{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,23]],"date-time":"2026-04-23T20:57:41Z","timestamp":1776977861542,"version":"3.51.4"},"reference-count":46,"publisher":"MDPI AG","issue":"14","license":[{"start":{"date-parts":[[2022,7,12]],"date-time":"2022-07-12T00:00:00Z","timestamp":1657584000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Science Center in Poland","award":["2016\/21\/B\/NZ7\/01081"],"award-info":[{"award-number":["2016\/21\/B\/NZ7\/01081"]}]},{"name":"National Science Center in Poland","award":["POWR.03.02.00-00-013\/16"],"award-info":[{"award-number":["POWR.03.02.00-00-013\/16"]}]},{"name":"National Science Center in Poland","award":["ANR-11-LABX-0029"],"award-info":[{"award-number":["ANR-11-LABX-0029"]}]},{"name":"National Science Center in Poland","award":["ANR-11-LABX-0018-01"],"award-info":[{"award-number":["ANR-11-LABX-0018-01"]}]},{"name":"InterDokMed","award":["2016\/21\/B\/NZ7\/01081"],"award-info":[{"award-number":["2016\/21\/B\/NZ7\/01081"]}]},{"name":"InterDokMed","award":["POWR.03.02.00-00-013\/16"],"award-info":[{"award-number":["POWR.03.02.00-00-013\/16"]}]},{"name":"InterDokMed","award":["ANR-11-LABX-0029"],"award-info":[{"award-number":["ANR-11-LABX-0029"]}]},{"name":"InterDokMed","award":["ANR-11-LABX-0018-01"],"award-info":[{"award-number":["ANR-11-LABX-0018-01"]}]},{"name":"Labex SynOrg","award":["2016\/21\/B\/NZ7\/01081"],"award-info":[{"award-number":["2016\/21\/B\/NZ7\/01081"]}]},{"name":"Labex SynOrg","award":["POWR.03.02.00-00-013\/16"],"award-info":[{"award-number":["POWR.03.02.00-00-013\/16"]}]},{"name":"Labex SynOrg","award":["ANR-11-LABX-0029"],"award-info":[{"award-number":["ANR-11-LABX-0029"]}]},{"name":"Labex SynOrg","award":["ANR-11-LABX-0018-01"],"award-info":[{"award-number":["ANR-11-LABX-0018-01"]}]},{"name":"Labex IRON","award":["2016\/21\/B\/NZ7\/01081"],"award-info":[{"award-number":["2016\/21\/B\/NZ7\/01081"]}]},{"name":"Labex IRON","award":["POWR.03.02.00-00-013\/16"],"award-info":[{"award-number":["POWR.03.02.00-00-013\/16"]}]},{"name":"Labex IRON","award":["ANR-11-LABX-0029"],"award-info":[{"award-number":["ANR-11-LABX-0029"]}]},{"name":"Labex IRON","award":["ANR-11-LABX-0018-01"],"award-info":[{"award-number":["ANR-11-LABX-0018-01"]}]},{"name":"FEDER TECHSAB","award":["2016\/21\/B\/NZ7\/01081"],"award-info":[{"award-number":["2016\/21\/B\/NZ7\/01081"]}]},{"name":"FEDER TECHSAB","award":["POWR.03.02.00-00-013\/16"],"award-info":[{"award-number":["POWR.03.02.00-00-013\/16"]}]},{"name":"FEDER TECHSAB","award":["ANR-11-LABX-0029"],"award-info":[{"award-number":["ANR-11-LABX-0029"]}]},{"name":"FEDER TECHSAB","award":["ANR-11-LABX-0018-01"],"award-info":[{"award-number":["ANR-11-LABX-0018-01"]}]},{"name":"University of Orleans","award":["2016\/21\/B\/NZ7\/01081"],"award-info":[{"award-number":["2016\/21\/B\/NZ7\/01081"]}]},{"name":"University of Orleans","award":["POWR.03.02.00-00-013\/16"],"award-info":[{"award-number":["POWR.03.02.00-00-013\/16"]}]},{"name":"University of Orleans","award":["ANR-11-LABX-0029"],"award-info":[{"award-number":["ANR-11-LABX-0029"]}]},{"name":"University of Orleans","award":["ANR-11-LABX-0018-01"],"award-info":[{"award-number":["ANR-11-LABX-0018-01"]}]},{"name":"Centre-Val de Loire Region","award":["2016\/21\/B\/NZ7\/01081"],"award-info":[{"award-number":["2016\/21\/B\/NZ7\/01081"]}]},{"name":"Centre-Val de Loire Region","award":["POWR.03.02.00-00-013\/16"],"award-info":[{"award-number":["POWR.03.02.00-00-013\/16"]}]},{"name":"Centre-Val de Loire Region","award":["ANR-11-LABX-0029"],"award-info":[{"award-number":["ANR-11-LABX-0029"]}]},{"name":"Centre-Val de Loire