{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,27]],"date-time":"2026-10-27T08:10:02Z","timestamp":1793088602468,"version":"4.5.0"},"reference-count":36,"publisher":"MDPI AG","issue":"17","license":[{"start":{"date-parts":[[2022,9,1]],"date-time":"2022-09-01T00:00:00Z","timestamp":1661990400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"SATT Ouest Valorisation","award":["#DV2541"],"award-info":[{"award-number":["#DV2541"]}]},{"name":"University of Angers","award":["#DV2541"],"award-info":[{"award-number":["#DV2541"]}]},{"name":"UC Louvain pole of Morphology","award":["#DV2541"],"award-info":[{"award-number":["#DV2541"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Osteogenesis imperfecta (OI) is a genetic disorder of connective tissue characterized by low bone mass and spontaneous fractures, as well as extra-skeletal manifestations, such as dental abnormalities, blue sclera, hearing loss and joint hypermobility. Tendon ruptures have been reported in OI patients. Here, we characterized the biomechanical, structural and tissue material properties of bone and tendon in 5-week-old female osteogenesis imperfecta mice (oim), a validated model of severe type III OI, and compared these data with age- and sex-matched WT littermates. Oim tendons were less rigid and less resistant than those of WT mice. They also presented a significantly higher rate of pentosidine, without significant modification of enzymatic crosslinking. The oim bones were less resistant and avulsion fractures were evident at high tendinous stress areas. Alterations of trabecular and cortical bone microarchitectures were noticed in young female oim. Bone tissue material properties were also modified, with a less mature and more mineralized matrix in association with lower collagen maturity. Our data suggest that the tendon-to-bone unit is affected in young oim mice, which could explain tendon ruptures and bone fragility observed in OI patients.<\/jats:p>","DOI":"10.3390\/ijms23179928","type":"journal-article","created":{"date-parts":[[2022,9,1]],"date-time":"2022-09-01T01:50:51Z","timestamp":1661997051000},"page":"9928","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["Biomechanical, Microstructural and Material Properties of Tendon and Bone in the Young Oim Mice Model of Osteogenesis Imperfecta"],"prefix":"10.3390","volume":"23","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1510-3515","authenticated-orcid":false,"given":"Antoine","family":"Chretien","sequence":"first","affiliation":[{"name":"Pole of Morphology, Institute of Experimental and Clinical Research (IREC), UCLouvain, 1200 Brussels, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8860-9026","authenticated-orcid":false,"given":"Malory","family":"Couchot","sequence":"additional","affiliation":[{"name":"Univ Angers, Nantes Universit\u00e9, Oniris, Inserm, RMeS, REGOS, SFR ICAT, F-49000 Angers, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1445-329X","authenticated-orcid":false,"given":"Guillaume","family":"Mabilleau","sequence":"additional","affiliation":[{"name":"Univ Angers, Nantes Universit\u00e9, Oniris, Inserm, RMeS, REGOS, SFR ICAT, F-49000 Angers, France"},{"name":"Department of Cell and Tissue Pathology, CHU Angers, F-49933 Angers, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0468-7076","authenticated-orcid":false,"given":"Catherine","family":"Behets","sequence":"additional","affiliation":[{"name":"Pole of Morphology, Institute of Experimental and Clinical Research (IREC), UCLouvain, 1200 Brussels, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,9,1]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1377","DOI":"10.1016\/S0140-6736(04)16051-0","article-title":"Osteogenesis imperfecta","volume":"363","author":"Rauch","year":"2004","journal-title":"Lancet"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1041","DOI":"10.1016\/S0140-6736(78)90763-8","article-title":"Classification of osteogenesis imperfect","volume":"1","author":"Sillence","year":"1978","journal-title":"Lancet"},{"key":"ref_3","first-page":"1470","article-title":"Osteogenesis imperfecta: Clinical diagnosis, nomenclature and severity assessment","volume":"164a","author":"Sillence","year":"2014","journal-title":"Am. J. Med. Genet. A"},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Nguyen, H.T.T., Vu, D.C., Nguyen, D.M., Dang, Q.D., Tran, V.K., Le, H., and Tong, S.M. (2021). Dentinogenesis Imperfecta and Caries in Osteogenesis Imperfecta among Vietnamese Children. Dent. J., 9.","DOI":"10.3390\/dj9050049"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1016\/j.metabol.2017.06.001","article-title":"Osteogenesis imperfecta\u2014A clinical update","volume":"80","author":"Tournis","year":"2018","journal-title":"Metabolism"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/s11914-016-0299-y","article-title":"Advances in the Classification and Treatment of Osteogenesis Imperfecta","volume":"14","author":"Thomas","year":"2016","journal-title":"Curr. Osteoporos. Rep."