{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,6]],"date-time":"2026-01-06T13:10:36Z","timestamp":1767705036318,"version":"build-2065373602"},"reference-count":27,"publisher":"MDPI AG","issue":"15","license":[{"start":{"date-parts":[[2022,8,5]],"date-time":"2022-08-05T00:00:00Z","timestamp":1659657600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004329","name":"Slovenian Research Agency","doi-asserted-by":"publisher","award":["P3-0323","P3-0054","P3-0108","IP-0510"],"award-info":[{"award-number":["P3-0323","P3-0054","P3-0108","IP-0510"]}],"id":[{"id":"10.13039\/501100004329","id-type":"DOI","asserted-by":"publisher"}]},{"name":"MRIC UL","award":["P3-0323","P3-0054","P3-0108","IP-0510"],"award-info":[{"award-number":["P3-0323","P3-0054","P3-0108","IP-0510"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Therapy with mesenchymal stem cells (MSCs) is promising in many diseases. Evaluation of their efficacy depends on adequate follow-up of MSCs after transplantation. Several studies have shown that MSCs can be labeled and subsequently visualized with magnetic nanoparticles (NPs). We investigated the homing of MSCs labeled with magnetic cobalt ferrite NPs in experimentally induced acute kidney injury in mice. To explore the homing of MSCs after systemic infusion into mice, we developed a pre-infusion strategy for optimal tracing and identification of MSCs with polyacrylic acid-coated cobalt ferrite (CoFe2O4) NPs by light and transmission electron microscopy (TEM) in various organs of mice with cisplatin-induced acute kidney injury and control mice. By correlative microscopy, we detected MSCs labeled with NPs in the lungs, spleen, kidney, and intestine of cisplatin-treated mice and in the lungs and spleen of control mice. Our results confirm that labeling MSCs with metal NPs did not affect the ultrastructure of MSCs and their ability to settle in various organs. This study demonstrates the usefulness of cobalt ferrite NPs in ex vivo visualization of MSCs and offers correlative microscopy as a useful method in routine histopathology laboratories for tracing MSCs in paraffin-embedded tissue.<\/jats:p>","DOI":"10.3390\/ijms23158738","type":"journal-article","created":{"date-parts":[[2022,8,7]],"date-time":"2022-08-07T22:51:46Z","timestamp":1659912706000},"page":"8738","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Cobalt Ferrite Magnetic Nanoparticles for Tracing Mesenchymal Stem Cells in Tissue: A Preliminary Study"],"prefix":"10.3390","volume":"23","author":[{"given":"\u017deljka","family":"Ve\u010deri\u0107-Haler","sequence":"first","affiliation":[{"name":"Department of Nephrology, University Medical Center Ljubljana, 1000 Ljubljana, Slovenia"},{"name":"Faculty of Medicine, University of Ljubljana, 1000 Ljubljana, Slovenia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1893-4349","authenticated-orcid":false,"given":"Nika","family":"Kojc","sequence":"additional","affiliation":[{"name":"Institute of Pathology, Faculty of Medicine, University of Ljubljana, 1000 Ljubljana, Slovenia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0011-6460","authenticated-orcid":false,"given":"Karmen","family":"Wechtersbach","sequence":"additional","affiliation":[{"name":"Institute of Pathology, Faculty of Medicine, University of Ljubljana, 1000 Ljubljana, Slovenia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9397-9366","authenticated-orcid":false,"given":"Martina","family":"Per\u0161e","sequence":"additional","affiliation":[{"name":"Medical Experimental Centre, Faculty of Medicine, University of Ljubljana, 1000 Ljubljana, Slovenia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andreja","family":"Erman","sequence":"additional","affiliation":[{"name":"Institute of Cell Biology, Faculty of Medicine, University of Ljubljana, 1000 Ljubljana, Slovenia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,8,5]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"301","DOI":"10.1111\/j.1582-4934.2004.tb00320.x","article-title":"Adult mesenchymal stem cells: Characterization, differentiation, and application in cell and gene therapy","volume":"8","author":"Baksh","year":"2004","journal-title":"J. Cell. Mol. Med."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1016\/j.arcmed.2020.08.006","article-title":"Mesenchymal Stem Cells Current Clinical Applications: A Systematic Review","volume":"52","author":"Caplan","year":"2021","journal-title":"Arch. Med. Res."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1007\/978-1-4939-3584-0_7","article-title":"Paracrine Mechanisms of Mesenchymal Stem Cells in Tissue Repair","volume":"1416","author":"Gnecchi","year":"2016","journal-title":"Methods Mol. Biol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1038\/s41536-019-0083-6","article-title":"Mesenchymal stem cell perspective: Cell biology to clinical progress","volume":"4","author":"Pittenger","year":"2019","journal-title":"NPJ Regen. Med."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"801","DOI":"10.1177\/0963689719837897","article-title":"The Pros and Cons of Mesenchymal Stem Cell-Based Therapies","volume":"28","author":"Kot","year":"2019","journal-title":"Cell Transpl."},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Accomasso, L., Gallina, C., Turinetto, V., and Cell, C.G. (2016). Tracking with Nanoparticles for Regenerative Medicine Purposes: An Overview. Stem Cells Int., 2016.","DOI":"10.1155\/2016\/7920358"},{"key":"ref_7","first-page":"1","article-title":"Iron Oxide Nanoparticles in Mesenchymal Stem Cell Detection and Therapy","volume":"1","author":"Mehta","year":"2022","journal-title":"Stem Cell Rev. Rep."