{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T01:59:55Z","timestamp":1787795995405,"version":"build-2784847793"},"reference-count":30,"publisher":"MDPI AG","issue":"15","license":[{"start":{"date-parts":[[2022,7,30]],"date-time":"2022-07-30T00:00:00Z","timestamp":1659139200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"EU ERANET-Neuron","award":["ANR-16-NEU3-003"],"award-info":[{"award-number":["ANR-16-NEU3-003"]}]},{"name":"EU ERANET-Neuron","award":["WFL-FR-07\/17-N\u00b0 154"],"award-info":[{"award-number":["WFL-FR-07\/17-N\u00b0 154"]}]},{"name":"EU ERANET-Neuron","award":["ANR-10-IBHU-0006"],"award-info":[{"award-number":["ANR-10-IBHU-0006"]}]},{"name":"Wings for Life Spinal Cord Research Foundation","award":["ANR-16-NEU3-003"],"award-info":[{"award-number":["ANR-16-NEU3-003"]}]},{"name":"Wings for Life Spinal Cord Research Foundation","award":["WFL-FR-07\/17-N\u00b0 154"],"award-info":[{"award-number":["WFL-FR-07\/17-N\u00b0 154"]}]},{"name":"Wings for Life Spinal Cord Research Foundation","award":["ANR-10-IBHU-0006"],"award-info":[{"award-number":["ANR-10-IBHU-0006"]}]},{"name":"French Agence Nationale pour la Recherche (ANR)","award":["ANR-16-NEU3-003"],"award-info":[{"award-number":["ANR-16-NEU3-003"]}]},{"name":"French Agence Nationale pour la Recherche (ANR)","award":["WFL-FR-07\/17-N\u00b0 154"],"award-info":[{"award-number":["WFL-FR-07\/17-N\u00b0 154"]}]},{"name":"French Agence Nationale pour la Recherche (ANR)","award":["ANR-10-IBHU-0006"],"award-info":[{"award-number":["ANR-10-IBHU-0006"]}]},{"name":"French Society of Physical and Rehabilitation Medicine (SOFMER)","award":["ANR-16-NEU3-003"],"award-info":[{"award-number":["ANR-16-NEU3-003"]}]},{"name":"French Society of Physical and Rehabilitation Medicine (SOFMER)","award":["WFL-FR-07\/17-N\u00b0 154"],"award-info":[{"award-number":["WFL-FR-07\/17-N\u00b0 154"]}]},{"name":"French Society of Physical and Rehabilitation Medicine (SOFMER)","award":["ANR-10-IBHU-0006"],"award-info":[{"award-number":["ANR-10-IBHU-0006"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Background: Neurogenic detrusor overactivity (NDO) is a severe pathological condition characterized by involuntary detrusor contractions leading to urine leakage. This condition is frequent after spinal cord injury (SCI). Gene therapy for NDO requires the development of vectors that express therapeutic transgenes driven by sensory neuron-specific promoters. The aim of this study was to develop and assess tools for the characterization of sensory neuron-specific promoters in dorsal root ganglia (DRG) neurons after transduction with herpes simplex virus type 1 (HSV-1)-based amplicon defective vectors. Methods: The HSV-1 vector genome encoded two independent transcription cassettes: one expressed firefly luciferase (FLuc) driven by different promoters\u2019 candidates (rTRPV1, rASIC3, rCGRP, or hCGRP), and the other expressed a reporter gene driven by an invariable promoter. The strength and selectivity of promoters was assessed in organotypic cultures of explanted adult DRG, or sympathetic and parasympathetic ganglia from control and SCI rats. Results: The rCGRP promoter induced selective expression in the DRG of normal rats. The rTRPV-1 promoter, which did not display selective activity in control rats, induced selective expression in DRG explanted from SCI rats. Conclusions: This study provides a methodology to assess sensory neuron-specific promoters, opening new perspectives for future gene therapy for NDO.<\/jats:p>","DOI":"10.3390\/ijms23158474","type":"journal-article","created":{"date-parts":[[2022,8,1]],"date-time":"2022-08-01T02:06:42Z","timestamp":1659319602000},"page":"8474","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Development and Assessment of Herpes Simplex Virus Type 1 (HSV-1) Amplicon Vectors with Sensory Neuron-Selective Promoters"],"prefix":"10.3390","volume":"23","author":[{"given":"Charles","family":"Joussain","sequence":"first","affiliation":[{"name":"UMR INSERM U1179\u2014Universit\u00e9 de Versailles Saint Quentin en Yvelines\/Paris Saclay, UFR des Sciences de la Sant\u00e9 Simone Veil, 2, Avenue de la Source de la Bi\u00e8vre, 78180 Montigny-le-Bretonneux, France"},{"name":"Neuro-Urology R. Poincar\u00e9 