{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T23:45:56Z","timestamp":1790120756000,"version":"4.0.1"},"reference-count":68,"publisher":"MDPI AG","issue":"15","license":[{"start":{"date-parts":[[2022,7,24]],"date-time":"2022-07-24T00:00:00Z","timestamp":1658620800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31802033"],"award-info":[{"award-number":["31802033"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["2018143"],"award-info":[{"award-number":["2018143"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Guangdong Provincial Promotion Project on Preservation and utilization of Local Breed of Livestock and Poultry","award":["31802033"],"award-info":[{"award-number":["31802033"]}]},{"name":"Guangdong Provincial Promotion Project on Preservation and utilization of Local Breed of Livestock and Poultry","award":["2018143"],"award-info":[{"award-number":["2018143"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Litter size is an important indicator to measure the production capacity of commercial pigs. Spontaneous embryo loss is an essential factor in determining sow litter size. In early pregnancy, spontaneous embryo loss in porcine is as high as 20\u201330% during embryo implantation. However, the specific molecular mechanism underlying spontaneous embryo loss at the end of embryo implantation remains unknown. Therefore, we comprehensively used small RNA sequencing technology, bioinformatics analysis, and molecular experiments to determine the microRNA (miRNA) expression profile in the healthy and arresting embryo implantation site of porcine endometrium on day of gestation (DG) 28. A total of 464 miRNAs were identified in arresting endometrium (AE) and healthy endometrium (HE), and 139 differentially expressed miRNAs (DEMs) were screened. We combined the mRNA sequencing dataset from the SRA database to predict the target genes of these miRNAs. A quantitative real-time PCR assay identified the expression levels of miRNAs and mRNAs. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analyses were performed on differentially expressed target genes of DEMs, mainly enriched in epithelial development and amino acids metabolism-related pathways. We performed fluorescence in situ hybridization (FISH) and the dual-luciferase report gene assay to confirm miRNA and predicted target gene binding. miR-205 may inhibit its expression by combining 3\u2032-untranslated regions (3\u2032 UTR) of tubulointerstitial nephritis antigen-like 1 (TINAGL1). The resulting inhibition of angiogenesis in the maternal endometrium ultimately leads to the formation of arresting embryos during the implantation period. This study provides a reference for the effect of miRNA on the successful implantation of pig embryos in early gestation.<\/jats:p>","DOI":"10.3390\/ijms23158157","type":"journal-article","created":{"date-parts":[[2022,7,24]],"date-time":"2022-07-24T22:49:02Z","timestamp":1658702942000},"page":"8157","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Differential MicroRNA Expression in Porcine Endometrium Related to Spontaneous Embryo Loss during Early Pregnancy"],"prefix":"10.3390","volume":"23","author":[{"given":"Shengchen","family":"Gu","sequence":"first","affiliation":[{"name":"National Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural University, Guangzhou 510642, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xupeng","family":"Zang","sequence":"additional","affiliation":[{"name":"National Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural University, Guangzhou 510642, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lei","family":"Jiang","sequence":"additional","affiliation":[{"name":"National Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural University, Guangzhou 510642, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8732-7377","authenticated-orcid":false,"given":"Ting","family":"Gu","sequence":"additional","affiliation":[{"name":"National Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural University, Guangzhou 510642, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fanming","family":"Meng","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Livestock and Poultry Breeding, Guangdong Key Laboratory of Animal Breeding and Nutrition, Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Guangzhou 510642, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sixiu","family":"Huang","sequence":"additional","affiliation":[{"name":"National Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural