{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,28]],"date-time":"2026-03-28T06:23:54Z","timestamp":1774679034787,"version":"3.50.1"},"reference-count":32,"publisher":"MDPI AG","issue":"15","license":[{"start":{"date-parts":[[2022,7,27]],"date-time":"2022-07-27T00:00:00Z","timestamp":1658880000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000002","name":"NIH grants","doi-asserted-by":"publisher","award":["T32 CA078586"],"award-info":[{"award-number":["T32 CA078586"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"NIH grants","doi-asserted-by":"publisher","award":["P01 CA217797"],"award-info":[{"award-number":["P01 CA217797"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"NIH grants","doi-asserted-by":"publisher","award":["G-17-1500"],"award-info":[{"award-number":["G-17-1500"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"NIH grants","doi-asserted-by":"publisher","award":["NIH P30 CA086862"],"award-info":[{"award-number":["NIH P30 CA086862"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100001634","name":"Gateway for Cancer Research","doi-asserted-by":"publisher","award":["T32 CA078586"],"award-info":[{"award-number":["T32 CA078586"]}],"id":[{"id":"10.13039\/100001634","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100001634","name":"Gateway for Cancer Research","doi-asserted-by":"publisher","award":["P01 CA217797"],"award-info":[{"award-number":["P01 CA217797"]}],"id":[{"id":"10.13039\/100001634","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100001634","name":"Gateway for Cancer Research","doi-asserted-by":"publisher","award":["G-17-1500"],"award-info":[{"award-number":["G-17-1500"]}],"id":[{"id":"10.13039\/100001634","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100001634","name":"Gateway for Cancer Research","doi-asserted-by":"publisher","award":["NIH P30 CA086862"],"award-info":[{"award-number":["NIH P30 CA086862"]}],"id":[{"id":"10.13039\/100001634","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Carver College of Medicine","award":["T32 CA078586"],"award-info":[{"award-number":["T32 CA078586"]}]},{"name":"Carver College of Medicine","award":["P01 CA217797"],"award-info":[{"award-number":["P01 CA217797"]}]},{"name":"Carver College of Medicine","award":["G-17-1500"],"award-info":[{"award-number":["G-17-1500"]}]},{"name":"Carver College of Medicine","award":["NIH P30 CA086862"],"award-info":[{"award-number":["NIH P30 CA086862"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Polyoxometalate nanoparticles (POMs) are a class of compounds made up of multiple transition metals linked together using oxygen atoms. POMs commonly include group 6 transition metals, with two of the most common forms using molybdenum and tungsten. POMs are suggested to exhibit antimicrobial effects. In this study, we developed two POM preparations to study anti-cancer activity. We found that Mo-POM (NH4)Mo7O24) and W-POM (H3PW12O40) have anti-cancer effects on glioblastoma cells. Both POMs induced morphological changes marked by membrane swelling and the presence of multinucleated cells that may indicate apoptosis induction along with impaired cell division. We also observed significant increases in lipid oxidation events, suggesting that POMs are redox-active and can catalyze detrimental oxidation events in glioblastoma cells. Here, we present preliminary indications that molybdenum polyoxometalate nanoparticles may act like ferrous iron to catalyze the oxidation of phospholipids. These preliminary results suggest that Mo-POMs (NH4)Mo7O24) and W-POMs (H3PW12O40) may warrant further investigation into their utility as adjunct cancer therapies.<\/jats:p>","DOI":"10.3390\/ijms23158263","type":"journal-article","created":{"date-parts":[[2022,7,27]],"date-time":"2022-07-27T21:11:05Z","timestamp":1658956265000},"page":"8263","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Polyoxometalate Nanoparticles as a Potential Glioblastoma Therapeutic via Lipid-Mediated Cell Death"],"prefix":"10.3390","volume":"23","author":[{"given":"Michael S.","family":"Petronek","sequence":"first","affiliation":[{"name":"Department of Radiation Oncology, Division of Free Radical and Radiation Biology, The University of Iowa, Iowa City, IA 52242-1181, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bryan G.","family":"Allen","sequence":"additional","affiliation":[{"name":"Spheres4Life B.V., 7521 Enschede, The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gregor","family":"Luthe","sequence":"additional","affiliation":[{"name":"Spheres4Life B.V., 7521 Enschede, The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jeffrey M.","family":"Stolwijk","sequence":"additional","affiliation":[{"name":"Department of Radiation Oncology, Division of Free Radical and Radiation Biology, The University of Iowa, Iowa City, IA 52242-1181, USA"},{"name":"Spheres4Life B.V., 7521 Enschede, The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,7,27]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"0112","DOI":"10.1038\/s41570-018-0112","article-title":"Synthesis, structures and applications of electron-rich polyoxometalates","volume":"2","author":"Gumerova","year":"2018","journal-title":"Nat. Rev. Chem."