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Ribosomes are the machinery responsible for protein synthesis. However, there remains little information on whether current psychotropic drugs affect ribosomes and contribute to their therapeutic effects. We treated human neuronal-like (NT2-N) cells with amisulpride (10 \u00b5M), aripiprazole (0.1 \u00b5M), clozapine (10 \u00b5M), lamotrigine (50 \u00b5M), lithium (2.5 mM), quetiapine (50 \u00b5M), risperidone (0.1 \u00b5M), valproate (0.5 mM) or vehicle control for 24 h. Transcriptomic and gene set enrichment analysis (GSEA) identified that the ribosomal pathway was altered by these drugs. We found that three of the eight drugs tested significantly decreased ribosomal gene expression, whilst one increased it. Most changes were observed in the components of cytosolic ribosomes and not mitochondrial ribosomes. Protein synthesis assays revealed that aripiprazole, clozapine and lithium all decreased protein synthesis. Several currently prescribed psychotropic drugs seem to impact ribosomal gene expression and protein synthesis. This suggests the possibility of using protein synthesis inhibitors as novel therapeutic agents for neuropsychiatric disorders.<\/jats:p>","DOI":"10.3390\/ijms23137180","type":"journal-article","created":{"date-parts":[[2022,6,28]],"date-time":"2022-06-28T23:59:22Z","timestamp":1656460762000},"page":"7180","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Effects of Psychotropic Drugs on Ribosomal Genes and Protein Synthesis"],"prefix":"10.3390","volume":"23","author":[{"given":"Zoe S. J.","family":"Liu","sequence":"first","affiliation":[{"name":"The Institute for Mental and Physical Health and Clinical Translation (IMPACT), School of Medicine, Deakin University, Geelong 3220, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9301-5911","authenticated-orcid":false,"given":"Trang T. T.","family":"Truong","sequence":"additional","affiliation":[{"name":"The Institute for Mental and Physical Health and Clinical Translation (IMPACT), School of Medicine, Deakin University, Geelong 3220, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0794-6363","authenticated-orcid":false,"given":"Chiara C.","family":"Bortolasci","sequence":"additional","affiliation":[{"name":"The Institute for Mental and Physical Health and Clinical Translation (IMPACT), School of Medicine, Deakin University, Geelong 3220, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Briana","family":"Spolding","sequence":"additional","affiliation":[{"name":"The Institute for Mental and Physical Health and Clinical Translation (IMPACT), School of Medicine, Deakin University, Geelong 3220, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8825-734X","authenticated-orcid":false,"given":"Bruna","family":"Panizzutti","sequence":"additional","affiliation":[{"name":"The Institute for Mental and Physical Health and Clinical Translation (IMPACT), School of Medicine, Deakin University, Geelong 3220, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Courtney","family":"Swinton","sequence":"additional","affiliation":[{"name":"The Institute for Mental and Physical Health and Clinical Translation (IMPACT), School of Medicine, Deakin University, Geelong 3220, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1299-4300","authenticated-orcid":false,"given":"Jee Hyun","family":"Kim","sequence":"additional","affiliation":[{"name":"The Institute for Mental and Physical Health and Clinical Translation (IMPACT), School of Medicine, Deakin University, Geelong 3220, Australia"},{"name":"Florey Institute of Neuroscience and Mental Health, Parkville 3010, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Srisaiyini","family":"Kidnapillai","sequence":"additional","affiliation":[{"name":"The Institute for Mental and Physical Health and Clinical Translation (IMPACT), School of Medicine, Deakin University, Geelong 3220, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark F.","family":"Richardson","sequence":"additional","affiliation":[{"name":"Genomics Centre, School of Life and Environmental Sciences, Deakin University, Burwood 3125, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"Gray","sequence":"additional","affiliation":[{"name":"The Institute for Mental and Physical Health and Clinical Translation (IMPACT), School of Medicine, Deakin University, Geelong 3220, Australia"},{"name":"Florey Institute of Neuroscience and Mental Health, Parkville 3010, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2776-3935","authenticated-orcid":false,"given":"Olivia M.","family":"Dean","sequence":"additional","affiliation":[{"name":"The Institute for Mental and Physical Health and Clinical Translation (IMPACT), School of Medicine, Deakin University, Geelong 3220, Australia"},{"name":"Florey Institute of Neuroscience and Mental Health, Parkville 3010, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sean