University, Guangzhou 510642, China"},{"name":"Guangdong Provincial Key Laboratory of Agri-Animal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, Guangzhou 510642, China"},{"name":"Lingnan Guangdong Laboratory of Modern Agriculture, Guangzhou 510642, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gengyuan","family":"Cai","sequence":"additional","affiliation":[{"name":"National Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural University, Guangzhou 510642, China"},{"name":"Guangdong Provincial Key Laboratory of Agri-Animal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, Guangzhou 510642, China"},{"name":"Lingnan Guangdong Laboratory of Modern Agriculture, Guangzhou 510642, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zicong","family":"Li","sequence":"additional","affiliation":[{"name":"National Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural University, Guangzhou 510642, China"},{"name":"Guangdong Provincial Key Laboratory of Agri-Animal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, Guangzhou 510642, China"},{"name":"Lingnan Guangdong Laboratory of Modern Agriculture, Guangzhou 510642, China"},{"name":"State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangzhou 510642, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhenfang","family":"Wu","sequence":"additional","affiliation":[{"name":"National Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural University, Guangzhou 510642, China"},{"name":"Guangdong Provincial Key Laboratory of Agri-Animal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, Guangzhou 510642, China"},{"name":"Lingnan Guangdong Laboratory of Modern Agriculture, Guangzhou 510642, China"},{"name":"State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangzhou 510642, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6554-7918","authenticated-orcid":false,"given":"Linjun","family":"Hong","sequence":"additional","affiliation":[{"name":"National Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural University, Guangzhou 510642, China"},{"name":"Guangdong Provincial Key Laboratory of Agri-Animal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, Guangzhou 510642, China"},{"name":"Lingnan Guangdong Laboratory of Modern Agriculture, Guangzhou 510642, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,7,24]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Zhang, X., Huang, L., Wu, T., Feng, Y., Ding, Y., Ye, P., and Yin, Z. (2015). Transcriptomic Analysis of Ovaries from Pigs with High And Low Litter Size. PLoS ONE, 10.","DOI":"10.1371\/journal.pone.0139514"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1330","DOI":"10.3390\/genes6041330","article-title":"Transcriptomic Analysis of the Porcine Endometrium during Embryo Implantation","volume":"6","author":"Lin","year":"2015","journal-title":"Genes"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1016\/j.theriogenology.2015.08.001","article-title":"Placentation, maternal-fetal interface, and conceptus loss in swine","volume":"85","author":"Kridli","year":"2016","journal-title":"Theriogenology"},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Zang, X., Gu, T., Wang, W., Zhou, C., Ding, Y., Gu, S., Xu, Z., Xie, Y., Li, Z., and Cai, G. (2021). Integrated Insight into the Molecular Mechanisms of Spontaneous Abortion during Early Pregnancy in Pigs. Int. J. Mol. Sci., 22.","DOI":"10.3390\/ijms22126644"},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Yang, K., Wang, J., Wang, K., Luo, Y., Tang, Q., Liu, X., and Fang, M. (2020). Integrated Analysis of miRNA-mRNA Network Reveals Different Regulatory Patterns in the Endometrium of Meishan and Duroc Sows during Mid-Late Gestation. Animals, 10.","DOI":"10.3390\/ani10030420"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"470","DOI":"10.1111\/j.1600-0897.2007.00534.x","article-title":"A review of molecular contrasts between arresting and viable porcine attachment sites","volume":"58","author":"Wessels","year":"2007","journal-title":"Am. J. Reprod. Immunol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1186\/1477-7827-12-91","article-title":"Expression of leptin and its long form receptor at the porcine maternal-fetal interface: Contrasting healthy and arresting conceptus attachment sites during early and mid-pregnancy","volume":"12","author":"Kerr","year":"2014","journal-title":"Reprod. Biol. Endocrinol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"148","DOI":"10.4049\/jimmunol.176.1.148","article-title":"Differential gene expression in endometrium, endometrial lymphocytes, and trophoblasts during successful and abortive embryo implantation","volume":"176","author":"Tayade","year":"2006","journal-title":"J. Immunol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"856","DOI":"10.1002\/mrd.22847","article-title":"Pregnancy and spontaneous fetal loss: A pig perspective","volume":"84","author":"Bidarimath","year":"2017","journal-title":"Mol. Reprod. Dev."