{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T04:12:10Z","timestamp":1762056730465,"version":"build-2065373602"},"reference-count":66,"publisher":"MDPI AG","issue":"13","license":[{"start":{"date-parts":[[2022,7,2]],"date-time":"2022-07-02T00:00:00Z","timestamp":1656720000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"European Union\u2019s Horizon 2020 Research and Innovation Program","award":["812915"],"award-info":[{"award-number":["812915"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>A licensed Chlamydia trachomatis (Ct) vaccine is not yet available. Recombinant Chlamydia trachomatis major outer membrane protein (Ct-MOMP), the most abundant constituent of the chlamydial outer membrane complex, is considered the most attractive candidate for subunit-based vaccine formulations. Unfortunately, Ct-MOMP is difficult to express in its native structure in the E. coli outer membrane (OM). Here, by co-expression of the Bam complex, we improved the expression and localization of recombinant Ct-MOMP in the E. coli OM. Under these conditions, recombinant Ct-MOMP appeared to assemble into a \u03b2-barrel conformation and express domains at the cell surface indicative of correct folding. The data indicate that limited availability of the Bam complex can be a bottleneck for the production of heterologous OM vaccine antigens, information that is also relevant for strategies aimed at producing recombinant OMV-based vaccines.<\/jats:p>","DOI":"10.3390\/ijms23137393","type":"journal-article","created":{"date-parts":[[2022,7,3]],"date-time":"2022-07-03T22:50:46Z","timestamp":1656888646000},"page":"7393","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Overexpression of the Bam Complex Improves the Production of Chlamydia trachomatis MOMP in the E. coli Outer Membrane"],"prefix":"10.3390","volume":"23","author":[{"given":"Dung T.","family":"Huynh","sequence":"first","affiliation":[{"name":"Abera Bioscience AB, 2141 Uppsala, Sweden"},{"name":"Department of Molecular Microbiology, Amsterdam Institute of Molecular and Life Sciences (AIMMS), Vrije Universiteit, 1081 HV Amsterdam, The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wouter S. P.","family":"Jong","sequence":"additional","affiliation":[{"name":"Abera Bioscience AB, 2141 Uppsala, Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gregory M.","family":"Koningstein","sequence":"additional","affiliation":[{"name":"Department of Molecular Microbiology, Amsterdam Institute of Molecular and Life Sciences (AIMMS), Vrije Universiteit, 1081 HV Amsterdam, The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2164-1611","authenticated-orcid":false,"given":"Peter","family":"van Ulsen","sequence":"additional","affiliation":[{"name":"Department of Molecular Microbiology, Amsterdam Institute of Molecular and Life Sciences (AIMMS), Vrije Universiteit, 1081 HV Amsterdam, The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joen","family":"Luirink","sequence":"additional","affiliation":[{"name":"Abera Bioscience AB, 2141 Uppsala, Sweden"},{"name":"Department of Molecular Microbiology, Amsterdam Institute of Molecular and Life Sciences (AIMMS), Vrije Universiteit, 1081 HV Amsterdam, The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,7,2]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"7289","DOI":"10.1016\/j.vaccine.2017.01.023","article-title":"Status of Vaccine Research and Development of Vaccines for Chlamydia Trachomatis Infection","volume":"37","author":"Poston","year":"2019","journal-title":"Vaccine"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"2965","DOI":"10.1016\/j.vaccine.2021.03.043","article-title":"Chlamydia Trachomatis: Cell Biology, Immunology and Vaccination","volume":"39","author":"Murray","year":"2021","journal-title":"Vaccine"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"385","DOI":"10.1038\/nrmicro.2016.30","article-title":"Chlamydia Cell Biology and Pathogenesis","volume":"14","author":"Elwell","year":"2016","journal-title":"Nat. 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