L.","family":"McGee","sequence":"additional","affiliation":[{"name":"The Institute for Mental and Physical Health and Clinical Translation (IMPACT), School of Medicine, Deakin University, Geelong 3220, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Berk","sequence":"additional","affiliation":[{"name":"The Institute for Mental and Physical Health and Clinical Translation (IMPACT), School of Medicine, Deakin University, Geelong 3220, Australia"},{"name":"Florey Institute of Neuroscience and Mental Health, Parkville 3010, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6758-4763","authenticated-orcid":false,"given":"Ken","family":"Walder","sequence":"additional","affiliation":[{"name":"The Institute for Mental and Physical Health and Clinical Translation (IMPACT), School of Medicine, Deakin University, Geelong 3220, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,6,28]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1097\/YPG.0000000000000076","article-title":"Active ribosomal genes, translational homeostasis and oxidative stress in the pathogenesis of schizophrenia and autism","volume":"25","author":"Porokhovnik","year":"2015","journal-title":"Psychiatr. Genet."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"305","DOI":"10.1016\/j.schres.2018.01.001","article-title":"Abundance of ribosomal RNA gene copies in the genomes of schizophrenia patients","volume":"197","author":"Chestkov","year":"2018","journal-title":"Schizophr. Res."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"202","DOI":"10.1016\/j.jpsychires.2018.08.010","article-title":"Integrated profiling of phenotype and blood transcriptome for stress vulnerability and depression","volume":"104","author":"Hori","year":"2018","journal-title":"J. Psychiatr. Res."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1016\/j.ebiom.2018.07.022","article-title":"The Long Noncoding RNA Landscape in Amygdala Tissues from Schizophrenia Patients","volume":"34","author":"Tian","year":"2018","journal-title":"EBioMedicine"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"72","DOI":"10.1038\/nature12630","article-title":"SHANK3 overexpression causes manic-like behaviour with unique pharmacogenetic properties","volume":"503","author":"Han","year":"2013","journal-title":"Nature"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"7863","DOI":"10.1073\/pnas.0906232107","article-title":"De novo mutations in the gene encoding the synaptic scaffolding protein SHANK3 in patients ascertained for schizophrenia","volume":"107","author":"Gauthier","year":"2010","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1038\/s12276-018-0187-x","article-title":"Excitatory and inhibitory synaptic dysfunction in mania: An emerging hypothesis from animal model studies","volume":"50","author":"Lee","year":"2018","journal-title":"Exp. Mol. Med."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"250","DOI":"10.3389\/fnmol.2018.00250","article-title":"Integrative Brain Transcriptome Analysis Reveals Region-Specific and Broad Molecular Changes in Shank3-Overexpressing Mice","volume":"11","author":"Jin","year":"2018","journal-title":"Front. Mol. Neurosci."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"a011536","DOI":"10.1101\/cshperspect.a011536","article-title":"The structure and function of the eukaryotic ribosome","volume":"4","author":"Wilson","year":"2012","journal-title":"Cold Spring Harb. Perspect. Biol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"514","DOI":"10.1038\/35080045","article-title":"The function and synthesis of ribosomes","volume":"2","author":"Lafontaine","year":"2001","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"311","DOI":"10.1016\/j.ijdevneu.2010.08.007","article-title":"Mitochondrial dysfunction and pathology in bipolar disorder and schizophrenia","volume":"29","author":"Clay","year":"2011","journal-title":"Int. J. Dev. Neurosci."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"567","DOI":"10.2147\/NDT.S44282","article-title":"Mitochondrial dysfunction, oxidative stress, and major depressive disorder","volume":"9","author":"Tobe","year":"2013","journal-title":"Neuropsychiatr. Dis. Treat."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1146\/annurev-biochem-060815-014343","article-title":"Structure and function of the mitochondrial ribosome","volume":"85","author":"Greber","year":"2016","journal-title":"Annu. Rev. Biochem."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1177\/1073858419853236","article-title":"Protein Translation and Psychiatric Disorders","volume":"26","author":"Laguesse","year":"2020","journal-title":"Neuroscientist"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1038\/nrn.2018.16","article-title":"Dendritic structural plasticity and neuropsychiatric disease","volume":"19","author":"Forrest","year":"2018","journal-title":"Nat. Rev. Neurosci."