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The copper(II) complexes were prepared by the reaction of CuCl2\u00b72H2O or CuBr2 with ligands 1 and 2 in methanol solution. The copper(I) complexes were prepared by the reaction of Cu[(CH3CN)4]PF6 and 1,3,5-triaza-7-phosphaadamantane (PTA) or triphenylphosphine with LOMe and L2OMe in acetonitrile solution. Synchrotron radiation-based complementary techniques (XPS, NEXAFS, and XAS) were used to investigate the electronic and molecular structures of the complexes and the local structure around copper ions in selected Cu(I) and Cu(II) coordination compounds. All Cu(I) and Cu(II) complexes showed a significant in vitro antitumor activity, proving to be more effective than the reference drug cisplatin in a panel of human cancer cell lines, and were able to overcome cisplatin resistance. Noticeably, Cu complexes appeared much more effective than cisplatin in 3D spheroid cultures. Mechanistic studies revealed that the antitumor potential did not correlate with cellular accumulation but was consistent with intracellular targeting of PDI, ER stress, and paraptotic cell death induction.<\/jats:p>","DOI":"10.3390\/ijms23169397","type":"journal-article","created":{"date-parts":[[2022,8,21]],"date-time":"2022-08-21T22:23:13Z","timestamp":1661120593000},"page":"9397","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Exploring the Antitumor Potential of Copper Complexes Based on Ester Derivatives of Bis(pyrazol-1-yl)acetate Ligands"],"prefix":"10.3390","volume":"23","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5020-1730","authenticated-orcid":false,"given":"Maura","family":"Pellei","sequence":"first","affiliation":[{"name":"School of Science and Technology, Chemistry Division, University of Camerino, Via S. 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Res. Rev."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"329","DOI":"10.1016\/j.ccr.2014.08.002","article-title":"Noble metals in medicine: Latest advances","volume":"284","author":"Medici","year":"2015","journal-title":"Coord. Chem. Rev."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"9741","DOI":"10.1039\/D1CS00317H","article-title":"Chemical design principles of next-generation antiviral surface coatings","volume":"50","author":"Wang","year":"2021","journal-title":"Chem. Soc. Rev."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1459","DOI":"10.1039\/C5MT00149H","article-title":"Targeting copper in cancer therapy: \u2018Copper That Cancer\u2019","volume":"7","author":"Denoyer","year":"2015","journal-title":"Metallomics"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1497","DOI":"10.1039\/C5MT00163C","article-title":"Homoleptic phosphino copper(i) complexes with in vitro and in vivo dual cytotoxic and anti-angiogenic activity","volume":"7","author":"Gandin","year":"2015","journal-title":"Metallomics"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1007\/s10863-015-9640-x","article-title":"Antineoplastic copper coordinated complexes (Casiopeinas) uncouple oxidative phosphorylation and induce mitochondrial permeability transition in cardiac mitochondria and cardiomyocytes","volume":"48","author":"Castillo","year":"2016","journal-title":"J. Bioenerg. Biomembr."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"13936","DOI":"10.1038\/s41598-017-13698-1","article-title":"Therapeutic potential of the phosphino Cu(I) complex (HydroCuP) in the treatment of solid tumors","volume":"7","author":"Gandin","year":"2017","journal-title":"Sci. Rep."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1016\/j.cbpa.2016.12.011","article-title":"Developing drugs targeting transition metal homeostasis","volume":"37","author":"Weekley","year":"2017","journal-title":"Curr. Opin. Chem. Biol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"10758","DOI":"10.1039\/C7DT01955F","article-title":"A Perspective\u2014Can copper complexes be developed as a novel class of therapeutics?","volume":"46","author":"Wehbe","year":"2017","journal-title":"Dalton Trans."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1386","DOI":"10.1038\/s41467-019-09376-7","article-title":"X-ray structures of the high-affinity copper transporter Ctr1","volume":"10","author":"Ren","year":"2019","journal-title":"Nat. Commun."