{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T05:34:58Z","timestamp":1762061698522,"version":"build-2065373602"},"reference-count":40,"publisher":"MDPI AG","issue":"16","license":[{"start":{"date-parts":[[2022,8,10]],"date-time":"2022-08-10T00:00:00Z","timestamp":1660089600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Institute of Public Health\u2014National Institute of Hygiene","award":["4\/EM\/2017"],"award-info":[{"award-number":["4\/EM\/2017"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Fluoroquinolones (FQs) are recommended as prophylaxis for men undergoing transrectal prostate biopsy (TRUS-Bx). Recent studies suggest a significant share of FQ-resistant rectal flora in post-TRUST-Bx infections. Methods: 435 Enterobacterales isolates from 621 patients attending 12 urological departments in Poland were screened by PCR for PMQR genes. PMQR-positive isolates were tested for quinolone susceptibility and investigated by whole genome sequencing (WGS) methods. Results: In total, 32 (7.35%) E. coli strains with ciprofloxacin MIC in the range 0.125\u201332 mg\/L harbored at least one PMQR gene. qnrS and qnrB were the most frequent genes detected in 16 and 12 isolates, respectively. WGS was performed for 28 of 32 PMQR-producing strains. A variety of serotypes and sequence types (STs) of E. coli was noticed. All strains carried at least one virulence gene. AMR genes that encoded resistance against different classes of antibiotics were identified. Additionally, five of 13 ciprofloxacin-susceptible E. coli had alterations in codon 83 of the GyrA subunits. Conclusion: This study provides information on the common presence of PMQRs among E. coli, which may explain the cause for development of post-TRUS-Bx infections. High numbers of virulence and antimicrobial resistance genes detected show a potential for analysed strains to develop infections.<\/jats:p>","DOI":"10.3390\/ijms23168907","type":"journal-article","created":{"date-parts":[[2022,8,10]],"date-time":"2022-08-10T09:42:56Z","timestamp":1660124576000},"page":"8907","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Prevalence of Plasmid-Mediated Quinolone Resistance (PMQRs) Determinants and Whole Genome Sequence Screening of PMQR-Producing E. coli Isolated from Men Undergoing a Transrectal Prostate Biopsy"],"prefix":"10.3390","volume":"23","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1763-3475","authenticated-orcid":false,"given":"Katarzyna","family":"Piekarska","sequence":"first","affiliation":[{"name":"Department of Bacteriology and Biocontamination Control, National Institute of Public Health NIH\u2014National Research Institute, 24 Chocimska Str., 00-791 Warsaw, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1476-2172","authenticated-orcid":false,"given":"Katarzyna","family":"Zacharczuk","sequence":"additional","affiliation":[{"name":"Department of Bacteriology and Biocontamination Control, National Institute of Public Health NIH\u2014National Research Institute, 24 Chocimska Str., 00-791 Warsaw, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8897-4210","authenticated-orcid":false,"given":"Tomasz","family":"Wo\u0142kowicz","sequence":"additional","affiliation":[{"name":"Department of Bacteriology and Biocontamination Control, National Institute of Public Health NIH\u2014National Research Institute, 24 Chocimska Str., 00-791 Warsaw, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2106-1203","authenticated-orcid":false,"given":"Rafa\u0142","family":"Gierczy\u0144ski","sequence":"additional","affiliation":[{"name":"Department of Bacteriology and Biocontamination Control, National Institute of Public Health NIH\u2014National Research Institute, 24 Chocimska Str., 00-791 Warsaw, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,8,10]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1093\/cid\/cit193","article-title":"Infectious complications following transrectal ultrasound-guided prostate biopsy: New challenges in the era of multidrug-resistant Escherichia Coli","volume":"57","author":"Williamson","year":"2013","journal-title":"Clin. 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