{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T11:46:41Z","timestamp":1772192801135,"version":"3.50.1"},"reference-count":30,"publisher":"MDPI AG","issue":"18","license":[{"start":{"date-parts":[[2022,9,7]],"date-time":"2022-09-07T00:00:00Z","timestamp":1662508800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Fondo de Investigaci\u00f3n en Salud IMSS","award":["FIS\/IMSS\/PROT\/PRIO\/18\/076"],"award-info":[{"award-number":["FIS\/IMSS\/PROT\/PRIO\/18\/076"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Chronic Myeloid Leukemia (CML) originates in a leukemic stem cell that resides in the bone marrow microenvironment, where they coexist with cellular and non-cellular elements. The vascular microenvironment has been identified as an important element in CML development since an increase in the vascularization has been suggested to be related with poor prognosis; also, using murine models, it has been reported that bone marrow endothelium can regulate the quiescence and proliferation of leukemic stem and progenitor cells. This observation, however, has not been evaluated in primary human cells. In this report, we used a co-culture of primitive (progenitor and stem) CML cells with endothelial colony forming cells (ECFC) as an in vitro model to evaluate the effects of the vascular microenvironment in the leukemic hematopoiesis. Our results show that this interaction allows the in vitro maintenance of primitive CML cells through an inflammatory microenvironment able to regulate the proliferation of progenitor cells and the permanence in a quiescent state of leukemic stem cells.<\/jats:p>","DOI":"10.3390\/ijms231810326","type":"journal-article","created":{"date-parts":[[2022,9,8]],"date-time":"2022-09-08T01:40:13Z","timestamp":1662601213000},"page":"10326","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Cell Contact with Endothelial Cells Favors the In Vitro Maintenance of Human Chronic Myeloid Leukemia Stem and Progenitor Cells"],"prefix":"10.3390","volume":"23","author":[{"given":"Patricia","family":"Torres-Barrera","sequence":"first","affiliation":[{"name":"Laboratorio de C\u00e9lulas Troncales Leuc\u00e9micas, Unidad de Investigaci\u00f3n M\u00e9dica en Enfermedades Oncol\u00f3gicas, CMN Siglo XXI, Instituto Mexicano del Seguro Social, CDMX 06725, Mexico"},{"name":"Posgrado en Ciencias Biol\u00f3gicas, UNAM, CDMX 04510, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3029-4241","authenticated-orcid":false,"given":"Dafne","family":"Moreno-Lorenzana","sequence":"additional","affiliation":[{"name":"CONACYT-Instituto Nacional de Pediatria, CDMX 04530, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jos\u00e9 Antonio","family":"Alvarado-Moreno","sequence":"additional","affiliation":[{"name":"Unidad de Investigaci\u00f3n M\u00e9dica en Trombosis Hemostasia y Aterogenesis, Instituto Mexicano del Seguro Social, CDMX 03100, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elena","family":"Garc\u00eda-Ruiz","sequence":"additional","affiliation":[{"name":"Departamento de Hematolog\u00eda, Hospital de Especialidades, CMN La Raza, Instituto Mexicano del Seguro Social, CDMX 02990, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cesar","family":"Lagunas","sequence":"additional","affiliation":[{"name":"Departamento de Cirug\u00edas de Cadera, Hospital General \u201cVilla Coapa\u201d Instituto Mexicano del Seguro Social, CDMX 14310, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hector","family":"Mayani","sequence":"additional","affiliation":[{"name":"Laboratorio de C\u00e9lulas Troncales Hematopoy\u00e9ticas, Unidad de Investigaci\u00f3n M\u00e9dica en Enfermedades Oncol\u00f3gicas, CMN Siglo XXI, Instituto Mexicano del Seguro Social, CDMX 06725, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4542-1023","authenticated-orcid":false,"given":"Antonieta","family":"Ch\u00e1vez-Gonz\u00e1lez","sequence":"additional","affiliation":[{"name":"Laboratorio de C\u00e9lulas Troncales Leuc\u00e9micas, Unidad de Investigaci\u00f3n M\u00e9dica en Enfermedades Oncol\u00f3gicas, CMN Siglo XXI, Instituto Mexicano del Seguro Social, CDMX 06725, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,9,7]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"290","DOI":"10.1038\/243290a0","article-title":"Letter: A new consistent chromosomal abnormality in chronic myelogenous leukaemia identified by quinacrine fluorescence and Giemsa staining","volume":"243","author":"Rowley","year":"1973","journal-title":"Nature"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"286","DOI":"10.1016\/j.leukres.2005.06.028","article-title":"Functional integrity in vitro of hematopoietic progenitor cells from patients with chronic myeloid leukemia that have achieved hematological remission after different therapeutic procedures","volume":"30","author":"Mayani","year":"2006","journal-title":"Leuk. 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