Region","award":["ANR-11-LABX-0018-01"],"award-info":[{"award-number":["ANR-11-LABX-0018-01"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>The use of polypyridyl Ru complexes to inhibit metastasis is a novel approach, and recent studies have shown promising results. We have reported recently that Ru (II) complexes gathering two 4,7-diphenyl-1,10-phenanthroline (dip) ligands and the one being 2,2\u2032-bipyridine (bpy) or its derivative with a 4-[3-(2-nitro-1H-imidazol-1-yl)propyl (bpy-NitroIm) or 5-(4-{4\u2032-methyl-[2,2\u2032-bipyridine]-4-yl}but-1-yn-1-yl)pyridine-2-carbaldehyde semicarbazone (bpy-SC) moieties can alter the metastatic cascade, among others, by modulating cell adhesion properties. In this work, we show further studies of this group of complexes by evaluating their effect on HMEC-1 endothelial cells. While all the tested complexes significantly inhibited the endothelial cell migration, Ru-bpy additionally interrupted the pseudovessels formation. Functional changes in endothelial cells might arise from the impact of the studied compounds on cell elasticity and expression of proteins (vinculin and paxillin) involved in focal adhesions. Furthermore, molecular studies showed that complexes modulate the expression of cell adhesion molecules, which has been suggested to be one of the factors that mediate the activation of angiogenesis. Based on the performed studies, we can conclude that the investigated polypyridyl Ru (II) complexes can deregulate the functionality of endothelial cells which may lead to the inhibition of angiogenesis.<\/jats:p>","DOI":"10.3390\/ijms23147708","type":"journal-article","created":{"date-parts":[[2022,7,12]],"date-time":"2022-07-12T20:52:41Z","timestamp":1657659161000},"page":"7708","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Impact of Polypyridyl Ru Complexes on Angiogenesis\u2014Contribution to Their Antimetastatic Activity"],"prefix":"10.3390","volume":"23","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0462-6492","authenticated-orcid":false,"given":"Ilona","family":"Gurgul","sequence":"first","affiliation":[{"name":"Faculty of Chemistry, Jagiellonian University in Krakow, Gronostajowa 2, 30-387 Krakow, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6973-3614","authenticated-orcid":false,"given":"Olga","family":"Mazuryk","sequence":"additional","affiliation":[{"name":"Faculty of Chemistry, Jagiellonian University in Krakow, Gronostajowa 2, 30-387 Krakow, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kamila","family":"Stachyra","sequence":"additional","affiliation":[{"name":"Chair of Pharmacology, Faculty of Medicine, Jagiellonian University Medical College, Grzegorzecka 16, 31-531 Krakow, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rafa\u0142","family":"Olszanecki","sequence":"additional","affiliation":[{"name":"Chair of Pharmacology, Faculty of Medicine, Jagiellonian University Medical College, Grzegorzecka 16, 31-531 Krakow, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0844-8662","authenticated-orcid":false,"given":"Ma\u0142gorzata","family":"Lekka","sequence":"additional","affiliation":[{"name":"Department of Biophysical Microstructures, Institute of Nuclear Physics, Polish Academy of Sciences, PL-31342 Krakow, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4847-3520","authenticated-orcid":false,"given":"Micha\u0142","family":"\u0141omzik","sequence":"additional","affiliation":[{"name":"Faculty of Chemistry, Jagiellonian University in Krakow, Gronostajowa 2, 30-387 Krakow, Poland"},{"name":"Department of Organic Chemistry, Faculty of Chemistry, University of \u0141\u00f3d\u017a, ul. Tamka 12, 91-403 \u0141\u00f3d\u017a, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1394-1603","authenticated-orcid":false,"given":"Franck","family":"Suzenet","sequence":"additional","affiliation":[{"name":"Institute of Organic and Analytical Chemistry, University of Orl\u00e9ans, UMR-CNRS 7311, rue de Chartres, BP 6759, CEDEX 2, 45067 Orl\u00e9ans, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4905-1581","authenticated-orcid":false,"given":"Philippe C.","family":"Gros","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Lorraine, CNRS, L2CM, F-54000 Nancy, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9827-4661","authenticated-orcid":false,"given":"Ma\u0142gorzata","family":"Brindell","sequence":"additional","affiliation":[{"name":"Faculty of Chemistry, Jagiellonian University in Krakow, Gronostajowa 2, 30-387 Krakow, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,7,12]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"7706","DOI":"10.1039\/C7CS00680B","article-title":"The Development of Ruthenium(Ii) Polypyridyl Complexes and Conjugates for in vitro Cellular and in vivo Applications","volume":"46","author":"Poynton","year":"2017","journal-title":"Chem. Soc. Rev."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"9904","DOI":"10.1039\/C9CC04098F","article-title":"Functionalization and Cancer-Targeting Design of Ruthenium Complexes for Precise Cancer Therapy","volume":"55","author":"Liu","year":"2019","journal-title":"Chem. Commun."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"213736","DOI":"10.1016\/j.ccr.2020.213736","article-title":"Ruthenium Polypyridyl Complex-Containing Bioconjugates","volume":"434","author":"Gasser","year":"2021","journal-title":"Coord. Chem. Rev."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1138","DOI":"10.1021\/acs.chemrev.8b00209","article-title":"Stimuli-Responsive Therapeutic Metallodrugs","volume":"119","author":"Wang","year":"2019","journal-title":"Chem. Rev."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"8818","DOI":"10.1039\/C5CS00134J","article-title":"Linking the Future of Anticancer Metal-Complexes to the Therapy of Tumour Metastases","volume":"44","author":"Bergamo","year":"2015","journal-title":"Chem. Soc. Rev."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"111652","DOI":"10.1016\/j.jinorgbio.2021.111652","article-title":"Moving Ru Polypyridyl Complexes beyond Cytotoxic Activity towards Metastasis Inhibition","volume":"226","author":"Brindell","year":"2022","journal-title":"J. Inorg. Biochem."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1549","DOI":"10.1002\/ejic.201600986","article-title":"Thirty Years of the Drug Candidate NAMI-A and the Myths in the Field of Ruthenium Anticancer Compounds: A Personal Perspective","volume":"2017","author":"Alessio","year":"2017","journal-title":"Eur. J. Inorg. Chem."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"5861","DOI":"10.1046\/j.1432-1033.2002.03307.x","article-title":"Inhibition of the MEK\/ERK Signaling Pathway by the Novel Antimetastatic Agent NAMI-A down Regulates c-Myc Gene Expression and Endothelial Cell Proliferation","volume":"269","author":"Pintus","year":"2002","journal-title":"Eur. J. Biochem."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1035","DOI":"10.1007\/s10534-016-9974-9","article-title":"Hypoxia-Selective Inhibition of Angiogenesis Development by NAMI-A Analogues","volume":"29","author":"Oszajca","year":"2016","journal-title":"BioMetals"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"553","DOI":"10.1039\/C5MT00037H","article-title":"The Biological Effect of the Nitroimidazole Derivative of a Polypyridyl Ruthenium Complex on Cancer and Endothelial Cells","volume":"7","author":"Mazuryk","year":"2015","journal-title":"Metallomics"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"9157","DOI":"10.1038\/srep09157","article-title":"Ruthenium Polypyridyl Complex Inhibits Growth and Metastasis of Breast Cancer Cells by Suppressing FAK Signaling with Enhancement of TRAIL-Induced Apoptosis","volume":"5","author":"Cao","year":"2015","journal-title":"Sci. Rep."