{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,11]],"date-time":"2026-02-11T18:03:27Z","timestamp":1770833007974,"version":"3.50.1"},"reference-count":27,"publisher":"MDPI AG","issue":"14","license":[{"start":{"date-parts":[[2022,7,15]],"date-time":"2022-07-15T00:00:00Z","timestamp":1657843200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea (NRF)","doi-asserted-by":"publisher","award":["NRF-2019R1I1A1A01043181"],"award-info":[{"award-number":["NRF-2019R1I1A1A01043181"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea (NRF)","doi-asserted-by":"publisher","award":["22202MFDS178"],"award-info":[{"award-number":["22202MFDS178"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Korea government (MFDS)","award":["NRF-2019R1I1A1A01043181"],"award-info":[{"award-number":["NRF-2019R1I1A1A01043181"]}]},{"name":"Korea government (MFDS)","award":["22202MFDS178"],"award-info":[{"award-number":["22202MFDS178"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Iodotyrosine deiodinase (IYD) is a type of deiodinase enzyme that scavenges iodide from the thyroid gland. Previously, we showed that H3 Ab acts as an agonist on IYD to induce migration of cells to the heart and differentiate human stem cells into brown adipocyte-like cells. To continue this study, we investigated the dual function of IYD in hypothyroidism by blocking IYD and in thermogenesis by looking at the induction of brown adipocyte-like cells by treatment with H3 Ab in a mouse model. Surprisingly, our results suggest H3 Ab acts on IYD as both an antagonist and agonist to reduce T4 and increase core body temperature in the mouse model. Taken together, the data suggest IYD has a dual function that can regulate physiological metabolism and enhance thermogenesis.<\/jats:p>","DOI":"10.3390\/ijms23147834","type":"journal-article","created":{"date-parts":[[2022,7,17]],"date-time":"2022-07-17T22:57:06Z","timestamp":1658098626000},"page":"7834","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Antibodies Regulate Dual-Function Enzyme IYD to Induce Functional Synergy between Metabolism and Thermogenesis"],"prefix":"10.3390","volume":"23","author":[{"given":"Sunghyun","family":"Kang","sequence":"first","affiliation":[{"name":"Disease Target Structure Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 34141, Korea"},{"name":"T-mac Co., Ltd., Daejeon 34141, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8029-2004","authenticated-orcid":false,"given":"Hwan-Woo","family":"Park","sequence":"additional","affiliation":[{"name":"Department of Cell Biology, Konyang University College of Medicine, Daejeon 35365, Korea"},{"name":"Myunggok Medical Research Institute, Konyang University College of Medicine, Daejeon 35365, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kyung Ho","family":"Han","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences and Biotechnology, Hannam University, Daejeon 34054, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,7,15]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"967","DOI":"10.1016\/j.celrep.2017.12.090","article-title":"Ceramide Synthase Schlank Is a Transcriptional Regulator Adapting Gene Expression to Energy Requirements","volume":"22","author":"Sociale","year":"2018","journal-title":"Cell Rep."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1252","DOI":"10.1007\/s11064-015-1590-5","article-title":"Insights into Enzyme Catalysis and Thyroid Hormone Regulation of Cerebral Ketimine Reductase\/mu-Crystallin Under Physiological Conditions","volume":"40","author":"Hallen","year":"2015","journal-title":"Neurochem. Res."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"5533","DOI":"10.1073\/pnas.1818824116","article-title":"Spastin is a dual-function enzyme that severs microtubules and promotes their regrowth to increase the number and mass of microtubules","volume":"116","author":"Kuo","year":"2019","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"454","DOI":"10.1080\/19420862.2016.1143182","article-title":"Inhibition of CD73 AMP hydrolysis by a therapeutic antibody with a dual, non-competitive mechanism of action","volume":"8","author":"Geoghegan","year":"2016","journal-title":"MAbs"},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Han, K.H., Arlian, B.M., Lin, C.W., Jin, H.Y., Kang, G.H., Lee, S., Lee, P.C., and Lerner, R.A. (2020). Agonist Antibody Converts Stem Cells into Migrating Brown Adipocyte-Like Cells in Heart. Cells, 9.","DOI":"10.3390\/cells9010256"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"E372","DOI":"10.1073\/pnas.1719259115","article-title":"Migration-based selections of antibodies that convert bone marrow into trafficking microglia-like cells that reduce brain amyloid beta","volume":"115","author":"Han","year":"2018","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"738","DOI":"10.1096\/fj.15-281329","article-title":"An agonist antibody that blocks autoimmunity by inducing anti-inflammatory macrophages","volume":"30","author":"Han","year":"2016","journal-title":"FASEB J."