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1453","DOI":"10.1017\/S1461145714000443","article-title":"Impaired endoplasmic reticulum stress response in bipolar disorder: Cellular evidence of illness progression","volume":"17","author":"Pfaffenseller","year":"2014","journal-title":"Int. J. Neuropsychopharmacol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"449","DOI":"10.1016\/j.mcn.2010.08.004","article-title":"Identification of eukaryotic elongation factor-2 as a novel cellular target of lithium and glycogen synthase kinase-3","volume":"45","author":"Karyo","year":"2010","journal-title":"Mol. Cell. Neurosci."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"6127","DOI":"10.1038\/s41467-021-26365-x","article-title":"Neuronal ribosomes exhibit dynamic and context-dependent exchange of ribosomal proteins","volume":"12","author":"Fusco","year":"2021","journal-title":"Nat. Commun."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"e663","DOI":"10.1038\/tp.2015.119","article-title":"Reduced protein synthesis in schizophrenia patient-derived olfactory cells","volume":"5","author":"English","year":"2015","journal-title":"Transl. Psychiatry"},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Heaney, C.F., and Raab-Graham, K.F. (2018). Dysregulated Protein Synthesis in Major Depressive Disorder. The Oxford Handbook of Neuronal Protein Synthesis, Oxford University Press.","DOI":"10.1093\/oxfordhb\/9780190686307.013.3"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"594","DOI":"10.1016\/j.biopsych.2017.03.018","article-title":"Transcriptome alterations in prefrontal pyramidal cells distinguish schizophrenia from bipolar and major depressive disorders","volume":"82","author":"Arion","year":"2017","journal-title":"Biol. Psychiatry"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"258","DOI":"10.1002\/ajmg.b.32722","article-title":"Genetic association and functional characterization of MCPH1 gene variation in bipolar disorder and schizophrenia","volume":"180","author":"Sharp","year":"2019","journal-title":"Am. J. Med. Genet. B Neuropsychiatr. Genet."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"110408","DOI":"10.1016\/j.pnpbp.2021.110408","article-title":"Dose-dependent transcriptional effects of lithium and adverse effect burden in a psychiatric cohort","volume":"112","author":"Krull","year":"2022","journal-title":"Prog. Neuro-Psychopharmacol. Biol. Psychiatry"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"947","DOI":"10.1038\/s41386-019-0556-8","article-title":"Exploring lithium\u2019s transcriptional mechanisms of action in bipolar disorder: A multi-step study","volume":"45","author":"Akkouh","year":"2020","journal-title":"Neuropsychopharmacology"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"e1025185","DOI":"10.1080\/21675511.2015.1025185","article-title":"Ribosomopathies: Global process, tissue specific defects","volume":"3","author":"Yelick","year":"2015","journal-title":"Rare Dis."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"506","DOI":"10.1016\/j.ajhg.2017.01.034","article-title":"A Ribosomopathy Reveals Decoding Defective Ribosomes Driving Human Dysmorphism","volume":"100","author":"Paolini","year":"2017","journal-title":"Am. J. Hum. Genet."},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Chakraborty, A., Uechi, T., Higa, S., Torihara, H., and Kenmochi, N. (2009). Loss of ribosomal protein L11 affects zebrafish embryonic development through a p53-dependent apoptotic response. PLoS ONE, 4.","DOI":"10.1371\/journal.pone.0004152"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"6","DOI":"10.1186\/1756-9966-28-6","article-title":"RPS2: A novel therapeutic target in prostate cancer","volume":"28","author":"Wang","year":"2009","journal-title":"J. Exp. Clin. Cancer Res."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"283","DOI":"10.1002\/jbmr.4177","article-title":"Novel RPL13 Variants and Variable Clinical Expressivity in a Human Ribosomopathy With Spondyloepimetaphyseal Dysplasia","volume":"36","author":"Costantini","year":"2021","journal-title":"J. Bone Miner. Res."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"616402","DOI":"10.3389\/fimmu.2021.616402","article-title":"Ribosomal Protein L13 Participates in Innate Immune Response Induced by Foot-and-Mouth Disease Virus","volume":"12","author":"Guan","year":"2021","journal-title":"Front. Immunol."},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Wei, X., Zhang, Y., Fu, Z., and Zhang, L. (2013). The association between polymorphisms in the MRPL4 and TNF-\u03b1 genes and susceptibility to allergic rhinitis. PLoS ONE, 8.","DOI":"10.1371\/journal.pone.0057981"},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Andiappan, A.K., Wang, D.Y., Anantharaman, R., Parate, P.N., Suri, B.K., Low, H.Q., Li, Y., Zhao, W., Castagnoli, P., and Liu, J. (2011). Genome-wide association study for atopy and allergic rhinitis in a Singapore Chinese population. PLoS ONE, 6.","DOI":"10.1371\/journal.pone.0019719"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"685","DOI":"10.1016\/j.ajhg.2018.02.012","article-title":"Bi-allelic Mutations in the Mitochondrial Ribosomal Protein MRPS2 Cause Sensorineural Hearing Loss, Hypoglycemia, and Multiple OXPHOS Complex Deficiencies","volume":"102","author":"Gardeitchik","year":"2018","journal-title":"Am. J. Hum. Genet."