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"415","DOI":"10.1105\/tpc.111.094144","article-title":"Effective small RNA destruction by the expression of a short tandem target mimic in Arabidopsis","volume":"24","author":"Yan","year":"2012","journal-title":"Plant Cell"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"94","DOI":"10.1038\/nrg2504","article-title":"Small silencing RNAs: An expanding universe","volume":"10","author":"Ghildiyal","year":"2009","journal-title":"Nat. Rev. Genet."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"64","DOI":"10.1016\/j.jri.2014.11.004","article-title":"Distinct microRNA expression in endometrial lymphocytes, endometrium, and trophoblast during spontaneous porcine fetal loss","volume":"107","author":"Bidarimath","year":"2015","journal-title":"J. Reprod. Immunol."},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Su, L., Liu, R., Cheng, W., Zhu, M., Li, X., Zhao, S., and Yu, M. (2014). Expression patterns of microRNAs in porcine endometrium and their potential roles in embryo implantation and placentation. PLoS ONE, 9.","DOI":"10.1371\/journal.pone.0087867"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1095\/biolreprod.114.123588","article-title":"MicroRNAome of porcine conceptuses and trophoblasts: Expression profile of micrornas and their potential to regulate genes crucial for establishment of pregnancy","volume":"92","author":"Krawczynski","year":"2015","journal-title":"Biol. Reprod."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1186\/s12958-016-0152-2","article-title":"MicroRNA-21 and PDCD4 expression during in vitro oocyte maturation in pigs","volume":"14","author":"Wright","year":"2016","journal-title":"Reprod. Biol. Endocrinol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"224","DOI":"10.1016\/j.bbrc.2016.09.046","article-title":"PPARgamma is regulated by miR-27b-3p negatively and plays an important role in porcine oocyte maturation","volume":"479","author":"Song","year":"2016","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1476","DOI":"10.1016\/j.theriogenology.2016.01.004","article-title":"BDNF-induced expansion of cumulus-oocyte complexes in pigs was mediated by microRNA-205","volume":"85","author":"Li","year":"2016","journal-title":"Theriogenology"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"417","DOI":"10.1002\/mrd.22975","article-title":"Impact of preimplantational oral low-dose estradiol-17beta exposure on the endometrium: The role of miRNA","volume":"85","author":"Floter","year":"2018","journal-title":"Mol. Reprod. Dev."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Kaczmarek, M.M., Najmula, J., Guzewska, M.M., and Przygrodzka, E. (2020). MiRNAs in the Peri-Implantation Period: Contribution to Embryo-Maternal Communication in Pigs. Int. J. Mol. Sci., 21.","DOI":"10.3390\/ijms21062229"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1095\/biolreprod.111.096669","article-title":"Small RNA profile of the cumulus-oocyte complex and early embryos in the pig","volume":"87","author":"Yang","year":"2012","journal-title":"Biol. Reprod."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"914","DOI":"10.1002\/mrd.22823","article-title":"MicroRNA regulation of immune events at conception","volume":"84","author":"Robertson","year":"2017","journal-title":"Mol. Reprod. Dev."