{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T04:48:01Z","timestamp":1787028481092,"version":"build-2736575974"},"reference-count":32,"publisher":"MDPI AG","issue":"16","license":[{"start":{"date-parts":[[2022,8,19]],"date-time":"2022-08-19T00:00:00Z","timestamp":1660867200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004281","name":"Narodowe Centrum Nauki (National Science Center), Poland","doi-asserted-by":"publisher","award":["2018\/31\/B\/NZ6\/02744"],"award-info":[{"award-number":["2018\/31\/B\/NZ6\/02744"]}],"id":[{"id":"10.13039\/501100004281","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Background: The detection rate of viral RNA in tick-borne encephalitis (TBE) is low and variable between studies, and its diagnostic\/prognostic potential is not well defined. We attempted to detect RNA of TBE virus (TBEV) in body fluids of TBE patients. Methods: We studied 98 adults and 12 children with TBEV infection, stratified by the disease phase and presentation. EDTA blood and cerebrospinal fluid (CSF) samples were obtained upon hospital admission. RNA was extracted from freshly obtained plasma, concentrated leukocyte-enriched CSF, and whole blood samples, and real time PCR was performed with a Rotor-Gene Q thermocycler. Results: TBEV RNA was detected in (1) plasma of one (of the two studied) adult patients with an abortive infection, (2) plasma of two (of the two studied) adults in the peripheral phase of TBE, and (3) plasma and blood of an adult in the neurologic phase of TBE presenting as meningoencephalomyelitis. No CSF samples were TBEV RNA-positive. Conclusions: The detection of TBEV RNA in blood might be diagnostic in the peripheral phase of TBE. The lack of TBEV RNA in the CSF cellular fraction speaks against TBEV influx into the central nervous system with infiltrating leukocytes and is consistent with a relatively low intrathecal viral burden.<\/jats:p>","DOI":"10.3390\/ijms23169332","type":"journal-article","created":{"date-parts":[[2022,8,21]],"date-time":"2022-08-21T22:23:13Z","timestamp":1661120593000},"page":"9332","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["The Detectability of the Viral RNA in Blood and Cerebrospinal Fluid of Patients with Tick-Borne Encephalitis"],"prefix":"10.3390","volume":"23","author":[{"given":"Sambor","family":"Grygorczuk","sequence":"first","affiliation":[{"name":"Department of the Infectious Diseases and Neuroinfections, Medical University in Bia\u0142ystok, Ul. \u017burawia 14, 15-540 Bia\u0142ystok, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Justyna","family":"Dunaj-Ma\u0142yszko","sequence":"additional","affiliation":[{"name":"Department of the Infectious Diseases and Neuroinfections, Medical University in Bia\u0142ystok, Ul. \u017burawia 14, 15-540 Bia\u0142ystok, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0072-2180","authenticated-orcid":false,"given":"Piotr","family":"Czupryna","sequence":"additional","affiliation":[{"name":"Department of the Infectious Diseases and Neuroinfections, Medical University in Bia\u0142ystok, Ul. \u017burawia 14, 15-540 Bia\u0142ystok, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8391-4793","authenticated-orcid":false,"given":"Artur","family":"Sulik","sequence":"additional","affiliation":[{"name":"Department of the Pediatric Infectious Diseases of the Medical University in Bia\u0142ystok, Ul. Jerzego Waszyngtona 17, 15-274 Bia\u0142ystok, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kacper","family":"Toczy\u0142owski","sequence":"additional","affiliation":[{"name":"Department of the Pediatric Infectious Diseases of the Medical University in Bia\u0142ystok, Ul. Jerzego Waszyngtona 17, 15-274 Bia\u0142ystok, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Agnieszka","family":"Siemieniako-Werszko","sequence":"additional","affiliation":[{"name":"University Hospital in Bia\u0142ystok, Ul. \u017burawia 14, 15-540 Bia\u0142ystok, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Agnieszka","family":"\u017bebrowska","sequence":"additional","affiliation":[{"name":"Regional Centre of Transfusion Medicine in Bia\u0142ystok, Ul. Marii Sk\u0142odowskiej-Curie 23, 15-950 Bia\u0142ystok, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S\u0142awomir","family":"Pancewicz","sequence":"additional","affiliation":[{"name":"Department of the Infectious Diseases and Neuroinfections, Medical University in Bia\u0142ystok, Ul. \u017burawia 14, 15-540 Bia\u0142ystok, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"Moniuszko-Malinowska","sequence":"additional","affiliation":[{"name":"Department of the Infectious Diseases and Neuroinfections, Medical University in Bia\u0142ystok, Ul. \u017burawia 14, 15-540 Bia\u0142ystok, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,8,19]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"430","DOI":"10.12998\/wjcc.v3.i5.430","article-title":"Tick-borne encephalitis, A review of epidemiology.; clinical characteristics and management","volume":"3","author":"Strle","year":"2015","journal-title":"World J. 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