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1652","DOI":"10.1039\/D0EE03407J","article-title":"Polyoxometalates (POMs): From electroactive clusters to energy materials","volume":"14","author":"Horn","year":"2021","journal-title":"Energy Environ. Sci."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"2757","DOI":"10.1021\/cr950061t","article-title":"The Mononuclear Molybdenum Enzymes","volume":"96","author":"Hille","year":"1996","journal-title":"Chem. Rev."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1016\/j.acthis.2003.08.001","article-title":"Comparative histochemical and immunohistochemical study on xanthine oxidoreductase\/xanthine oxidase in mammalian corneal epithelium","volume":"106","author":"Ardan","year":"2004","journal-title":"Acta Histochem."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1111\/j.1432-1033.1989.tb14993.x","article-title":"Carboxylic acid reductase: A new tungsten enzyme catalyses the reduction of non-activated carboxylic acids to aldehydes","volume":"184","author":"White","year":"1989","journal-title":"Eur. J. Biochem."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1153","DOI":"10.1039\/C7CC07549A","article-title":"The antibacterial activity of polyoxometalates: Structures, antibiotic effects and future perspectives","volume":"54","author":"Bijelic","year":"2018","journal-title":"Chem. Commun."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"362","DOI":"10.2174\/0929867326666190827153532","article-title":"Polyoxometalates in Biomedicine: Update and Overview","volume":"27","author":"Mougharbel","year":"2020","journal-title":"Curr. Med. Chem."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"327","DOI":"10.1021\/cr960396q","article-title":"Polyoxometalates in Medicine","volume":"98","author":"Rhule","year":"1998","journal-title":"Chem. Rev."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"33529","DOI":"10.1038\/srep33529","article-title":"Degradable Organically-Derivatized Polyoxometalate with Enhanced Activity against Glioblastoma Cell Line","volume":"6","author":"She","year":"2016","journal-title":"Sci. Rep."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1016\/j.freeradbiomed.2022.02.023","article-title":"Sodium molybdate inhibits the growth of ovarian cancer cells via inducing both ferroptosis and apoptosis","volume":"182","author":"Mao","year":"2022","journal-title":"Free. Radic. Biol. Med."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"2980","DOI":"10.1002\/anie.201803868","article-title":"Polyoxometalates as Potential Next-Generation Metallodrugs in the Combat against Cancer","volume":"58","author":"Bijelic","year":"2019","journal-title":"Angew. Chem. Int. Ed."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"535","DOI":"10.1006\/abbi.1993.1074","article-title":"The Pecking Order of Free Radicals and Antioxidants: Lipid Peroxidation, \u03b1-Tocopherol, and Ascorbate","volume":"300","author":"Buettner","year":"1993","journal-title":"Arch. Biochem. Biophys."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"413","DOI":"10.1021\/tx00034a003","article-title":"Polyunsaturated fatty acid alkoxyl radicals exist as carbon-centered epoxyallylic radicals: A key step in hydroperoxide-amplified lipid peroxidation","volume":"6","author":"Wilcox","year":"1993","journal-title":"Chem. Res. Toxicol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1042\/bss0610065","article-title":"The chemistry of lipid alkoxyl radicals and their role in metal-amplified lipid peroxidation","volume":"61","author":"Marnett","year":"1995","journal-title":"Biochem. Soc. Symp."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"568","DOI":"10.1016\/S0891-5849(00)00407-X","article-title":"EPR detection of lipid-derived free radicals from PUFA, LDL, and cell oxidations","volume":"29","author":"Qian","year":"2000","journal-title":"Free. Radic. Biol. Med."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"146282","DOI":"10.1155\/2015\/146282","article-title":"Targeting the Mitotic Catastrophe Signaling Pathway in Cancer","volume":"2015","year":"2015","journal-title":"Mediat. Inflamm."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"528","DOI":"10.1016\/S0008-6363(99)00384-3","article-title":"Morphologic and biochemical hallmarks of apoptosis","volume":"45","author":"Saraste","year":"2000","journal-title":"Cardiovasc. Res."