{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T09:42:50Z","timestamp":1780393370897,"version":"3.54.1"},"reference-count":72,"publisher":"MDPI AG","issue":"15","license":[{"start":{"date-parts":[[2022,8,6]],"date-time":"2022-08-06T00:00:00Z","timestamp":1659744000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Ume\u00e5 University (Medical Faculty Strategic Research Resources grants, Forskarlyftet and Insamlingsstiftelsen)","award":["RV-769441"],"award-info":[{"award-number":["RV-769441"]}]},{"name":"Ume\u00e5 University (Medical Faculty Strategic Research Resources grants, Forskarlyftet and Insamlingsstiftelsen)","award":["2017-02130"],"award-info":[{"award-number":["2017-02130"]}]},{"name":"Ume\u00e5 University","award":["RV-769441"],"award-info":[{"award-number":["RV-769441"]}]},{"name":"Ume\u00e5 University","award":["2017-02130"],"award-info":[{"award-number":["2017-02130"]}]},{"name":"Vinnova","award":["RV-769441"],"award-info":[{"award-number":["RV-769441"]}]},{"name":"Vinnova","award":["2017-02130"],"award-info":[{"award-number":["2017-02130"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Injuries to large peripheral nerves are often associated with tissue defects and require reconstruction using autologous nerve grafts, which have limited availability and result in donor site morbidity. Peripheral nerve-derived hydrogels could potentially supplement or even replace these grafts. In this study, three decellularization protocols based on the ionic detergents sodium dodecyl sulfate (P1) and sodium deoxycholate (P2), or the organic solvent tri-n-butyl phosphate (P3), were used to prepare hydrogels. All protocols resulted in significantly decreased amounts of genomic DNA, but the P2 hydrogel showed the best preservation of extracellular matrix proteins, cytokines, and chemokines, and reduced levels of sulfated glycosaminoglycans. In vitro P1 and P2 hydrogels supported Schwann cell viability, secretion of VEGF, and neurite outgrowth. Surgical repair of a 10 mm-long rat sciatic nerve gap was performed by implantation of tubular polycaprolactone conduits filled with hydrogels followed by analyses using diffusion tensor imaging and immunostaining for neuronal and glial markers. The results demonstrated that the P2 hydrogel considerably increased the number of axons and the distance of regeneration into the distal nerve stump. In summary, the method used to decellularize nerve tissue affects the efficacy of the resulting hydrogels to support regeneration after nerve injury.<\/jats:p>","DOI":"10.3390\/ijms23158746","type":"journal-article","created":{"date-parts":[[2022,8,7]],"date-time":"2022-08-07T22:51:46Z","timestamp":1659912706000},"page":"8746","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Efficacy of Nerve-Derived Hydrogels to Promote Axon Regeneration Is Influenced by the Method of Tissue Decellularization"],"prefix":"10.3390","volume":"23","author":[{"given":"Vijay Kumar","family":"Kuna","sequence":"first","affiliation":[{"name":"Department of Integrative Medical Biology, Ume\u00e5 University, SE-901 87 Ume\u00e5, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andre","family":"Lundgren","sequence":"additional","affiliation":[{"name":"Department of Integrative Medical Biology, Ume\u00e5 University, SE-901 87 Ume\u00e5, Sweden"},{"name":"Department of Surgical and Perioperative Science, Section of Hand and Plastic Surgery, Ume\u00e5 University, SE-901 87 Ume\u00e5, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luis Oliveros","family":"Anerillas","sequence":"additional","affiliation":[{"name":"Department of Integrative Medical Biology, Ume\u00e5 University, SE-901 87 Ume\u00e5, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1594-1738","authenticated-orcid":false,"given":"Peyman","family":"Kelk","sequence":"additional","affiliation":[{"name":"Department of Integrative Medical Biology, Ume\u00e5 University, SE-901 87 Ume\u00e5, Sweden"},{"name":"Department of Odontology, Ume\u00e5 University, SE-901 87 Ume\u00e5, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria","family":"Brohlin","sequence":"additional","affiliation":[{"name":"Department of Clinical Microbiology, Ume\u00e5 University, SE-901 87 Ume\u00e5, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mikael","family":"Wiberg","sequence":"additional","affiliation":[{"name":"Department of Integrative Medical Biology, Ume\u00e5 University, SE-901 87 Ume\u00e5, Sweden"},{"name":"Department of Surgical and Perioperative Science, Section of Hand and Plastic Surgery, Ume\u00e5 University, SE-901 87 Ume\u00e5, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2596-5936","authenticated-orcid":false,"given":"Paul J.","family":"Kingham","sequence":"additional","affiliation":[{"name":"Department of Integrative Medical Biology, Ume\u00e5 University, SE-901 87 Ume\u00e5, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ludmila N.","family":"Novikova","sequence":"additional","affiliation":[{"name":"Department of Integrative Medical Biology, Ume\u00e5 University, SE-901 87 Ume\u00e5, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gustav","family":"Andersson","sequence":"additional","affiliation":[{"name":"Department of Integrative Medical Biology, Ume\u00e5 University, SE-901 87 Ume\u00e5, Sweden"},{"name":"Department of Surgical and Perioperative Science, Section of Hand and Plastic Surgery, Ume\u00e5 University, SE-901 87 Ume\u00e5, Sweden"},{"name":"Wallenberg Centre for Molecular Medicine, Ume\u00e5 University, SE-901 87 Ume\u00e5, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5520-3890","authenticated-orcid":false,"given":"Lev N.","family":"Novikov","sequence":"additional","affiliation":[{"name":"Department of Integrative Medical Biology, Ume\u00e5 University, SE-901 87 Ume\u00e5, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,8,6]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"106","DOI":"10.1016\/j.expneurol.2017.01.009","article-title":"Surgical repair in humans after traumatic nerve injury provides limited functional neural regeneration in adults","volume":"290","author":"Palispis","year":"2017","journal-title":"Exp. 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