas (FMU), Health Sciences School, Sao Paulo 04505-002, Brazil"},{"name":"Medical School, Universidade Santo Amaro (UNISA), Sao Paulo 04829-300, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,6,20]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"223","DOI":"10.3109\/08830185.2013.783831","article-title":"Gammadelta T cells: Innately adaptive immune cells?","volume":"32","author":"Ferreira","year":"2013","journal-title":"Int. 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