{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,5]],"date-time":"2026-06-05T14:54:14Z","timestamp":1780671254563,"version":"3.54.1"},"reference-count":26,"publisher":"MDPI AG","issue":"16","license":[{"start":{"date-parts":[[2022,8,11]],"date-time":"2022-08-11T00:00:00Z","timestamp":1660176000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"ADIR","award":["N\u00b0ADIR-2016-07"],"award-info":[{"award-number":["N\u00b0ADIR-2016-07"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>The routine use of mechanical circulatory support during lung transplantation (LTx) is still controversial. The use of prophylactic human albumin (HA) or hypertonic sodium lactate (HSL) prime in mechanical circulatory support during LTx could prevent ischemia\u2013reperfusion (IR) injuries and pulmonary endothelial dysfunction and thus prevent the development of pulmonary graft dysfunction. The objective was to investigate the impact of cardiopulmonary bypass (CPB) priming with HA and HSL compared to a CPB prime with Gelofusine (GF) on pulmonary endothelial dysfunction in a lung IR rat model. Rats were assigned to four groups: IR-CPB-GF group, IR-CPB-HA group, IR-CPB-HSL group and a sham group. The study of pulmonary vascular reactivity by wire myograph was the primary outcome. Glycocalyx degradation (syndecan-1 and heparan) was also assessed by ELISA and electron microscopy, systemic and pulmonary inflammation by ELISA (IL-1\u03b2, IL-10, and TNF-\u03b1) and immunohistochemistry. Clinical parameters were evaluated. We employed a CPB model with three different primings, permitting femoral\u2013femoral assistance with left pulmonary hilum ischemia for IR. Pulmonary endothelium-dependent relaxation to acetylcholine was significantly decreased in the IR-CPB-GF group (11.9 \u00b1 6.2%) compared to the IR-CPB-HA group (52.8 \u00b1 5.2%, p &lt; 0.0001), the IR-CPB-HSL group (57.7 \u00b1 6.3%, p &lt; 0.0001) and the sham group (80.8 \u00b1 6.5%, p &lt; 0.0001). We did not observe any difference between the groups concerning glycocalyx degradation, and systemic or tissular inflammation. The IR-CPB-HSL group needed more vascular filling and developed significantly more pulmonary edema than the IR-CPB-GF group and the IR-CPB-HA group. Using HA as a prime in CPB during Ltx could decrease pulmonary endothelial dysfunction\u2019s IR-mediated effects. No effects of HA were found on inflammation.<\/jats:p>","DOI":"10.3390\/ijms23168938","type":"journal-article","created":{"date-parts":[[2022,8,11]],"date-time":"2022-08-11T05:43:57Z","timestamp":1660196637000},"page":"8938","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Priming of Cardiopulmonary Bypass with Human Albumin Decreases Endothelial Dysfunction after Pulmonary Ischemia\u2013Reperfusion in an Animal Model"],"prefix":"10.3390","volume":"23","author":[{"given":"Jean","family":"Selim","sequence":"first","affiliation":[{"name":"Normandie University, UNIROUEN, INSERM U1096, 76000 Rouen, France"},{"name":"Department of Anesthesiology and Critical Care, Rouen University Hospital, 76000 Rouen, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7681-4866","authenticated-orcid":false,"given":"Mouad","family":"Hamzaoui","sequence":"additional","affiliation":[{"name":"Normandie University, UNIROUEN, INSERM U1096, 76000 Rouen, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antoine","family":"Ghemired","sequence":"additional","affiliation":[{"name":"Normandie University, UNIROUEN, INSERM U1096, 76000 Rouen, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4022-7889","authenticated-orcid":false,"given":"Zoubir","family":"Djerada","sequence":"additional","affiliation":[{"name":"Normandie University, UNIROUEN, INSERM U1096, 76000 Rouen, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laurence","family":"Chevalier","sequence":"additional","affiliation":[{"name":"Normandie University, UNIROUEN, CNRS, GPM-UMR 6634, 76000 Rouen, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8779-586X","authenticated-orcid":false,"given":"Nicolas","family":"Piton","sequence":"additional","affiliation":[{"name":"Department of Pathology, Rouen University Hospital, 76000 Rouen, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emmanuel","family":"Besnier","sequence":"additional","affiliation":[{"name":"Normandie University, UNIROUEN, INSERM U1096, 76000 Rouen, France"},{"name":"Department of Anesthesiology and Critical Care, Rouen University Hospital, 76000 