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"2515","DOI":"10.1016\/j.injury.2017.08.069","article-title":"Acute traumatic rupture of the patellar tendon in pediatric population: Case series and review of the literature","volume":"48","author":"Rosenfeld","year":"2017","journal-title":"Injury"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"327","DOI":"10.1007\/s00167-011-1595-9","article-title":"Distal patellar tendon avulsion fracture in a football player with osteogenesis imperfecta","volume":"20","author":"Jansen","year":"2012","journal-title":"Knee Surg. Sports Traumatol. Arthrosc."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"339","DOI":"10.1016\/j.jcot.2020.01.013","article-title":"Tibial-tubercle avulsion and patellar-tendon rupture in pre-pubertal child with osteogenesis imperfecta(OI): Case report and review of current treatment in OI","volume":"11","author":"Mehta","year":"2020","journal-title":"J. Clin. Orthop. Trauma"},{"key":"ref_10","first-page":"e15466","article-title":"Simultaneous Bilateral Patellar Tendon Rupture in a Patient With Osteogenesis Imperfecta","volume":"13","author":"Nasra","year":"2021","journal-title":"Cureus"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"333","DOI":"10.32098\/mltj.03.2014.12","article-title":"The enthesis: A review of the tendon-to-bone insertion","volume":"4","author":"Apostolakos","year":"2014","journal-title":"Muscles Ligaments Tendons J."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"84","DOI":"10.1016\/j.jdsr.2016.04.003","article-title":"Molecular characterization and function of tenomodulin, a marker of tendons and ligaments that integrate musculoskeletal components","volume":"52","author":"Shukunami","year":"2016","journal-title":"Jpn.Dent. Sci. Rev."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1007\/s00198-009-1066-z","article-title":"Collagen cross-links as a determinant of bone quality: A possible explanation for bone fragility in aging, osteoporosis, and diabetes mellitus","volume":"21","author":"Saito","year":"2010","journal-title":"Osteoporos. Int."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"101057","DOI":"10.1016\/j.bonr.2021.101057","article-title":"Osteogenesis imperfecta and sclerostin antibody\u2014Histological evaluation of the bone-tendon unit in the oim mouse","volume":"14","author":"Chretien","year":"2021","journal-title":"Bone Rep."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"172","DOI":"10.1007\/s002239900412","article-title":"Bone geometry and strength measurements in aging mice with the oim mutation","volume":"62","author":"McBride","year":"1998","journal-title":"Calcif. Tissue Int."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"494","DOI":"10.1007\/s00223-019-00655-5","article-title":"Sclerostin-Antibody Treatment Decreases Fracture Rates in Axial Skeleton and Improves the Skeletal Phenotype in Growing oim\/oim Mice","volume":"106","author":"Cardinal","year":"2020","journal-title":"Calcif. Tissue Int."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"264","DOI":"10.1359\/jbmr.1999.14.2.264","article-title":"The material basis for reduced mechanical properties in oim mice bones","volume":"14","author":"Camacho","year":"1999","journal-title":"J. Bone Miner. Res."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"453","DOI":"10.1016\/S8756-3282(01)00594-4","article-title":"Age- and genotype-dependence of bone material properties in the osteogenesis imperfecta murine model (oim)","volume":"29","author":"Grabner","year":"2001","journal-title":"Bone"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"705505","DOI":"10.3389\/fgene.2021.705505","article-title":"Gender-Related Impact of Sclerostin Antibody on Bone in the Osteogenesis Imperfecta Mouse","volume":"12","author":"Cardinal","year":"2021","journal-title":"Front. Genet."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"e63488","DOI":"10.7554\/eLife.63488","article-title":"Tendon and motor phenotypes in the Crtap(-\/-) mouse model of recessive osteogenesis imperfecta","volume":"10","author":"Grol","year":"2021","journal-title":"Elife"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"451","DOI":"10.1016\/j.matbio.2005.07.001","article-title":"Alpha 2(I) collagen deficient oim mice have altered biomechanical integrity, collagen content, and collagen crosslinking of their thoracic aorta","volume":"24","author":"Pfeiffer","year":"2005","journal-title":"Matrix Biol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1392","DOI":"10.1002\/jbmr.2172","article-title":"How tough is brittle bone? Investigating osteogenesis imperfecta in mouse bone","volume":"29","author":"Carriero","year":"2014","journal-title":"J. Bone Miner. Res."