Hospital AP-HP, 104 bvd R. Poincar\u00e9, 92380 Garches, France"},{"name":"Ipsen Innovation SAS, 5 Avenue du Canada, Zone Industrielle de Courtaboeuf, 91940 Les Ulis, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olivier","family":"Le Coz","sequence":"additional","affiliation":[{"name":"UMR INSERM U1179\u2014Universit\u00e9 de Versailles Saint Quentin en Yvelines\/Paris Saclay, UFR des Sciences de la Sant\u00e9 Simone Veil, 2, Avenue de la Source de la Bi\u00e8vre, 78180 Montigny-le-Bretonneux, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrey","family":"Pichugin","sequence":"additional","affiliation":[{"name":"UMR INSERM U1179\u2014Universit\u00e9 de Versailles Saint Quentin en Yvelines\/Paris Saclay, UFR des Sciences de la Sant\u00e9 Simone Veil, 2, Avenue de la Source de la Bi\u00e8vre, 78180 Montigny-le-Bretonneux, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3488-0491","authenticated-orcid":false,"given":"Peggy","family":"Marconi","sequence":"additional","affiliation":[{"name":"Department of Chemical, Pharmaceutical and Agricultural Sciences (DOCPAS), Via Luigi Borsari, 44121 Ferrara, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6570-1017","authenticated-orcid":false,"given":"Filip","family":"Lim","sequence":"additional","affiliation":[{"name":"Centro de Biologia Molecular Severo Ochoa, Universidad Autonoma de Madrid (UAM), CSIC-UAM, Calle Nicolas Cabrera 1, Cantoblanco, 28049 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariaconcetta","family":"Sicurella","sequence":"additional","affiliation":[{"name":"Department of Chemical, Pharmaceutical and Agricultural Sciences (DOCPAS), Via Luigi Borsari, 44121 Ferrara, Italy"},{"name":"IRCCS Ospedale San Raffaele, Urological Research Institute, Via Olgettina 60, 20132 Milan, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keith","family":"Foster","sequence":"additional","affiliation":[{"name":"Ipsen Bioinnovation Ltd., 102 Park Drive, Milton Park, Abingdon OX14 4RY, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fran\u00e7ois","family":"Giuliano","sequence":"additional","affiliation":[{"name":"UMR INSERM U1179\u2014Universit\u00e9 de Versailles Saint Quentin en Yvelines\/Paris Saclay, UFR des Sciences de la Sant\u00e9 Simone Veil, 2, Avenue de la Source de la Bi\u00e8vre, 78180 Montigny-le-Bretonneux, France"},{"name":"Neuro-Urology R. Poincar\u00e9 Hospital AP-HP, 104 bvd R. Poincar\u00e9, 92380 Garches, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alberto L.","family":"Epstein","sequence":"additional","affiliation":[{"name":"UMR INSERM U1179\u2014Universit\u00e9 de Versailles Saint Quentin en Yvelines\/Paris Saclay, UFR des Sciences de la Sant\u00e9 Simone Veil, 2, Avenue de la Source de la Bi\u00e8vre, 78180 Montigny-le-Bretonneux, France"},{"name":"EG427, P\u00e9pini\u00e8re H\u00f4pital Cochin, 29 Rue du Faubourg Saint-Jacques, 75014 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alejandro","family":"Aranda Mu\u00f1oz","sequence":"additional","affiliation":[{"name":"UMR INSERM U1179\u2014Universit\u00e9 de Versailles Saint Quentin en Yvelines\/Paris Saclay, UFR des Sciences de la Sant\u00e9 Simone Veil, 2, Avenue de la Source de la Bi\u00e8vre, 78180 Montigny-le-Bretonneux, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,7,30]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"309","DOI":"10.2147\/CLEP.S68889","article-title":"Global prevalence and incidence of traumatic spinal cord injury","volume":"6","author":"Fehlings","year":"2014","journal-title":"Clin. Epidemiol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1371","DOI":"10.1089\/neu.2004.21.1371","article-title":"Targeting Recovery: Priorities of the Spinal Cord-Injured Population","volume":"21","author":"Anderson","year":"2004","journal-title":"J. Neurotrauma"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"146","DOI":"10.1159\/000353274","article-title":"Systematic Review of the Epidemiology of Urinary Incontinence and Detrusor Overactivity among Patients with Neurogenic Overactive Bladder","volume":"41","author":"Ruffion","year":"2013","journal-title":"Neuroepidemiology"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"S21","DOI":"10.1002\/nau.22631","article-title":"Chapter 4: Guidelines for the diagnosis and treatment of overactive bladder (OAB) and neurogenic detrusor overactivity (NDO)","volume":"33","author":"Nambiar","year":"2014","journal-title":"Neurourol. Urodyn."