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1016\/j.csbj.2018.11.010","article-title":"Nucleus-Enriched Ruthenium Polypyridine Complex Acts as a Potent Inhibitor to Suppress Triple-Negative Breast Cancer Metastasis In Vivo","volume":"17","author":"Zhao","year":"2019","journal-title":"Comput. Struct. Biotechnol. J."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"784","DOI":"10.1039\/d0mt00019a","article-title":"Unexplored Features of Ru(Ii) Polypyridyl Complexes\u2014Towards Combined Cytotoxic and Antimetastatic Activity","volume":"12","author":"Gurgul","year":"2020","journal-title":"Metallomics"},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Gurgul, I., Janczy-Cempa, E., Mazuryk, O., Lekka, M., \u0141omzik, M., Suzenet, F., and Gros, P.C. (2022). Ma\u0142gorzata Brindell Inhibition of Metastasis by Polypyridyl Ru(II) Complexes through Modification of Cancer Cell Adhesion\u2014In Vitro Functional and Molecular Studies. J. Med. Chem.","DOI":"10.1021\/acs.jmedchem.2c00580"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1007\/s10549-011-1499-x","article-title":"Signaling Mechanism of Cell Adhesion Molecules in Breast Cancer Metastasis: Potential Therapeutic Targets","volume":"128","author":"Li","year":"2011","journal-title":"Breast Cancer Res. Treat."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1104","DOI":"10.1161\/hq0701.093685","article-title":"Role of the Matrix Metalloproteinase and Plasminogen Activator\u2013Plasmin Systems in Angiogenesis","volume":"21","author":"Pepper","year":"2001","journal-title":"Arterioscler. Thromb. Vasc. Biol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"684","DOI":"10.1186\/s12935-021-02379-9","article-title":"Cervical Cancer-Derived Exosomal MiR-663b Promotes Angiogenesis by Inhibiting Vinculin Expression in Vascular Endothelial Cells","volume":"21","author":"You","year":"2021","journal-title":"Cancer Cell Int."},{"key":"ref_18","first-page":"1111","article-title":"Endothelial Intercellular Adhesion Molecule I Expression Is Suppressed in Human: The Role of Angiogenic Factors","volume":"56","author":"Griffioen","year":"1996","journal-title":"Cancer Res."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"712","DOI":"10.1111\/bph.12344","article-title":"Angiogenesis Inhibitors in Cancer Therapy: Mechanistic Perspective on Classification and Treatment Rationales","volume":"170","year":"2013","journal-title":"Br. J. Pharmacol."},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Lopes-Coelho, F., Martins, F., Pereira, S.A., and Serpa, J. (2021). Anti-Angiogenic Therapy: Current Challenges and Future Perspectives. Int. J. Mol. Sci., 22.","DOI":"10.3390\/ijms22073765"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"775","DOI":"10.1016\/j.biopha.2018.12.022","article-title":"Therapeutic Targeting of Angiogenesis Molecular Pathways in Angiogenesis-Dependent Diseases","volume":"110","author":"Fallah","year":"2019","journal-title":"Biomed. Pharmacother."