{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T16:03:27Z","timestamp":1790525007626,"version":"4.1.0"},"reference-count":52,"publisher":"MDPI AG","issue":"14","license":[{"start":{"date-parts":[[2022,7,21]],"date-time":"2022-07-21T00:00:00Z","timestamp":1658361600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Agence Nationale de la Recherche to C.A.","award":["ANR-10-EMMA-042"],"award-info":[{"award-number":["ANR-10-EMMA-042"]}]},{"name":"Agence Nationale de la Recherche to C.A.","award":["ANR-20-CE14-0024-01"],"award-info":[{"award-number":["ANR-20-CE14-0024-01"]}]},{"name":"Agence Nationale de la Recherche to C.A.","award":["DEQ20180339193"],"award-info":[{"award-number":["DEQ20180339193"]}]},{"name":"Agence Nationale de la Recherche to G.L.","award":["ANR-10-EMMA-042"],"award-info":[{"award-number":["ANR-10-EMMA-042"]}]},{"name":"Agence Nationale de la Recherche to G.L.","award":["ANR-20-CE14-0024-01"],"award-info":[{"award-number":["ANR-20-CE14-0024-01"]}]},{"name":"Agence Nationale de la Recherche to G.L.","award":["DEQ20180339193"],"award-info":[{"award-number":["DEQ20180339193"]}]},{"name":"Fondation de la Recherche M\u00e9dicale","award":["ANR-10-EMMA-042"],"award-info":[{"award-number":["ANR-10-EMMA-042"]}]},{"name":"Fondation de la Recherche M\u00e9dicale","award":["ANR-20-CE14-0024-01"],"award-info":[{"award-number":["ANR-20-CE14-0024-01"]}]},{"name":"Fondation de la Recherche M\u00e9dicale","award":["DEQ20180339193"],"award-info":[{"award-number":["DEQ20180339193"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>The group X secreted phospholipase A2 (PLA2G10) is present at high levels in mouse sperm acrosome. The enzyme is secreted during capacitation and amplifies the acrosome reaction and its own secretion via an autocrine loop. PLA2G10 also improves the rate of fertilization. In in vitro fertilization (IVF) experiments, sperm from Pla2g10-deficient mice produces fewer two-cell embryos, and the absence of PLA2G10 is rescued by adding recombinant enzymes. Moreover, wild-type (WT) sperm treated with recombinant PLA2G10 produces more two-cell embryos. The effects of PLA2G10 on mouse fertility are inhibited by sPLA2 inhibitors and rescued by products of the enzymatic reaction such as free fatty acids, suggesting a role of catalytic activity. However, PLA2G10 also binds to mouse PLA2R1, which may play a role in fertility. To determine the relative contribution of enzymatic activity and PLA2R1 binding in the profertility effect of PLA2G10, we tested H48Q-PLA2G10, a catalytically-inactive mutant of PLA2G10 with low enzymatic activity but high binding properties to PLA2R1. Its effect was tested in various mouse strains, including Pla2r1-deficient mice. H48Q-PLA2G10 did not trigger the acrosome reaction but was as potent as WT-PLA2G10 to improve IVF in inbred C57Bl\/6 mice; however, this was not the case in OF1 outbred mice. Using gametes from these mouse strains, the effect of H48Q-PLA2G10 appeared dependent on both spermatozoa and oocytes. Moreover, sperm from C57Bl\/6 Pla2r1-deficient mice were less fertile and lowered the profertility effects of H48Q-PLA2G10, which were completely suppressed when sperm and oocytes were collected from Pla2r1-deficient mice. Conversely, the effect of WT-PLA2G10 was not or less sensitive to the absence of PLA2R1, suggesting that the effect of PLA2G10 is polymodal and complex, acting both as an enzyme and a ligand of PLA2R1. This study shows that the action of PLA2G10 on gametes is complex and can simultaneously activate the catalytic pathway and the PLA2R1-dependent receptor pathway. This work also shows for the first time that PLA2G10 binding to gametes\u2019 PLA2R1 participates in fertilization optimization.