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The molecular mechanisms that drive the brain-lung interaction post-stroke have not yet been elucidated. We performed transient middle cerebral artery occlusion (MCAO) and sham surgery on C57BL\/6J mice and collected bronchoalveolar lavage fluid (BALF), serum, brain, and lung homogenate samples 24 h after surgery. A 92 proteins-panel developed by Olink Proteomics\u00ae was used to analyze the content in BALF and lung homogenates. MCAO animals had higher protein concentration levels in BALF than sham-controls, but these levels did not correlate with the infarct volume. No alteration in alveolar-capillary barrier permeability was observed. A total of 12 and 14 proteins were differentially expressed between the groups (FDR &lt; 0.1) in BALF and lung tissue homogenates, respectively. Of those, HGF, TGF-\u03b1, and CCL2 were identified as the most relevant to this study. Their protein expression patterns were verified by ELISA. This study confirmed that post-stroke lung damage was not associated with increased lung permeability or cerebral ischemia severity. Furthermore, the dysregulation of HGF, TGF-\u03b1, and CCL2 in BALF and lung tissue after ischemia could play an important role in the molecular mechanisms underlying stroke-induced lung damage.<\/jats:p>","DOI":"10.3390\/ijms23158093","type":"journal-article","created":{"date-parts":[[2022,7,22]],"date-time":"2022-07-22T12:53:45Z","timestamp":1658494425000},"page":"8093","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Evaluation and Characterization of Post-Stroke Lung Damage in a Murine Model of Cerebral Ischemia"],"prefix":"10.3390","volume":"23","author":[{"given":"J\u00falia","family":"Faura","sequence":"first","affiliation":[{"name":"Neurovascular Research Laboratory, Valld\u2019Hebron Research Institute (VHIR), Universitat Aut\u00f2noma de Barcelona (UAB), 08035 Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"Ramiro","sequence":"additional","affiliation":[{"name":"Neurovascular Research Laboratory, Valld\u2019Hebron Research Institute (VHIR), Universitat Aut\u00f2noma de Barcelona (UAB), 08035 Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alba","family":"Simats","sequence":"additional","affiliation":[{"name":"Neurovascular Research Laboratory, Valld\u2019Hebron Research Institute (VHIR), Universitat Aut\u00f2noma de Barcelona (UAB), 08035 Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Feifei","family":"Ma","sequence":"additional","affiliation":[{"name":"Neurovascular Research Laboratory, Valld\u2019Hebron Research Institute (VHIR), Universitat Aut\u00f2noma de Barcelona (UAB), 08035 Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"Penalba","sequence":"additional","affiliation":[{"name":"Neurovascular Research Laboratory, Valld\u2019Hebron Research Institute (VHIR), Universitat Aut\u00f2noma de Barcelona (UAB), 08035 Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9321-1741","authenticated-orcid":false,"given":"Teresa","family":"Gasull","sequence":"additional","affiliation":[{"name":"Cellular and Molecular Neurobiology Research Group, Department of Neurosciences, Germans Trias i Pujol Research Institute (IGTP), 08916 Badalona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"Rosell","sequence":"additional","affiliation":[{"name":"Neurovascular Research Laboratory, Valld\u2019Hebron Research Institute (VHIR), Universitat Aut\u00f2noma de Barcelona (UAB), 08035 Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4845-2279","authenticated-orcid":false,"given":"Joan","family":"Montaner","sequence":"additional","affiliation":[{"name":"Neurovascular Research Laboratory, Valld\u2019Hebron Research Institute (VHIR), Universitat Aut\u00f2noma de Barcelona (UAB), 08035 Barcelona, Spain"},{"name":"Stroke Research Program, Institute of Biomedicine of Seville, IBiS\/Hospital Universitario Virgen del Roc\u00edo\/CSIC, University of Seville, 41013 Seville, Spain"},{"name":"Department of Neurology, Hospital Universitario Virgen Macarena, 41009 Seville, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alejandro","family":"Bustamante","sequence":"additional","affiliation":[{"name":"Neurovascular Research Laboratory, Valld\u2019Hebron Research Institute (VHIR), Universitat Aut\u00f2noma de Barcelona (UAB), 08035 Barcelona, Spain"},{"name":"Stroke Unit, Hospital Universitari Germans Trias i Pujol, 08916 Badalona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,7,22]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"163","DOI":"10.5492\/wjccm.v4.i3.163","article-title":"Brain-Lung Crosstalk: Implications for Neurocritical Care Patients","volume":"4","author":"Mrozek","year":"2015","journal-title":"World J. 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