{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T12:58:33Z","timestamp":1792155513015,"version":"4.4.0"},"reference-count":27,"publisher":"MDPI AG","issue":"13","license":[{"start":{"date-parts":[[2022,6,22]],"date-time":"2022-06-22T00:00:00Z","timestamp":1655856000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>With the rapidly increasing application of microwave technologies, the anxiety and speculation about microwave induced potential health hazards has been attracting more and more attention. In our daily life, people are exposed to complex environments with multi-frequency microwaves, especially L band and C band microwaves, which are commonly used in communications. In this study, we exposed rats to 1.5 GHz (L10), 4.3 GHz (C10) or multi-frequency (LC10) microwaves at an average power density of 10 mW\/cm2. Both single and multi-frequency microwaves induced slight pathological changes in the thymus and spleen. Additionally, the white blood cells (WBCs) and lymphocytes in peripheral blood were decreased at 6 h and 7 d after exposure, suggesting immune suppressive responses were induced. Among lymphocytes, the B lymphocytes were increased while the T lymphocytes were decreased at 7 d after exposure in the C10 and LC10 groups, but not in the L10 group. Moreover, multi-frequency microwaves regulated the B and T lymphocytes more strongly than the C band microwave. The results of transcriptomics and proteomics showed that both single and multi-frequency microwaves regulated numerous genes associated with immune regulation and cellular metabolism in peripheral blood and in the spleen. However, multi-frequency microwaves altered the expression of many more genes and proteins. Moreover, multi-frequency microwaves down-regulated T lymphocytes\u2019 development, differentiation and activation-associated genes, while they up-regulated B lymphocytes\u2019 activation-related genes. In conclusion, multi-frequency microwaves of 1.5 GHz and 4.3 GHz produced immune suppressive responses via regulating immune regulation and cellular metabolism-associated genes. Our findings provide meaningful information for exploring potential mechanisms underlying multi-frequency induced immune suppression.<\/jats:p>","DOI":"10.3390\/ijms23136949","type":"journal-article","created":{"date-parts":[[2022,6,22]],"date-time":"2022-06-22T21:31:06Z","timestamp":1655933466000},"page":"6949","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Immune Responses to Multi-Frequencies of 1.5 GHz and 4.3 GHz Microwave Exposure in Rats: Transcriptomic and Proteomic Analysis"],"prefix":"10.3390","volume":"23","author":[{"given":"Li","family":"Zhao","sequence":"first","affiliation":[{"name":"Beijing Institute of Radiation Medicine, Beijing 100850, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chuanfu","family":"Yao","sequence":"additional","affiliation":[{"name":"Beijing Institute of Radiation Medicine, Beijing 100850, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui","family":"Wang","sequence":"additional","affiliation":[{"name":"Beijing Institute of Radiation Medicine, Beijing 100850, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ji","family":"Dong","sequence":"additional","affiliation":[{"name":"Beijing Institute of Radiation Medicine, Beijing 100850, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Zhang","sequence":"additional","affiliation":[{"name":"Beijing Institute of Radiation Medicine, Beijing 100850, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinping","family":"Xu","sequence":"additional","affiliation":[{"name":"Beijing Institute of Radiation Medicine, Beijing 100850, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haoyu","family":"Wang","sequence":"additional","affiliation":[{"name":"Beijing Institute of Radiation Medicine, Beijing 100850, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Binwei","family":"Yao","sequence":"additional","affiliation":[{"name":"Beijing Institute of Radiation Medicine, Beijing 100850, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ke","family":"Ren","sequence":"additional","affiliation":[{"name":"Beijing Institute of Radiation Medicine, Beijing 100850, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liu","family":"Sun","sequence":"additional","affiliation":[{"name":"Beijing Institute of Radiation Medicine, Beijing 100850, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruiyun","family":"Peng","sequence":"additional","affiliation":[{"name":"Beijing Institute of Radiation Medicine, Beijing 100850, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,6,22]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"637","DOI":"10.1007\/s10661-018-7017-4","article-title":"Assessment of oil spills using Sentinel 1 C-band SAR and Landsat 8 multispectral sensors","volume":"190","author":"Arslan","year":"2018","journal-title":"Environ. Monit. Assess."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"673","DOI":"10.1016\/j.envres.2018.06.043","article-title":"Cancer epidemiology update, following the 2011 IARC evaluation of radiofrequency electromagnetic fields (Monograph 102)","volume":"167","author":"Miller","year":"2018","journal-title":"Environ. Res."},{"key":"ref_3","first-page":"315","article-title":"Cell phones, radio frequencies (RF) and health: IARC classification and epidemiological updates","volume":"42","author":"Oddone","year":"2020","journal-title":"G. Ital. Med. Lav. Ergon."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"643","DOI":"10.1016\/j.envpol.2018.07.019","article-title":"Thermal and non-thermal health effects of low intensity non-ionizing radiation: An international perspective","volume":"242","author":"Belpomme","year":"2018","journal-title":"Environ. Pollut."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"10061","DOI":"10.1038\/s41598-021-89348-4","article-title":"Effects of 1.5 and 4.3 GHz microwave radiation on cognitive function and hippocampal tissue structure in Wistar rats","volume":"11","author":"Zhu","year":"2021","journal-title":"Sci. Rep."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1159\/000495167","article-title":"Protective Role of NMDAR for Microwave-Induced Synaptic Plasticity Injuries in Primary Hippocampal Neurons","volume":"51","author":"Wang","year":"2018","journal-title":"Cell Physiol. Biochem."