{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,23]],"date-time":"2026-08-23T16:16:24Z","timestamp":1787501784285,"version":"build-2736575974"},"reference-count":32,"publisher":"MDPI AG","issue":"14","license":[{"start":{"date-parts":[[2022,7,11]],"date-time":"2022-07-11T00:00:00Z","timestamp":1657497600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Research Foundation of Korea (NRF)","award":["2021R1A2C101417611"],"award-info":[{"award-number":["2021R1A2C101417611"]}]},{"name":"National Research Foundation of Korea (NRF)","award":["BCRI 20-032"],"award-info":[{"award-number":["BCRI 20-032"]}]},{"name":"Chonnam National University Hospital Biomedical Research Institute","award":["2021R1A2C101417611"],"award-info":[{"award-number":["2021R1A2C101417611"]}]},{"name":"Chonnam National University Hospital Biomedical Research Institute","award":["BCRI 20-032"],"award-info":[{"award-number":["BCRI 20-032"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>(1) Objective: In order to evaluate the effect of a pre-induced mesenchymal stem cell (MSC)-coated cellulose\/collagen nanofibrous nerve conduit on facial nerve regeneration in a rat model both in vitro and in vivo. (2) Methods: After fabrication of the cellulose\/collagen nanofibrous conduit, its lumen was coated with either MSCs or pre-induced MSCs. The nerve conduit was then applied to the defective main trunk of the facial nerve. Rats were randomly divided into three treatment groups (n = 10 in each): cellulose\/collagen nanofiber (control group), cellulose\/collagen nanofiber\/MSCs (group I), and cellulose\/collagen nanofiber\/pre-induced MSCs (group II). (3) Results Fibrillation of the vibrissae of each group was observed, and action potential threshold was compared 8 weeks post-surgery. Histopathological changes were also observed. Groups I and II showed better recovery of vibrissa fibrillation than the control group. (4) Conclusions: Group II, treated with the pre-induced MSC-coated cellulose\/collagen nanofibrous nerve conduit, showed the highest degree of recovery based on functional and histological evaluations.<\/jats:p>","DOI":"10.3390\/ijms23147638","type":"journal-article","created":{"date-parts":[[2022,7,11]],"date-time":"2022-07-11T21:57:53Z","timestamp":1657576673000},"page":"7638","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Effect of Pre-Induced Mesenchymal Stem Cell-Coated Cellulose\/Collagen Nanofibrous Nerve Conduit on Regeneration of Transected Facial Nerve"],"prefix":"10.3390","volume":"23","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2149-5095","authenticated-orcid":false,"given":"GwangWon","family":"Cho","sequence":"first","affiliation":[{"name":"Department of Biology, College of Natural Science, Chosun University, Gwangju 61452, Korea"},{"name":"Department of Life Science, BK21-Plus Research Team for Bioactive Control Technology, Chosun University, Gwangju 61452, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2451-0374","authenticated-orcid":false,"given":"Changjong","family":"Moon","sequence":"additional","affiliation":[{"name":"Department of Veterinary Anatomy, College of Veterinary Medicine and BK21 FOUR Program, Chonnam National University, Gwangju 61186, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7043-8425","authenticated-orcid":false,"given":"Nagarajan","family":"Maharajan","sequence":"additional","affiliation":[{"name":"Department of Biology, College of Natural Science, Chosun University, Gwangju 61452, Korea"},{"name":"Department of Life Science, BK21-Plus Research Team for Bioactive Control Technology, Chosun University, Gwangju 61452, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mary Jasmin","family":"Ang","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, University of the Philippines Los Ba\u00f1os, Los Ba\u00f1os 4031, Philippines"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Minseong","family":"Kim","sequence":"additional","affiliation":[{"name":"Advanced Biomaterial Team, Medical Device Development Center, Daegu-Gyeongbuk Medical Innovation Foundation, Dong-gu 41061, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4084-2301","authenticated-orcid":false,"given":"Chul Ho","family":"Jang","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology, Chonnam National University Medical School, Gwangju 61469, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,7,11]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1007\/s00590-018-2344-2","article-title":"Current concepts in peripheral nerve surgery","volume":"29","author":"Beris","year":"2019","journal-title":"Eur. J. Orthop. Surg. Traumatol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1229","DOI":"10.1007\/s10570-017-1632-z","article-title":"Neural tissue regeneration by a gabapentin-loaded cellulose acetate\/gelatin wet-electrospun scaffold","volume":"25","author":"Farzamfar","year":"2018","journal-title":"Cellulose"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"16716","DOI":"10.1038\/s41598-018-34699-8","article-title":"Electrospun nerve guide conduits have the potential to bridge peripheral nerve injuries in vivo","volume":"8","author":"Frost","year":"2018","journal-title":"Sci. Rep."