{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T18:50:28Z","timestamp":1791917428229,"version":"4.3.4"},"reference-count":44,"publisher":"MDPI AG","issue":"13","license":[{"start":{"date-parts":[[2022,7,4]],"date-time":"2022-07-04T00:00:00Z","timestamp":1656892800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"The \u201cJBGS\u201d Project of Seed Industry Revitalization in Jiangsu Province","award":["JBGS[2021]"],"award-info":[{"award-number":["JBGS[2021]"]}]},{"name":"The \u201cJBGS\u201d Project of Seed Industry Revitalization in Jiangsu Province","award":["CX(20)2011"],"award-info":[{"award-number":["CX(20)2011"]}]},{"name":"The \u201cJBGS\u201d Project of Seed Industry Revitalization in Jiangsu Province","award":["31972571"],"award-info":[{"award-number":["31972571"]}]},{"name":"The \u201cJBGS\u201d Project of Seed Industry Revitalization in Jiangsu Province","award":["T0201900482"],"award-info":[{"award-number":["T0201900482"]}]},{"name":"The Jiangsu Agriculture Science and Technology Innovation Fund","award":["JBGS[2021]"],"award-info":[{"award-number":["JBGS[2021]"]}]},{"name":"The Jiangsu Agriculture Science and Technology Innovation Fund","award":["CX(20)2011"],"award-info":[{"award-number":["CX(20)2011"]}]},{"name":"The Jiangsu Agriculture Science and Technology Innovation Fund","award":["31972571"],"award-info":[{"award-number":["31972571"]}]},{"name":"The Jiangsu Agriculture Science and Technology Innovation Fund","award":["T0201900482"],"award-info":[{"award-number":["T0201900482"]}]},{"name":"The National Natural Science Foundation of China","award":["JBGS[2021]"],"award-info":[{"award-number":["JBGS[2021]"]}]},{"name":"The National Natural Science Foundation of China","award":["CX(20)2011"],"award-info":[{"award-number":["CX(20)2011"]}]},{"name":"The National Natural Science Foundation of China","award":["31972571"],"award-info":[{"award-number":["31972571"]}]},{"name":"The National Natural Science Foundation of China","award":["T0201900482"],"award-info":[{"award-number":["T0201900482"]}]},{"name":"The modern molecular breeding technique for breeding high fertility and high quality lean porcine matching lines","award":["JBGS[2021]"],"award-info":[{"award-number":["JBGS[2021]"]}]},{"name":"The modern molecular breeding technique for breeding high fertility and high quality lean porcine matching lines","award":["CX(20)2011"],"award-info":[{"award-number":["CX(20)2011"]}]},{"name":"The modern molecular breeding technique for breeding high fertility and high quality lean porcine matching lines","award":["31972571"],"award-info":[{"award-number":["31972571"]}]},{"name":"The modern molecular breeding technique for breeding high fertility and high quality lean porcine matching lines","award":["T0201900482"],"award-info":[{"award-number":["T0201900482"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Glucose 6-P dehydrogenase (G6PD) is the first rate-limiting enzyme in pentose phosphate pathway (PPP), and it is proverbial that G6PD is absent in skeletal muscle. However, how and why G6PD is down-regulated during skeletal muscle development is unclear. In this study, we confirmed the expression of G6PD was down-regulated during myogenesis in vitro and in vivo. G6PD was absolutely silent in adult skeletal muscle. Histone H3 acetylation and DNA methylation act together on the expression of G6PD. Neither knock-down of G6PD nor over-expression of G6PD affects myogenic differentiation. Knock-down of G6PD significantly promotes the sensitivity and response of skeletal muscle cells to insulin; over-expression of G6PD significantly injures the sensitivity and response of skeletal muscle cells to insulin. High-fat diet treatment impairs insulin signaling by up-regulating G6PD, and knock-down of G6PD rescues the impaired insulin signaling and glucose uptake caused by high-fat diet treatment. Taken together, this study explored the importance of G6PD deficiency during myogenic differentiation, which provides new sight to treat insulin resistance and type-2 diabetes.<\/jats:p>","DOI":"10.3390\/ijms23137425","type":"journal-article","created":{"date-parts":[[2022,7,4]],"date-time":"2022-07-04T11:15:05Z","timestamp":1656933305000},"page":"7425","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["G6PD Deficiency Is Crucial for Insulin Signaling Activation in Skeletal Muscle"],"prefix":"10.3390","volume":"23","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8332-1824","authenticated-orcid":false,"given":"Aiwen","family":"Jiang","sequence":"first","affiliation":[{"name":"Department of Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongyun","family":"Guo","sequence":"additional","affiliation":[{"name":"Department of Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoyu","family":"Jiang","sequence":"additional","affiliation":[{"name":"Department of Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5761-6963","authenticated-orcid":false,"given":"Jingli","family":"Tao","sequence":"additional","affiliation":[{"name":"Department of Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wangjun","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Honglin","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,7,4]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1152\/jappl.2000.89.1.81","article-title":"Skeletal muscle mass and distribution in 468 men and women aged 18\u201388 yr","volume":"23","author":"Janssen","year":"2000","journal-title":"J. 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