{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T04:33:08Z","timestamp":1762057988092,"version":"build-2065373602"},"reference-count":40,"publisher":"MDPI AG","issue":"15","license":[{"start":{"date-parts":[[2022,7,22]],"date-time":"2022-07-22T00:00:00Z","timestamp":1658448000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"U.S. Department of Agriculture (USDA)"},{"name":"Agricultural Research Service (ARS)"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Beet curly top virus (BCTV) significantly reduces sugar beet yield in semi-arid production areas. Genetic resistance to BCTV is limited; therefore, identification of additional resistance-associated factors is highly desired. Using 16S rRNA sequencing and BCTV resistant (R) genotypes (KDH13, KDH4-9) along with a susceptible (S) genotype (KDH19-17), we investigated leaf bacteriome changes during BCTV post inoculation (pi). At day 6 (~6-week-old plants), Cyanobacteria were predominant (~90%); whereas, at week 4 (~10-week-old plants) Firmicutes (11\u201366%), Bacteroidetes (17\u201326%), and Verrucomicrobia (12\u201329%) were predominant phyla and genotype dependent. Both Bacteroidetes and Verrucomicrobia, increased post infection only in the R lines. The bacterial genera Brevibacillus increased at 6 dpi, and Akkermansia and Bacteroides at 4 wkpi in the R lines. Linear discriminant analysis effect size (LEfSe) identified potential biomarkers in the R vs. S lines. Functional profiling revealed bacterial enrichment associated with the TCA cycle, polyisoprenoid, and L-methionine biosynthesis pathways only in KDH4-9 at 6 dpi. At 4 wkpi, bacteria associated with tryptophan and palmitate biosynthesis in the R lines, and uridine monophosphate, phosphatidyl glycerol, and phospholipid biosynthesis in the S line, were enriched. Future characterization of bacterial genera with antiviral properties will help establish their use as biocontrol agents\/biomarkers against BCTV.<\/jats:p>","DOI":"10.3390\/ijms23158073","type":"journal-article","created":{"date-parts":[[2022,7,22]],"date-time":"2022-07-22T12:53:45Z","timestamp":1658494425000},"page":"8073","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Leaf Bacteriome in Sugar Beet Shows Differential Response against Beet curly top virus during Resistant and Susceptible Interactions"],"prefix":"10.3390","volume":"23","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9451-3071","authenticated-orcid":false,"given":"Rajtilak","family":"Majumdar","sequence":"first","affiliation":[{"name":"Northwest Irrigation and Soils Research, United States Department of Agriculture (USDA)\u2014Agricultural Research Service (ARS), Kimberly, ID 83341, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6773-1375","authenticated-orcid":false,"given":"Carl A.","family":"Strausbaugh","sequence":"additional","affiliation":[{"name":"Northwest Irrigation and Soils Research, United States Department of Agriculture (USDA)\u2014Agricultural Research Service (ARS), Kimberly, ID 83341, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7457-1517","authenticated-orcid":false,"given":"Eric D.","family":"Vincill","sequence":"additional","affiliation":[{"name":"Northwest Irrigation and Soils Research, United States Department of Agriculture (USDA)\u2014Agricultural Research Service (ARS), Kimberly, ID 83341, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Imad","family":"Eujayl","sequence":"additional","affiliation":[{"name":"Northwest Irrigation and Soils Research, United States Department of Agriculture (USDA)\u2014Agricultural Research Service (ARS), Kimberly, ID 83341, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paul J.","family":"Galewski","sequence":"additional","affiliation":[{"name":"Northwest Irrigation and Soils Research, United States Department of Agriculture (USDA)\u2014Agricultural Research Service (ARS), Kimberly, ID 83341, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,7,22]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"307","DOI":"10.1007\/0-306-48575-3_9","article-title":"Management of Sugarbeet Diseases","volume":"Volume 1","author":"Srivastava","year":"2004","journal-title":"Fruit and Vegetable Diseases"},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Panella, L., Kaffka, S.R., Lewellen, R.T., Mitchell McGrath, J., Metzger, M.S., and Strausbaugh, C.A. 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