},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Urrutia, D.N., Caviedes, P., Mardones, R., Minguell, J.J., Vega-Letter, A.M., and Jofre, C.M. (2019). Comparative study of the neural differentiation capacity of mesenchymal stromal cells from different tissue sources: An approach for their use in neural regeneration therapies. PLoS ONE, 14.","DOI":"10.1371\/journal.pone.0213032"},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Li, C., Zhang, M., Liu, S.Y., Zhang, F.S., Wan, T., Ding, Z.T., and Zhang, P.X. (2021). Chitin Nerve Conduits with Three-Dimensional Spheroids of Mesenchymal Stem Cells from SD Rats Promote Peripheral Nerve Regeneration. Polymers, 13.","DOI":"10.3390\/polym13223957"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1470","DOI":"10.1007\/s12640-021-00391-5","article-title":"Bone Marrow Mesenchymal Stem Cell Condition Medium Loaded on PCL Nanofibrous Scaffold Promoted Nerve Regeneration After Sciatic Nerve Transection in Male Rats","volume":"39","author":"Raoofi","year":"2021","journal-title":"Neurotox. Res."},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Tanaka, H., Kakinoki, R., Kaizawa, Y., Yurie, H., Ikeguchi, R., and Akagi, M. (2021). Bone marrow-derived mesenchymal stem cells transplanted into a vascularized biodegradable tube containing decellularized allogenic nerve basal laminae promoted peripheral nerve regeneration; can it be an alternative of autologous nerve graft?. PLoS ONE, 16.","DOI":"10.1371\/journal.pone.0254968"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"2170","DOI":"10.4103\/1673-5374.310941","article-title":"Mesenchymal stem cell treatment for peripheral nerve injury: A narrative review","volume":"16","author":"Zhang","year":"2021","journal-title":"Neural. Regen. Res."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"34","DOI":"10.4062\/biomolther.2019.065","article-title":"Differentiation of human mesenchymal stem cells towards neuronal lineage: Clinical trials in nervous system disorders","volume":"28","author":"Perazzoli","year":"2020","journal-title":"Biomol. Ther."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"82","DOI":"10.2174\/157488811795495486","article-title":"Mesenchymal stem cells neuronal differentiation ability: A real perspective for nervous system repair?","volume":"6","author":"Scuteri","year":"2011","journal-title":"Curr. Stem Cell Res. Ther."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"440","DOI":"10.3858\/emm.2009.41.6.049","article-title":"Induction of unfolded protein response during neuronal induction of rat bone marrow stromal cells and mouse embryonic stem cells","volume":"41","author":"Cho","year":"2009","journal-title":"Exp. Mol. Med."},{"key":"ref_12","first-page":"848","article-title":"The Role of Stress Granules in the Neuronal Differentiation of Stem Cells","volume":"43","author":"Jeong","year":"2020","journal-title":"Mol. Cells"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1016\/j.jchemneu.2016.07.003","article-title":"Induction of mice adult bone marrow mesenchymal stem cells into functional motor neuron-like cells","volume":"77","author":"Abdullah","year":"2016","journal-title":"J. Chem. Neuroanat."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"625","DOI":"10.1080\/00207454.2020.1744592","article-title":"Basic fibroblast growth factor promotes human dental pulp stem cells cultured in 3D porous chitosan scaffolds to neural differentiation","volume":"131","author":"Zheng","year":"2021","journal-title":"Int. J. Neurosci."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"57","DOI":"10.4103\/1735-3327.321868","article-title":"Neurogenic induction of human dental pulp derived stem cells by hanging drop technique, basic fibroblast growth factor, and SHH factors","volume":"18","author":"Farhang","year":"2021","journal-title":"Dent. Res. J."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"224","DOI":"10.1089\/cell.2012.0077","article-title":"Effects of epidermal growth factor and basic fibroblast growth factor on the proliferation and osteogenic and neural differentiation of adipose-derived stem cells","volume":"15","author":"Hu","year":"2013","journal-title":"Cell. Reprogram."