<\/jats:p>","DOI":"10.3390\/ijms23148033","type":"journal-article","created":{"date-parts":[[2022,7,21]],"date-time":"2022-07-21T10:36:56Z","timestamp":1658399816000},"page":"8033","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Treatment of Mouse Sperm with a Non-Catalytic Mutant of PLA2G10 Reveals That PLA2G10 Improves In Vitro Fertilization through Both Its Enzymatic Activity and as Ligand of PLA2R1"],"prefix":"10.3390","volume":"23","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8767-6462","authenticated-orcid":false,"given":"Roland","family":"Abi Nahed","sequence":"first","affiliation":[{"name":"Universit\u00e9 Grenoble Alpes, F-38000 Grenoble, France"},{"name":"Institute for Advanced Bioscience, INSERM 1209, CNRS UMR 5309, F-38700 La Tronche, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Magali","family":"Dhellemmes","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Grenoble Alpes, F-38000 Grenoble, France"},{"name":"Institute for Advanced Bioscience, INSERM 1209, CNRS UMR 5309, F-38700 La Tronche, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3640-5061","authenticated-orcid":false,"given":"Christine","family":"Payr\u00e9","sequence":"additional","affiliation":[{"name":"Institut de Pharmacologie Mol\u00e9culaire et Cellulaire, Universit\u00e9 C\u00f4te d\u2019Azur, CNRS, Valbonne Sophia Antipolis, F-06560 Valbonne, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emilie","family":"Le Bl\u00e9vec","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Grenoble Alpes, F-38000 Grenoble, France"},{"name":"Institute for Advanced Bioscience, INSERM 1209, CNRS UMR 5309, F-38700 La Tronche, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Philippe","family":"Perrier","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Grenoble Alpes, F-38000 Grenoble, France"},{"name":"Institute for Advanced Bioscience, INSERM 1209, CNRS UMR 5309, F-38700 La Tronche, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1851-6932","authenticated-orcid":false,"given":"Sylviane","family":"Hennebicq","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Grenoble Alpes, F-38000 Grenoble, France"},{"name":"Institute for Advanced Bioscience, INSERM 1209, CNRS UMR 5309, F-38700 La Tronche, France"},{"name":"CHU de Grenoble, Centre d\u2019AMP-CECOS, CS1021, F-38000 Grenoble, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jessica","family":"Escoffier","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Grenoble Alpes, F-38000 Grenoble, France"},{"name":"Institute for Advanced Bioscience, INSERM 1209, CNRS UMR 5309, F-38700 La Tronche, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pierre F.","family":"Ray","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Grenoble Alpes, F-38000 Grenoble, France"},{"name":"Institute for Advanced Bioscience, INSERM 1209, CNRS UMR 5309, F-38700 La Tronche, France"},{"name":"CHU de Grenoble, UF de Biochimie et G\u00e9n\u00e9tique Mol\u00e9culaire, F-38000 Grenoble, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6474-4453","authenticated-orcid":false,"given":"Corinne","family":"Loeuillet","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Grenoble Alpes, F-38000 Grenoble, France"},{"name":"Institute for Advanced Bioscience, INSERM 1209, CNRS UMR 5309, F-38700 La Tronche, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9239-518X","authenticated-orcid":false,"given":"G\u00e9rard","family":"Lambeau","sequence":"additional","affiliation":[{"name":"Institut de Pharmacologie Mol\u00e9culaire et Cellulaire, Universit\u00e9 C\u00f4te d\u2019Azur, CNRS, Valbonne Sophia Antipolis, F-06560 Valbonne, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christophe","family":"Arnoult","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Grenoble Alpes, F-38000 Grenoble, France"},{"name":"Institute for Advanced Bioscience, INSERM 1209, CNRS UMR 5309, F-38700 La Tronche, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,7,21]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"6130","DOI":"10.1021\/cr200085w","article-title":"Phospholipase A2 enzymes: Physical structure, biological function, disease implication, chemical inhibition, and therapeutic intervention","volume":"111","author":"Dennis","year":"2011","journal-title":"Chem. 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