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"815","DOI":"10.1021\/cr400135x","article-title":"Advances in Copper Complexes as Anticancer Agents","volume":"114","author":"Santini","year":"2014","journal-title":"Chem. 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Sci., 21.","DOI":"10.3390\/ijms21113965"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"111134","DOI":"10.1016\/j.jinorgbio.2020.111134","article-title":"Anticancer potency of copper(II) complexes of thiosemicarbazones","volume":"210","author":"Singh","year":"2020","journal-title":"J. Inorg. Biochem."},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Lelievre, P., Sancey, L., Coll, J.L., Deniaud, A., and Busser, B. (2020). The Multifaceted Roles of Copper in Cancer: A Trace Metal Element with Dysregulated Metabolism, but Also a Target or a Bullet for Therapy. Cancers, 12.","DOI":"10.20944\/preprints202010.0353.v1"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"3201","DOI":"10.1039\/C5DT03919C","article-title":"Metal-based drugs that break the rules","volume":"45","author":"Allardyce","year":"2016","journal-title":"Dalton Trans."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"15584","DOI":"10.3390\/molecules191015584","article-title":"Cellular Transport Mechanisms of Cytotoxic Metallodrugs: An Overview beyond Cisplatin","volume":"19","author":"Spreckelmeyer","year":"2014","journal-title":"Molecules"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.ica.2016.01.006","article-title":"Current and future potential of metallo drugs: Revisiting DNA-binding of metal containing molecules and their diverse mechanism of action","volume":"444","author":"Zaki","year":"2016","journal-title":"Inorg. Chim. Acta"},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Molinaro, C., Martoriati, A., Pelinski, L., and Cailliau, K. (2020). Copper Complexes as Anticancer Agents Targeting Topoisomerases I and II. Cancers, 12.","DOI":"10.3390\/cancers12102863"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"9877","DOI":"10.1039\/c1dt10486a","article-title":"Nitroimidazole and glucosamine conjugated heteroscorpionate ligands and related copper(II) complexes. Syntheses, biological activity and XAS studies","volume":"40","author":"Pellei","year":"2011","journal-title":"Dalton Trans."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"174","DOI":"10.1016\/j.poly.2012.08.073","article-title":"Synchrotron radiation X-ray absorption spectroscopic studies in solution and electrochemistry of a nitroimidazole conjugated heteroscorpionate copper(II) complex","volume":"48","author":"Giorgetti","year":"2012","journal-title":"Polyhedron"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"11878","DOI":"10.1039\/C8NJ01763H","article-title":"Novel antitumor copper(ii) complexes designed to act through synergistic mechanisms of action, due to the presence of an NMDA receptor ligand and copper in the same chemical entity","volume":"42","author":"Morelli","year":"2018","journal-title":"N. J. Chem."},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Pellei, M., Bagnarelli, L., Luciani, L., Del Bello, F., Giorgioni, G., Piergentili, A., Quaglia, W., De Franco, M., Gandin, V., and Marzano, C. (2020). Synthesis and Cytotoxic Activity Evaluation of New Cu(I) Complexes of Bis(pyrazol-1-yl) Acetate Ligands Functionalized with an NMDA Receptor Antagonist. Int. J. Mol. Sci., 21.","DOI":"10.3390\/ijms21072616"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"4919","DOI":"10.1021\/acs.inorgchem.1c03658","article-title":"Cu(I) and Cu(II) Complexes Based on Lonidamine-Conjugated Ligands Designed to Promote Synergistic Antitumor Effects","volume":"61","author":"Pellei","year":"2022","journal-title":"Inorg. Chem."},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Schiesaro, I., Venditti, I., Pellei, M., Santini, C., Bagnarelli, L., Iucci, G., Battocchio, C., and Meneghini, C. (2021). Metal Coordination Core in Copper(II) Complexes Investigated by XAFS. Synchrotron Radiation Science and Applications, Springer. Springer Proceedings in Physics.","DOI":"10.1007\/978-3-030-72005-6_13"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"15622","DOI":"10.1039\/D0DT02952A","article-title":"Development of new and efficient copper(II) complexes of hexyl bis(pyrazolyl)acetate ligands as catalysts for allylic oxidation","volume":"49","author":"Gabrielli","year":"2020","journal-title":"Dalton Trans."