},{"key":"ref_22","first-page":"9","article-title":"Angiogenesis Inhibitors in Cancer-Mechanisms of Action","volume":"29","author":"Clarke","year":"2006","journal-title":"Exp. Clin. Pharmacol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.bbrc.2007.01.123","article-title":"Molecular Pathways of Angiogenesis Inhibition","volume":"355","author":"Tabruyn","year":"2007","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"52","DOI":"10.1016\/j.cell.2010.03.015","article-title":"Matrix Metalloproteinases: Regulators of the Tumor Microenvironment","volume":"141","author":"Kessenbrock","year":"2010","journal-title":"Cell"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1370","DOI":"10.3389\/fonc.2019.01370","article-title":"Role of Matrix Metalloproteinases in Angiogenesis and Cancer","volume":"9","author":"Arreola","year":"2019","journal-title":"Front. Oncol."},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Gajda-Morszewski, P., Gurgul, I., Janczy-Cempa, E., Mazuryk, O., \u0141omzik, M., and Brindell, M. (2021). Inhibition of Matrix Metalloproteinases and Cancer Cell Detachment by Ru(II) Polypyridyl Complexes Containing 4,7-Diphenyl-1,10-Phenanthroline Ligands-New Candidates for Antimetastatic Agents. Pharmaceuticals, 14.","DOI":"10.3390\/ph14101014"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"851","DOI":"10.1007\/s10637-014-0129-y","article-title":"Effects of the Combination of TRC105 and Bevacizumab on Endothelial Cell Biology","volume":"32","author":"Liu","year":"2014","journal-title":"Investig. New Drugs"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"R66","DOI":"10.1186\/bcr2903","article-title":"Sunitinib Inhibits Lymphatic Endothelial Cell Functions and Lymph Node Metastasis in a Breast Cancer Model through Inhibition of Vascular Endothelial Growth Factor Receptor 3","volume":"13","author":"Kodera","year":"2011","journal-title":"Breast Cancer Res."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1085","DOI":"10.1177\/1947601911432334","article-title":"Molecular Mechanisms of Tumor Angiogenesis","volume":"2","author":"Ziyad","year":"2011","journal-title":"Genes Cancer"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1953","DOI":"10.1016\/j.bbagen.2016.06.010","article-title":"Cell Stiffness Determined by Atomic Force Microscopy and Its Correlation with Cell Motility","volume":"1860","author":"Luo","year":"2016","journal-title":"Biochim. Biophys. Acta Gen. Subj."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"45575","DOI":"10.1038\/srep45575","article-title":"On the Determination of Elastic Moduli of Cells by AFM Based Indentation","volume":"7","author":"Ding","year":"2017","journal-title":"Sci. Rep."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"312","DOI":"10.1007\/s002490050213","article-title":"Elasticity of Normal and Cancerous Human Bladder Cells Studied by Scanning Force Microscopy","volume":"28","author":"Lekka","year":"1999","journal-title":"Eur. Biophys. J."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"1672","DOI":"10.1242\/jcs.132316","article-title":"Paxillin Controls Endothelial Cell Migration and Tumor Angiogenesis by Altering Neuropilin 2 Expression","volume":"127","author":"German","year":"2014","journal-title":"J. Cell Sci."},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Kong, D.-H., Kim, Y., Kim, M., Jang, J., and Lee, S. (2018). Emerging Roles of Vascular Cell Adhesion Molecule-1 (VCAM-1) in Immunological Disorders and Cancer. Int. J. Mol. Sci., 19.","DOI":"10.3390\/ijms19041057"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1409","DOI":"10.3748\/wjg.v9.i7.1409","article-title":"Association of VCAM-1 Overexpression with Oncogenesis, Tumor Angiogenesis and Metastasis of Gastric Carcinoma","volume":"9","author":"Ding","year":"2003","journal-title":"World J. Gastroenterol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1007\/s13402-017-0324-x","article-title":"Breast Cancer Metastasis: Putative Therapeutic Role of Vascular Cell Adhesion