},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Liu, C., Zhou, W., Liu, Q., and Peng, Z. (2022). Hypoglycemia with lactic acidosis caused by a new MRPS2 gene mutation in a Chinese girl: A case report. BMC Endocr. Disord., 22.","DOI":"10.1186\/s12902-021-00924-1"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1304","DOI":"10.1016\/j.bbadis.2013.04.014","article-title":"Mutations in mitochondrial ribosomal protein MRPL12 leads to growth retardation, neurological deterioration and mitochondrial translation deficiency","volume":"1832","author":"Serre","year":"2013","journal-title":"Biochim. Biophys. Acta (BBA)-Mol. Basis Dis."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1016\/bs.apha.2017.09.009","article-title":"Unique Effects of Clozapine: A Pharmacological Perspective","volume":"82","author":"Khokhar","year":"2018","journal-title":"Adv. Pharmacol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1186\/gb-2003-4-9-117","article-title":"Comparing protein abundance and mRNA expression levels on a genomic scale","volume":"4","author":"Greenbaum","year":"2003","journal-title":"Genome Biol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1080\/07388550701334212","article-title":"Integrative analysis of transcriptomic and proteomic data: Challenges, solutions and applications","volume":"27","author":"Nie","year":"2007","journal-title":"Crit. Rev. Biotechnol."},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Gry, M., Rimini, R., Str\u00f6mberg, S., Asplund, A., Pont\u00e9n, F., Uhl\u00e9n, M., and Nilsson, P. (2009). Correlations between RNA and protein expression profiles in 23 human cell lines. BMC Genom., 10.","DOI":"10.1186\/1471-2164-10-365"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"261","DOI":"10.1093\/bfgp\/ell019","article-title":"Data merging for integrated microarray and proteomic analysis","volume":"5","author":"Waters","year":"2006","journal-title":"Brief. Funct. Genom. Proteomic"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"883","DOI":"10.15252\/msb.20167144","article-title":"Gene-specific correlation of RNA and protein levels in human cells and tissues","volume":"12","author":"Edfors","year":"2016","journal-title":"Mol. Syst. Biol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"410","DOI":"10.1089\/dna.2005.24.410","article-title":"Correlation of protein and gene expression profiles of inflammatory proteins after endotoxin challenge in human subjects","volume":"24","author":"Prabhakar","year":"2005","journal-title":"DNA Cell Biol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"325","DOI":"10.1097\/01.pra.0000388627.36781.6a","article-title":"Clozapine treatment causes oxidation of proteins involved in energy metabolism in lymphoblastoid cells: A possible mechanism for antipsychotic-induced metabolic alterations","volume":"16","author":"Baig","year":"2010","journal-title":"J. Psychiatr. Pract."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"502","DOI":"10.1016\/j.ijbiomac.2021.04.155","article-title":"Antipsychotic clozapine binding to alpha-2-macroglobulin protects interacting partners against oxidation and preserves the anti-proteinase activity of the protein","volume":"183","year":"2021","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"1351","DOI":"10.1515\/BC.2006.169","article-title":"Protein oxidation and proteolysis","volume":"387","author":"Bader","year":"2006","journal-title":"Biol. Chem."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"630","DOI":"10.1016\/j.euroneuro.2013.10.014","article-title":"Effects of lithium and aripiprazole on brain stimulation reward and neuroplasticity markers in the limbic forebrain","volume":"24","author":"Mavrikaki","year":"2014","journal-title":"Eur. Neuropsychopharmacol."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"110380","DOI":"10.18632\/oncotarget.22777","article-title":"Neuroprotection by aripiprazole against \u03b2-amyloid-induced toxicity by P-CK2\u03b1 activation via inhibition of GSK-3\u03b2","volume":"8","author":"Park","year":"2017","journal-title":"Oncotarget"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"E1204","DOI":"10.1073\/pnas.1110195108","article-title":"Glycogen synthase kinase (GSK)-3 promotes p70 ribosomal protein S6 kinase (p70S6K) activity and cell proliferation","volume":"108","author":"Shin","year":"2011","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"186","DOI":"10.3389\/fphar.2019.00186","article-title":"Biochemical Pathways Triggered by Antipsychotics in Human Oligodendrocytes: Potential of Discovering New Treatment Targets","volume":"10","author":"Cassoli","year":"2019","journal-title":"Front. Pharmacol."