{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,8]],"date-time":"2026-07-08T15:11:58Z","timestamp":1783523518817,"version":"3.55.0"},"reference-count":77,"publisher":"MDPI AG","issue":"16","license":[{"start":{"date-parts":[[2022,8,10]],"date-time":"2022-08-10T00:00:00Z","timestamp":1660089600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001665","name":"Agence Nationale de la Recherche of France","doi-asserted-by":"publisher","award":["ANR-16-CE12-0029"],"award-info":[{"award-number":["ANR-16-CE12-0029"]}],"id":[{"id":"10.13039\/501100001665","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001665","name":"Agence Nationale de la Recherche of France","doi-asserted-by":"publisher","award":["BIO2016-75235-P"],"award-info":[{"award-number":["BIO2016-75235-P"]}],"id":[{"id":"10.13039\/501100001665","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001665","name":"Agence Nationale de la Recherche of France","doi-asserted-by":"publisher","award":["PID2020-116995RB-I00"],"award-info":[{"award-number":["PID2020-116995RB-I00"]}],"id":[{"id":"10.13039\/501100001665","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Ministerio de Ciencia e Innovaci\u00f3n of Spain (Agencia Estatal de Investigaci\u00f3n)","award":["ANR-16-CE12-0029"],"award-info":[{"award-number":["ANR-16-CE12-0029"]}]},{"name":"Ministerio de Ciencia e Innovaci\u00f3n of Spain (Agencia Estatal de Investigaci\u00f3n)","award":["BIO2016-75235-P"],"award-info":[{"award-number":["BIO2016-75235-P"]}]},{"name":"Ministerio de Ciencia e Innovaci\u00f3n of Spain (Agencia Estatal de Investigaci\u00f3n)","award":["PID2020-116995RB-I00"],"award-info":[{"award-number":["PID2020-116995RB-I00"]}]},{"name":"European Regional Fund","award":["ANR-16-CE12-0029"],"award-info":[{"award-number":["ANR-16-CE12-0029"]}]},{"name":"European Regional Fund","award":["BIO2016-75235-P"],"award-info":[{"award-number":["BIO2016-75235-P"]}]},{"name":"European Regional Fund","award":["PID2020-116995RB-I00"],"award-info":[{"award-number":["PID2020-116995RB-I00"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Salmonella enterica, a Gram-negative zoonotic bacterium, is mainly a food-borne pathogen and the main cause of diarrhea in humans worldwide. The main reservoirs are found in poultry farms, but they are also found in wild birds. The development of antibiotic resistance in S. enterica species raises concerns about the future of efficient therapies against this pathogen and revives the interest in bacteriophages as a useful therapy against bacterial infections. Here, we aimed to decipher and functionally annotate 10 new Salmonella phage genomes isolated in Spain in the light of phage therapy. We designed a bioinformatic pipeline using available building blocks to de novo assemble genomes and perform syntaxic annotation. We then used genome-wide analyses for taxonomic annotation enabled by vContact2 and VICTOR. We were also particularly interested in improving functional annotation using remote homologies detection and comparisons with the recently published phage-specific PHROG protein database. Finally, we searched for useful functions for phage therapy, such as systems encoded by the phage to circumvent cellular defenses with a particular focus on anti-CRISPR proteins. We, thus, were able to genetically characterize nine virulent phages and one temperate phage and identify putative functions relevant to the formulation of phage cocktails for Salmonella biocontrol.<\/jats:p>","DOI":"10.3390\/ijms23168917","type":"journal-article","created":{"date-parts":[[2022,8,10]],"date-time":"2022-08-10T09:42:56Z","timestamp":1660124576000},"page":"8917","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Genetic Mining of Newly Isolated Salmophages for Phage Therapy"],"prefix":"10.3390","volume":"23","author":[{"given":"Julia","family":"Gendre","sequence":"first","affiliation":[{"name":"Laboratoire de Chimie Bact\u00e9rienne, (UMR7283)-CNRS\/Aix-Marseille Universit\u00e9, 13009 Marseille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2878-0702","authenticated-orcid":false,"given":"Mireille","family":"Ansaldi","sequence":"additional","affiliation":[{"name":"Laboratoire de Chimie Bact\u00e9rienne, (UMR7283)-CNRS\/Aix-Marseille Universit\u00e9, 13009 Marseille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David R.","family":"Olivenza","sequence":"additional","affiliation":[{"name":"Departamento de Gen\u00e9tica, Facultad de Biologia, Universidad de Sevilla, 41012 Sevilla, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yann","family":"Denis","sequence":"additional","affiliation":[{"name":"Institut de Microbiologie de la M\u00e9diterran\u00e9e, (FR3479)-CNRS\/Aix-Marseille Universit\u00e9, 13009 Marseille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Josep","family":"Casades\u00fas","sequence":"additional","affiliation":[{"name":"Departamento de Gen\u00e9tica, Facultad de Biologia, Universidad de Sevilla, 41012 Sevilla, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5544-4376","authenticated-orcid":false,"given":"Nicolas","family":"Ginet","sequence":"additional","affiliation":[{"name":"Laboratoire de Chimie Bact\u00e9rienne, (UMR7283)-CNRS\/Aix-Marseille Universit\u00e9, 13009 Marseille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,8,10]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"629","DOI":"10.1016\/S0140-6736(21)02724-0","article-title":"Global Burden of Bacterial Antimicrobial Resistance in 2019: A Systematic Analysis","volume":"399","author":"Murray","year":"2022","journal-title":"Lancet"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1241","DOI":"10.1016\/S0140-6736(01)20383-3","article-title":"An Investigation on the Nature of Ultra-Microscopic Viruses","volume":"186","author":"Twort","year":"1915","journal-title":"Lancet"},{"key":"ref_3","first-page":"373","article-title":"Sur un Microbe Invisible Antagoniste des Bacilles Dysent\u00e9riques","volume":"165","year":"1917","journal-title":"C. 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