{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T04:40:39Z","timestamp":1760244039287,"version":"build-2065373602"},"reference-count":66,"publisher":"MDPI AG","issue":"17","license":[{"start":{"date-parts":[[2022,8,30]],"date-time":"2022-08-30T00:00:00Z","timestamp":1661817600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2017YFD0600104","PAPD","KYCX20_0872"],"award-info":[{"award-number":["2017YFD0600104","PAPD","KYCX20_0872"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Priority Academic Program Development of the Jiangsu Higher Education Institutions","award":["2017YFD0600104","PAPD","KYCX20_0872"],"award-info":[{"award-number":["2017YFD0600104","PAPD","KYCX20_0872"]}]},{"name":"Postgraduate Research &amp; Practice Innovation Program of Jiangsu Province","award":["2017YFD0600104","PAPD","KYCX20_0872"],"award-info":[{"award-number":["2017YFD0600104","PAPD","KYCX20_0872"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Iron deficiency causes chlorosis and growth inhibition in Cinnamomum camphora, an important landscaping tree species. Siderophores produced by plant growth-promoting rhizobacteria have been widely reported to play an indispensable role in plant iron nutrition. However, little to date has been determined about how microbial siderophores promote plant iron absorption. In this study, multidisciplinary approaches, including physiological, biochemical and transcriptome methods, were used to investigate the role of deferoxamine (DFO) in regulating Fe availability in C. camphora seedlings. Our results showed that DFO supplementation significantly increased the Fe2+ content, SPAD value and ferric-chelate reductase (FCR) activity in plants, suggesting its beneficial effect under Fe deficiency. This DFO-driven amelioration of Fe deficiency was further supported by the improvement of photosynthesis. Intriguingly, DFO treatment activated the metabolic pathway of glutathione (GSH) synthesis, and exogenous spraying reduced glutathione and also alleviated chlorosis in C. camphora. In addition, the expression of some Fe acquisition and transport-related genes, including CcbHLH, CcFRO6, CcIRT2, CcNramp5, CcOPT3 and CcVIT4, was significantly upregulated by DFO treatment. Collectively, our data demonstrated an effective, economical and feasible organic iron-complexing agent for iron-deficient camphor trees and provided new insights into the mechanism by which siderophores promote iron absorption in plants.<\/jats:p>","DOI":"10.3390\/ijms23179854","type":"journal-article","created":{"date-parts":[[2022,8,31]],"date-time":"2022-08-31T00:13:56Z","timestamp":1661904836000},"page":"9854","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Physiological and Transcriptome Analyses Revealed the Mechanism by Which Deferoxamine Promotes Iron Absorption in Cinnamomum camphora"],"prefix":"10.3390","volume":"23","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6365-1677","authenticated-orcid":false,"given":"Wei-Liang","family":"Kong","sequence":"first","affiliation":[{"name":"Co-Innovation Center for Sustainable Forestry in Southern China, College of Forestry, Nanjing Forestry University, Nanjing 210037, China"},{"name":"Jiangsu Key Laboratory for Prevention and Management of Invasive Species, Nanjing Forestry University, Nanjing 210037, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1889-1388","authenticated-orcid":false,"given":"Tong-Yue","family":"Wen","sequence":"additional","affiliation":[{"name":"Co-Innovation Center for Sustainable Forestry in Southern China, College of Forestry, Nanjing Forestry University, Nanjing 210037, China"},{"name":"Jiangsu Key Laboratory for Prevention and Management of Invasive Species, Nanjing Forestry University, Nanjing 210037, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ya-Hui","family":"Wang","sequence":"additional","affiliation":[{"name":"Co-Innovation Center for Sustainable Forestry in Southern China, College of Forestry, Nanjing Forestry University, Nanjing 210037, China"},{"name":"Jiangsu Key Laboratory for Prevention and Management of Invasive Species, Nanjing Forestry University, Nanjing 210037, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0309-1676","authenticated-orcid":false,"given":"Xiao-Qin","family":"Wu","sequence":"additional","affiliation":[{"name":"Co-Innovation Center for Sustainable Forestry in Southern China, College of Forestry, Nanjing Forestry University, Nanjing 210037, China"},{"name":"Jiangsu Key Laboratory for Prevention and Management of Invasive Species, Nanjing Forestry University, Nanjing 210037, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,8,30]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"3011","DOI":"10.3390\/molecules24163011","article-title":"A Mass Spectrometry-Based Study Shows that Volatiles Emitted by Arthrobacter agilis UMCV2 Increase the Content of Brassinosteroids in Medicago truncatula in Response to Iron Deficiency Stress","volume":"24","author":"Winkler","year":"2019","journal-title":"Molecules"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1869","DOI":"10.1111\/pce.13998","article-title":"Low nitrate alleviates iron deficiency by regulating iron homeostasis in apple","volume":"44","author":"Sun","year":"2021","journal-title":"Plant Cell Environ."