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In a previous study, which was carried out in a murine model of PE that was induced by NG-nitro-L-arginine methyl ester (L-NAME), we observed that the intravenous administration of fibroblast growth factor 2 (FGF2) had a hypotensive effect, improved the placental weight gain and attenuated the fetal growth restriction, and the morphological findings that were induced by L-NAME in the evaluated tissues were less severe. In this study, we aimed to determine the effect of FGF2 administration on the placental gene expression of the vascular endothelial growth factor (VEGFA), VEGF receptor 2 (VEGFR2), placental growth factor, endoglin (ENG), superoxide dismutase 1 (SOD1), catalase (CAT), thioredoxin (TXN), tumor protein P53 (P53), BCL2 apoptosis regulator, Fas cell surface death receptor (FAS), and caspase 3, in a Sprague Dawley rat PE model, which was induced by L-NAME. The gene expression was determined by a real-time polymerase chain reaction using SYBR green. Taking the vehicle or the L-NAME group as a reference, there was an under expression of placental VEGFA, VEGFR2, ENG, P53, FAS, SOD1, CAT, and TXN genes in the group of L-NAME + FGF2 (p &lt; 0.05). The administration of FGF2 in the murine PE-like model that was induced by L-NAME reduced the effects that were generated by proteinuria and the increased BP, as well as the response of the expression of genes that participate in angiogenesis, apoptosis, and OS. These results have generated valuable information regarding the identification of molecular targets for PE and provide new insights for understanding PE pathogenesis.<\/jats:p>","DOI":"10.3390\/ijms231710129","type":"journal-article","created":{"date-parts":[[2022,9,6]],"date-time":"2022-09-06T05:17:23Z","timestamp":1662441443000},"page":"10129","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Evaluation of the Effect of the Fibroblast Growth Factor Type 2 (FGF-2) Administration on Placental Gene Expression in a Murine Model of Preeclampsia Induced by L-NAME"],"prefix":"10.3390","volume":"23","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1478-9068","authenticated-orcid":false,"given":"Margarita L","family":"Martinez-Fierro","sequence":"first","affiliation":[{"name":"Molecular Medicine Laboratory, Unidad Academica de Medicina Humana y Ciencias de la Salud, Universidad Autonoma de Zacatecas, Carretera Zacatecas-Guadalajara Km. 6, Ejido la Escondida, Zacatecas 98160, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6307-1696","authenticated-orcid":false,"given":"Idalia","family":"Garza-Veloz","sequence":"additional","affiliation":[{"name":"Molecular Medicine Laboratory, Unidad Academica de Medicina Humana y Ciencias de la Salud, Universidad Autonoma de Zacatecas, Carretera Zacatecas-Guadalajara Km. 6, Ejido la Escondida, Zacatecas 98160, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Eugenia","family":"Casta\u00f1eda-Lopez","sequence":"additional","affiliation":[{"name":"Molecular Medicine Laboratory, Unidad Academica de Medicina Humana y Ciencias de la Salud, Universidad Autonoma de Zacatecas, Carretera Zacatecas-Guadalajara Km. 6, Ejido la Escondida, Zacatecas 98160, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dorothy","family":"Wasike","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, Comenius University Bratislava, \u0160pit\u00e1lska 24, 81372 Bratislava, Slovakia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claudia","family":"Castruita-De la Rosa","sequence":"additional","affiliation":[{"name":"Molecular Medicine Laboratory, Unidad Academica de Medicina Humana y Ciencias de la Salud, Universidad Autonoma de Zacatecas, Carretera Zacatecas-Guadalajara Km. 6, Ejido la Escondida, Zacatecas 98160, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5988-4168","authenticated-orcid":false,"given":"Iram Pablo","family":"Rodriguez-Sanchez","sequence":"additional","affiliation":[{"name":"Laboratorio de Fisiologia Molecular y Estructural, Facultad de Ciencias Biologicas, Universidad Autonoma de Nuevo Leon, Monterrey 