{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T12:44:07Z","timestamp":1791895447287,"version":"4.3.4"},"reference-count":53,"publisher":"MDPI AG","issue":"17","license":[{"start":{"date-parts":[[2022,8,26]],"date-time":"2022-08-26T00:00:00Z","timestamp":1661472000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Generalitat de Catalunya","award":["PID2019-108578RB-I00"],"award-info":[{"award-number":["PID2019-108578RB-I00"]}]},{"name":"CIBERER\/ISCIII","award":["PID2019-108578RB-I00"],"award-info":[{"award-number":["PID2019-108578RB-I00"]}]},{"name":"Ministerio de Ciencia e Innovaci\u00f3n\/FEDER","award":["PID2019-108578RB-I00"],"award-info":[{"award-number":["PID2019-108578RB-I00"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Activation of NF-\u03baB transcription factor is strictly regulated to accurately direct cellular processes including inflammation, immunity, and cell survival. In the retina, the modulation of the NF-\u03baB pathway is essential to prevent excessive inflammatory responses, which plays a pivotal role in many retinal neurodegenerative diseases, such as age-related macular degeneration (AMD), diabetic retinopathy (DR), and inherited retinal dystrophies (IRDs). A critical cytokine mediating inflammatory responses in retinal cells is tumor necrosis factor-alpha (TNF\u03b1), leading to the activation of several transductional pathways, including NF-\u03baB. However, the multiple factors orchestrating the appropriate regulation of NF-\u03baB in retinal cells still remain unclear. The present study explores how the ubiquitin-specific protease 48 (USP48) downregulation impacts the stability and transcriptional activity of NF-\u03baB\/p65 in retinal pigment epithelium (RPE), at both basal conditions and following TNF\u03b1 stimulation. We described that USP48 downregulation stabilizes p65. Notably, the accumulation of p65 is mainly detectable in the nuclear compartment and it is accompanied by an increased NF-\u03baB transcriptional activity. These results delineate a novel role of USP48 in negatively regulating NF-\u03baB in retinal cells, providing new opportunities for therapeutic intervention in retinal pathologies.<\/jats:p>","DOI":"10.3390\/ijms23179682","type":"journal-article","created":{"date-parts":[[2022,8,29]],"date-time":"2022-08-29T01:39:19Z","timestamp":1661737159000},"page":"9682","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Ubiquitin Specific Protease USP48 Destabilizes NF-\u03baB\/p65 in Retinal Pigment Epithelium Cells"],"prefix":"10.3390","volume":"23","author":[{"given":"Serena","family":"Mirra","sequence":"first","affiliation":[{"name":"Department of Genetics, Microbiology and Statistics, Universitat de Barcelona, Avda. Diagonal 643,  08028 Barcelona, Spain"},{"name":"CIBERER, Instituto de Salud Carlos III, 28029 Madrid, Spain"},{"name":"Institut de Biomedicina-Institut de Recerca Sant Joan de D\u00e9u (IBUB-IRSJD), Universitat de Barcelona, 08028 Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3534-704X","authenticated-orcid":false,"given":"Laura","family":"S\u00e1nchez-Bellver","sequence":"additional","affiliation":[{"name":"Department of Genetics, Microbiology and Statistics, Universitat de Barcelona, Avda. Diagonal 643,  08028 Barcelona, Spain"},{"name":"CIBERER, Instituto de Salud Carlos III, 28029 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carmela","family":"Casale","sequence":"additional","affiliation":[{"name":"Institute of Genetics and Biophysics, \u2018Adriano Buzzati-Traverso\u2019 (CNR), Via Pietro Castellino 111, 80131 Naples, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alessandra","family":"Pescatore","sequence":"additional","affiliation":[{"name":"Institute of Genetics and Biophysics, \u2018Adriano Buzzati-Traverso\u2019 (CNR), Via Pietro Castellino 111, 80131 Naples, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7941-983X","authenticated-orcid":false,"given":"Gemma","family":"Marfany","sequence":"additional","affiliation":[{"name":"Department of Genetics, Microbiology and Statistics, Universitat de Barcelona, Avda. 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