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1016\/j.jmbbm.2019.06.002","article-title":"Pentosidine correlates with nanomechanical properties of human jaw bone","volume":"98","author":"Kawamura","year":"2019","journal-title":"J. Mech. Behav. Biomed. Mater."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"1317","DOI":"10.1016\/j.bone.2012.03.007","article-title":"Ultra-structural defects cause low bone matrix stiffness despite high mineralization in osteogenesis imperfecta mice","volume":"50","author":"Vanleene","year":"2012","journal-title":"Bone"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1016\/j.biomaterials.2016.08.038","article-title":"Studies of chain substitution caused sub-fibril level differences in stiffness and ultrastructure of wildtype and oim\/oim collagen fibers using multifrequency-AFM and molecular modeling","volume":"107","author":"Li","year":"2016","journal-title":"Biomaterials"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"315","DOI":"10.1016\/j.bone.2015.07.024","article-title":"Raman spectral classification of mineral- and collagen-bound water\u2019s associations to elastic and post-yield mechanical properties of cortical bone","volume":"81","author":"Unal","year":"2015","journal-title":"Bone"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"314","DOI":"10.1016\/j.tripleo.2006.10.003","article-title":"Osteogenesis imperfecta","volume":"103","author":"Huber","year":"2007","journal-title":"Oral. Surg. Oral. Med. Oral. Pathol. Oral. Radiol. Endod."},{"key":"ref_28","first-page":"Cd005088","article-title":"Bisphosphonate therapy for osteogenesis imperfecta","volume":"10","author":"Dwan","year":"2016","journal-title":"Cochrane Database Syst. Rev."},{"key":"ref_29","first-page":"1","article-title":"Role of induced pluripotent stem cells (IPSCS) in bone tissue regeneration in dentistry: A narrative review","volume":"34","author":"Nagni","year":"2020","journal-title":"J. Biol. Regul. Homeost. Agents"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"337","DOI":"10.1007\/s11914-020-00594-3","article-title":"Stem Cell Therapy as a Treatment for Osteogenesis Imperfecta","volume":"18","year":"2020","journal-title":"Curr. Osteoporos. Rep."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"C149","DOI":"10.1152\/ajpcell.00241.2009","article-title":"Role of TRPC1 channel in skeletal muscle function","volume":"298","author":"Zanou","year":"2010","journal-title":"Am. J. Physiol.-Cell Physiol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"1363","DOI":"10.1002\/jbmr.4004","article-title":"Enteroendocrine K Cells Exert Complementary Effects to Control Bone Quality and Mass in Mice","volume":"35","author":"Gobron","year":"2020","journal-title":"J. Bone Miner. Res."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"1468","DOI":"10.1002\/jbmr.141","article-title":"Guidelines for assessment of bone microstructure in rodents using micro-computed tomography","volume":"25","author":"Bouxsein","year":"2010","journal-title":"J. Bone Miner. Res."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"228","DOI":"10.1016\/j.bone.2014.07.021","article-title":"Molecular spectroscopic identification of the water compartments in bone","volume":"67","author":"Unal","year":"2014","journal-title":"Bone"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"281","DOI":"10.1007\/s00223-020-00720-4","article-title":"Dapagliflozin and Liraglutide Therapies Rapidly Enhanced Bone Material Properties and Matrix Biomechanics at Bone Formation Site in a Type 2 Diabetic Mouse Model","volume":"107","author":"Mieczkowska","year":"2020","journal-title":"Calcif. Tissue Int."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"829","DOI":"10.1007\/s00216-009-3019-y","article-title":"Collagen types analysis and differentiation by FTIR spectroscopy","volume":"395","author":"Belbachir","year":"2009","journal-title":"Anal. Bioanal. Chem."}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/23\/17\/9928\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T00:21:28Z","timestamp":1760142088000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/23\/17\/9928"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,9,1]]},"references-count":36,"journal-issue":{"issue":"17","published-online":{"date-parts":[[2022,9]]}},"alternative-id":["ijms23179928"],"URL":"https:\/\/doi.org\/10.3390\/ijms23179928","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,9,1]]}}}