{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T18:58:13Z","timestamp":1790449093364,"version":"4.1.0"},"reference-count":54,"publisher":"MDPI AG","issue":"16","license":[{"start":{"date-parts":[[2022,8,18]],"date-time":"2022-08-18T00:00:00Z","timestamp":1660780800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"MITECO and Fundaci\u00f3n Biodiversidad","award":["PCRV00820"],"award-info":[{"award-number":["PCRV00820"]}]},{"name":"MITECO and Fundaci\u00f3n Biodiversidad","award":["INFRAIA-731014"],"award-info":[{"award-number":["INFRAIA-731014"]}]},{"name":"MITECO and Fundaci\u00f3n Biodiversidad","award":["PID2019-107145RB-I00"],"award-info":[{"award-number":["PID2019-107145RB-I00"]}]},{"name":"European Union\u2019s Horizon 2020 Research and Innovation program VetBioNet","award":["PCRV00820"],"award-info":[{"award-number":["PCRV00820"]}]},{"name":"European Union\u2019s Horizon 2020 Research and Innovation program VetBioNet","award":["INFRAIA-731014"],"award-info":[{"award-number":["INFRAIA-731014"]}]},{"name":"European Union\u2019s Horizon 2020 Research and Innovation program VetBioNet","award":["PID2019-107145RB-I00"],"award-info":[{"award-number":["PID2019-107145RB-I00"]}]},{"name":"MCIN\/AEI\/10.13039\/501100011033","award":["PCRV00820"],"award-info":[{"award-number":["PCRV00820"]}]},{"name":"MCIN\/AEI\/10.13039\/501100011033","award":["INFRAIA-731014"],"award-info":[{"award-number":["INFRAIA-731014"]}]},{"name":"MCIN\/AEI\/10.13039\/501100011033","award":["PID2019-107145RB-I00"],"award-info":[{"award-number":["PID2019-107145RB-I00"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>The European mink (Mustela lutreola) is one of Europe\u2019s most endangered species, and it is on the brink of extinction in the Iberian Peninsula. The species\u2019 precarious situation requires the application of new ex situ conservation methodologies that complement the existing ex situ and in situ conservation measures. Here, we report for the first time the establishment of a biobank for European mink mesenchymal stem cells (emMSC) and oocytes from specimens found dead in the Iberian Peninsula, either free or in captivity. New emMSC lines were isolated from different tissues: bone marrow (emBM-MSC), oral mucosa (emOM-MSc), dermal skin (emDS-MSC), oviduct (emO-MSc), endometrium (emE-MSC), testicular (emT-MSC), and adipose tissue from two different adipose depots: subcutaneous (emSCA-MSC) and ovarian (emOA-MSC). All eight emMSC lines showed plastic adhesion, a detectable expression of characteristic markers of MSCs, and, when cultured under osteogenic and adipogenic conditions, differentiation capacity to these lineages. Additionally, we were able to keep 227 Cumulus-oocyte complexes (COCs) in the biobank, 97 of which are grade I or II. The European mink MSC and oocyte biobank will allow for the conservation of the species\u2019 genetic variability, the application of assisted reproduction techniques, and the development of in vitro models for studying the molecular mechanisms of infectious diseases that threaten the species\u2019 precarious situation.<\/jats:p>","DOI":"10.3390\/ijms23169319","type":"journal-article","created":{"date-parts":[[2022,8,18]],"date-time":"2022-08-18T21:39:21Z","timestamp":1660858761000},"page":"9319","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Cryobanking European Mink (Mustela lutreola) Mesenchymal Stem Cells and Oocytes"],"prefix":"10.3390","volume":"23","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3903-5019","authenticated-orcid":false,"given":"Alexandra","family":"Calle","sequence":"first","affiliation":[{"name":"Department of Animal Reproduction, National Institute for Agriculture and Food Research and Technology (INIA), CSIC, 28040 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5868-2134","authenticated-orcid":false,"given":"Miguel \u00c1ngel","family":"Ram\u00edrez","sequence":"additional","affiliation":[{"name":"Department of Animal Reproduction, National Institute for Agriculture and Food Research and Technology (INIA), CSIC, 28040 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,8,18]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"8357","DOI":"10.1128\/jvi.71.11.8357-8361.1997","article-title":"Arginine-glycine-aspartic acid-specific binding by foot-and-mouth disease viruses to the purified integrin alpha(v)beta3 in vitro","volume":"71","author":"Jackson","year":"1997","journal-title":"J. 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