{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T03:21:37Z","timestamp":1777605697503,"version":"3.51.4"},"reference-count":48,"publisher":"MDPI AG","issue":"15","license":[{"start":{"date-parts":[[2022,8,3]],"date-time":"2022-08-03T00:00:00Z","timestamp":1659484800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81871201"],"award-info":[{"award-number":["81871201"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["JSSCRC2021543"],"award-info":[{"award-number":["JSSCRC2021543"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["KYCX21_3102"],"award-info":[{"award-number":["KYCX21_3102"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["KFJN2249"],"award-info":[{"award-number":["KFJN2249"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Jiangsu Innovation and Entrepreneurship Talent Plan to X.H.Z.","award":["81871201"],"award-info":[{"award-number":["81871201"]}]},{"name":"Jiangsu Innovation and Entrepreneurship Talent Plan to X.H.Z.","award":["JSSCRC2021543"],"award-info":[{"award-number":["JSSCRC2021543"]}]},{"name":"Jiangsu Innovation and Entrepreneurship Talent Plan to X.H.Z.","award":["KYCX21_3102"],"award-info":[{"award-number":["KYCX21_3102"]}]},{"name":"Jiangsu Innovation and Entrepreneurship Talent Plan to X.H.Z.","award":["KFJN2249"],"award-info":[{"award-number":["KFJN2249"]}]},{"name":"Postgraduate Research &amp; Practice Innovation Program of Jiangsu Province","award":["81871201"],"award-info":[{"award-number":["81871201"]}]},{"name":"Postgraduate Research &amp; Practice Innovation Program of Jiangsu Province","award":["JSSCRC2021543"],"award-info":[{"award-number":["JSSCRC2021543"]}]},{"name":"Postgraduate Research &amp; Practice Innovation Program of Jiangsu Province","award":["KYCX21_3102"],"award-info":[{"award-number":["KYCX21_3102"]}]},{"name":"Postgraduate Research &amp; Practice Innovation Program of Jiangsu Province","award":["KFJN2249"],"award-info":[{"award-number":["KFJN2249"]}]},{"name":"Large Instruments Open Foundation of Nantong University","award":["81871201"],"award-info":[{"award-number":["81871201"]}]},{"name":"Large Instruments Open Foundation of Nantong University","award":["JSSCRC2021543"],"award-info":[{"award-number":["JSSCRC2021543"]}]},{"name":"Large Instruments Open Foundation of Nantong University","award":["KYCX21_3102"],"award-info":[{"award-number":["KYCX21_3102"]}]},{"name":"Large Instruments Open Foundation of Nantong University","award":["KFJN2249"],"award-info":[{"award-number":["KFJN2249"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Many testis-specific lncRNAs are highly expressed in late spermatogenesis, especially in spermiogenesis. However, their functions and the underlying mechanisms in male fertility are largely unknown. Here, we screened two highly expressed lncRNAs, 1700101O22Rik (O22Rik) and NONMMUG030480.1 (NM480) in testes, to investigate the roles in spermatogenesis using lncRNA knockout (KO) mouse generated by CRISPER\/Cas9 technology. Both testis-specific lncRNAs were mainly expressed from secondary spermatocytes to round spermatids, suggesting that they might be involved in spermiogenesis. Phenotypic analysis showed that the deletion of O22Rik or NM480 did not affect the development of testis and epididymis or spermatogenesis. These results were confirmed in both young and middle-aged male mice. In addition, there was no significant difference in sperm morphology and other parameters including concentration and motility between wild type (WT) and KO mice. Fertility tests showed that litter size was significantly lower in O22Rik KO mice compared with WT controls. Although O22Rik did not exert dramatic roles in spermatogenesis, on molecular levels, its surrounding gene expression was disturbed significantly. Gm32773 was decreased; however, Gm32828 was increased in KO mice. In conclusion, lncRNA O22Rik and NM480 are not individually essential for spermatogenesis in mice.<\/jats:p>","DOI":"10.3390\/ijms23158627","type":"journal-article","created":{"date-parts":[[2022,8,3]],"date-time":"2022-08-03T20:52:01Z","timestamp":1659559921000},"page":"8627","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["lncRNA 1700101O22Rik and NONMMUG030480.1 Are Not Essential for Spermatogenesis in Mice"],"prefix":"10.3390","volume":"23","author":[{"given":"Yang","family":"Zhou","sequence":"first","affiliation":[{"name":"Institute of Reproductive Medicine, Medical School, Nantong University, Nantong 226019, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shijue","family":"Dong","sequence":"additional","affiliation":[{"name":"Institute of Reproductive Medicine, Medical School, Nantong University, Nantong 226019, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chen","family":"Chen","sequence":"additional","affiliation":[{"name":"Institute of Reproductive Medicine, Medical School, Nantong University, Nantong 226019, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaojun","family":"Liu","sequence":"additional","affiliation":[{"name":"Institute of Reproductive Medicine, Medical School, Nantong University, Nantong 226019, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xuhui","family":"Zeng","sequence":"additional","affiliation":[{"name":"Institute of Reproductive Medicine, Medical School, Nantong University, Nantong 226019, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuan","family":"Gao","sequence":"additional","affiliation":[{"name":"Experimental Animal Center, Nantong University, Nantong 226001, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1227-866X","authenticated-orcid":false,"given":"Xiaoning","family":"Zhang","sequence":"additional","affiliation":[{"name":"Institute of Reproductive Medicine, Medical School, Nantong University, Nantong 226019, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,8,3]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1152\/physrev.00013.2015","article-title":"Spermatogenesis: The Commitment to Meiosis","volume":"96","author":"Griswold","year":"2016","journal-title":"Physiol. 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