},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Zhang, X., Gao, Y., Dong, J., Wang, S., Yao, B., Zhang, J., Hu, S., Xu, X., Zuo, H., and Wang, L. (2014). The compound Chinese medicine \u201cKang Fu Ling\u201d protects against high power microwave-induced myocardial injury. PLoS ONE, 9.","DOI":"10.1371\/journal.pone.0101532"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1100","DOI":"10.3109\/09553002.2013.817701","article-title":"Impairment of long-term potentiation induction is essential for the disruption of spatial memory after microwave exposure","volume":"89","author":"Wang","year":"2013","journal-title":"Int. J. Radiat. Biol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"12348","DOI":"10.1038\/s41598-021-91622-4","article-title":"Acute effects of 2.856 GHz and 1.5 GHz microwaves on spatial memory abilities and CREB-related pathways","volume":"11","author":"Tan","year":"2021","journal-title":"Sci. Rep."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"10781","DOI":"10.1038\/s41598-017-11420-9","article-title":"Study on dose-dependent, frequency-dependent, and accumulative effects of 1.5\u2009 GHz and 2.856\u2009GHz microwave on cognitive functions in Wistar rats","volume":"7","author":"Tan","year":"2017","journal-title":"Sci. Rep."},{"key":"ref_11","first-page":"67","article-title":"Non-thermal biomarkers of exposure to radiofrequency\/microwave radiation","volume":"63","year":"2012","journal-title":"Arh. Hig. Rada Toksikol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"828","DOI":"10.1055\/s-0043-109010","article-title":"Thermal Ablation of Lung Tumors: Focus on Microwave Ablation","volume":"189","author":"Vogl","year":"2017","journal-title":"Rofo"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"3380","DOI":"10.1111\/1759-7714.14212","article-title":"CT-guided microwave ablation in patients with lung metastases from breast cancer","volume":"12","author":"Meng","year":"2021","journal-title":"Thorac. Cancer"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"639","DOI":"10.1080\/02656736.2021.1965224","article-title":"Volumetric analyses of ablation dimensions in microwave ablation for colorectal liver metastases","volume":"39","author":"Paolucci","year":"2022","journal-title":"Int. J. Hyperth."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1080\/13645706.2017.1286356","article-title":"Effects of microwave ablation on T-cell subsets and cytokines of patients with hepatocellular carcinoma","volume":"26","author":"Zhang","year":"2017","journal-title":"Minim. Invasive Ther. Allied Technol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.ijheh.2007.05.001","article-title":"Effects of low-level radio-frequency (3 kHz to 300 GHz) energy on human cardiovascular, reproductive, immune, and other systems: A review of the recent literature","volume":"211","author":"Jauchem","year":"2008","journal-title":"Int. J. Hyg. Environ. Health"},{"key":"ref_17","first-page":"17","article-title":"Autoimmune processes after long-term low-level exposure to electromagnetic fields (the results of an experiment). Part 3. The effect of the long-term non-thermal RF EMF exposure on complement-fixation antibodies against homologenous tissue","volume":"50","author":"Ivanov","year":"2010","journal-title":"Radiats Biol. Radioecol."},{"key":"ref_18","first-page":"323","article-title":"Microwave-induced apoptosis and cytotoxicity of NK cells through ERK1\/2 signaling","volume":"30","author":"Zhao","year":"2017","journal-title":"Biomed. Environ. Sci."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1104","DOI":"10.1016\/j.scitotenv.2019.06.494","article-title":"Synthetic aperture radar sensitivity to forest changes: A simulations-based study for the Romanian forests","volume":"689","author":"Tanase","year":"2019","journal-title":"Sci. Total Environ."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"110","DOI":"10.1007\/s10661-021-08902-9","article-title":"Spaceborne C-band SAR remote sensing-based flood mapping and runoff estimation for 2019 flood scenario in Rupnagar, Punjab, India","volume":"193","author":"Tripathi","year":"2021","journal-title":"Environ. Monit. Assess."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"115632","DOI":"10.1016\/j.envpol.2020.115632","article-title":"Effects of different mobile phone UMTS signals on DNA, apoptosis and oxidative stress in human lymphocytes","volume":"267","author":"Gulati","year":"2020","journal-title":"Environ. Pollut."},{"key":"ref_22","first-page":"154","article-title":"Exposure of 1800 MHz radiofrequency with SAR 1.6 W\/kg caused a significant reduction in CD4+ T cells and release of cytokines in-vitro","volume":"17","author":"Arthamin","year":"2020","journal-title":"Iran. J. Immunol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1093\/jrr\/rrz085","article-title":"Immunotropic effects in cultured human blood mononuclear cells exposed to a 900 MHz pulse-modulated microwave field","volume":"61","author":"Szymanski","year":"2020","journal-title":"J. Radiat. Res."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"224","DOI":"10.1016\/j.brainres.2008.08.013","article-title":"Effects of prenatal exposure to a 900 MHz electromagnetic field on the dentate gyrus of rats: A stereological and histopathological study","volume":"1238","author":"Odaci","year":"2008","journal-title":"Brain Res."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"334","DOI":"10.1007\/s00244-010-9477-6","article-title":"Combined exposure of peripubertal male rats to the endocrine-disrupting compound atrazine and power-frequency electromagnetic fields causes degranulation of cutaneous mast cells: A new toxic environmental hazard? Arch. Environ","volume":"59","author":"Rajkovic","year":"2010","journal-title":"Contam. Toxicol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"S187","DOI":"10.1002\/bem.10166","article-title":"Radiofrequency (RF) effects on blood cells, cardiac, endocrine, and immunological functions","volume":"24","author":"Black","year":"2003","journal-title":"Bioelectromagnetics"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1400","DOI":"10.1074\/mcp.M114.044305","article-title":"Extending the limits of quantitative proteome profiling with data-independent acquisition and application to acetaminophen-treated three-dimensional liver microtissues","volume":"14","author":"Bruderer","year":"2015","journal-title":"Mol. Cell. 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