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"2131","DOI":"10.1016\/j.juro.2012.01.125","article-title":"Phase 3 Efficacy and Tolerability Study of OnabotulinumtoxinA for Urinary Incontinence from Neurogenic Detrusor Overactivity","volume":"187","author":"Ginsberg","year":"2012","journal-title":"J. Urol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"769","DOI":"10.1016\/j.juro.2018.10.012","article-title":"Long-Term Discontinuation of Botulinum Toxin a Intradetrusor Injections for Neurogenic Detrusor Overactivity: A Multicenter Study","volume":"201","author":"Baron","year":"2018","journal-title":"J. Urol."},{"key":"ref_7","first-page":"327","article-title":"Neural Control of the Lower Urinary Tract","volume":"5","author":"Griffiths","year":"2014","journal-title":"Compr. Physiol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"365","DOI":"10.1002\/nau.22730","article-title":"Electrical nerve stimulation to promote micturition in spinal cord injury patients: A review of current attempts","volume":"35","author":"Ren","year":"2015","journal-title":"Neurourol. Urodyn."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1002\/ana.22446","article-title":"Gene therapy for pain: Results of a phase I clinical trial","volume":"70","author":"Fink","year":"2011","journal-title":"Ann. Neurol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"190","DOI":"10.1016\/j.neuroscience.2017.09.024","article-title":"Morphological changes in different populations of bladder afferent neurons detected by herpes simplex virus (HSV) vectors with cell-type-specific promoters in mice with spinal cord injury","volume":"364","author":"Shimizu","year":"2017","journal-title":"Neuroscience"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1007\/978-3-540-79090-7_4","article-title":"Afferent Nerve Regulation of Bladder Function in Health and Disease","volume":"194","author":"Yoshimura","year":"2009","journal-title":"Sens. Nerves"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"F1130","DOI":"10.1152\/ajprenal.00618.2009","article-title":"Expression and response of acid-sensing ion channels in urinary bladder to cyclophosphamide-induced cystitis","volume":"298","author":"Corrow","year":"2010","journal-title":"Am. J. Physiol. Physiol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"6","DOI":"10.1016\/j.neulet.2015.01.074","article-title":"Distribution of TRPV1 and TRPV2 in the human stellate ganglion and spinal cord","volume":"590","author":"Kokubun","year":"2015","journal-title":"Neurosci. Lett."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"e16","DOI":"10.4081\/ejh.2013.e16","article-title":"Localization of peripheral autonomic neurons innervating the boar urinary bladder trigone and neurochemical features of the sympathetic component","volume":"57","author":"Ragionieri","year":"2013","journal-title":"Eur. J. Histochem."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"10240","DOI":"10.1073\/pnas.95.17.10240","article-title":"A sensory neuron-specific, proton-gated ion channel","volume":"95","author":"Chen","year":"1998","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"596","DOI":"10.1002\/cne.20794","article-title":"Distinct expression of TRPM8, TRPA1, and TRPV1 mRNAs in rat primary afferent neurons with a\u03b4\/c-fibers and colocalization with trk receptors","volume":"493","author":"Kobayashi","year":"2005","journal-title":"J. Comp. Neurol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1261","DOI":"10.1113\/jphysiol.2011.221788","article-title":"Acid-sensing ion channel subtype 3 function and immunolabelling increases in skeletal muscle sensory neurons following femoral artery occlusion","volume":"590","author":"Xing","year":"2012","journal-title":"J. Physiol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"48907","DOI":"10.1074\/jbc.M309468200","article-title":"How Nerve Growth Factor Drives Physiological and Inflammatory Expressions of Acid-sensing Ion Channel 3 in Sensory Neurons","volume":"278","author":"Mamet","year":"2003","journal-title":"J. Biol. Chem."