{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,21]],"date-time":"2026-07-21T03:19:12Z","timestamp":1784603952575,"version":"3.55.0"},"reference-count":100,"publisher":"MDPI AG","issue":"15","license":[{"start":{"date-parts":[[2022,8,3]],"date-time":"2022-08-03T00:00:00Z","timestamp":1659484800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Center for Research and Development","award":["POWR.03.02.00\u201300-I029\/16"],"award-info":[{"award-number":["POWR.03.02.00\u201300-I029\/16"]}]},{"name":"National Center for Research and Development","award":["503\/1-013-02\/503-11-001-19-00"],"award-info":[{"award-number":["503\/1-013-02\/503-11-001-19-00"]}]},{"name":"Medical University of Lodz","award":["POWR.03.02.00\u201300-I029\/16"],"award-info":[{"award-number":["POWR.03.02.00\u201300-I029\/16"]}]},{"name":"Medical University of Lodz","award":["503\/1-013-02\/503-11-001-19-00"],"award-info":[{"award-number":["503\/1-013-02\/503-11-001-19-00"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>One of the main treatment modalities for non-small-cell lung cancer (NSCLC) is cisplatin-based chemotherapy. However, the acquisition of cisplatin resistance remains a major problem. Existing chemotherapy regimens are often ineffective against cancer cells expressing aldehyde dehydrogenase (ALDH). As such, there is an urgent need for therapies targeting ALDH-positive cancer cells. The present study compares the anticancer properties of 36 structurally diverse isothiocyanates (ITCs) against NSCLC cells with the ALDH inhibitor disulfiram (DSF). Their potential affinity to ALDH isoforms and ABC proteins was assessed using AutoDockTools, allowing for selection of three compounds presenting the strongest affinity to all tested proteins. The selected ITCs had no impact on NSCLC cell viability (at tested concentrations), but significantly decreased the cisplatin tolerance of cisplatin-resistant variant of A549 (A549CisR) and advanced (stage 4) NSCLC cell line H1581. Furthermore, long-term supplementation with ITC 1-(isothiocyanatomethyl)-4-phenylbenzene reverses the EMT phenotype and migratory potential of A549CisR to the level presented by parental A549 cells, increasing E-Cadherin expression, followed by decreased expression of ABCC1 and ALDH3A1. Our data indicates that the ALDH inhibitors DSF and ITCs are potential adjuvants of cisplatin chemotherapy.<\/jats:p>","DOI":"10.3390\/ijms23158644","type":"journal-article","created":{"date-parts":[[2022,8,3]],"date-time":"2022-08-03T20:52:01Z","timestamp":1659559921000},"page":"8644","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Isothiocyanates (ITCs) 1-(Isothiocyanatomethyl)-4-phenylbenzene and 1-Isothiocyanato-3,5-bis(trifluoromethyl)benzene\u2014Aldehyde Dehydrogenase (ALDH) Inhibitors, Decreases Cisplatin Tolerance and Migratory Ability of NSCLC"],"prefix":"10.3390","volume":"23","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7342-7342","authenticated-orcid":false,"given":"Jolanta","family":"Kryczka","sequence":"first","affiliation":[{"name":"Department of Biomedicine and Genetics, Medical University of Lodz, 92-213 Lodz, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5719-4139","authenticated-orcid":false,"given":"Jakub","family":"Kryczka","sequence":"additional","affiliation":[{"name":"Institute of Medical Biology, Polish Academy of Sciences, 93-232 Lodz, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4689-1622","authenticated-orcid":false,"given":"\u0141ukasz","family":"Janczewski","sequence":"additional","affiliation":[{"name":"Institute of Organic Chemistry, Faculty of Chemistry, Lodz University of Technology, 90-924 Lodz, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"Gajda","sequence":"additional","affiliation":[{"name":"Institute of Organic Chemistry, Faculty of Chemistry, Lodz University of Technology, 90-924 Lodz, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8847-1808","authenticated-orcid":false,"given":"Andrzej","family":"Fr\u0105czyk","sequence":"additional","affiliation":[{"name":"Institute of Applied Computer Science, Lodz University of Technology, 90-537 Lodz, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8419-7012","authenticated-orcid":false,"given":"Joanna","family":"Boncela","sequence":"additional","affiliation":[{"name":"Institute of Medical Biology, Polish Academy of Sciences, 93-232 Lodz, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4581-947X","authenticated-orcid":false,"given":"Beata","family":"Kolesi\u0144ska","sequence":"additional","affiliation":[{"name":"Institute of Organic Chemistry, Faculty of Chemistry, Lodz University of Technology, 90-924 Lodz, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0882-1458","authenticated-orcid":false,"given":"Ewa","family":"Brzezia\u0144ska-Lasota","sequence":"additional","affiliation":[{"name":"Department of Biomedicine and Genetics, Medical University of Lodz, 92-213 Lodz, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,8,3]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Kryczka, J., Migdalska-S\u0119k, M., Kordiak, J., Kisza\u0142kiewicz, J.M., Pastuszak-Lewandoska, D., Antczak, A., and Brzezia\u0144ska-Lasota, E. 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