{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T02:18:22Z","timestamp":1775009902907,"version":"3.50.1"},"reference-count":32,"publisher":"MDPI AG","issue":"18","license":[{"start":{"date-parts":[[2022,9,6]],"date-time":"2022-09-06T00:00:00Z","timestamp":1662422400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"HELIOS Kliniken GmbH","award":["046315"],"award-info":[{"award-number":["046315"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Non-surgical bleeding (NSB) is one of the major clinical complications in patients under continuous-flow left ventricular assist device (LVAD) support. The increased shear stress leads to an altered platelet receptor composition. Whether these changes increase the risk for NSB is unclear. Thus, we compared the platelet receptor composition of patients with (bleeder group, n = 18) and without NSB (non-bleeder group, n = 18) prior to LVAD implantation. Blood samples were obtained prior to LVAD implantation and after bleeding complications in the post-implant period. Platelet receptor expression of GPIb\u03b1, GPIIb\/IIIa, P-selectin and CD63 as well as intra-platelet oxidative stress levels were quantified by flow cytometry. Bleeders and non-bleeders were comparable regarding clinical characteristics, von Willebrand factor diagnostics and the aggregation capacity before and after LVAD implantation (p &gt; 0.05). LVAD patients in the bleeder group suffered from gastrointestinal bleeding (33%; n = 6), epistaxis (22%; n = 4), hematuria or hematoma (17%; n = 3, respectively) and cerebral bleeding (11%; n = 2). Prior to LVAD implantation, a restricted surface expression of the platelet receptors P-selectin and GPIIb\/IIIa was observed in the bleeder group (P-selectin: 7.2 \u00b1 2.6%; GPIIb\/IIIa: 26,900 \u00b1 13,608 U) compared to non-bleeders (P-selectin: 12.4 \u00b1 8.1%, p = 0.02; GPIIb\/IIIa: 36,259 \u00b1 9914 U; p = 0.02). We hypothesized that the reduced platelet receptor expression of P-selectin and GPIIb\/IIIa prior to LVAD implantation may be linked to LVAD-related NSB.<\/jats:p>","DOI":"10.3390\/ijms231810252","type":"journal-article","created":{"date-parts":[[2022,9,6]],"date-time":"2022-09-06T20:52:51Z","timestamp":1662497571000},"page":"10252","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Decreased Platelet Specific Receptor Expression of P-Selectin and GPIIb\/IIIa Predict Future Non-Surgical Bleeding in Patients after Left Ventricular Assist Device Implantation"],"prefix":"10.3390","volume":"23","author":[{"given":"Kristin","family":"Klaeske","sequence":"first","affiliation":[{"name":"Department of Cardiac Surgery, Heart Center Leipzig, University of Leipzig, 04289 Leipzig, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4833-8014","authenticated-orcid":false,"given":"Anna L.","family":"Meyer","sequence":"additional","affiliation":[{"name":"Department of Cardiac Surgery, University Hospital Heidelberg, 69120 Heidelberg, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Diyar","family":"Saeed","sequence":"additional","affiliation":[{"name":"Department of Cardiac Surgery, Heart Center Leipzig, University of Leipzig, 04289 Leipzig, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sandra","family":"Eifert","sequence":"additional","affiliation":[{"name":"Department of Cardiac Surgery, Heart Center Leipzig, University of Leipzig, 04289 Leipzig, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7897-1462","authenticated-orcid":false,"given":"Khalil","family":"Jawad","sequence":"additional","affiliation":[{"name":"Department of Cardiac Surgery, Heart Center Leipzig, University of Leipzig, 04289 Leipzig, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Franz","family":"Sieg","sequence":"additional","affiliation":[{"name":"Department of Cardiac Surgery, Heart Center Leipzig, University of Leipzig, 04289 Leipzig, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Josephina","family":"Haunschild","sequence":"additional","affiliation":[{"name":"Department of Cardiac Surgery, Heart Center Leipzig, University of Leipzig, 04289 Leipzig, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael A.","family":"Borger","sequence":"additional","affiliation":[{"name":"Department of Cardiac Surgery, Heart Center Leipzig, University of Leipzig, 04289 Leipzig, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5503-9901","authenticated-orcid":false,"given":"Maja-Theresa","family":"Dieterlen","sequence":"additional","affiliation":[{"name":"Department of Cardiac Surgery, Heart Center Leipzig, University of Leipzig, 04289 Leipzig, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,9,6]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"233","DOI":"10.1136\/heartjnl-2020-317886","article-title":"Implantable left ventricular assist device: Indications, eligibility and current outcomes","volume":"108","author":"Bhagra","year":"2022","journal-title":"Heart"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1931","DOI":"10.1002\/ehf2.13899","article-title":"Prevalence, management, and outcomes of haemorrhagic events in left ventricular assist device recipients","volume":"9","author":"Pourtau","year":"2022","journal-title":"ESC Heart Fail."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"736","DOI":"10.1111\/aor.13456","article-title":"Evaluation of anticoagulation and nonsurgical major bleeding in recipients of continuous-flow left ventricular assist devices","volume":"43","author":"Veasey","year":"2019","journal-title":"Artif. 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