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In order to shed some light upon the transcriptional profile of skin melanoma, we compared the gene expression of six independent tumours (all progressed towards metastatic disease and with wild type BRAF) to the expression profile of non-dysplastic melanocytes (considered as a healthy control) in a pilot study. Paraffin-embedded samples were manually micro-dissected to obtain enriched samples, and then, RNA was extracted and analysed through a microarray-based approach. An exhaustive bioinformatics analysis was performed to identify differentially expressed transcripts between the two groups, as well as enriched functional terms. Overall, 50 up- and 19 downregulated transcripts were found to be significantly changed in the tumour compared to the control tissue. Among the upregulated transcripts, the majority belonged to the immune response group and to the proteasome, while most of the downregulated genes were related to cytosolic ribosomes. A Gene Set Enrichment Analysis (GSEA), along with the RNA-Seq data retrieved from the TCGA\/GTEx databases, confirmed the general trend of downregulation affecting cytoribosome proteins. In contrast, transcripts coding for mitoribosome proteins showed the opposite trend.<\/jats:p>","DOI":"10.3390\/ijms23136898","type":"journal-article","created":{"date-parts":[[2022,6,21]],"date-time":"2022-06-21T21:55:19Z","timestamp":1655848519000},"page":"6898","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["The Transcriptional Landscape of BRAF Wild Type Metastatic Melanoma: A Pilot Study"],"prefix":"10.3390","volume":"23","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1531-8973","authenticated-orcid":false,"given":"Elena","family":"Lastraioli","sequence":"first","affiliation":[{"name":"Department of Experimental and Clinical Medicine, University of Florence, Viale GB Morgagni 50, 50134 Florence, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3084-0380","authenticated-orcid":false,"given":"Federico Alessandro","family":"Ruffinatti","sequence":"additional","affiliation":[{"name":"Department of Life Sciences and Systems Biology, University of Torino, Via Accademia Albertina 13, 10123 Torino, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Giacomo","family":"Bagni","sequence":"additional","affiliation":[{"name":"Department of Experimental and Clinical Medicine, University of Florence, Viale GB Morgagni 50, 50134 Florence, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2568-5694","authenticated-orcid":false,"given":"Luca","family":"Visentin","sequence":"additional","affiliation":[{"name":"Department of Life Sciences and Systems Biology, University of Torino, Via Accademia Albertina 13, 10123 Torino, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Francesco","family":"di Costanzo","sequence":"additional","affiliation":[{"name":"Medical Oncology Unit, Azienda Ospedaliero-Universitaria Careggi, Largo Brambilla 3, 50134 Florence, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9247-4446","authenticated-orcid":false,"given":"Luca","family":"Munaron","sequence":"additional","affiliation":[{"name":"Department of Life Sciences and Systems Biology, University of Torino, Via Accademia Albertina 13, 10123 Torino, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Annarosa","family":"Arcangeli","sequence":"additional","affiliation":[{"name":"Department of Experimental and Clinical Medicine, University of Florence, Viale GB Morgagni 50, 50134 Florence, Italy"},{"name":"Complex Dynamics Study Centre (CSDC), University of Florence, 50100 Florence, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,6,21]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"e4070","DOI":"10.1097\/MD.0000000000004070","article-title":"Risk of skin cancer in patients with diabetes mellitus","volume":"95","author":"Tseng","year":"2016","journal-title":"Medicine"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"3711","DOI":"10.18632\/oncotarget.1987","article-title":"Melanoma cell therapy: Endothelial progenitor cells as shuttle of the MMP12 uPAR-degrading enzyme","volume":"5","author":"Laurenzana","year":"2014","journal-title":"Oncotarget"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"278","DOI":"10.3389\/fendo.2017.00278","article-title":"Circulating endothelial progenitor cells in type 1 diabetic patients: Relation with patients\u2019 age and disease duration","volume":"8","author":"Arcangeli","year":"2017","journal-title":"Front. 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