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"12417","DOI":"10.1002\/chem.200901548","article-title":"Durable mesenchymal stem cell labelling by using polyhedral superparamagnetic iron oxide nanoparticles","volume":"15","author":"Wang","year":"2009","journal-title":"Chemistry"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"3875","DOI":"10.2147\/IJN.S205574","article-title":"A brief review of cytotoxicity of nanoparticles on mesenchymal stem cells in regenerative medicine","volume":"14","author":"Liu","year":"2019","journal-title":"Int. J. Nanomed."},{"key":"ref_10","first-page":"919","article-title":"Visualization of internalization of functionalized cobalt ferrite nanoparticles and their intracellular fate","volume":"8","author":"Bregar","year":"2013","journal-title":"Int. J. Nanomed."},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Perse, M., and Veceric-Haler, Z. (2018). Cisplatin-Induced Rodent Model of Kidney Injury: Characteristics and Challenges. Biomed. Res. Int., 2018.","DOI":"10.1155\/2018\/1462802"},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Veceric-Haler, Z., Erman, A., Cerar, A., Motaln, H., Kolo\u0161a, K., Turn\u0161ek, T.L., \u0160emrl, S.S., Lakota, K., Mrak-Polj\u0161ak, K., and \u0160krajnar, \u0160. (2016). Improved Protective Effect of Umbilical Cord Stem Cell Transplantation on Cisplatin-Induced Kidney Injury in Mice Pretreated with Antithymocyte Globulin. Stem Cells Int., 2016.","DOI":"10.1155\/2016\/3585362"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1186\/s13287-019-1296-8","article-title":"Labeling of human mesenchymal stem cells with different classes of vital stains: Robustness and toxicity","volume":"10","author":"Andrzejewska","year":"2019","journal-title":"Stem Cell Res. Ther."},{"key":"ref_14","first-page":"686","article-title":"Labeling stem cells with fluorescent dyes for non-invasive detection with optical imaging","volume":"2","author":"Boddington","year":"2008","journal-title":"J. Vis. Exp."},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Farinha, P., Coelho, J.M.P., Reis, C.P., and Gaspar, M.M. (2021). A Comprehensive Updated Review on Magnetic Nanoparticles in Diagnostics. Nanomaterials, 11.","DOI":"10.3390\/nano11123432"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"544","DOI":"10.7150\/thno.5634","article-title":"Commercial nanoparticles for stem cell labeling and tracking","volume":"3","author":"Wang","year":"2013","journal-title":"Theranostics"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"421","DOI":"10.1016\/j.isci.2019.05.004","article-title":"Mesenchymal Stromal Cell Homing: Mechanisms and Strategies for Improvement","volume":"15","author":"Ullah","year":"2019","journal-title":"iScience"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"650664","DOI":"10.3389\/fcell.2021.650664","article-title":"The Current Status of Mesenchymal Stromal Cells: Controversies, Unresolved Issues and Some Promising Solutions to Improve Their Therapeutic Efficacy","volume":"9","author":"Bueren","year":"2021","journal-title":"Front. Cell Dev. Biol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"315","DOI":"10.1080\/14653240600855905","article-title":"Minimal criteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statement","volume":"8","author":"Dominici","year":"2006","journal-title":"Cytotherapy"},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Pawelczyk, E., Jordan, E.K., Balakumaran, A., Chaudhry, A., Gormley, N., Smith, M., Lewis, B.K., Childs, R., Robey, P.G., and Frank, J.A. (2009). In vivo transfer of intracellular labels from locally implanted bone marrow stromal cells to resident tissue macrophages. PLoS ONE, 4.","DOI":"10.1371\/journal.pone.0006712"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"616","DOI":"10.1148\/radiol.2017162759","article-title":"Ten Things You Might Not Know about Iron Oxide Nanoparticles","volume":"284","year":"2017","journal-title":"Radiology"},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Rajan, A., Sharma, M., and Sahu, N.K. (2020). Assessing magnetic and inductive thermal properties of various surfactants functionalised Fe3O4 nanoparticles for hyperthermia. Sci. Rep., 10.","DOI":"10.1038\/s41598-020-71703-6"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"169","DOI":"10.4274\/tjps.99609","article-title":"In Vitro Evaluation of the Toxicity of Cobalt Ferrite Nanoparticles in Kidney Cell","volume":"14","author":"Abudayyak","year":"2017","journal-title":"Turk. J. Pharm. Sci."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"492","DOI":"10.1021\/acs.chemrestox.6b00377","article-title":"Pitfalls and Challenges in Nanotoxicology: A Case of Cobalt Ferrite (CoFe2O4) Nanocomposites","volume":"30","author":"Ahmad","year":"2017","journal-title":"Chem. Res. Toxicol."},{"key":"ref_25","first-page":"1449","article-title":"Cell type-specific response to high intracellular loading of polyacrylic acid-coated magnetic nanoparticles","volume":"10","author":"Lojk","year":"2015","journal-title":"Int. J. Nanomed."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1007\/s00418-017-1605-1","article-title":"Increased endocytosis of magnetic nanoparticles into cancerous urothelial cells versus normal urothelial cells","volume":"149","author":"Lojk","year":"2018","journal-title":"Histochem. Cell Biol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1330","DOI":"10.1634\/stemcells.2004-0013","article-title":"Mesenchymal stem cells in the Wharton\u2019s jelly of the human umbilical cord","volume":"22","author":"Wang","year":"2004","journal-title":"Stem Cells"}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/23\/15\/8738\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T00:04:47Z","timestamp":1760141087000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/23\/15\/8738"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,8,5]]},"references-count":27,"journal-issue":{"issue":"15","published-online":{"date-parts":[[2022,8]]}},"alternative-id":["ijms23158738"],"URL":"https:\/\/doi.org\/10.3390\/ijms23158738","relation":{},"ISSN":["1422-0067"],"issn-type":[{"type":"electronic","value":"1422-0067"}],"subject":[],"published":{"date-parts":[[2022,8,5]]}}}