{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T21:38:36Z","timestamp":1792013916312,"version":"4.4.0"},"reference-count":96,"publisher":"MDPI AG","issue":"14","license":[{"start":{"date-parts":[[2022,7,11]],"date-time":"2022-07-11T00:00:00Z","timestamp":1657497600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Fisheries Department of the Israel Ministry of Agriculture and Rural Development"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Oreochromis niloticus has been used as a reference genome for studies of tilapia sex determination (SD) revealing segregating genetic loci on linkage groups (LGs) 1, 3, and 23. The master key regulator genes (MKR) underlying the SD regions on LGs 3 and 23 have been already found. To identify the MKR in fish that segregate for the LG1 XX\/XY SD-system, we applied short variant discovery within the sequence reads of the genomic libraries of the Amherst hybrid stock, Coptodon zillii and Sarotherodon galilaeus, which were aligned to a 3-Mbp-region of the O. aureus genome. We obtained 66,372 variants of which six were concordant with the XX\/XY model of SD and were conserved across these species, disclosing the male specific figla-like gene. We further validated this observation in O. mossambicus and in the Chitralada hybrid stock. Genome alignment of the 1252-bp transcript showed that the figla-like gene\u2019s size was 2664 bp, and that its three exons were capable of encoding 99 amino acids including a 45-amino-acid basic helix\u2013loop\u2013helix domain that is typical of the ovary development regulator\u2014factor-in-the-germline-alpha (FIGLA). In Amherst gonads, the figla-like gene was exclusively expressed in testes. Thus, the figla-like genomic presence determines male fate by interrupting the female developmental program. This indicates that the figla-like gene is the long-sought SD MKR on LG1.<\/jats:p>","DOI":"10.3390\/ijms23147636","type":"journal-article","created":{"date-parts":[[2022,7,11]],"date-time":"2022-07-11T21:57:53Z","timestamp":1657576673000},"page":"7636","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Absence of Figla-like Gene Is Concordant with Femaleness in Cichlids Harboring the LG1 Sex-Determination System"],"prefix":"10.3390","volume":"23","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8038-8708","authenticated-orcid":false,"given":"Arie Yehuda","family":"Curzon","sequence":"first","affiliation":[{"name":"Institute of Animal Science, Agricultural Research Organization, Rishon LeTsiyon 75288, Israel"},{"name":"Robert H. Smith Faculty of Agriculture, Food and Environment, Hebrew University of Jerusalem, Rehovot 76100, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7772-8161","authenticated-orcid":false,"given":"Andrey","family":"Shirak","sequence":"additional","affiliation":[{"name":"Institute of Animal Science, Agricultural Research Organization, Rishon LeTsiyon 75288, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ayana","family":"Benet-Perlberg","sequence":"additional","affiliation":[{"name":"Dor Research Station, Division of Fishery and Aquaculture, Hof HaCarmel 30820, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alon","family":"Naor","sequence":"additional","affiliation":[{"name":"Dor Research Station, Division of Fishery and Aquaculture, Hof HaCarmel 30820, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shay Israel","family":"Low-Tanne","sequence":"additional","affiliation":[{"name":"Dor Research Station, Division of Fishery and Aquaculture, Hof HaCarmel 30820, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haled","family":"Sharkawi","sequence":"additional","affiliation":[{"name":"Dor Research Station, Division of Fishery and Aquaculture, Hof HaCarmel 30820, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Micha","family":"Ron","sequence":"additional","affiliation":[{"name":"Institute of Animal Science, Agricultural Research Organization, Rishon LeTsiyon 75288, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6191-8948","authenticated-orcid":false,"given":"Eyal","family":"Seroussi","sequence":"additional","affiliation":[{"name":"Institute of Animal Science, Agricultural Research Organization, Rishon LeTsiyon 75288, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,7,11]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1001","DOI":"10.1111\/j.1742-4658.2011.08028.x","article-title":"Vertebrate sex determination: Questioning the hierarchy","volume":"278","author":"Herpin","year":"2011","journal-title":"FEBS J."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"675","DOI":"10.1038\/nrg.2017.60","article-title":"Vertebrate sex determination: Evolutionary plasticity of a fundamental switch","volume":"18","author":"Capel","year":"2017","journal-title":"Nat. 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