{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T00:01:21Z","timestamp":1790726481584,"version":"4.1.0"},"reference-count":46,"publisher":"MDPI AG","issue":"16","license":[{"start":{"date-parts":[[2022,8,17]],"date-time":"2022-08-17T00:00:00Z","timestamp":1660694400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001674","name":"Leducq Transatlantic Network of Excellence \u201cTargeting Mitochondria to Treat Heart Disease \u2018MitoCardia\u201d","doi-asserted-by":"publisher","award":["16 CVD 04"],"award-info":[{"award-number":["16 CVD 04"]}],"id":[{"id":"10.13039\/501100001674","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Hospices Civils de Lyon \u201cAnn\u00e9e M\u00e9daille d\u2019Or 2021\u201d","award":["16 CVD 04"],"award-info":[{"award-number":["16 CVD 04"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Renal ischemia-reperfusion (IR) injury can lead to acute kidney injury, increasing the risk of developing chronic kidney disease. We hypothesized that mild therapeutic hypothermia (mTH), 34 \u00b0C, applied during ischemia could protect the function and structure of kidneys against IR injuries in mice. In vivo bilateral renal IR led to an increase in plasma urea and acute tubular necrosis at 24 h prevented by mTH. One month after unilateral IR, kidney atrophy and fibrosis were reduced by mTH. Evaluation of mitochondrial function showed that mTH protected against IR-mediated mitochondrial dysfunction at 24 h, by preserving CRC and OX-PHOS. mTH completely abrogated the IR increase of plasmatic IL-6 and IL-10 at 24 h. Acute tissue inflammation was decreased by mTH (IL-6 and IL1-\u03b2) in as little as 2 h. Concomitantly, mTH increased TNF-\u03b1 expression at 24 h. One month after IR, mTH increased TNF-\u03b1 mRNA expression, and it decreased TGF-\u03b2 mRNA expression. We showed that mTH alleviates renal dysfunction and damage through a preservation of mitochondrial function and a modulated systemic and local inflammatory response at the acute phase (2\u201324 h). The protective effect of mTH is maintained in the long term (1 month), as it diminished renal atrophy and fibrosis, and mitigated chronic renal inflammation.<\/jats:p>","DOI":"10.3390\/ijms23169229","type":"journal-article","created":{"date-parts":[[2022,8,17]],"date-time":"2022-08-17T21:23:56Z","timestamp":1660771436000},"page":"9229","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["Mild Therapeutic Hypothermia Protects from Acute and Chronic Renal Ischemia-Reperfusion Injury in Mice by Mitigated Mitochondrial Dysfunction and Modulation of Local and Systemic Inflammation"],"prefix":"10.3390","volume":"23","author":[{"given":"Maxime","family":"Schleef","sequence":"first","affiliation":[{"name":"CarMeN Laboratory, Univ Lyon, INSERM, INRA, INSA Lyon, Universit\u00e9 Claude Bernard Lyon 1, 69500 Bron, France"},{"name":"Hospices Civils de Lyon, M\u00e9decine Intensive R\u00e9animation, H\u00f4pital Edouard Herriot, 69003 Lyon, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fabrice","family":"Gonnot","sequence":"additional","affiliation":[{"name":"CarMeN Laboratory, Univ Lyon, INSERM, INRA, INSA Lyon, Universit\u00e9 Claude Bernard Lyon 1, 69500 Bron, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bruno","family":"Pillot","sequence":"additional","affiliation":[{"name":"CarMeN Laboratory, Univ Lyon, INSERM, INRA, INSA Lyon, Universit\u00e9 Claude Bernard Lyon 1, 69500 Bron, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christelle","family":"Leon","sequence":"additional","affiliation":[{"name":"CarMeN Laboratory, Univ Lyon, INSERM, INRA, INSA Lyon, Universit\u00e9 Claude Bernard Lyon 1, 69500 Bron, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6930-6671","authenticated-orcid":false,"given":"St\u00e9phanie","family":"Chanon","sequence":"additional","affiliation":[{"name":"CarMeN Laboratory, Univ Lyon, INSERM, INRA, INSA Lyon, Universit\u00e9 Claude Bernard Lyon 1, 69500 Bron, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4382-8735","authenticated-orcid":false,"given":"Aur\u00e9lie","family":"Vieille-Marchiset","sequence":"additional","affiliation":[{"name":"CarMeN Laboratory, Univ Lyon, INSERM, INRA, INSA Lyon, Universit\u00e9 Claude Bernard Lyon 1, 69500 Bron, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0713-3271","authenticated-orcid":false,"given":"Maud","family":"Rabeyrin","sequence":"additional","affiliation":[{"name":"Hospices Civils de Lyon, Anatomopathologie, Groupement Hospitalier Est, 69500 Bron, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabriel","family":"Bidaux","sequence":"additional","affiliation":[{"name":"CarMeN Laboratory, Univ Lyon, INSERM, INRA, INSA Lyon, Universit\u00e9 Claude Bernard Lyon 1, 69500 Bron, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fitsum","family":"Guebre-Egziabher","sequence":"additional","affiliation":[{"name":"CarMeN Laboratory, Univ Lyon, INSERM, INRA, INSA Lyon, Universit\u00e9 Claude Bernard Lyon 1, 69500 Bron, France"},{"name":"Hospices Civils de Lyon, N\u00e9phrologie-HTA-Dialyse, H\u00f4pital Edouard Herriot, 69003 Lyon, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laurent","family":"Juillard","sequence":"additional","affiliation":[{"name":"CarMeN Laboratory, Univ Lyon, INSERM, INRA, INSA Lyon, Universit\u00e9 Claude Bernard Lyon 1, 69500 Bron, France"},{"name":"Hospices Civils de Lyon, N\u00e9phrologie-HTA-Dialyse, H\u00f4pital Edouard Herriot, 69003 Lyon, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7678-062X","authenticated-orcid":false,"given":"Delphine","family":"Baetz","sequence":"additional","affiliation":[{"name":"CarMeN Laboratory, Univ Lyon, INSERM, INRA, INSA Lyon, Universit\u00e9 Claude Bernard Lyon 1, 69500 Bron, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sandrine","family":"Lemoine","sequence":"additional","affiliation":[{"name":"CarMeN Laboratory, Univ Lyon, INSERM, INRA, INSA Lyon, Universit\u00e9 Claude Bernard Lyon 1, 69500 Bron, France"},{"name":"Hospices Civils de Lyon, Explorations Fonctionnelles R\u00e9nales, H\u00f4pital Edouard Herriot, 69003 Lyon, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,8,17]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1391","DOI":"10.1038\/nm.2507","article-title":"Ischemia and reperfusion\u2014From mechanism to translation","volume":"17","author":"Eltzschig","year":"2011","journal-title":"Nat. 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