{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T19:09:10Z","timestamp":1787166550128,"version":"build-2736575974"},"reference-count":39,"publisher":"MDPI AG","issue":"13","license":[{"start":{"date-parts":[[2022,6,29]],"date-time":"2022-06-29T00:00:00Z","timestamp":1656460800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"European Union\u2019s Horizon 2020 research","award":["860197"],"award-info":[{"award-number":["860197"]}]},{"name":"innovation program Marie Curie ITN \u2018MIRIADE\u2019","award":["860197"],"award-info":[{"award-number":["860197"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Neurofilament light chain (Nf-L) is a well-known biomarker for axonal damage; however, the corresponding circulating Nf-L analyte in cerebrospinal fluid (CSF) is poorly characterized. We therefore isolated new monoclonal antibodies against synthetic peptides, and these monoclonals were characterized for their specificity on brain-specific intermediate filament proteins. Two highly specific antibodies, ADx206 and ADx209, were analytically validated for CSF applications according to well-established criteria. Interestingly, using three different sources of purified Nf-L proteins, a significant impact on interpolated concentrations was observed. With a lower limit of analytical sensitivity of 100 pg\/mL using bovine Nf-L as the calibrator, we were able to quantify the Nf-L analyte in each sample, and these Nf-L concentrations were highly correlated to the Uman diagnostics assay (Spearman rho = 0.97, p &lt; 0.001). In the clinical diagnostic groups, the new Nf-L ELISA could discriminate patients with Alzheimer\u2019s disease (AD, n = 20) from those with frontotemporal lobe dementia (FTD, n = 20) and control samples with subjective cognitive decline (SCD, n = 20). Henceforth, this novel Nf-L ELISA with well-defined specificity and epitopes can be used to enhance our understanding of harmonizing the use of Nf-L as a clinically relevant marker for neurodegeneration in CSF.<\/jats:p>","DOI":"10.3390\/ijms23137221","type":"journal-article","created":{"date-parts":[[2022,6,29]],"date-time":"2022-06-29T20:47:56Z","timestamp":1656535676000},"page":"7221","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["A Novel Neurofilament Light Chain ELISA Validated in Patients with Alzheimer\u2019s Disease, Frontotemporal Dementia, and Subjective Cognitive Decline, and the Evaluation of Candidate Proteins for Immunoassay Calibration"],"prefix":"10.3390","volume":"23","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2147-3961","authenticated-orcid":false,"given":"Shreyasee","family":"Das","sequence":"first","affiliation":[{"name":"ADx NeuroSciences NV, Technologiepark-Zwijnaarde 94, 9052 Gent, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nele","family":"Dewit","sequence":"additional","affiliation":[{"name":"ADx NeuroSciences NV, Technologiepark-Zwijnaarde 94, 9052 Gent, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dirk","family":"Jacobs","sequence":"additional","affiliation":[{"name":"ADx NeuroSciences NV, Technologiepark-Zwijnaarde 94, 9052 Gent, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yolande A. L.","family":"Pijnenburg","sequence":"additional","affiliation":[{"name":"Department of Clinical Chemistry, Neurochemistry Laboratory, Amsterdam University Medical Centres, Vrije Universiteit, Amsterdam Neuroscience Neurodegeneration, De Boelelaan 1117, 1081 HV Amsterdam, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3680-8437","authenticated-orcid":false,"given":"Sjors G. J. G.","family":"In \u2018t Veld","sequence":"additional","affiliation":[{"name":"Department of Clinical Chemistry, Neurochemistry Laboratory, Amsterdam University Medical Centres, Vrije Universiteit, Amsterdam Neuroscience Neurodegeneration, De Boelelaan 1117, 1081 HV Amsterdam, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Salom\u00e9","family":"Coppens","sequence":"additional","affiliation":[{"name":"National Measurement Laboratory, LGC Limited, Teddington TW11 0LY, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Milena","family":"Quaglia","sequence":"additional","affiliation":[{"name":"National Measurement Laboratory, LGC Limited, Teddington TW11 0LY, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7313-0629","authenticated-orcid":false,"given":"Christophe","family":"Hirtz","sequence":"additional","affiliation":[{"name":"IRMB-PPC, INM, CHU Montpellier, INSERM CNRS, 34295 Montpellier, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4061-0837","authenticated-orcid":false,"given":"Charlotte E.","family":"Teunissen","sequence":"additional","affiliation":[{"name":"Department of Clinical Chemistry, Neurochemistry Laboratory, Amsterdam University Medical Centres, Vrije Universiteit, Amsterdam Neuroscience Neurodegeneration, De Boelelaan 1117, 1081 HV Amsterdam, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9466-5366","authenticated-orcid":false,"given":"Eugeen","family":"Vanmechelen","sequence":"additional","affiliation":[{"name":"ADx NeuroSciences NV, Technologiepark-Zwijnaarde 94, 9052 Gent, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,6,29]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Ching, G.Y., and Liem, R.K.H. 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