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"362","DOI":"10.1152\/physiol.00024.2006","article-title":"The mTOR pathway in the control of protein synthesis","volume":"21","author":"Wang","year":"2006","journal-title":"Physiology"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"467","DOI":"10.1073\/pnas.012605299","article-title":"A rapamycin-sensitive signaling pathway contributes to long-term synaptic plasticity in the hippocampus","volume":"99","author":"Tang","year":"2002","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_52","doi-asserted-by":"crossref","unstructured":"Ma, T., Hoeffer, C.A., Capetillo-Zarate, E., Yu, F., Wong, H., Lin, M.T., Tampellini, D., Klann, E., Blitzer, R.D., and Gouras, G.K. (2010). Dysregulation of the mTOR pathway mediates impairment of synaptic plasticity in a mouse model of Alzheimer\u2019s disease. PLoS ONE, 5.","DOI":"10.1371\/journal.pone.0012845"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"e8098","DOI":"10.1590\/1414-431x20188098","article-title":"Effect of risperidone on proliferation and apoptosis of MC3T3-E1 cells","volume":"52","author":"Zheng","year":"2019","journal-title":"Braz. J. Med. Biol. Res."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"1802","DOI":"10.1523\/JNEUROSCI.12-05-01802.1992","article-title":"Pure, postmitotic, polarized human neurons derived from NTera 2 cells provide a system for expressing exogenous proteins in terminally differentiated neurons","volume":"12","author":"Pleasure","year":"1992","journal-title":"J. Neurosci."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1016\/j.neulet.2004.09.070","article-title":"Expression of neuronal markers during NTera2\/cloneD1 differentiation by cell aggregation method","volume":"373","author":"Megiorni","year":"2005","journal-title":"Neurosci. Lett."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"582","DOI":"10.1093\/ijnp\/pyy014","article-title":"Mechanisms underpinning the polypharmacy effects of medications in psychiatry","volume":"21","author":"Bortolasci","year":"2018","journal-title":"Int. J. Neuropsychopharmacol."},{"key":"ref_57","doi-asserted-by":"crossref","unstructured":"Bortolasci, C.C., Spolding, B., Kidnapillai, S., Connor, T., Truong, T.T., Liu, Z.S., Panizzutti, B., Richardson, M.F., Gray, L., and Berk, M. (2020). Transcriptional Effects of Psychoactive Drugs on Genes Involved in Neurogenesis. Int. J. Mol. Sci., 21.","DOI":"10.3390\/ijms21218333"},{"key":"ref_58","doi-asserted-by":"crossref","unstructured":"Panizzutti, B., Bortolasci, C.C., Spolding, B., Kidnapillai, S., Connor, T., Richardson, M.F., Truong, T.T.T., Liu, Z.S.J., Morris, G., and Gray, L. (2021). Transcriptional Modulation of the Hippo Signaling Pathway by Drugs Used to Treat Bipolar Disorder and Schizophrenia. Int. J. Mol. Sci., 22.","DOI":"10.3390\/ijms22137164"},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"2114","DOI":"10.1093\/bioinformatics\/btu170","article-title":"Trimmomatic: A flexible trimmer for Illumina sequence data","volume":"30","author":"Bolger","year":"2014","journal-title":"Bioinformatics"},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1093\/bioinformatics\/bts635","article-title":"STAR: Ultrafast universal RNA-seq aligner","volume":"29","author":"Dobin","year":"2013","journal-title":"Bioinformatics"},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1093\/bioinformatics\/btp616","article-title":"edgeR: A Bioconductor package for differential expression analysis of digital gene expression data","volume":"26","author":"Robinson","year":"2010","journal-title":"Bioinformatics"},{"key":"ref_62","unstructured":"R Core Team (2013). R: A Language and Environment for Statistical Computing, R Foundation for Statistical Computing."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"284","DOI":"10.1089\/omi.2011.0118","article-title":"clusterProfiler: An R package for comparing biological themes among gene clusters","volume":"16","author":"Yu","year":"2012","journal-title":"Omics A J. Integr. Biol."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"15545","DOI":"10.1073\/pnas.0506580102","article-title":"Gene set enrichment analysis: A knowledge-based approach for interpreting genome-wide expression profiles","volume":"102","author":"Subramanian","year":"2005","journal-title":"Proc. Natl. Acad. Sci. USA"}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/23\/13\/7180\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T23:39:46Z","timestamp":1760139586000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/23\/13\/7180"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,6,28]]},"references-count":64,"journal-issue":{"issue":"13","published-online":{"date-parts":[[2022,7]]}},"alternative-id":["ijms23137180"],"URL":"https:\/\/doi.org\/10.3390\/ijms23137180","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,6,28]]}}}