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"112","DOI":"10.1007\/BF02284790","article-title":"Neuropeptides and neurotransmitter-synthesizing enzymes in intrinsic neurons of the human urinary bladder","volume":"25","author":"Smet","year":"1996","journal-title":"J. Neurocytol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"85","DOI":"10.3109\/00016489309128081","article-title":"Latent Herpes Simplex Virus Type 1 in Human Vestibular Ganglia","volume":"113","author":"Furuta","year":"1993","journal-title":"Acta Oto-Laryngol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"8383","DOI":"10.1128\/JVI.00468-15","article-title":"Herpes Simplex Virus 1 Reactivates from Autonomic Ciliary Ganglia Independently from Sensory Trigeminal Ganglia to Cause Recurrent Ocular Disease","volume":"89","author":"Lee","year":"2015","journal-title":"J. Virol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"440","DOI":"10.1038\/sj.gt.3300621","article-title":"Selective astrocytic transgene expression in vitro and in vivo from the GFAP promoter in a HSV RL1 null mutant vector\u2014Potential glioblastoma targeting","volume":"5","author":"McKie","year":"1998","journal-title":"Gene Ther."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"956","DOI":"10.1002\/jgm.1519","article-title":"Novel tumour-specific promoters for transcriptional targeting of hepatocellular carcinoma by herpes simplex virus vectors","volume":"12","author":"Foka","year":"2010","journal-title":"J. Gene Med."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1016\/S0166-0934(99)00118-4","article-title":"Detection of varicella-zoster virus and herpes simplex virus by the polymerase chain reaction with degenerate primers","volume":"83","author":"Jacobs","year":"1999","journal-title":"J. Virol. Methods"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1177\/0023677217724823","article-title":"PREPARE: Guidelines for planning animal research and testing","volume":"52","author":"Smith","year":"2017","journal-title":"Lab. Anim."},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Joussain, C., Le Coz, O., Pichugin, A., Marconi, P., Lim, F., Sicurella, M., Salonia, A., Montorsi, F., Wandosell, F., and Foster, K. (2019). Botulinum Neurotoxin Light Chains Expressed by Defective Herpes Simplex Virus Type-1 Vectors Cleave SNARE Proteins and Inhibit CGRP Release in Rat Sensory Neurons. Toxins, 11.","DOI":"10.3390\/toxins11020123"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1054","DOI":"10.1016\/j.eururo.2012.01.051","article-title":"Minimal Effective dose of Dysport and Botox in a Rat Model of Neurogenic Detrusor Overactivity","volume":"61","author":"Oger","year":"2012","journal-title":"Eur. Urol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"114","DOI":"10.1111\/j.1464-410X.2009.08647.x","article-title":"Neurotrophic effects of brain-derived neurotrophic factor and vascular endothelial growth factor in major pelvic ganglia of young and aged rats","volume":"105","author":"Lin","year":"2009","journal-title":"Br. J. Urol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1049","DOI":"10.1089\/104303403322124774","article-title":"Improved Packaging System for Generation of High-Level Noncytotoxic HSV-1 Amplicon Vectors Using Cre-loxP Site-Specific Recombination to Delete the Packaging Signals of Defective Helper Genomes","volume":"14","author":"Zaupa","year":"2003","journal-title":"Hum. Gene Ther."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1593","DOI":"10.1038\/sj.gt.3300766","article-title":"Deletion of multiple immediate\u2013Early genes from herpes simplex virus reduces cytotoxicity and permits long-term gene expression in neurons","volume":"5","author":"Krisky","year":"1998","journal-title":"Gene Ther."}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/23\/15\/8474\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T00:00:03Z","timestamp":1760140803000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/23\/15\/8474"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,7,30]]},"references-count":30,"journal-issue":{"issue":"15","published-online":{"date-parts":[[2022,8]]}},"alternative-id":["ijms23158474"],"URL":"https:\/\/doi.org\/10.3390\/ijms23158474","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,7,30]]}}}