{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T04:48:04Z","timestamp":1787028484452,"version":"build-2736575974"},"reference-count":47,"publisher":"MDPI AG","issue":"17","license":[{"start":{"date-parts":[[2022,9,2]],"date-time":"2022-09-02T00:00:00Z","timestamp":1662076800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Tri-Service General Hospital, Taiwan","award":["TSGH-D-111074"],"award-info":[{"award-number":["TSGH-D-111074"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Increasing reports on the significance of dietary patterns in reproduction have arisen from both animal and human studies, suggesting an interactive association between nutrition and male fertility. The aim of this study was to investigate the effects of curcumin supplementation on low-carbohydrate-diet-induced metabolic dysfunction, testicular antioxidant capacity, apoptosis, inflammation and spermatogenesis in male mice. Male C57BL\/6 mice were fed a normal diet (AIN-93M group, n = 12) and a low-carbohydrate diet for 12 weeks (LC group, fed with low-carbohydrate diet, n = 48), and mice randomly chosen from the LC group were later fed their original diet (LC group, n = 12). This diet was changed to AIN-93M feed (LC\/AIN-93M group, n = 12), a ketogenic diet (LC\/KD group, n = 12), or a ketogenic diet treated with curcumin supplementation for the final 6 weeks (LC\/KDCu group, n = 12). A poor sperm morphology and mean testicular biopsy score (MTBS) were observed in the LC and LC\/KD groups, but they were eliminated by the normal diet or ketogenic diet with curcumin. The LC group exhibited a lower testicular testosterone level and a lower 17\u03b2-HSD activity and protein expression. This also enhanced apoptosis protein expressions in testis tissue, including Bax\/BCl2, cleaved caspase 3, PARP and NF-\u03baB. Meanwhile, we found a statistically significant increase in lipid peroxidation and decreased superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase levels in the LC group. Our study indicated that a replacement of a normal diet or ketogenic diet supplemented with curcumin attenuated poor semen quality and reduced testosterone levels by the LC diet by reducing oxidative stress.<\/jats:p>","DOI":"10.3390\/ijms231710009","type":"journal-article","created":{"date-parts":[[2022,9,6]],"date-time":"2022-09-06T05:17:23Z","timestamp":1662441443000},"page":"10009","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Curcumin Remedies Testicular Function and Spermatogenesis in Male Mice with Low-Carbohydrate-Diet-Induced Metabolic Dysfunction"],"prefix":"10.3390","volume":"23","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7214-8766","authenticated-orcid":false,"given":"Chih-Wei","family":"Tsao","sequence":"first","affiliation":[{"name":"Division of Urology, Department of Surgery, Tri-Service General Hospital, National Defense Medical Center, Taipei 114202, Taiwan"},{"name":"Division of Experimental Surgery Center, Department of Surgery, Tri-Service General Hospital, National Defense Medical Center, Taipei 114202, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pei-Shan","family":"Ke","sequence":"additional","affiliation":[{"name":"Department of Nutritional Science, Fu Jen Catholic University, New Taipei City 242304, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3733-4269","authenticated-orcid":false,"given":"Hsin-Yi","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Nutritional Science, Fu Jen Catholic University, New Taipei City 242304, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ting-Chia","family":"Chang","sequence":"additional","affiliation":[{"name":"Department of Nutritional Science, Fu Jen Catholic University, New Taipei City 242304, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chin-Yu","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Nutritional Science, Fu Jen Catholic University, New Taipei City 242304, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,9,2]]},"reference":[{"key":"ref_1","first-page":"641","article-title":"Male infertility-The other side of the equation","volume":"46","author":"Katz","year":"2017","journal-title":"Aust. 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