66450, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9848-862X","authenticated-orcid":false,"given":"Ivan","family":"Delgado-Enciso","sequence":"additional","affiliation":[{"name":"School of Medicine, University of Colima, Colima 28040, Mexico"},{"name":"Colima State Health Services, Cancerology State Institute, Colima 28040, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jose","family":"Flores-Mendoza","sequence":"additional","affiliation":[{"name":"Molecular Medicine Laboratory, Unidad Academica de Medicina Humana y Ciencias de la Salud, Universidad Autonoma de Zacatecas, Carretera Zacatecas-Guadalajara Km. 6, Ejido la Escondida, Zacatecas 98160, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,9,4]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1690","DOI":"10.1016\/j.jacc.2020.08.014","article-title":"Preeclampsia-Pathophysiology and Clinical Presentations: JACC State-of-the-Art Review","volume":"76","author":"Ives","year":"2020","journal-title":"J. Am. Coll. Cardiol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"803","DOI":"10.1530\/REP-10-0294","article-title":"Remodelling at the maternal-fetal interface: Relevance to human pregnancy disorders","volume":"140","author":"Cartwright","year":"2010","journal-title":"Reproduction"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"293271","DOI":"10.1155\/2015\/293271","article-title":"Oxidative Stress in Placenta: Health and Diseases","volume":"2015","author":"Wu","year":"2015","journal-title":"BioMed Res. Int."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1016\/j.semnephrol.2010.10.004","article-title":"Angiogenic factors and preeclampsia","volume":"31","author":"Maynard","year":"2011","journal-title":"Semin. Nephrol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"158","DOI":"10.1097\/BCR.0b013e3181c7ed82","article-title":"Molecular mediators of angiogenesis","volume":"31","author":"Ucuzian","year":"2010","journal-title":"J. Burn Care Res. Off. Publ. Am. Burn Assoc."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"434","DOI":"10.1002\/biot.201200199","article-title":"Perivascular cells in blood vessel regeneration","volume":"8","author":"Wanjare","year":"2013","journal-title":"Biotechnol. J."},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Poto, R., Cristinziano, L., Modestino, L., de Paulis, A., Marone, G., Loffredo, S., Galdiero, M.R., and Varricchi, G. (2022). Neutrophil Extracellular Traps, Angiogenesis and Cancer. Biomedicines, 10.","DOI":"10.3390\/biomedicines10020431"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"814543","DOI":"10.1155\/2015\/814543","article-title":"Angiogenesis in the placenta: The role of reactive oxygen species signaling","volume":"2015","author":"Pereira","year":"2015","journal-title":"BioMed Res. Int."},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Munaut, C., Lorquet, S., Pequeux, C., Coulon, C., Le Goarant, J., Chantraine, F., Noel, A., Goffin, F., Tsatsaris, V., and Subtil, D. (2012). Differential expression of Vegfr-2 and its soluble form in preeclampsia. PLoS ONE, 7.","DOI":"10.1371\/journal.pone.0033475"},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Silvestro, S., Calcaterra, V., Pelizzo, G., Bramanti, P., and Mazzon, E. (2020). Prenatal Hypoxia and Placental Oxidative Stress: Insights from Animal Models to Clinical Evidences. Antioxidants, 9.","DOI":"10.3390\/antiox9050414"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1117","DOI":"10.1177\/1947601911423654","article-title":"Hypoxia-induced angiogenesis: Good and evil","volume":"2","author":"Krock","year":"2011","journal-title":"Genes Cancer"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"663044","DOI":"10.3389\/fphar.2021.663044","article-title":"Fibroblast Growth Factor Type 2 (FGF2) Administration Attenuated the Clinical Manifestations of Preeclampsia in a Murine Model Induced by L-NAME","volume":"12","year":"2021","journal-title":"Front. Pharmacol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1016\/j.atherosclerosis.2007.12.012","article-title":"VEGF function for upregulation of endogenous PlGF expression during FGF-2-mediated therapeutic angiogenesis","volume":"200","author":"Fujii","year":"2008","journal-title":"Atherosclerosis"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"58","DOI":"10.1016\/j.ijcard.2014.10.168","article-title":"Plasma cancer biomarker multiplex screening and the risk of subsequent preeclampsia","volume":"179","year":"2015","journal-title":"Int. J. Cardiol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"313","DOI":"10.1089\/jir.2008.0027","article-title":"Monocyte chemoattractant protein-1 (MCP-1): An overview","volume":"29","author":"Deshmane","year":"2009","journal-title":"J. Interferon Cytokine Res."