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1567","DOI":"10.1017\/S1751731113001183","article-title":"MicroRNAs in farm animals","volume":"7","author":"Wang","year":"2013","journal-title":"Animal"},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Krawczynski, K., Bauersachs, S., Reliszko, Z.P., Graf, A., and Kaczmarek, M.M. (2015). Expression of microRNAs and isomiRs in the porcine endometrium: Implications for gene regulation at the maternal-conceptus interface. BMC Genom., 16.","DOI":"10.1186\/s12864-015-2172-2"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1016\/j.ygeno.2016.11.007","article-title":"Identification of non-coding and coding RNAs in porcine endometrium","volume":"109","author":"Wang","year":"2017","journal-title":"Genomics"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"674924","DOI":"10.3389\/fphys.2021.674924","article-title":"GLUT4 in Mouse Endometrial Epithelium: Roles in Embryonic Development and Implantation","volume":"12","author":"Long","year":"2021","journal-title":"Front. Physiol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"718","DOI":"10.1095\/biolreprod66.3.718","article-title":"Analysis of osteopontin at the maternal-placental interface in pigs","volume":"66","author":"Garlow","year":"2002","journal-title":"Biol. Reprod."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"183","DOI":"10.4142\/jvs.2017.18.2.183","article-title":"Porcine circovirus type 2 increases interleukin-1beta and interleukin-10 production via the MyD88-NF-kappa B signaling pathway in porcine alveolar macrophages in vitro","volume":"18","author":"Han","year":"2017","journal-title":"J. Vet. Sci."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"E433","DOI":"10.1210\/jc.2014-3720","article-title":"Decreased Notch pathway signaling in the endometrium of women with endometriosis impairs decidualization","volume":"100","author":"Su","year":"2015","journal-title":"J. Clin. Endocrinol. Metab."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1868","DOI":"10.1038\/s41598-021-81189-5","article-title":"Differential expression and correlation analysis of miRNA-mRNA profiles in swine testicular cells infected with porcine epidemic diarrhea virus","volume":"11","author":"Zhang","year":"2021","journal-title":"Sci. Rep."},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Chakladar, J., Wong, L.M., Kuo, S.Z., Li, W.T., Yu, M.A., Chang, E.Y., Wang, X.Q., and Ongkeko, W.M. (2020). The Liver Microbiome Is Implicated in Cancer Prognosis and Modulated by Alcohol and Hepatitis B. Cancers, 12.","DOI":"10.3390\/cancers12061642"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"919","DOI":"10.5713\/ajas.2011.11033","article-title":"Activated Leukocyte Cell Adhesion Molecule: Expression in the Uterine Endometrium during the Estrous Cycle and Pregnancy in Pigs","volume":"24","author":"Kim","year":"2011","journal-title":"Asian-Australas. J. Anim. Sci."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"229","DOI":"10.1016\/j.repbio.2013.07.001","article-title":"Transcriptomic analysis of the porcine endometrium during early pregnancy and the estrous cycle","volume":"13","author":"Franczak","year":"2013","journal-title":"Reprod. Biol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"119","DOI":"10.3892\/ol.2020.12380","article-title":"miR-21-5p promotes cell proliferation by targeting BCL11B in Thp-1 cells","volume":"21","author":"Zhang","year":"2021","journal-title":"Oncol. Lett."},{"key":"ref_34","first-page":"4655","article-title":"miR-21-5p induces cell proliferation by targeting TGFBI in non-small cell lung cancer cells","volume":"16","author":"Yan","year":"2018","journal-title":"Exp. Ther. Med."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1186\/s13075-020-2145-y","article-title":"MiR-21-5p regulates extracellular matrix degradation and angiogenesis in TMJOA by targeting Spry1","volume":"22","author":"Ma","year":"2020","journal-title":"Arthritis Res. Ther."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"576","DOI":"10.1038\/s41419-021-03803-8","article-title":"Cancer-secreted exosomal miR-21-5p induces angiogenesis and vascular permeability by targeting KRIT1","volume":"12","author":"He","year":"2021","journal-title":"Cell Death Dis."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"1327","DOI":"10.3389\/fimmu.2017.01327","article-title":"miR-148a-3p Mediates Notch Signaling to Promote the Differentiation and M1 Activation of Macrophages","volume":"8","author":"Huang","year":"2017","journal-title":"Front. Immunol."},{"key":"ref_38","first-page":"866","article-title":"MiR-143-3p suppresses the progression of ovarian cancer","volume":"10","author":"Shi","year":"2018","journal-title":"Am. J. Transl. Res."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"6303","DOI":"10.2147\/CMAR.S242179","article-title":"miR-143-3p Targets lncRNA PSMG3-AS1 to Inhibit the Proliferation of Hepatocellular Carcinoma Cells","volume":"12","author":"Zhang","year":"2020","journal-title":"Cancer Manag. Res."