{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,23]],"date-time":"2026-07-23T17:35:09Z","timestamp":1784828109902,"version":"3.55.0"},"reference-count":168,"publisher":"MDPI AG","issue":"16","license":[{"start":{"date-parts":[[2022,8,14]],"date-time":"2022-08-14T00:00:00Z","timestamp":1660435200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004410","name":"Scientific and Technical Research Council of Turkey (TUBITAK)","doi-asserted-by":"publisher","award":["119Z208"],"award-info":[{"award-number":["119Z208"]}],"id":[{"id":"10.13039\/501100004410","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>New insights into the interactions between nanopesticides and edible plants are required in order to elucidate their impacts on human health and agriculture. Nanopesticides include formulations consisting of organic\/inorganic nanoparticles. Drosophila melanogaster has become a powerful model in genetic research thanks to its genetic similarity to mammals. This project mainly aimed to generate new evidence for the toxic\/genotoxic properties of different nanopesticides (a nanoemulsion (permethrin nanopesticides, 20 \u00b1 5 nm), an inorganic nanoparticle as an active ingredient (copper(II) hydroxide [Cu(OH)2] nanopesticides, 15 \u00b1 6 nm), a polymer-based nanopesticide (acephate nanopesticides, 55 \u00b1 25 nm), and an inorganic nanoparticle associated with an organic active ingredient (validamycin nanopesticides, 1177 \u00b1 220 nm)) and their microparticulate forms (i.e., permethrin, copper(II) sulfate pentahydrate (CuSO4\u00b75H2O), acephate, and validamycin) widely used against agricultural pests, while also showing the merits of using Drosophila\u2014a non-target in vivo eukaryotic model organism\u2014in nanogenotoxicology studies. Significant biological effects were noted at the highest doses of permethrin (0.06 and 0.1 mM), permethrin nanopesticides (1 and 2.5 mM), CuSO4\u00b75H2O (1 and 5 mM), acephate and acephate nanopesticides (1 and 5 mM, respectively), and validamycin and validamycin nanopesticides (1 and 2.5 mM, respectively). The results demonstrating the toxic\/genotoxic potential of these nanopesticides through their impact on cellular internalization and gene expression represent significant contributions to future nanogenotoxicology studies.<\/jats:p>","DOI":"10.3390\/ijms23169121","type":"journal-article","created":{"date-parts":[[2022,8,15]],"date-time":"2022-08-15T01:47:21Z","timestamp":1660528041000},"page":"9121","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Hazard Assessment of the Effects of Acute and Chronic Exposure to Permethrin, Copper Hydroxide, Acephate, and Validamycin Nanopesticides on the Physiology of Drosophila: Novel Insights into the Cellular Internalization and Biological Effects"],"prefix":"10.3390","volume":"23","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2146-7385","authenticated-orcid":false,"given":"E\u015fref","family":"Demir","sequence":"first","affiliation":[{"name":"Medical Laboratory Techniques Program, Vocational School of Health Services, Department of Medical Services and Techniques, Antalya Bilim University, Antalya 07190, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seyithan","family":"Kans\u0131z","sequence":"additional","affiliation":[{"name":"Faculty of Science, Department of Chemistry, Akdeniz University, Antalya 07070, Turkey"},{"name":"Faculty of Science, Department of Chemistry, Ankara University, Ankara 07100, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8497-1829","authenticated-orcid":false,"given":"Mehmet","family":"Do\u011fan","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, Department of Histology and Embryology, Akdeniz University, Antalya 07070, Turkey"},{"name":"Department of Histology and Embryology, Faculty of Medicine, K\u0131rklareli University, K\u0131rklareli 39100, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1584-5519","authenticated-orcid":false,"given":"\u00d6nder","family":"Topel","sequence":"additional","affiliation":[{"name":"Faculty of Science, Department of Chemistry, Akdeniz University, Antalya 07070, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G\u00f6khan","family":"Akkoyunlu","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, Department of Histology and Embryology, Akdeniz University, Antalya 07070, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muhammed Yusuf","family":"Kandur","sequence":"additional","affiliation":[{"name":"Industrial Biotechnology and Systems Biology Research Group, Faculty of Engineering, Department of Bioengineering, Marmara University, \u0130stanbul 34854, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fatma","family":"Turna Demir","sequence":"additional","affiliation":[{"name":"Medical Laboratory Techniques Program, Vocational School of Health Services, Department of Medical Services and Techniques, Antalya Bilim University, Antalya 07190, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,8,14]]},"reference":[{"key":"ref_1","unstructured":"United Nations (2017). 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