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"6130","DOI":"10.1158\/1078-0432.CCR-11-0659","article-title":"Beyond VEGF: Inhibition of the fibroblast growth factor pathway and antiangiogenesis","volume":"17","author":"Lieu","year":"2011","journal-title":"Clin. Cancer Res."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"6730","DOI":"10.1158\/1078-0432.CCR-13-0081","article-title":"Ang-2-VEGF-A CrossMab, a novel bispecific human IgG1 antibody blocking VEGF-A and Ang-2 functions simultaneously, mediates potent antitumor, antiangiogenic, and antimetastatic efficacy","volume":"19","author":"Kienast","year":"2013","journal-title":"Clin. Cancer Res."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1016\/j.ejogrb.2014.08.021","article-title":"The optimization of a chronic nitric oxide synthase (NOS) inhibition model of pre-eclampsia by evaluating physiological changes","volume":"182","author":"Baijnath","year":"2014","journal-title":"Eur. J. Obstet. Gynecol. Reprod. Biol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1016\/j.ejphar.2016.12.010","article-title":"The effects of sildenafil citrate on uterine angiogenic status and serum inflammatory markers in an L-NAME rat model of pre-eclampsia","volume":"795","author":"Soobryan","year":"2017","journal-title":"Eur. J. Pharmacol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1433","DOI":"10.1111\/j.1476-5381.1996.tb15557.x","article-title":"Inhibition of nitric oxide synthesis by NG-nitro-L-arginine methyl ester (L-NAME): Requirement for bioactivation to the free acid, NG-nitro-L-arginine","volume":"118","author":"Pfeiffer","year":"1996","journal-title":"Br. J. Pharmacol."},{"key":"ref_21","first-page":"504","article-title":"Management of hypertensive crises in the elderly","volume":"15","author":"Alshami","year":"2018","journal-title":"J. Geriatr. Cardiol. JGC"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"160","DOI":"10.1161\/HYPERTENSIONAHA.117.10268","article-title":"Blood Pressure Control by a Secreted FGFBP1 (Fibroblast Growth Factor-Binding Protein)","volume":"71","author":"Tassi","year":"2018","journal-title":"Hypertension"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1097\/01.ogx.0000253301.55009.ac","article-title":"Clinical significance of proteinuria in pregnancy","volume":"62","author":"Airoldi","year":"2007","journal-title":"Obstet. Gynecol. Surv."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"609","DOI":"10.1007\/s00404-012-2354-y","article-title":"Effect of epidural block on 24-hour urine protein in pregnant rat models with preeclampsia","volume":"286","author":"Dong","year":"2012","journal-title":"Arch. Gynecol. Obstet."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"e20201061","DOI":"10.1590\/0034-7167-2020-1061","article-title":"Family functionality and burden of family caregivers of users with mental disorders","volume":"74","author":"Andrade","year":"2021","journal-title":"Rev. Bras. Enferm."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"477","DOI":"10.1016\/j.tice.2014.08.004","article-title":"Oxidative stress and apoptosis in preeclampsia","volume":"46","author":"Can","year":"2014","journal-title":"Tissue Cell"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"659","DOI":"10.1161\/01.ATV.20.3.659","article-title":"Nitric oxide induces the synthesis of vascular endothelial growth factor by rat vascular smooth muscle cells","volume":"20","author":"Dulak","year":"2000","journal-title":"Arterioscler. Thromb. Vasc. Biol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"37679","DOI":"10.1074\/jbc.274.53.37679","article-title":"Endogenous endothelial cell nitric-oxide synthase modulates apoptosis in cultured breast cancer cells and is transcriptionally regulated by p53","volume":"274","author":"Mortensen","year":"1999","journal-title":"J. Biol. Chem."