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"6474","DOI":"10.1021\/acsbiomaterials.9b00994","article-title":"VEGFR2-Specific Ligands Based on Affibody Molecules Demonstrate Agonistic Effects when Tetrameric in the Soluble Form or Immobilized via Spider Silk","volume":"5","author":"Guler","year":"2019","journal-title":"ACS Biomater. Sci. Eng."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"86","DOI":"10.1007\/s12020-022-03054-3","article-title":"Mutational screening of the TPO and DUOX2 genes in Argentinian children with congenital hypothyroidism due to thyroid dyshormonogenesis","volume":"77","author":"Molina","year":"2022","journal-title":"Endocrine"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"106227","DOI":"10.1016\/j.aquatox.2022.106227","article-title":"Cross-species comparison of chemical inhibition of human and Xenopus iodotyrosine deiodinase","volume":"249","author":"Olker","year":"2022","journal-title":"Aquat. Toxicol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1550","DOI":"10.1016\/S0140-6736(17)30703-1","article-title":"Hypothyroidism","volume":"390","author":"Chaker","year":"2017","journal-title":"Lancet"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"118","DOI":"10.1210\/er.2018-00168","article-title":"Gastrointestinal Malabsorption of Thyroxine","volume":"40","author":"Virili","year":"2019","journal-title":"Endocr. Rev."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"E1276","DOI":"10.1210\/jc.2011-3314","article-title":"Iodotyrosine deiodinase defect identified via genome-wide approach","volume":"97","author":"Burniat","year":"2012","journal-title":"J. Clin. Endocrinol. Metab."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"272","DOI":"10.1007\/s12011-015-0328-1","article-title":"Expression of Iodotyrosine Deiodinase in Thyroid and Other Organs in Iodine-Deficient and Iodine-Excess Rats","volume":"167","author":"Sun","year":"2015","journal-title":"Biol. Trace Elem. Res."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1210\/er.2009-0007","article-title":"Molecular aspects of thyroid hormone actions","volume":"31","author":"Cheng","year":"2010","journal-title":"Endocr. Rev."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"355","DOI":"10.1152\/physrev.00030.2013","article-title":"Thyroid hormone regulation of metabolism","volume":"94","author":"Mullur","year":"2014","journal-title":"Physiol. Rev."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"742","DOI":"10.1002\/hep.31296","article-title":"Thyroid Hormone Signaling and the Liver","volume":"72","author":"Ritter","year":"2020","journal-title":"Hepatology"},{"key":"ref_18","unstructured":"Rousset, B., Dupuy, C., Miot, F., and Dumont, F. (2000). Chapter 2 Thyroid Hormone Synthesis And Secretion, MDText.com, Inc."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1038\/s41572-022-00357-7","article-title":"Hypothyroidism","volume":"8","author":"Chaker","year":"2022","journal-title":"Nat. Rev. Dis. Primers"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"230","DOI":"10.1038\/s41574-021-00625-8","article-title":"Primary hypothyroidism and quality of life","volume":"18","author":"Hegedus","year":"2022","journal-title":"Nat. Rev. Endocrinol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"787535","DOI":"10.3389\/fphys.2021.787535","article-title":"Lipid Transport in Brown Adipocyte Thermogenesis","volume":"12","author":"Wade","year":"2021","journal-title":"Front. Physiol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"393","DOI":"10.1038\/s41580-021-00350-0","article-title":"The cellular and functional complexity of thermogenic fat","volume":"22","author":"Cohen","year":"2021","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"784","DOI":"10.1016\/j.stem.2020.07.013","article-title":"Generation of Functional Brown Adipocytes from Human Pluripotent Stem Cells via Progression through a Paraxial Mesoderm State","volume":"27","author":"Zhang","year":"2020","journal-title":"Cell Stem Cell"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"1874","DOI":"10.2337\/db19-0308","article-title":"Cellular Origins of Beige Fat Cells Revisited","volume":"68","author":"Shao","year":"2019","journal-title":"Diabetes"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"17970","DOI":"10.1073\/pnas.1906512116","article-title":"Diverse repertoire of human adipocyte subtypes develops from transcriptionally distinct mesenchymal progenitor cells","volume":"116","author":"Min","year":"2019","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"840","DOI":"10.4093\/dmj.2020.0291","article-title":"Brown Fat as a Regulator of Systemic Metabolism beyond Thermogenesis","volume":"45","author":"Yuko","year":"2021","journal-title":"Diabetes Metab. J."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"4894","DOI":"10.1210\/jc.2008-0865","article-title":"Molecular characterization of iodotyrosine dehalogenase deficiency in patients with hypothyroidism","volume":"93","author":"Afink","year":"2008","journal-title":"J. Clin. Endocrinol. 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