{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,16]],"date-time":"2026-06-16T05:09:36Z","timestamp":1781586576847,"version":"3.54.5"},"reference-count":81,"publisher":"MDPI AG","issue":"13","license":[{"start":{"date-parts":[[2022,6,28]],"date-time":"2022-06-28T00:00:00Z","timestamp":1656374400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"the Science and technology development project of Jilin Province in China","award":["20210101030JC"],"award-info":[{"award-number":["20210101030JC"]}]},{"name":"the Science and technology development project of Jilin Province in China","award":["20210202069NC"],"award-info":[{"award-number":["20210202069NC"]}]},{"name":"Science and technology development project of Jilin Province in China","award":["20210101030JC"],"award-info":[{"award-number":["20210101030JC"]}]},{"name":"Science and technology development project of Jilin Province in China","award":["20210202069NC"],"award-info":[{"award-number":["20210202069NC"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Knowing the molecular mechanism of male sterility in alfalfa is important to utilize the heterosis more effectively. However, the molecular mechanisms of male sterility in alfalfa are still unclear. In this study, the bulked segregant analysis (BSA) and bulked segregant RNA-seq (BSR) were performed with F2 separation progeny to study the molecular mechanism of male sterility in alfalfa. The BSA-seq analysis was located in a candidate region on chromosome 5 containing 626 candidate genes which were associated with male sterility in alfalfa, while the BSR-seq analysis filtered seven candidate DEGs related to male sterility, and these candidate genes including EF-Tu, \u03b2-GAL, CESA, PHGDH, and JMT. The conjunctive analyses of BSR and BSA methods revealed that the genes of Ms\u03b2-GAL and MsJMT are the common detected candidate genes involved in male sterility in alfalfa. Our research provides a theory basis for further study of the molecular mechanism of male sterility in alfalfa and significant information for the genetic breeding of Medicago sativa.<\/jats:p>","DOI":"10.3390\/ijms23137172","type":"journal-article","created":{"date-parts":[[2022,6,28]],"date-time":"2022-06-28T23:59:22Z","timestamp":1656460762000},"page":"7172","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Conjunctive Analyses of BSA-Seq and BSR-Seq Unveil the Ms\u03b2-GAL and MsJMT as Key Candidate Genes for Cytoplasmic Male Sterility in Alfalfa (Medicago sativa L.)"],"prefix":"10.3390","volume":"23","author":[{"given":"Le","family":"Zhou","sequence":"first","affiliation":[{"name":"College of Forestry and Grassland, Jilin Agricultural University, Changchun 130118, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yingzhe","family":"Wang","sequence":"additional","affiliation":[{"name":"Institute of Agricultural Biotechnology, Jilin Academy of Agricultural Sciences (JAAS), Changchun 130119, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1241-6797","authenticated-orcid":false,"given":"Xiaobo","family":"Xu","sequence":"additional","affiliation":[{"name":"College of Forestry and Grassland, Jilin Agricultural University, Changchun 130118, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dong","family":"Yan","sequence":"additional","affiliation":[{"name":"College of Forestry and Grassland, Jilin Agricultural University, Changchun 130118, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weijie","family":"Yu","sequence":"additional","affiliation":[{"name":"College of Forestry and Grassland, Jilin Agricultural University, Changchun 130118, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yifan","family":"Miao","sequence":"additional","affiliation":[{"name":"College of Forestry and Grassland, Jilin Agricultural University, Changchun 130118, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bo","family":"Xu","sequence":"additional","affiliation":[{"name":"College of Forestry and Grassland, Jilin Agricultural University, Changchun 130118, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,6,28]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1016\/j.gene.2017.10.022","article-title":"Analysis of the meiotic transcriptome reveals the genes related to the regulation of pollen abortion in cytoplasmic male-sterile pepper (Capsicum annuum L.)","volume":"641","author":"Qiu","year":"2018","journal-title":"Gene"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1016\/S1360-1385(98)01235-7","article-title":"The molecular basis of cytoplasmic male sterility and fertility restoration","volume":"3","author":"Schnable","year":"1998","journal-title":"Trends Plant Sci."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"224","DOI":"10.1016\/j.plaphy.2016.04.027","article-title":"Transcriptome de novo assembly and analysis of differentially expressed genes related to cytoplasmic male sterility in cabbage","volume":"105","author":"Wang","year":"2016","journal-title":"Plant Physiol. 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