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"783","DOI":"10.3906\/sag-1503-75","article-title":"NF-kappaBeta and SOD expression in preeclamptic placentas","volume":"46","year":"2016","journal-title":"Turk. J. Med. Sci."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"566-e1","DOI":"10.1016\/j.ajog.2008.04.020","article-title":"Differential placental gene expression in preeclampsia","volume":"199","author":"Enquobahrie","year":"2008","journal-title":"Am. J. Obstet. Gynecol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1093\/molehr\/gal011","article-title":"Gene expression profiling of human placentas from preeclamptic and normotensive pregnancies","volume":"12","author":"Hansson","year":"2006","journal-title":"Mol. Hum. Reprod."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"3179670","DOI":"10.1155\/2017\/3179670","article-title":"Assessment of Serum Vascular Endothelial Growth Factor Levels in Pregnancy-Induced Hypertension Patients","volume":"2017","author":"Tandon","year":"2017","journal-title":"J. Pregnancy"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"971","DOI":"10.1271\/bbb.120923","article-title":"Nitric oxide induces vascular endothelial growth factor expression in the rat placenta in vivo and in vitro","volume":"77","author":"Abe","year":"2013","journal-title":"Biosci. Biotechnol. Biochem."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"2939","DOI":"10.1038\/s41598-020-59853-z","article-title":"Activated FGF2 signaling pathway in tumor vasculature is essential for acquired resistance to anti-VEGF therapy","volume":"10","author":"Ichikawa","year":"2020","journal-title":"Sci. Rep."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"499","DOI":"10.1038\/cdd.2009.152","article-title":"VEGF-dependent tumor angiogenesis requires inverse and reciprocal regulation of VEGFR1 and VEGFR2","volume":"17","author":"Zhang","year":"2010","journal-title":"Cell Death Differ."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"1659","DOI":"10.1083\/jcb.141.7.1659","article-title":"Fibroblast growth factor-2 (FGF-2) induces vascular endothelial growth factor (VEGF) expression in the endothelial cells of forming capillaries: An autocrine mechanism contributing to angiogenesis","volume":"141","author":"Seghezzi","year":"1998","journal-title":"J. Cell Biol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"27","DOI":"10.3164\/jcbn.12-71","article-title":"Thioredoxin-1 and oxidative stress status in pregnant women at early third trimester of pregnancy: Relation to maternal and neonatal characteristics","volume":"52","author":"Nakatsukasa","year":"2013","journal-title":"J. Clin. Biochem. Nutr."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1016\/j.ijgo.2015.03.001","article-title":"Regional differences in the placental levels of oxidative stress markers in pre-eclampsia","volume":"129","author":"Sahay","year":"2015","journal-title":"Int. J. Gynaecol. Obstet. Off. Organ. Int. Fed. Gynaecol. Obstet."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"1433","DOI":"10.1177\/002215549704501012","article-title":"Differential localization of superoxide dismutase isoforms in placental villous tissue of normotensive, pre-eclamptic, and intrauterine growth-restricted pregnancies","volume":"45","author":"Myatt","year":"1997","journal-title":"J. Histochem. Cytochem."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"392","DOI":"10.1016\/j.placenta.2010.02.007","article-title":"Effects of labor on placental expression of superoxide dismutases in preeclampsia","volume":"31","author":"Roland","year":"2010","journal-title":"Placenta"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1177\/107155769600300404","article-title":"Antioxidant activities and mRNA expression of superoxide dismutase, catalase, and glutathione peroxidase in normal and preeclamptic placentas","volume":"3","author":"Wang","year":"1996","journal-title":"J. Soc. Gynecol. Investig."