Rouen, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"Clavier","sequence":"additional","affiliation":[{"name":"Normandie University, UNIROUEN, INSERM U1096, 76000 Rouen, France"},{"name":"Department of Anesthesiology and Critical Care, Rouen University Hospital, 76000 Rouen, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana\u00efs","family":"Dumesnil","sequence":"additional","affiliation":[{"name":"Normandie University, UNIROUEN, INSERM U1096, 76000 Rouen, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sylvanie","family":"Renet","sequence":"additional","affiliation":[{"name":"Normandie University, UNIROUEN, INSERM U1096, 76000 Rouen, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul","family":"Mulder","sequence":"additional","affiliation":[{"name":"Normandie University, UNIROUEN, INSERM U1096, 76000 Rouen, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0671-6870","authenticated-orcid":false,"given":"Fabien","family":"Doguet","sequence":"additional","affiliation":[{"name":"Normandie University, UNIROUEN, INSERM U1096, 76000 Rouen, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3708-1460","authenticated-orcid":false,"given":"Fabienne","family":"Tamion","sequence":"additional","affiliation":[{"name":"Normandie University, UNIROUEN, INSERM U1096, 76000 Rouen, France"},{"name":"Department of Medical Intensive Care, Rouen University Hospital, 76000 Rouen, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Beno\u00eet","family":"Veber","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology and Critical Care, Rouen University Hospital, 76000 Rouen, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0383-2342","authenticated-orcid":false,"given":"J\u00e9r\u00e9my","family":"Bellien","sequence":"additional","affiliation":[{"name":"Normandie University, UNIROUEN, INSERM U1096, 76000 Rouen, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vincent","family":"Richard","sequence":"additional","affiliation":[{"name":"Normandie University, UNIROUEN, INSERM U1096, 76000 Rouen, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Marc","family":"Baste","sequence":"additional","affiliation":[{"name":"Normandie University, UNIROUEN, INSERM U1096, 76000 Rouen, France"},{"name":"Department of Thoracic Surgery, Rouen University Hospital, 76000 Rouen, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,8,11]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1055\/s-0030-1249111","article-title":"Primary Graft Dysfunction: Definition, Risk Factors, Short- and Long-Term Outcomes","volume":"31","author":"Lee","year":"2010","journal-title":"Semin. 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Allergy Drug Discov."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1753","DOI":"10.2353\/ajpath.2007.070316","article-title":"Human Serum Albumin Improves Arterial Dysfunction during Early Resuscitation in Mouse Endotoxic Model via Reduced Oxidative and Nitrosative Stresses","volume":"171","author":"Meziani","year":"2007","journal-title":"Am. J. Pathol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1414","DOI":"10.1097\/CCM.0b013e318211ff6e","article-title":"Human Serum Albumin Improves Endothelial Dysfunction and Survival during Experimental Endotoxemia: Concentration-Dependent Properties","volume":"39","author":"Kremer","year":"2011","journal-title":"Crit. Care Med."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"252","DOI":"10.1177\/1089253214535667","article-title":"Albumin-Beyond Fluid Replacement in Cardiopulmonary Bypass Surgery: Why, How, and When?","volume":"18","author":"Moret","year":"2014","journal-title":"Semin. Cardiothorac Vasc. 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Surg."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1016\/j.morpho.2017.04.001","article-title":"Electron Microscopy Approach for the Visualization of the Epithelial and Endothelial Glycocalyx","volume":"101","author":"Chevalier","year":"2017","journal-title":"Morphologie"}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/23\/16\/8938\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T00:07:06Z","timestamp":1760141226000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/23\/16\/8938"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,8,11]]},"references-count":26,"journal-issue":{"issue":"16","published-online":{"date-parts":[[2022,8]]}},"alternative-id":["ijms23168938"],"URL":"https:\/\/doi.org\/10.3390\/ijms23168938","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,8,11]]}}}