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"413","DOI":"10.1180\/claymin.2010.045.4.413","article-title":"Mossbauer spectroscopy of clays, soils and their mineral constituents","volume":"45","author":"Murad","year":"2010","journal-title":"Clay Miner."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"596","DOI":"10.3389\/fpls.2015.00596","article-title":"Augmenting iron accumulation in cassava by the beneficial soil bacterium Bacillus subtilis (GBO3)","volume":"6","author":"Freitas","year":"2015","journal-title":"Front. Plant Sci."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"104497","DOI":"10.1016\/j.still.2019.104497","article-title":"Spatial variations of aggregate-associated humic substance in heavy-textured soils along a climatic gradient","volume":"197","author":"Wei","year":"2020","journal-title":"Soil Tillage Res."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"538","DOI":"10.1016\/j.tplants.2017.03.008","article-title":"Mobilization of Iron by Plant-Borne Coumarins","volume":"22","author":"Tsai","year":"2017","journal-title":"Trends Plant Sci."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"144","DOI":"10.1016\/j.scienta.2017.02.043","article-title":"Root plant growth promoting rhizobacteria inoculations increase ferric chelate reductase (FC-R) activity and Fe nutrition in pear under calcareous soil conditions","volume":"219","author":"Ipek","year":"2017","journal-title":"Sci. Hortic."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"611","DOI":"10.1007\/s00344-015-9495-8","article-title":"Volatile organic compounds produced by the rhizobacterium Arthrobacter agilis UMCV2 modulate Sorghum bicolor (Strategy II Plant) morphogenesis and SbFRO1 transcription in vitro","volume":"34","author":"Santoyo","year":"2015","journal-title":"J. Plant Growth Regul."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"2167","DOI":"10.1104\/pp.113.233585","article-title":"Scavenging iron: A novel mechanism of plant immunity activation by Microbial Siderophores","volume":"164","author":"Aznar","year":"2014","journal-title":"Plant Physiol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1080\/17429145.2017.1407000","article-title":"Improved iron acquisition of Astragalus sinicus under low iron-availability conditions by soil-borne bacteria Burkholderia cepacia","volume":"13","author":"Zhou","year":"2018","journal-title":"J. Plant Interact."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"656","DOI":"10.1016\/j.bjm.2017.02.006","article-title":"Promotion of iron nutrition and growth on peanut by Paenibacillus illinoisensis and Bacillus sp. strains in calcareous soil","volume":"48","author":"Liu","year":"2017","journal-title":"Braz. J. Microbiol."},{"key":"ref_12","first-page":"S115","article-title":"Involvement of Trichoderma asperellum strain T6 in regulating iron acquisition in plants","volume":"541","author":"Zhao","year":"2014","journal-title":"J. Basic Microbiol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1016\/j.apsoil.2011.10.015","article-title":"Engineering heterologous iron siderophore complex utilization in rhizobia: Effect on growth of peanut and pigeon pea plants","volume":"53","author":"Arif","year":"2012","journal-title":"Appl. Soil Ecol."},{"key":"ref_14","first-page":"2299","article-title":"The microbial siderophore desferrioxamine B inhibits Fe and Zn uptake in three spring wheat genotypes grown in Fe-deficient nutrient solution","volume":"44","author":"Sadrarhami","year":"2021","journal-title":"J. Plant Nutr."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"736","DOI":"10.1080\/01904167.2016.1262409","article-title":"Effects of two new siderophore-producing rhizobacteria on growth and iron content of maize and canola plants","volume":"40","author":"Ghavami","year":"2017","journal-title":"J. Plant Nutr."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"686","DOI":"10.1016\/j.jbiosc.2012.12.018","article-title":"Efficacy of a bacterial siderophore, pyoverdine, to supply iron to Solanum lycopersicum plants","volume":"115","author":"Nagata","year":"2013","journal-title":"J. Biosci. Bioeng."