{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,10]],"date-time":"2026-07-10T22:10:37Z","timestamp":1783721437265,"version":"3.55.0"},"reference-count":62,"publisher":"MDPI AG","issue":"17","license":[{"start":{"date-parts":[[2022,8,27]],"date-time":"2022-08-27T00:00:00Z","timestamp":1661558400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"the Ministry of Science and Higher Education of the Russian Federation","award":["0729-2020-0061"],"award-info":[{"award-number":["0729-2020-0061"]}]},{"name":"Molecular Biology and Neurophysiology","award":["0729-2020-0061"],"award-info":[{"award-number":["0729-2020-0061"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Currently, the role of the neurotrophic factors BDNF and GDNF in maintaining the brain\u2019s resistance to the damaging effects of hypoxia and functional recovery of neural networks after exposure to damaging factors are actively studied. The assessment of the effect of an increase in the level of these neurotrophic factors in brain tissues using genetic engineering methods on the resistance of laboratory animals to hypoxia may pave the way for the future clinical use of neurotrophic factors BDNF and GDNF in the treatment of hypoxic damage. This study aimed to evaluate the antihypoxic and neuroprotective properties of BDNF and GDNF expression level increase using adeno-associated viral vectors in modeling hypoxia in vivo. To achieve overexpression of neurotrophic factors in the central nervous system\u2019s cells, viral constructs were injected into the brain ventricles of newborn male C57Bl6 (P0) mice. Acute hypobaric hypoxia was modeled on the 30th day after the injection of viral vectors. Survival, cognitive, and mnestic functions in the late post-hypoxic period were tested. Evaluation of growth and weight characteristics and the neurological status of animals showed that the overexpression of neurotrophic factors does not affect the development of mice. It was found that the use of adeno-associated viral vectors increased the survival rate of male mice under hypoxic conditions. The present study indicates that the neurotrophic factors\u2019 overexpression, induced by the specially developed viral constructs carrying the BDNF and GDNF genes, is a prospective neuroprotection method, increasing the survival rate of animals after hypoxic injury.<\/jats:p>","DOI":"10.3390\/ijms23179733","type":"journal-article","created":{"date-parts":[[2022,8,29]],"date-time":"2022-08-29T01:39:19Z","timestamp":1661737159000},"page":"9733","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Overexpression of Brain- and Glial Cell Line-Derived Neurotrophic Factors Is Neuroprotective in an Animal Model of Acute Hypobaric Hypoxia"],"prefix":"10.3390","volume":"23","author":[{"given":"Maria S.","family":"Gavrish","sequence":"first","affiliation":[{"name":"Institute of Biology and Biomedicine, Lobachevsky State University of Nizhny Novgorod, 23 Gagarina Ave., 603022 Nizhny Novgorod, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark D.","family":"Urazov","sequence":"additional","affiliation":[{"name":"Institute of Biology and Biomedicine, Lobachevsky State University of Nizhny Novgorod, 23 Gagarina Ave., 603022 Nizhny Novgorod, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4463-5035","authenticated-orcid":false,"given":"Tatiana A.","family":"Mishchenko","sequence":"additional","affiliation":[{"name":"Institute of Biology and Biomedicine, Lobachevsky State University of Nizhny Novgorod, 23 Gagarina Ave., 603022 Nizhny Novgorod, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Victoria D.","family":"Turubanova","sequence":"additional","affiliation":[{"name":"Institute of Biology and Biomedicine, Lobachevsky State University of Nizhny Novgorod, 23 Gagarina Ave., 603022 Nizhny Novgorod, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ekaterina A.","family":"Epifanova","sequence":"additional","affiliation":[{"name":"Institute of Cell Biology and Neurobiology, Charit\u00e9-Universit\u00e4tsmedizin Berlin, Charit\u00e9platz 1, 10117 Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7731-1331","authenticated-orcid":false,"given":"Victoria G.","family":"Krut\u2019","sequence":"additional","affiliation":[{"name":"Institute of Biology and Biomedicine, Lobachevsky State University of Nizhny Novgorod, 23 Gagarina Ave., 603022 Nizhny Novgorod, Russia"},{"name":"Scientific Center for Genetics and Life Sciences, Sirius University of Science and Technology, 354340 Sochi, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexey A.","family":"Babaev","sequence":"additional","affiliation":[{"name":"Institute of Biology and Biomedicine, Lobachevsky State University of Nizhny Novgorod, 23 Gagarina Ave., 603022 Nizhny Novgorod, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9759-6477","authenticated-orcid":false,"given":"Maria V.","family":"Vedunova","sequence":"additional","affiliation":[{"name":"Institute of Biology and Biomedicine, Lobachevsky State University of Nizhny Novgorod, 23 Gagarina Ave., 603022 Nizhny Novgorod, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1703-2618","authenticated-orcid":false,"given":"Elena V.","family":"Mitroshina","sequence":"additional","affiliation":[{"name":"Institute of Biology and Biomedicine, Lobachevsky State University of Nizhny Novgorod, 23 Gagarina Ave., 603022 Nizhny Novgorod, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,8,27]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"910","DOI":"10.1097\/WNR.0000000000000860","article-title":"Knockdown of Long Noncoding Antisense RNA Brain-Derived Neurotrophic Factor Attenuates Hypoxia\/Reoxygenation-Induced Nerve Cell Apoptosis through the BDNF\u2013TrkB\u2013PI3K\/Akt Signaling Pathway","volume":"28","author":"Zhong","year":"2017","journal-title":"NeuroReport"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1036907","DOI":"10.1155\/2019\/1036907","article-title":"Intracellular Neuroprotective Mechanisms in Neuron-Glial Networks Mediated by Glial Cell Line-Derived Neurotrophic Factor","volume":"2019","author":"Mitroshina","year":"2019","journal-title":"Oxidative Med. 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