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"234","DOI":"10.1016\/0009-2614(71)85038-8","article-title":"Molecular core binding energies for some five membered ring heterocycles as determined by X-ray photoelectron spectroscopy","volume":"9","author":"Clark","year":"1971","journal-title":"Chem. Phys. Lett."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"307","DOI":"10.1016\/j.chemphys.2003.10.024","article-title":"Interface formation between C60 and diethynyl-Zn-porphyrinato investigated by SR-induced photoelectron and near-edge X-ray absorption (NEXAFS) spectroscopies","volume":"297","author":"Polzonetti","year":"2004","journal-title":"Chem. Phys."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1338","DOI":"10.1016\/j.msec.2006.06.014","article-title":"Dinuclear Pt and Pd complexes with metalloporphyrin bridges: A NEXAFS study of the electronic structure and self-assembling properties","volume":"27","author":"Battocchio","year":"2007","journal-title":"Mater. Sci. Eng. C"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1002\/sia.740060504","article-title":"The adsorption of diethyldithiophosphate on cuprous sulphide","volume":"6","author":"Mielczarski","year":"1984","journal-title":"Surf. Interface Anal."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1016\/0368-2048(83)85005-1","article-title":"An ESCA investigation of some copper complexes","volume":"32","author":"Folkesson","year":"1983","journal-title":"J. Electron. Spectrosc. Relat. Phenom."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"1147","DOI":"10.1021\/ac60344a010","article-title":"Photoelectron spectrometric confirmation of the oxidation state of copper in so-called secondary copper(II) dithizonate","volume":"46","author":"Brand","year":"1974","journal-title":"Anal. Chem."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"767","DOI":"10.1039\/dt9800000767","article-title":"X-ray photoelectron spectra of some gold compounds","volume":"5","author":"McNeillie","year":"1980","journal-title":"J. Chem. Soc. Dalton Trans."},{"key":"ref_35","unstructured":"National Institute of Standards and Technology (2022, March 01). NIST X-ray Photoelectron Spectroscopy Database, Version 4.1, Available online: http:\/\/srdata.nist.gov\/xps\/."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"621","DOI":"10.1103\/RevModPhys.72.621","article-title":"Theoretical approaches to X-ray absorption fine structure","volume":"72","author":"Rehr","year":"2000","journal-title":"Rev. Mod. Phys."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"1204","DOI":"10.1103\/PhysRevLett.27.1204","article-title":"New technique for investigating noncrystalline structures: Fourier analysis of the extended X-ray\u2014Absorption fine structure","volume":"27","author":"Sayers","year":"1971","journal-title":"Phys. Rev. Lett."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"7565","DOI":"10.1103\/PhysRevB.58.7565","article-title":"Real-space multiple-scattering calculation and interpretation of X-ray-absorption near-edge structure","volume":"58","author":"Ankudinov","year":"1998","journal-title":"Phys. Rev. B"},{"key":"ref_39","first-page":"1895","article-title":"Mitochondrial Defects in cis-Diamminedichloroplatinum(II)-resistant Human Ovarian Carcinoma Cells","volume":"52","author":"Andrews","year":"1992","journal-title":"Cancer Res."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1186\/s13045-020-00931-0","article-title":"Modeling neoplastic disease with spheroids and organoids","volume":"13","author":"Zanoni","year":"2020","journal-title":"J. Hematol. Oncol."},{"key":"ref_41","unstructured":"Balakrishna, M.S. (2019). Phosphine\u2013copper(I) complexes as anticancer agents: Design, synthesis, and physicochemical characterization. Part I. Copper(I) Chemistry of Phosphines, Functionalized Phosphines and Phosphorus Heterocycles, Elsevier. Chapter 3."},{"key":"ref_42","unstructured":"Balakrishna, M.S. (2019). Phosphine copper(I) complexes as anticancer agents: Biological characterization. Part II. Copper(I) Chemistry of Phosphines, Functionalized Phosphines and Phosphorus Heterocycles, Elsevier. Chapter 4."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"140","DOI":"10.1016\/j.ejmech.2017.01.031","article-title":"Anti-proliferative effects of copper(II) complexes with hydroxyquinoline-thiosemicarbazone ligands","volume":"128","author":"Rogolino","year":"2017","journal-title":"Eur. J. Med. Chem."