{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T06:27:19Z","timestamp":1785824839425,"version":"3.56.0"},"reference-count":45,"publisher":"MDPI AG","issue":"13","license":[{"start":{"date-parts":[[2022,6,22]],"date-time":"2022-06-22T00:00:00Z","timestamp":1655856000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004281","name":"Polish National Science Centre","doi-asserted-by":"publisher","award":["2016\/23\/B\/NZ6\/02536"],"award-info":[{"award-number":["2016\/23\/B\/NZ6\/02536"]}],"id":[{"id":"10.13039\/501100004281","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004281","name":"Polish National Science Centre","doi-asserted-by":"publisher","award":["POWR.03.02.00-00-1028\/17-00"],"award-info":[{"award-number":["POWR.03.02.00-00-1028\/17-00"]}],"id":[{"id":"10.13039\/501100004281","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000782","name":"ESF","doi-asserted-by":"publisher","award":["2016\/23\/B\/NZ6\/02536"],"award-info":[{"award-number":["2016\/23\/B\/NZ6\/02536"]}],"id":[{"id":"10.13039\/501100000782","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000782","name":"ESF","doi-asserted-by":"publisher","award":["POWR.03.02.00-00-1028\/17-00"],"award-info":[{"award-number":["POWR.03.02.00-00-1028\/17-00"]}],"id":[{"id":"10.13039\/501100000782","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Mossakowski Medical Research Institute, Warsaw, Poland","award":["2016\/23\/B\/NZ6\/02536"],"award-info":[{"award-number":["2016\/23\/B\/NZ6\/02536"]}]},{"name":"Mossakowski Medical Research Institute, Warsaw, Poland","award":["POWR.03.02.00-00-1028\/17-00"],"award-info":[{"award-number":["POWR.03.02.00-00-1028\/17-00"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Mononegavirales is an order of viruses with a genome in the form of a non-segmented negative-strand RNA that encodes several proteins. The functional polymerase complex of these viruses is composed of two proteins: a large protein (L) and a phosphoprotein (P). The replication of viruses from this order depends on Hsp90 chaperone activity. Previous studies have demonstrated that Hsp90 inhibition results in the degradation of mononegaviruses L protein, with exception of the rabies virus, for which the degradation of P protein was observed. Here, we demonstrated that Hsp90 inhibition does not affect the expression of rabies L and P proteins, but it inhibits binding of the P protein and L protein into functional viral polymerase. Rabies and the vesicular stomatitis virus, but not the measles virus, L proteins can be expressed independently of the presence of a P protein and in the presence of an Hsp90 inhibitor. Our results suggest that the interaction of L proteins with P proteins and Hsp90 in the process of polymerase maturation may be a process specific to particular viruses.<\/jats:p>","DOI":"10.3390\/ijms23136946","type":"journal-article","created":{"date-parts":[[2022,6,22]],"date-time":"2022-06-22T21:31:06Z","timestamp":1655933466000},"page":"6946","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Hsp90 Activity Is Necessary for the Maturation of Rabies Virus Polymerase"],"prefix":"10.3390","volume":"23","author":[{"given":"Iga","family":"Dalidowska","sequence":"first","affiliation":[{"name":"Mossakowski Medical Research Institute, Polish Academy of Sciences, 02-106 Warsaw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5781-115X","authenticated-orcid":false,"given":"Anna","family":"Orlowska","sequence":"additional","affiliation":[{"name":"Department of Virology, National Veterinary Research Institute, 24-100 Pu\u0142awy, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5957-846X","authenticated-orcid":false,"given":"Marcin","family":"Smreczak","sequence":"additional","affiliation":[{"name":"Department of Virology, National Veterinary Research Institute, 24-100 Pu\u0142awy, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1169-4517","authenticated-orcid":false,"given":"Pawel","family":"Bieganowski","sequence":"additional","affiliation":[{"name":"Mossakowski Medical Research Institute, Polish Academy of Sciences, 02-106 Warsaw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,6,22]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"698","DOI":"10.1016\/j.bbamcr.2011.11.007","article-title":"Broad action of Hsp90 as a host chaperone required for viral replication","volume":"1823","author":"Geller","year":"2012","journal-title":"Biochim. 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