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"37381","DOI":"10.1021\/acsami.9b07053","article-title":"Triple PLGA\/PCL scaffold modification including silver impregnation, collagen coating, and electrospinning significantly improve biocompatibility, antimicrobial, and osteogenic properties for orofacial tissue regeneration","volume":"11","author":"Qian","year":"2019","journal-title":"ACS Appl. Mater. Interfaces"},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Han, Y., Li, X., Zhang, Y., Han, Y., Chang, F., and Ding, J. (2019). Mesenchymal Stem Cells for Regenerative Medicine. Cells, 8.","DOI":"10.3390\/cells8080886"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"193","DOI":"10.15283\/ijsc17036","article-title":"Proteomic Analysis of Human Adipose Derived Stem Cells during Small Molecule Chemical Stimulated Pre-neuronal Differentiation","volume":"10","author":"Santos","year":"2017","journal-title":"Int. J. Stem Cells"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1016\/j.neulet.2014.10.024","article-title":"Resveratrol-induced SIRT1 activation promotes neuronal differentiation of human bone marrow mesenchymal stem cells","volume":"584","author":"Joe","year":"2015","journal-title":"Neurosci. Lett."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1007\/s13770-019-00235-6","article-title":"Centella asiatica (L.)-Neurodifferentiated Mesenchymal Stem Cells Promote the Regeneration of Peripheral Nerve","volume":"17","author":"Hussin","year":"2020","journal-title":"Tissue Eng. Regen. Med."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"547","DOI":"10.1007\/s10517-021-05267-4","article-title":"Changes in the Thickness of Rat Nerve Sheaths after Single Subperineural Administration of Rat Bone Marrow Mesenchymal Stem Cells","volume":"171","author":"Petrova","year":"2021","journal-title":"Bull. Exp. Biol. Med."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"3521","DOI":"10.1113\/JP270874","article-title":"The repair Schwann cell and its function in regenerating nerves","volume":"594","author":"Jessen","year":"2016","journal-title":"J. Physiol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"a020487","DOI":"10.1101\/cshperspect.a020487","article-title":"Schwann Cells: Development and Role in Nerve Repair","volume":"7","author":"Jessen","year":"2015","journal-title":"Cold Spring Harb. Perspect. Biol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"608442","DOI":"10.3389\/fnmol.2020.608442","article-title":"Insights Into the Role and Potential of Schwann Cells for Peripheral Nerve Repair From Studies of Development and Injury","volume":"13","author":"Balakrishnan","year":"2020","journal-title":"Front. Mol. Neurosci."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"973","DOI":"10.1097\/NEN.0b013e3181eff6dc","article-title":"Human umbilical cord-derived mesenchymal stromal cells differentiate into functional Schwann cells that sustain peripheral nerve regeneration","volume":"69","author":"Matsuse","year":"2010","journal-title":"J. Neuropathol. Exp. Neurol."},{"key":"ref_27","first-page":"113","article-title":"Mesenchymal stem cells as an alternative for Schwann cells in rat spinal cord injury","volume":"17","author":"Zaminy","year":"2013","journal-title":"Iran. Biomed. J."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"28","DOI":"10.29252\/beat-070104","article-title":"Effect of Local Administration of Verapamil Combined with Chitosan Based Hybrid Nanofiber Conduit on Transected Sciatic Nerve in Rat","volume":"7","author":"Raisi","year":"2019","journal-title":"Bull. Emerg. Trauma"},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"D\u0119bski, T., Kije\u0144ska-Gawro\u0144ska, E., Zo\u0142oci\u0144ska, A., Siennicka, K., S\u0142ysz, A., Paskal, W., W\u0142odarski, P.K., \u015awi\u0119szkowski, W., and Pojda, Z. (2021). Bioactive Nanofiber-Based Conduits in a Peripheral Nerve Gap Management-An Animal Model Study. Int. J. Mol. Sci., 22.","DOI":"10.3390\/ijms22115588"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"746","DOI":"10.1007\/s12031-020-01694-6","article-title":"Regeneration of Rat Sciatic Nerve Using PLGA Conduit Containing Rat ADSCs with Controlled Release of BDNF and Gold Nanoparticles","volume":"71","author":"Jahromi","year":"2021","journal-title":"J. Mol. Neurosci."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1590","DOI":"10.7150\/thno.36272","article-title":"Aligned chitosan nanofiber hydrogel grafted with peptides mimicking bioactive brain-derived neurotrophic factor and vascular endothelial growth factor repair long-distance sciatic nerve defects in rats","volume":"10","author":"Rao","year":"2020","journal-title":"Theranostics"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"495","DOI":"10.5607\/en.2019.28.4.495","article-title":"Memantine Attenuates Salicylate-induced Tinnitus Possibly by Reducing NR2B Expression in Auditory Cortex of Rat","volume":"28","author":"Jang","year":"2019","journal-title":"Exp. Neurobiol."}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/23\/14\/7638\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T23:47:58Z","timestamp":1760140078000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/23\/14\/7638"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,7,11]]},"references-count":32,"journal-issue":{"issue":"14","published-online":{"date-parts":[[2022,7]]}},"alternative-id":["ijms23147638"],"URL":"https:\/\/doi.org\/10.3390\/ijms23147638","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,7,11]]}}}