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"409","DOI":"10.1016\/S1002-0160(19)60810-6","article-title":"Siderophore-producing rhizobacteria as a promising tool for empowering plants to cope with iron limitation in saline soils: A. review","volume":"29","author":"Ferreira","year":"2019","journal-title":"Pedosphere"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s13568-019-0796-3","article-title":"Comparison of five bacterial strains producing siderophores with ability to chelate iron under alkaline conditions","volume":"9","author":"Ferreira","year":"2019","journal-title":"AMB Express"},{"key":"ref_19","first-page":"e01746-19","article-title":"Siderophore-mediated iron acquisition enhances resistance to oxidative and aromatic compound stress in Cupriavidus necator JMP134","volume":"85","author":"Li","year":"2019","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Morcillo, R., V\u00edlchez, J., Zhang, S., Kaushal, R., He, D., Zi, H., Liu, R., Niehaus, K., Handa, A., and Zhang, H. (2021). Plant transcriptome reprograming and bacterial extracellular metabolites underlying tomato drought resistance triggered by a beneficial soil bacteria. Metabolites, 11.","DOI":"10.3390\/metabo11060369"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1111\/jph.12960","article-title":"The early transcriptional responses in rose induced by Bacillus velezensis CLA178 and Agrobacterium tumefaciens C58","volume":"169","author":"Chen","year":"2021","journal-title":"J. Phytopathol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"584174","DOI":"10.3389\/fmicb.2020.584174","article-title":"Transcriptome profiles reveal the growth-promoting mechanisms of Paenibacillus polymyxa YC0136 on Tobacco (Nicotiana tabacum L.)","volume":"11","author":"Liu","year":"2020","journal-title":"Front. Microbiol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"4402","DOI":"10.4238\/2012.October.9.7","article-title":"Identification and expression analysis of multiple FRO gene copies in Medicago truncatula","volume":"11","author":"Santoyo","year":"2012","journal-title":"Genet. Mol. Res."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"709","DOI":"10.1016\/j.plantsci.2009.02.011","article-title":"Iron uptake mechanisms in plants: Functions of the FRO family of ferric reductases","volume":"176","author":"Jeong","year":"2009","journal-title":"Plant Sci."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"100","DOI":"10.3389\/fpls.2014.00100","article-title":"Connolly, The diverse roles of FRO family metalloreductases in iron and copper homeostasis","volume":"5","author":"Jain","year":"2014","journal-title":"Front. Plant Sci."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"100422","DOI":"10.1016\/j.scp.2021.100422","article-title":"Re-use of Cinnamomum camphora natural dye generated wastewater for sustainable UV protective and antioxidant finishing of wool fabric: Effect of Fe(II) sulfate","volume":"21","author":"Rather","year":"2021","journal-title":"Sustain. Chem. Pharm."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"223","DOI":"10.2298\/ABS210320017T","article-title":"Antibacterial, antioxidant, cytotoxic effects and GC-MS analysis of mangrove-derived Streptomyces achromogenes TCH4 extract","volume":"73","author":"Tangjitjaroenkun","year":"2021","journal-title":"Arch. Biol. Sci."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"1550","DOI":"10.1021\/acs.jnatprod.1c00061","article-title":"Aromatic polyketides and hydroxamate siderophores from a marine-algae-derived streptomyces species","volume":"84","author":"Xia","year":"2021","journal-title":"J. Nat. Prod."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1007\/s11104-004-5212-2","article-title":"The use of microbial siderophores for foliar iron application studies","volume":"272","author":"Ebert","year":"2005","journal-title":"Plant Soil"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1007\/s10534-004-5773-9","article-title":"The determination of ferric iron in plants by HPLC using the microbial iron chelator desferrioxamine E","volume":"18","author":"Fernandez","year":"2005","journal-title":"Biometals"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"746","DOI":"10.1080\/15226514.2017.1284752","article-title":"Effects of citric acid and the siderophore desferrioxamine B (DFO-B) on the mobility of germanium and rare earth elements in soil and uptake in Phalaris arundinacea","volume":"19","author":"Wiche","year":"2017","journal-title":"Int. J. Phytoremediat."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"877","DOI":"10.1007\/s00344-019-10029-8","article-title":"Effects of Rahnella aquatilis JZ-GX1 on treat chlorosis induced by iron deficiency in Cinnamomum camphora","volume":"39","author":"Kong","year":"2020","journal-title":"J. Plant Growth Regul."},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Zhao, Y., Li, H., Sun, M., Liang, Z., Yu, F., Li, F., and Liu, S. (2021). Effects of soil aeration and fertilization practices on alleviating iron deficiency chlorosis in \u201cHuangguan\u201d pears grafted onto quince A in calcareous soils. Horticulturae, 7.","DOI":"10.3390\/horticulturae7070172"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1016\/j.plaphy.2019.06.035","article-title":"Compound repair effect of carbon dots and Fe2+ on iron deficiency in Cucumis melon L.","volume":"142","author":"Yang","year":"2019","journal-title":"Plant Physiol. Biochem."