{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T08:43:00Z","timestamp":1790066580297,"version":"4.0.1"},"reference-count":61,"publisher":"MDPI AG","issue":"16","license":[{"start":{"date-parts":[[2022,8,11]],"date-time":"2022-08-11T00:00:00Z","timestamp":1660176000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"French National Research Agency (Agence Nationale de Recherche\u2014ANR)","award":["2014-A00298-39: 2014\u20132017"],"award-info":[{"award-number":["2014-A00298-39: 2014\u20132017"]}]},{"name":"French National Research Agency (Agence Nationale de Recherche\u2014ANR)","award":["ANR-15-RHU-0004"],"award-info":[{"award-number":["ANR-15-RHU-0004"]}]},{"name":"Future Program","award":["2014-A00298-39: 2014\u20132017"],"award-info":[{"award-number":["2014-A00298-39: 2014\u20132017"]}]},{"name":"Future Program","award":["ANR-15-RHU-0004"],"award-info":[{"award-number":["ANR-15-RHU-0004"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Endothelial dysfunction is a key factor in atherosclerosis. However, the link between endothelial repair and severity of atherosclerotic cardiovascular disease (ASCVD) is unclear. This study investigates the relationship between ASCVD, markers of inflammation, and circulating endothelial progenitor cells, namely hematopoietic cells with paracrine angiogenic activity and endothelial colony forming cells (ECFC). Two hundred and forty-three subjects from the TELARTA study were classified according to the presence of clinical atherosclerotic disease. ASCVD severity was assessed by the number of involved vascular territories. Flow cytometry was used to numerate circulating progenitor cells (PC) expressing CD34 and those co-expressing CD45, CD34, and KDR. Peripheral blood mononuclear cells ex vivo culture methods were used to determine ECFC and Colony Forming Unit- endothelial cells (CFU-EC). The ECFC subpopulation was analyzed for proliferation, senescence, and vasculogenic properties. Plasma levels of IL-6 and VEGF-A were measured using Cytokine Array. Despite an increased number of circulating precursors in ASCVD patients, ASCVD impaired the colony forming capacity and the angiogenic properties of ECFC in a severity-dependent manner. Alteration of ECFC was associated with increased senescent phenotype and IL-6 levels. Our study demonstrates a decrease in ECFC repair capacity according to ASCVD severity in an inflammatory and senescence-associated secretory phenotype context.<\/jats:p>","DOI":"10.3390\/ijms23168969","type":"journal-article","created":{"date-parts":[[2022,8,11]],"date-time":"2022-08-11T21:15:05Z","timestamp":1660252505000},"page":"8969","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Functional Impairment of Endothelial Colony Forming Cells (ECFC) in Patients with Severe Atherosclerotic Cardiovascular Disease (ASCVD)"],"prefix":"10.3390","volume":"23","author":[{"given":"St\u00e9phanie","family":"Simoncini","sequence":"first","affiliation":[{"name":"Aix Marseille Univ, INSERM, INRAE, C2VN, 13005 Marseille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7441-567X","authenticated-orcid":false,"given":"Simon","family":"Toupance","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Lorraine, INSERM, DCAC, 54000 Nancy, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5275-3405","authenticated-orcid":false,"given":"Carlos","family":"Labat","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Lorraine, INSERM, DCAC, 54000 Nancy, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sylvie","family":"Gautier","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Lorraine, CHRU-Nancy, Department of Geriatrics, 54000 Nancy, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chlo\u00e9","family":"Dumoulin","sequence":"additional","affiliation":[{"name":"Cell Therapy Laboratory, H\u00f4pital de la Conception, AP-HM, INSERM CIC BT 1409, 13005 Marseille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laurent","family":"Arnaud","sequence":"additional","affiliation":[{"name":"Hematology and Vascular Biology Department, Hopital de la Timone, AP-HM, 13005 Marseille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria G.","family":"Stathopoulou","sequence":"additional","affiliation":[{"name":"INSERM, C3M, Team Control of Gene Expression (10), Universit\u00e9 Cote d\u2019Azur, 06000 Nice, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8104-8425","authenticated-orcid":false,"given":"Sophie","family":"Visvikis-Siest","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Lorraine, IGE-PCV, 54000 Nancy, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pascal M.","family":"Rossi","sequence":"additional","affiliation":[{"name":"Aix Marseille Univ, INSERM, INRAE, C2VN, 13005 Marseille, France"},{"name":"Service de M\u00e9decine Interne, H\u00f4pital Nord, APHM, 13015 Marseille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Athanase","family":"Benetos","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Lorraine, INSERM, DCAC, 54000 Nancy, France"},{"name":"Universit\u00e9 de Lorraine, CHRU-Nancy, Department of Geriatrics, 54000 Nancy, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fran\u00e7oise","family":"Dignat-George","sequence":"additional","affiliation":[{"name":"Aix Marseille Univ, INSERM, INRAE, C2VN, 13005 Marseille, France"},{"name":"Hematology and Vascular Biology Department, Hopital de la Timone, AP-HM, 13005 Marseille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Florence","family":"Sabatier","sequence":"additional","affiliation":[{"name":"Aix Marseille Univ, INSERM, INRAE, C2VN, 13005 Marseille, France"},{"name":"Cell Therapy Laboratory, H\u00f4pital de la Conception, AP-HM, INSERM CIC BT 1409, 13005 Marseille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,8,11]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1016\/j.aogh.2013.12.001","article-title":"Regression of Atherosclerosis: Insights from Animal and Clinical Studies","volume":"80","author":"Feig","year":"2014","journal-title":"Ann. 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