Molecule-1","volume":"40","author":"Sharma","year":"2017","journal-title":"Cell. Oncol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"455","DOI":"10.1016\/j.ygyno.2013.12.031","article-title":"Inhibition of A4\u03b21 Integrin Increases Ovarian Cancer Response to Carboplatin","volume":"132","author":"Scalici","year":"2014","journal-title":"Gynecol. Oncol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"4099","DOI":"10.1021\/ic00296a006","article-title":"Contribution from the Dipartimento Di Cis-and Trans-Dihalotetrakis(Dimethyl Sulfoxide)Ruthenium(II) Complexes (RuX2(DMSO)4; X = Cl, Br): Synthesis, Structure, and Antitumor Activity","volume":"27","author":"Chimiche","year":"1988","journal-title":"Inorg. Chem."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"4867","DOI":"10.1038\/s41467-021-25189-z","article-title":"ICAM1 Initiates CTC Cluster Formation and Trans-Endothelial Migration in Lung Metastasis of Breast Cancer","volume":"12","author":"Taftaf","year":"2021","journal-title":"Nat. Commun."},{"key":"ref_40","doi-asserted-by":"crossref","unstructured":"Ghislin, S., Obino, D., Middendorp, S., Boggetto, N., Alcaide-Loridan, C., and Deshayes, F. (2012). LFA-1 and ICAM-1 Expression Induced during Melanoma-Endothelial Cell Co-Culture Favors the Transendothelial Migration of Melanoma Cell Lines in Vitro. BMC Cancer, 12.","DOI":"10.1186\/1471-2407-12-455"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"4591","DOI":"10.1096\/fj.11-202747","article-title":"Melanoma Upregulates ICAM-1 Expression on Endothelial Cells through Engagement of Tumor CD44 with Endothelial E-selectin and Activation of a PKC\u03b1\u2013P38-SP-1 Pathway","volume":"28","author":"Zhang","year":"2014","journal-title":"FASEB J."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"1305","DOI":"10.1007\/s00775-014-1187-5","article-title":"Multifaceted Interplay between Lipophilicity, Protein Interaction and Luminescence Parameters of Non-Intercalative Ruthenium(II) Polypyridyl Complexes Controlling Cellular Imaging and Cytotoxic Properties","volume":"19","author":"Mazuryk","year":"2014","journal-title":"J. Biol. Inorg. Chem."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"80","DOI":"10.1016\/j.jinorgbio.2017.07.006","article-title":"New Ruthenium Compounds Bearing Semicarbazone 2-Formylopyridine Moiety: Playing with Auxiliary Ligands for Tuning the Mechanism of Biological Activity","volume":"175","author":"Mazuryk","year":"2017","journal-title":"J. Inorg. Biochem."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1016\/j.jinorgbio.2014.02.001","article-title":"2-Nitroimidazole-Ruthenium Polypyridyl Complex as a New Conjugate for Cancer Treatment and Visualization","volume":"134","author":"Mazuryk","year":"2014","journal-title":"J. Inorg. Biochem."},{"key":"ref_45","unstructured":"Carpentier, G. (2012). Contribution: Angiogenesis Analyzer. ImageJ News, 5."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"11568","DOI":"10.1038\/s41598-020-67289-8","article-title":"Angiogenesis Analyzer for ImageJ\u2014A comparative morphometric analysis of \u201cEndothelial Tube Formation Assay\u201d and \u201cFibrin Bead Assay\u201d","volume":"10","author":"Carpentier","year":"2020","journal-title":"Sci. Rep."}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/23\/14\/7708\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T23:49:01Z","timestamp":1760140141000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/23\/14\/7708"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,7,12]]},"references-count":46,"journal-issue":{"issue":"14","published-online":{"date-parts":[[2022,7]]}},"alternative-id":["ijms23147708"],"URL":"https:\/\/doi.org\/10.3390\/ijms23147708","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,7,12]]}}}