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1235","DOI":"10.1093\/treephys\/tpz055","article-title":"NRAMP1 promotes iron uptake at the late stage of iron deficiency in poplars","volume":"39","author":"Chen","year":"2019","journal-title":"Tree Physiol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"253","DOI":"10.1021\/acs.jafc.6b03274","article-title":"Effectiveness of iron ethylenediamine-N,N\u2032-bis(hydroxyphenylacetic) acid (o,o-EDDHA\/Fe3+) formulations with different ratios of meso and d,l-racemic isomers as iron fertilizers","volume":"65","year":"2017","journal-title":"J. Agric. Food Chem."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1007\/s11104-015-2759-z","article-title":"Effects of individual and combined metal foliar fertilisers on iron- and manganese-deficient Solanum lycopersicum plants","volume":"402","author":"Rios","year":"2016","journal-title":"Plant Soil"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"1498","DOI":"10.2134\/agronj2018.12.0770","article-title":"Solubility of ten iron fertilizers in eleven north american soils","volume":"111","author":"Shaddox","year":"2019","journal-title":"Agron. J."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"757","DOI":"10.1007\/s11284-018-1558-4","article-title":"The effect of different iron concentrations on lead accumulation in hydroponically grown Matthiola flavida Boiss","volume":"33","author":"Dehno","year":"2018","journal-title":"Ecol. Res."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"1687","DOI":"10.1104\/pp.109.138636","article-title":"Microbial siderophores exert a subtle role in arabidopsis during infection by manipulating the immune response and the iron status","volume":"150","author":"Dellagi","year":"2009","journal-title":"Plant Physiol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"991","DOI":"10.1038\/s41429-019-0217-5","article-title":"Nocardamin glucuronide, a new member of the ferrioxamine siderophores isolated from the ascamycin-producing strain Streptomyces sp. 80H647","volume":"72","author":"Lopez","year":"2019","journal-title":"J. Antibiot."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"146770","DOI":"10.1016\/j.scitotenv.2021.146770","article-title":"Well-to-wake analysis of switchgrass to jet fuel via a novel co-fermentation of sugars and CO2","volume":"782","author":"Pamula","year":"2021","journal-title":"Sci. Total Environ."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"1431","DOI":"10.1111\/tpj.15104","article-title":"Chromoplast differentiation in bell pepper (Capsicum annuum) fruits","volume":"105","author":"Agne","year":"2021","journal-title":"Plant J."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1111\/tpj.14319","article-title":"Electron transport pathways in isolated chromoplasts from Narcissus pseudonarcissus L.","volume":"99","author":"Grabsztunowicz","year":"2019","journal-title":"Plant J."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"110421","DOI":"10.1016\/j.scienta.2021.110421","article-title":"Exogenous hydrogen peroxide induces chilling tolerance in Phalaenopsis seedlings through glutathione-related antioxidant system","volume":"289","author":"Chen","year":"2021","journal-title":"Sci. Hortic."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"110360","DOI":"10.1016\/j.scienta.2021.110360","article-title":"Seed-priming with H2O2 alleviates subsequent salt stress by preventing ROS production and amplifying antioxidant defense in cauliflower seeds and seedlings","volume":"288","author":"Ellouzi","year":"2021","journal-title":"Sci. Hortic."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"104569","DOI":"10.1016\/j.envexpbot.2021.104569","article-title":"Elemental localisation and a reduced glutathione redox state protect seeds of the halophyte Suaeda maritima from salinity during over-wintering and germination","volume":"190","author":"Szymansky","year":"2021","journal-title":"Environ. Exp. Bot."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"1120","DOI":"10.3390\/genes12081120","article-title":"Expression of a truncated yeast Ccc1 vacuolar transporter increases the accumulation of endogenous iron","volume":"12","author":"Puig","year":"2021","journal-title":"Genes"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"1679","DOI":"10.1111\/pce.14000","article-title":"FIT and bHLH Ib transcription factors modulate iron and copper crosstalk in Arabidopsis","volume":"44","author":"Cai","year":"2021","journal-title":"Plant Cell Environ."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"13938","DOI":"10.1073\/pnas.212448699","article-title":"The tomato fer gene encoding a bHLH protein controls iron-uptake responses in roots","volume":"99","author":"Ling","year":"2002","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"260","DOI":"10.1016\/j.plaphy.2007.03.006","article-title":"FIT, the FER-like iron deficiency induced transcription factor in Arabidopsis","volume":"45","author":"Bauer","year":"2007","journal-title":"Plant Physiol. Biochem."