{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T21:37:08Z","timestamp":1792186628424,"version":"4.4.0"},"reference-count":85,"publisher":"MDPI AG","issue":"13","license":[{"start":{"date-parts":[[2022,6,30]],"date-time":"2022-06-30T00:00:00Z","timestamp":1656547200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"C\u00e1mara de Diputados, INMUJERES Proyecto de g\u00e9nero","award":["NC-093380.1"],"award-info":[{"award-number":["NC-093380.1"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Stress susceptibility could play a role in developing premenstrual anxiety due to abnormalities in the hypothalamus\u2013pituitary\u2013adrenal (HPA) axis and impairments in the GABAA receptors\u2019 benzodiazepine (BDZ) site. Hence, we studied the stress-vulnerable Wistar Kyoto rat strain (WKY) to evaluate progesterone withdrawal (PW) effects on anxiety, HPA axis response, and to explore indicators of GABAA functionality in the BDZ site. For five days, ovariectomized WKY rats were administered 2.0 mg\/kg of progesterone. Twenty-four hours after the last administration, rats were tested in the anxiety-like burying behavior test (BBT) or elevated plus maze test (EPM), and corticosterone was determined. [3H]Flunitrazepam binding autoradiography served as the BDZ binding site index of the GABAA receptor in amygdala nuclei and hippocampus\u2019s dentate gyrus (DG). Finally, different doses of diazepam in PW-WKY rats were tested in the BBT. PW induced anxiety-like behaviors in both BBT and EPM compared with No-PW rats. PW increased corticosterone, but was blunted when combined with PW and BBT. PW increased [3H]Flunitrazepam binding in the DG and central amygdala compared with No-PW rats. Diazepam at a low dose induced an anxiogenic-like response in PW rats, suggesting a paradoxical response to benzodiazepines. Overall, PW induced anxiety-like behavior, a blunted HPA axis response, and higher GABAAR\/BZD binding site sensitivity in a stress-vulnerable rat strain. These findings demonstrate the role of stress-susceptibility in GABAAR functionality in a preclinical approximation of PMDD.<\/jats:p>","DOI":"10.3390\/ijms23137259","type":"journal-article","created":{"date-parts":[[2022,6,30]],"date-time":"2022-06-30T01:38:48Z","timestamp":1656553128000},"page":"7259","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Anxiety-like Behavior and GABAAR\/BDZ Binding Site Response to Progesterone Withdrawal in a Stress-Vulnerable Strain, the Wistar Kyoto Rats"],"prefix":"10.3390","volume":"23","author":[{"given":"Dannia","family":"Islas-Preciado","sequence":"first","affiliation":[{"name":"Lab de Neuropsicofarmacolog\u00eda, Direcci\u00f3n de Neurociencias, Instituto Nacional de Psiquiatr\u00eda Ram\u00f3n de la Fuente Mu\u00f1iz, Ciudad de M\u00e9xico 14370, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabriela","family":"Ugalde-Fuentes","sequence":"additional","affiliation":[{"name":"Lab de Neuropsicofarmacolog\u00eda, Direcci\u00f3n de Neurociencias, Instituto Nacional de Psiquiatr\u00eda Ram\u00f3n de la Fuente Mu\u00f1iz, Ciudad de M\u00e9xico 14370, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4569-3643","authenticated-orcid":false,"given":"Isabel","family":"Sollozo-Dupont","sequence":"additional","affiliation":[{"name":"Lab de Neuropsicofarmacolog\u00eda, Direcci\u00f3n de Neurociencias, Instituto Nacional de Psiquiatr\u00eda Ram\u00f3n de la Fuente Mu\u00f1iz, Ciudad de M\u00e9xico 14370, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4399-1508","authenticated-orcid":false,"given":"Mar\u00eda Eva","family":"Gonz\u00e1lez Trujano","sequence":"additional","affiliation":[{"name":"Lab de Neurofarmacolog\u00eda de Productos Naturales, Direcci\u00f3n de Neurociencias, Instituto Nacional de Psiquiatr\u00eda Ram\u00f3n de la Fuente Mu\u00f1iz, Ciudad de M\u00e9xico 14370, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nancy","family":"Cervantes-Anaya","sequence":"additional","affiliation":[{"name":"Lab de Neuropsicofarmacolog\u00eda, Direcci\u00f3n de Neurociencias, Instituto Nacional de Psiquiatr\u00eda Ram\u00f3n de la Fuente Mu\u00f1iz, Ciudad de M\u00e9xico 14370, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6634-7494","authenticated-orcid":false,"given":"Erika","family":"Estrada-Camarena","sequence":"additional","affiliation":[{"name":"Lab de Neuropsicofarmacolog\u00eda, Direcci\u00f3n de Neurociencias, Instituto Nacional de Psiquiatr\u00eda Ram\u00f3n de la Fuente Mu\u00f1iz, Ciudad de M\u00e9xico 14370, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9562-7193","authenticated-orcid":false,"given":"Carolina","family":"L\u00f3pez-Rubalcava","sequence":"additional","affiliation":[{"name":"Departamento de Farmacobiolog\u00eda, Centro de Investigaci\u00f3n y de Estudios Avanzados IPN (Cinvestav-IPN), Ciudad de M\u00e9xico 07360, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,6,30]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"88","DOI":"10.1016\/j.pneurobio.2013.07.005","article-title":"Allopregnanolone and Mood Disorders","volume":"113","author":"Bixo","year":"2014","journal-title":"Prog. 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