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"356","DOI":"10.3892\/mmr.2021.11995","article-title":"miR1433p inhibits endometriotic stromal cell proliferation and invasion by inactivating autophagy in endometriosis","volume":"23","author":"Yang","year":"2021","journal-title":"Mol. Med. Rep."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"606","DOI":"10.1038\/s41598-017-18739-3","article-title":"miR-143-3p inhibits the proliferation, migration and invasion in osteosarcoma by targeting FOSL2","volume":"8","author":"Sun","year":"2018","journal-title":"Sci. Rep."},{"key":"ref_42","first-page":"6666160","article-title":"Analysis of lncRNA, miRNA, and mRNA Expression Profiling in Type I IFN and Type II IFN Overexpressed in Porcine Alveolar Macrophages","volume":"2021","author":"Li","year":"2021","journal-title":"Int. J. Genom."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"760","DOI":"10.1002\/mrd.22813","article-title":"Rapid conceptus elongation in the pig: An interleukin 1 beta 2 and estrogen-regulated phenomenon","volume":"84","author":"Geisert","year":"2017","journal-title":"Mol. Reprod. Dev."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"837","DOI":"10.1016\/S0925-4773(03)00066-2","article-title":"Critical role for Tbx6 in mesoderm specification in the mouse embryo","volume":"120","author":"Chapman","year":"2003","journal-title":"Mech. Dev."},{"key":"ref_45","doi-asserted-by":"crossref","unstructured":"Hadjantonakis, A.K., Pisano, E., and Papaioannou, V.E. (2008). Tbx6 regulates left\/right patterning in mouse embryos through effects on nodal cilia and perinodal signaling. PLoS ONE, 3.","DOI":"10.1371\/journal.pone.0002511"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"593","DOI":"10.1038\/ncb1722","article-title":"The miR-200 family and miR-205 regulate epithelial to mesenchymal transition by targeting ZEB1 and SIP1","volume":"10","author":"Gregory","year":"2008","journal-title":"Nat. Cell Biol."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1186\/s12014-017-9144-2","article-title":"Tubulointerstitial nephritis antigen-like 1 protein is downregulated in the placenta of pre-eclamptic women","volume":"14","author":"Mary","year":"2017","journal-title":"Clin. Proteom."},{"key":"ref_48","doi-asserted-by":"crossref","unstructured":"Brown, L.J., Alawoki, M., Crawford, M.E., Reida, T., Sears, A., Torma, T., and Albig, A.R. (2010). Lipocalin-7 is a matricellular regulator of angiogenesis. PLoS ONE, 5.","DOI":"10.1371\/journal.pone.0013905"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"948","DOI":"10.1095\/biolreprod.109.078162","article-title":"Tubulointerstitial nephritis antigen-like 1 is expressed in extraembryonic tissues and interacts with laminin 1 in the Reichert membrane at postimplantation in the mouse","volume":"81","author":"Igarashi","year":"2009","journal-title":"Biol. Reprod."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1095\/biolreprod.109.080028","article-title":"Tubulointerstitial nephritis antigen-like 1 is expressed in the uterus and binds with integrins in decidualized endometrium during postimplantation in mice","volume":"82","author":"Tajiri","year":"2010","journal-title":"Biol. Reprod."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"259","DOI":"10.1017\/S0967199412000469","article-title":"Distribution of tubulointerstitial nephritis antigen-like 1 and structural matrix proteins in mouse embryos during preimplantation development in vivo and in vitro","volume":"22","author":"Sakurai","year":"2014","journal-title":"Zygote"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"334","DOI":"10.1016\/j.theriogenology.2014.07.046","article-title":"Iberian pig early pregnancy: Vascular endothelial growth factor receptor system expression in the maternofetal interface in healthy and arresting conceptuses","volume":"83","author":"Flores","year":"2015","journal-title":"Theriogenology"},{"key":"ref_53","doi-asserted-by":"crossref","unstructured":"Zang, X., Zhou, C., Wang, W., Gan, J., Li, Y., Liu, D., Liu, G., and Hong, L. (2021). Differential MicroRNA Expression Involved in Endometrial Receptivity of Goats. Biomolecules, 11.","DOI":"10.3390\/biom11030472"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"R25","DOI":"10.1186\/gb-2009-10-3-r25","article-title":"Ultrafast and memory-efficient alignment of short DNA sequences to the human genome","volume":"10","author":"Langmead","year":"2009","journal-title":"Genome Biol."