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1111\/j.1479-828X.2006.00532.x","article-title":"Endogenous anti-oxidants in pregnancy and preeclampsia","volume":"46","author":"Perkins","year":"2006","journal-title":"Aust. N. Z. J. Obstet. Gynaecol."},{"key":"ref_43","first-page":"1228","article-title":"Catalase activity in normal and preeclamptic placentas","volume":"72","author":"Wiktor","year":"2001","journal-title":"Ginekol. Pol."},{"key":"ref_44","first-page":"629","article-title":"Alterations of antioxidant enzymes in preeclampsia","volume":"3","author":"Krishna","year":"2015","journal-title":"IJRMS"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"84","DOI":"10.1016\/j.freeradbiomed.2015.06.017","article-title":"Regulation of catalase expression in healthy and cancerous cells","volume":"87","author":"Glorieux","year":"2015","journal-title":"Free Radic. Biol. Med."},{"key":"ref_46","first-page":"1679","article-title":"Fibrogrowth factor-2 protects against acute lung injury by activating the PI3K\/Akt signaling pathway","volume":"34","author":"Tang","year":"2020","journal-title":"J. Biol. Regul. Homeost. Agents"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"2476","DOI":"10.1016\/j.bbagen.2015.09.010","article-title":"Nitric oxide and the thioredoxin system: A complex interplay in redox regulation","volume":"1850","author":"Benhar","year":"2015","journal-title":"Biochim. Biophys. Acta"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"603","DOI":"10.1053\/plac.2000.0554","article-title":"Decreased expression of thioredoxin and glutaredoxin in placentae from pregnancies with pre-eclampsia and intrauterine growth restriction","volume":"21","author":"Sahlin","year":"2000","journal-title":"Placenta"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"191","DOI":"10.3892\/etm.2013.1399","article-title":"Apoptosis and p53 expression in the placental villi of females with unexplained recurrent spontaneous abortion","volume":"7","author":"Wei","year":"2014","journal-title":"Exp. Ther. Med."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"3758","DOI":"10.4161\/cc.10.21.17946","article-title":"The tumor suppressor p53 induces expression of the pregnancy-supporting human chorionic gonadotropin (hCG) CGB7 gene","volume":"10","author":"Sohr","year":"2011","journal-title":"Cell Cycle"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1093\/molehr\/gah006","article-title":"First trimester trophoblast cells secrete Fas ligand which induces immune cell apoptosis","volume":"10","author":"Abrahams","year":"2004","journal-title":"Mol. Hum. Reprod."},{"key":"ref_52","first-page":"178","article-title":"The role of apoptosis in preeclampsia","volume":"7","author":"Levy","year":"2005","journal-title":"Isr Med. Assoc. J."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"R412","DOI":"10.1152\/ajpregu.00880.2005","article-title":"Uteroplacental insufficiency increases p53 phosphorylation without triggering the p53-MDM2 functional circuit response in the IUGR rat kidney","volume":"291","author":"Baserga","year":"2006","journal-title":"Am. J. Physiol. Regul. Integr. Comp. Physiol."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"447","DOI":"10.1093\/molehr\/8.5.447","article-title":"Hormonal regulation of apoptosis and the Fas and Fas ligand system in human endometrial cells","volume":"8","author":"Song","year":"2002","journal-title":"Mol. Hum. Reprod."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"1055","DOI":"10.3892\/etm.2021.10489","article-title":"Fas regulates the apoptosis and migration of trophoblast cells by targeting NF-\u03baB","volume":"22","author":"Lan","year":"2021","journal-title":"Exp. Ther. Med."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"471","DOI":"10.1515\/JPM.2005.085","article-title":"The role of Fas mediated apoptosis in preeclampsia","volume":"33","author":"Neale","year":"2005","journal-title":"J. Perinat. Med."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"1101","DOI":"10.1038\/nprot.2008.73","article-title":"Analyzing real-time PCR data by the comparative C(T) method","volume":"3","author":"Schmittgen","year":"2008","journal-title":"Nat. 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