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1016\/j.plaphy.2020.11.013","article-title":"Streptomyces hygroscopicus OsiSh-2-induced mitigation of Fe deficiency in rice plants","volume":"158","author":"Cao","year":"2021","journal-title":"Plant Physiol. Biochem."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"109205","DOI":"10.1016\/j.scienta.2020.109205","article-title":"Melatonin alleviates iron stress by improving iron homeostasis, antioxidant defense and secondary metabolism in cucumber","volume":"265","author":"Ahammed","year":"2020","journal-title":"Sci. Hortic."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"4839","DOI":"10.1093\/jxb\/erab159","article-title":"The tonoplast-localized transporter OsNRAMP2 is involved in iron homeostasis and affects seed germination in rice","volume":"72","author":"Li","year":"2021","journal-title":"J. Exp. Bot."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"2434","DOI":"10.1104\/pp.17.00672","article-title":"Wheat vacuolar iron transporter ta VIT2 transports Fe and Mn and is effective for biofortification","volume":"174","author":"Connorton","year":"2017","journal-title":"Plant Physiol."},{"key":"ref_56","doi-asserted-by":"crossref","unstructured":"Hu, Y.T., Ming, F., Chen, W.W., Yan, J.Y., Xu, Z.Y., Li, G.X., Xu, C.Y., Yang, J.L., and Zheng, S.J. (2012). TcOPT3, a member of oligopeptide transporters from the hyperaccumulator thlaspi caerulescens, is a novel Fe\/Zn\/Cd\/Cu transporter. PLoS ONE, 7.","DOI":"10.1371\/journal.pone.0038535"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"2467","DOI":"10.1016\/j.ygeno.2020.01.021","article-title":"Characterization of the pectin methyl-esterase gene family and its function in controlling pollen tube growth in pear (Pyrus bretschneideri)","volume":"112","author":"Tang","year":"2020","journal-title":"Genomics"},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"10746","DOI":"10.1038\/srep10746","article-title":"Elevation of NO production increases Fe immobilization in the Fe-deficiency roots apoplast by decreasing pectin methylation of cell wall","volume":"5","author":"Ye","year":"2015","journal-title":"Sci. Rep."},{"key":"ref_59","first-page":"117","article-title":"Transcriptome analysis of nanocellulose-Fe chelate correcting iron-deficiency chlorosis of pear","volume":"50","author":"Guo","year":"2021","journal-title":"J. Henan Agric. Sci."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"110376","DOI":"10.1016\/j.scienta.2021.110376","article-title":"Role of melatonin in improving leaf mineral content and growth of sweet corn seedlings under different soil salinity levels","volume":"288","author":"Sezer","year":"2021","journal-title":"Sci. Hortic."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1080\/01904169509364892","article-title":"Determination of iron chlorosis with extractable iron analysis in peach leaves","volume":"18","author":"Koseoglu","year":"1995","journal-title":"J. Plant Nutr."},{"key":"ref_62","first-page":"1347","article-title":"Application of improved NH4F masking method in determination of ferrous iron in plant tissues","volume":"51","author":"Ni","year":"2015","journal-title":"Plant Physiol. J."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"568","DOI":"10.1111\/j.1365-313X.2009.03803.x","article-title":"A soil bacterium regulates plant acquisition of iron via deficiency-inducible mechanisms","volume":"58","author":"Zhang","year":"2009","journal-title":"Plant J."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"2141","DOI":"10.1080\/01904167.2018.1482910","article-title":"Effect of plant growth promoting rhizobacteria on Fe acquisition in peach (Prunus persica L.) under calcareous soil conditions","volume":"41","author":"Aras","year":"2018","journal-title":"J. Plant Nutr."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"704000","DOI":"10.3389\/fpls.2021.704000","article-title":"Enhanced iron uptake in plants by volatile emissions of Rahnella aquatilis JZ-GX1","volume":"12","author":"Kong","year":"2021","journal-title":"Front. Plant Sci."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"1114","DOI":"10.3389\/fmicb.2020.01114","article-title":"Antifungal effects of volatile organic compounds produced by Rahnella aquatilis JZ-GX1 Against Colletotrichum gloeosporioides in Liriodendron chinense x tulipifera","volume":"11","author":"Kong","year":"2020","journal-title":"Front. Microbiol."}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/23\/17\/9854\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T00:20:15Z","timestamp":1760142015000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/23\/17\/9854"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,8,30]]},"references-count":66,"journal-issue":{"issue":"17","published-online":{"date-parts":[[2022,9]]}},"alternative-id":["ijms23179854"],"URL":"https:\/\/doi.org\/10.3390\/ijms23179854","relation":{},"ISSN":["1422-0067"],"issn-type":[{"type":"electronic","value":"1422-0067"}],"subject":[],"published":{"date-parts":[[2022,8,30]]}}}