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1093\/nar\/gkr688","article-title":"miRDeep2 accurately identifies known and hundreds of novel microRNA genes in seven animal clades","volume":"40","author":"Friedlander","year":"2012","journal-title":"Nucleic Acids Res."},{"key":"ref_56","doi-asserted-by":"crossref","unstructured":"Wen, M., Shen, Y., Shi, S., and Tang, T. (2012). miREvo: An integrative microRNA evolutionary analysis platform for next-generation sequencing experiments. BMC Bioinform., 13.","DOI":"10.1186\/1471-2105-13-140"},{"key":"ref_57","doi-asserted-by":"crossref","unstructured":"Zhou, L., Chen, J., Li, Z., Li, X., Hu, X., Huang, Y., Zhao, X., Liang, C., Wang, Y., and Sun, L. (2010). Integrated profiling of microRNAs and mRNAs: microRNAs located on Xq27.3 associate with clear cell renal cell carcinoma. PLoS ONE, 5.","DOI":"10.1371\/journal.pone.0015224"},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"550","DOI":"10.1186\/s13059-014-0550-8","article-title":"Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2","volume":"15","author":"Love","year":"2014","journal-title":"Genome Biol."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"2013","DOI":"10.1214\/aos\/1074290335","article-title":"The positive false discovery rate: A Bayesian interpretation and the q-value","volume":"31","author":"Storey","year":"2003","journal-title":"Ann. Stat."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"811","DOI":"10.1002\/sim.4780090710","article-title":"More powerful procedures for multiple significance testing","volume":"9","author":"Hochberg","year":"1990","journal-title":"Stat. Med."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"R1","DOI":"10.1186\/gb-2003-5-1-r1","article-title":"MicroRNA targets in Drosophila","volume":"5","author":"Enright","year":"2003","journal-title":"Genome Biol."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"W451","DOI":"10.1093\/nar\/gkl243","article-title":"RNAhybrid: microRNA target prediction easy, fast and flexible","volume":"34","author":"Kruger","year":"2006","journal-title":"Nucleic Acids Res."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"2498","DOI":"10.1101\/gr.1239303","article-title":"Cytoscape: A software environment for integrated models of biomolecular interaction networks","volume":"13","author":"Shannon","year":"2003","journal-title":"Genome Res."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"D480","DOI":"10.1093\/nar\/gkm882","article-title":"KEGG for linking genomes to life and the environment","volume":"36","author":"Kanehisa","year":"2008","journal-title":"Nucleic Acids Res."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"627","DOI":"10.1007\/s10529-011-0813-3","article-title":"PolyA RT-PCR-based quantification of microRNA by using universal TaqMan probe","volume":"34","author":"Luo","year":"2012","journal-title":"Biotechnol. Lett."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1186\/s12929-020-00668-5","article-title":"ZNF322A-mediated protein phosphorylation induces autophagosome formation through modulation of IRS1-AKT glucose uptake and HSP-elicited UPR in lung cancer","volume":"27","author":"Cheung","year":"2020","journal-title":"J. Biomed. Sci."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"943","DOI":"10.1007\/s11033-018-4242-4","article-title":"Identification of endogenous microRNA references in porcine serum for quantitative real-time PCR normalization","volume":"45","author":"Bae","year":"2018","journal-title":"Mol. Biol. Rep."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"2545","DOI":"10.1007\/s10815-020-01901-8","article-title":"Assessing and validating housekeeping genes in normal, cancerous, and polycystic human ovaries","volume":"37","author":"Asiabi","year":"2020","journal-title":"J. Assist. Reprod. Genet."}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/23\/15\/8157\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T23:55:47Z","timestamp":1760140547000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/23\/15\/8157"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,7,24]]},"references-count":68,"journal-issue":{"issue":"15","published-online":{"date-parts":[[2022,8]]}},"alternative-id":["ijms23158157"],"URL":"https:\/\/doi.org\/10.3390\/ijms23158157","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,7,24]]}}}