},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Miller, M., and Zachary, J. (2017). Mechanisms and Morphology of Cellular Injury, Adaptation, and Death. Pathol. Basis Vet. Dis., 2\u201343.e19.","DOI":"10.1016\/B978-0-323-35775-3.00001-1"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"427","DOI":"10.3389\/fmicb.2012.00427","article-title":"Redox chemistry of molybdenum in natural waters and its involvement in biological evolution","volume":"3","author":"Wang","year":"2012","journal-title":"Front. Microbiol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1060","DOI":"10.1016\/j.cell.2012.03.042","article-title":"Ferroptosis: An Iron-Dependent Form of Nonapoptotic Cell Death","volume":"149","author":"Dixon","year":"2012","journal-title":"Cell"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"14506","DOI":"10.1073\/pnas.1516869112","article-title":"Electrochemical evidence that pyranopterin redox chemistry controls the catalysis of YedY, a mononuclear Mo enzyme","volume":"112","author":"Adamson","year":"2015","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1016\/0003-9861(89)90261-0","article-title":"Studies of ascorbate-dependent, iron-catalyzed lipid peroxidation","volume":"271","author":"Miller","year":"1989","journal-title":"Arch. Biochem. Biophys."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1016\/j.atherosclerosis.2004.01.040","article-title":"Iron-catalyzed lipid peroxidation in aortic cells in vitro: Protective effect of extracellular magnesium","volume":"175","author":"Kostellow","year":"2004","journal-title":"Atherosclerosis"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"13165","DOI":"10.1074\/jbc.R113.455311","article-title":"The Molybdenum Cofactor*","volume":"288","author":"Mendel","year":"2013","journal-title":"J. Biol. Chem."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"8838","DOI":"10.1021\/acs.inorgchem.1c00763","article-title":"Synthesis, Structure, and Spectroscopic Study of Redox-Active Heterometallic Cluster-Based Complexes [Re5MoSe8(CN)6]n","volume":"60","author":"Muravieva","year":"2021","journal-title":"Inorg. Chem."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1186\/s13014-022-02052-z","article-title":"Integrative analysis of therapy resistance and transcriptomic profiling data in glioblastoma cells identifies sensitization vulnerabilities for combined modality radiochemotherapy","volume":"17","author":"Albrecht","year":"2022","journal-title":"Radiat. Oncol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1016\/j.cbi.2014.10.012","article-title":"Polyoxometalates as antitumor agents: Bioactivity of a new polyoxometalate with copper on a human osteosarcoma model","volume":"222","author":"Leon","year":"2014","journal-title":"Chem.-Biol. Interact."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"1300","DOI":"10.1128\/AAC.32.9.1300","article-title":"Clinical trial of tolerance of HPA-23 in patients with acquired immune deficiency syndrome","volume":"32","author":"Moskovitz","year":"1988","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"451","DOI":"10.1016\/0753-3322(89)90245-X","article-title":"Toxicity of HPA-23 (am-monmm-21-tungsto-9-antimoniate) for normal human myeloid progenitor cells (GM-CFU) in vitro","volume":"43","author":"Aymard","year":"1989","journal-title":"Biomed. Pharmacother."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"14766","DOI":"10.1038\/s41598-021-94302-5","article-title":"Differences between antioxidant defense parameters and specific trace element concentrations in healthy, benign, and malignant brain tissues","volume":"11","year":"2021","journal-title":"Sci. Rep."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"473","DOI":"10.1016\/S0891-5849(02)00848-1","article-title":"C11-BODIPY581\/591, an oxidation-sensitive fluorescent lipid peroxidation probe: (micro) spectroscopic characterization and validation of methodology","volume":"33","author":"Drummen","year":"2002","journal-title":"Free. Radic. Biol. Med."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"4449","DOI":"10.1021\/bi00181a003","article-title":"Free Radical-Mediated Lipid Peroxidation in Cells: Oxidizability Is a Function of Cell Lipid bis-Allylic Hydrogen Content","volume":"33","author":"Wagner","year":"1994","journal-title":"Biochemistry"}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/23\/15\/8263\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T23:57:10Z","timestamp":1760140630000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/23\/15\/8263"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,7,27]]},"references-count":32,"journal-issue":{"issue":"15","published-online":{"date-parts":[[2022,8]]}},"alternative-id":["ijms23158263"],"URL":"https:\/\/doi.org\/10.3390\/ijms23158263","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,7,27]]}}}