{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T07:26:57Z","timestamp":1788852417649,"version":"build-2803163510"},"reference-count":45,"publisher":"MDPI AG","issue":"18","license":[{"start":{"date-parts":[[2022,9,8]],"date-time":"2022-09-08T00:00:00Z","timestamp":1662595200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"NHC Key Laboratory of Male Reproduction and Genetics","award":["KF201906"],"award-info":[{"award-number":["KF201906"]}]},{"name":"NHC Key Laboratory of Male Reproduction and Genetics","award":["2021A1515220178"],"award-info":[{"award-number":["2021A1515220178"]}]},{"name":"Guangdong Basic and Applied Basic Research Foundation","award":["KF201906"],"award-info":[{"award-number":["KF201906"]}]},{"name":"Guangdong Basic and Applied Basic Research Foundation","award":["2021A1515220178"],"award-info":[{"award-number":["2021A1515220178"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>This study is to analyze the effect of C-type natriuretic peptide (CNP) on sperm motility of asthenozoospermia and explore the influence mechanism of CNP on the reproductive system and sperm motility. Our results showed that the concentration of CNP in asthenospermia patients\u2019 semen was lower than in normal people\u2019s. The motility of sperm could be improved markedly by CNP and 8-Br-cGMP, while the effect of CNP was inhibited by NPR-B antagonist and KT5823. In the asthenozoospermia mouse model induced by CTX, CNP injection could improve sperm motility in the epididymis, alleviate tissue damage in the testes and epididymis, and increase testosterone levels. The asthenospermia mouse model showed high activity of MDA and proinflammatory factors (TNF-\u03b1, IL-6), as well as low expression of antioxidants (SOD, GSH-Px, CAT) in the testis and epididymis, but this situation could be significantly ameliorated after being treated with CNP. Those studies indicated that the concentration of CNP in the semen of asthenospermia patients is lower than in normal people and could significantly promote sperm motility through the NPR-B\/cGMP pathway. In the asthenospermia mouse model induced by CTX, CNP can alleviate the damage of cyclophosphamide to the reproductive system and sperm motility. The mechanism may involve increasing testosterone and reducing ROS and proinflammatory factors to damage the tissue and sperm.<\/jats:p>","DOI":"10.3390\/ijms231810370","type":"journal-article","created":{"date-parts":[[2022,9,8]],"date-time":"2022-09-08T07:05:56Z","timestamp":1662620756000},"page":"10370","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["C-Type Natriuretic Peptide (CNP) Could Improve Sperm Motility and Reproductive Function of Asthenozoospermia"],"prefix":"10.3390","volume":"23","author":[{"given":"Na","family":"Li","sequence":"first","affiliation":[{"name":"Institute of Reproduction Health Research, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinyi","family":"Dong","sequence":"additional","affiliation":[{"name":"Institute of Reproduction Health Research, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sen","family":"Fu","sequence":"additional","affiliation":[{"name":"Institute of Reproduction Health Research, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoyan","family":"Wang","sequence":"additional","affiliation":[{"name":"Reproductive Center, Qingdao Women and Children\u2019s Hospital Affiliated to Qingdao University, Qingdao 266034, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huaibiao","family":"Li","sequence":"additional","affiliation":[{"name":"Institute of Reproduction Health Research, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ge","family":"Song","sequence":"additional","affiliation":[{"name":"NHC Key Laboratory of Male Reproduction and Genetics, Family Planning Research Institute of Guangdong Province, Guangzhou 510006, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Donghui","family":"Huang","sequence":"additional","affiliation":[{"name":"Institute of Reproduction Health Research, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China"},{"name":"Shenzhen Huazhong University of Science and Technology Research Institute, Huazhong University of Science and Technology, Shenzhen 518109, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,9,8]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"864","DOI":"10.1016\/j.fertnstert.2019.01.032","article-title":"Home testing for male factor infertility: A review of current options","volume":"111","author":"Kobori","year":"2019","journal-title":"Fertil. 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