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"395","DOI":"10.1089\/ars.2019.7854","article-title":"High Copper Complex Stability and Slow Reduction Kinetics as Key Parameters for Improved Activity, Paraptosis Induction, and Impact on Drug-Resistant Cells of Anticancer Thiosemicarbazones","volume":"33","author":"Hager","year":"2020","journal-title":"Antioxid. Redox Signal."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"1052","DOI":"10.1038\/s41419-018-1102-z","article-title":"The thiosemicarbazone Me2NNMe2 induces paraptosis by disrupting the ER thiol redox homeostasis based on protein disulfide isomerase inhibition","volume":"9","author":"Hager","year":"2018","journal-title":"Cell Death Dis."},{"key":"ref_46","doi-asserted-by":"crossref","unstructured":"Fu, J., Gao, J., Liang, Z., and Yang, D. (2021). PDI-Regulated Disulfide Bond Formation in Protein Folding and Biomolecular Assembly. Molecules, 26.","DOI":"10.3390\/molecules26010171"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"78","DOI":"10.3389\/fonc.2017.00078","article-title":"Endoplasmic Reticulum Stress, Unfolded Protein Response, and Cancer Cell Fate","volume":"7","author":"Corazzari","year":"2017","journal-title":"Front. Oncol."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1016\/S0022-328X(01)00648-9","article-title":"Bis(pyrazol-1-yl)acetates as tripodal \u201cscorpionate\u201d ligands in transition metal carbonyl chemistry: Syntheses, structures and reactivity of manganese and rhenium carbonyl complexes of the type [LM(CO)3] (L = bpza, bdmpza)","volume":"626","author":"Burzlaff","year":"2001","journal-title":"J. Organomet. Chem."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"521","DOI":"10.1002\/1099-0682(200102)2001:2<521::AID-EJIC521>3.0.CO;2-Q","article-title":"Monoanionic N,N,O-scorpionate ligands and their iron(II) and zinc(II) complexes: Models for mononuclear active sites of non-heme iron oxidases and zinc enzymes","volume":"2","author":"Beck","year":"2001","journal-title":"Eur. J. Inorg. Chem."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"4045","DOI":"10.1021\/ic050193x","article-title":"Novel rhenium(V) oxo complexes containing bis(pyrazol-1-yl)acetate and bis(pyrazol-1-yl) sulfonate as tripodal N,N,O-heteroscorplonate ligands","volume":"44","author":"Porchia","year":"2005","journal-title":"Inorg. Chem."},{"key":"ref_51","first-page":"4989","article-title":"Synthesis and transition metal complexes of novel N,N,O scorpionate ligands suitable for solid phase immobilisation","volume":"24","author":"Haas","year":"2006","journal-title":"Eur. J. Inorg. Chem."},{"key":"ref_52","unstructured":"Moulder, J.F., Stickle, W.F., Sobol, P.E., and Bomben, K.D. (1996). Handbook of X-ray Photoelectron Spectroscopy, Perkin-Elmer Corporation, Physical Electronics Division."},{"key":"ref_53","doi-asserted-by":"crossref","unstructured":"St\u00f6hr, J. (1992). NEXAFS Spectroscopy, Springer. [1st ed.].","DOI":"10.1007\/978-3-662-02853-7"},{"key":"ref_54","first-page":"012043","article-title":"Novel XAFS capabilities at ELETTRA synchrotron light source","volume":"190","author":"Aquilanti","year":"2009","journal-title":"J. Phys.: Conf. Ser."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1016\/j.nimb.2012.05.027","article-title":"ESTRA-FitEXA: A software package for EXAFS data analysis","volume":"285","author":"Meneghini","year":"2012","journal-title":"Nucl. Instrum. Methods Phys. Res. Sect. B Beam Interact. Mater. At."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"710","DOI":"10.1016\/j.freeradbiomed.2009.05.027","article-title":"Treatment of human cancer cells with selenite or tellurite in combination with auranofin enhances cell death due to redox shift","volume":"47","author":"Rigobello","year":"2009","journal-title":"Free Radic. Biol. Med."}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/23\/16\/9397\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T00:12:47Z","timestamp":1760141567000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/23\/16\/9397"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,8,20]]},"references-count":56,"journal-issue":{"issue":"16","published-online":{"date-parts":[[2022,8]]}},"alternative-id":["ijms23169397"],"URL":"https:\/\/doi.org\/10.3390\/ijms23169397","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,8,20]]}}}