{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,14]],"date-time":"2025-11-14T10:41:53Z","timestamp":1763116913261,"version":"build-2065373602"},"reference-count":27,"publisher":"MDPI AG","issue":"15","license":[{"start":{"date-parts":[[2022,7,23]],"date-time":"2022-07-23T00:00:00Z","timestamp":1658534400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001659","name":"German Research Foundation","doi-asserted-by":"publisher","award":["DFG No. GU405\/14-1","DFG, GR 1094\/6-1"],"award-info":[{"award-number":["DFG No. GU405\/14-1","DFG, GR 1094\/6-1"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Alloreactive and autoreactive antibodies have been associated with the development of chronic lung allograft dysfunction (CLAD), but their pathogenic role is disputed. Orthotopic left lung transplantation was performed in the Fischer-344 to Lewis rat strain combination followed by the application of ciclosporine for 10 days. Four weeks after transplantation, lipopolysaccharide (LPS) was instilled into the trachea. Lungs were harvested before (postoperative day 28) and after LPS application (postoperative days 29, 33, 40, and 90) for histopathological, immunohistochemical, and Western blot analyses. Recipient serum was collected to investigate circulating antibodies. Lung allografts were more strongly infiltrated by B cells and deposits of immunoglobulin G and M were more prominent in allografts compared to right native lungs or isografts and increased in response to LPS instillation. LPS induced the secretion of autoreactive antibodies into the circulation of allograft and isograft recipients, while alloreactive antibodies were only rarely detected. Infiltration of B cells and accumulation of immunoglobulin, which is observed in allografts treated with LPS but not isografts or native lungs, might contribute to the pathogenesis of experimental CLAD. However, the LPS-induced appearance of circulating autoreactive antibodies does not seem to be related to CLAD, because it is observed in both, isograft and allograft recipients.<\/jats:p>","DOI":"10.3390\/ijms23158111","type":"journal-article","created":{"date-parts":[[2022,7,24]],"date-time":"2022-07-24T22:49:02Z","timestamp":1658702942000},"page":"8111","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Activation of Humoral Immunity during the Pathogenesis of Experimental Chronic Lung Allograft Dysfunction"],"prefix":"10.3390","volume":"23","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0795-0941","authenticated-orcid":false,"given":"Martin","family":"Reichert","sequence":"first","affiliation":[{"name":"Laboratory of Experimental Surgery, Department of General and Thoracic Surgery, Excellence Cluster Cardio-Pulmonary Institute (CPI), Member of the German Center for Lung Research (DZL), Justus-Liebig-University Giessen, Feulgen-Strasse 10-12, D-35385 Giessen, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Srebrena","family":"Atanasova","sequence":"additional","affiliation":[{"name":"Laboratory of Experimental Surgery, Department of General and Thoracic Surgery, Excellence Cluster Cardio-Pulmonary Institute (CPI), Member of the German Center for Lung Research (DZL), Justus-Liebig-University Giessen, Feulgen-Strasse 10-12, D-35385 Giessen, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kathrin","family":"Petri","sequence":"additional","affiliation":[{"name":"Laboratory of Experimental Surgery, Department of General and Thoracic Surgery, Excellence Cluster Cardio-Pulmonary Institute (CPI), Member of the German Center for Lung Research (DZL), Justus-Liebig-University Giessen, Feulgen-Strasse 10-12, D-35385 Giessen, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marian","family":"Kampschulte","sequence":"additional","affiliation":[{"name":"Department of Diagnostic and Interventional Radiology, University Hospital Giessen, Member of the German Center for Lung Research (DZL), Justus-Liebig-University Giessen, Klinikstrasse 33, D-35392 Giessen, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Baktybek","family":"Kojonazarov","sequence":"additional","affiliation":[{"name":"Institute for Lung Health, Excellence Cluster Cardio-Pulmonary Institute (CPI), Member of the German Center for Lung Research (DZL), Justus-Liebig-University Giessen, Aulweg 130, D-35392 Giessen, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gabriele","family":"Fuchs-Moll","sequence":"additional","affiliation":[{"name":"Laboratory of Experimental Surgery, Department of General and Thoracic Surgery, Excellence Cluster Cardio-Pulmonary Institute (CPI), Member of the German Center for Lung Research (DZL), Justus-Liebig-University Giessen, Feulgen-Strasse 10-12, D-35385 Giessen, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gabriele A.","family":"Krombach","sequence":"additional","affiliation":[{"name":"Department of Diagnostic and Interventional Radiology, University Hospital Giessen, Member of the German Center for Lung Research (DZL), Justus-Liebig-University Giessen, Klinikstrasse 33, D-35392 Giessen, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Winfried","family":"Padberg","sequence":"additional","affiliation":[{"name":"Laboratory of Experimental Surgery, Department of General and Thoracic Surgery, Excellence Cluster Cardio-Pulmonary Institute (CPI), Member of the German Center for Lung Research (DZL), Justus-Liebig-University Giessen, Feulgen-Strasse 10-12, D-35385 Giessen, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7512-2051","authenticated-orcid":false,"given":"Veronika","family":"Grau","sequence":"additional","affiliation":[{"name":"Laboratory of Experimental Surgery, Department of General and Thoracic Surgery, Excellence Cluster Cardio-Pulmonary Institute (CPI), Member of the German Center for Lung Research (DZL), Justus-Liebig-University Giessen, Feulgen-Strasse 10-12, D-35385 Giessen, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,7,23]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"493","DOI":"10.1016\/j.healun.2019.03.009","article-title":"Chronic lung allograft dysfunction: Definition, diagnostic criteria, and approaches to treatment\u2014A consensus report from the Pulmonary Council of the ISHLT","volume":"38","author":"Verleden","year":"2019","journal-title":"J. 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Biol."}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/23\/15\/8111\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T23:55:18Z","timestamp":1760140518000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/23\/15\/8111"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,7,23]]},"references-count":27,"journal-issue":{"issue":"15","published-online":{"date-parts":[[2022,8]]}},"alternative-id":["ijms23158111"],"URL":"https:\/\/doi.org\/10.3390\/ijms23158111","relation":{},"ISSN":["1422-0067"],"issn-type":[{"type":"electronic","value":"1422-0067"}],"subject":[],"published":{"date-parts":[[2022,7,23]]}}}