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Therefore, it has developed specialized pathways to scavenge it from its host. In recent years, some proteins involved in the import of heme, such as LHR1 and LFLVCRB, have been identified, but relevant aspects regarding the process remain unknown. Here, we characterized the kinetics of the uptake of the heme analogue Zn(II) Mesoporphyrin IX (ZnMP) in Leishmania major promastigotes as a model of a parasite causing cutaneous leishmaniasis with special focus on the force that drives the process. We found that ZnMP uptake is an active, inducible, and pH-dependent process that does not require a plasma membrane proton gradient but requires the presence of the monovalent cations Na+ and\/or K+. In addition, we demonstrated that this parasite can efflux this porphyrin against a concentration gradient. We also found that ZnMP uptake differs among different dermotropic or viscerotropic Leishmania species and does not correlate with LHR1 or LFLVCRB expression levels. Finally, we showed that these transporters have only partially overlapping functions. Altogether, these findings contribute to a deeper understanding of an important process in the biology of this parasite.<\/jats:p>","DOI":"10.3390\/ijms231810501","type":"journal-article","created":{"date-parts":[[2022,9,12]],"date-time":"2022-09-12T22:53:46Z","timestamp":1663023226000},"page":"10501","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["New Insights on Heme Uptake in Leishmania spp."],"prefix":"10.3390","volume":"23","author":[{"given":"Mar\u00eda","family":"Cabello-Donayre","sequence":"first","affiliation":[{"name":"Instituto de Parasitolog\u00eda y Biomedicina \u201cL\u00f3pez-Neyra\u201d, Consejo Superior de Investigaciones Cient\u00edficas, PTS Granada, 18016 Granada, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lina M.","family":"Orrego","sequence":"additional","affiliation":[{"name":"Instituto de Parasitolog\u00eda y Biomedicina \u201cL\u00f3pez-Neyra\u201d, Consejo Superior de Investigaciones Cient\u00edficas, PTS Granada, 18016 Granada, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9271-3320","authenticated-orcid":false,"given":"Elisa","family":"Herr\u00e1ez","sequence":"additional","affiliation":[{"name":"Experimental Hepatology and Drug Targeting, Instituto de Investigaci\u00f3n Biom\u00e9dica de Salamanca, University of Salamanca, 37007 Salamanca, Spain"},{"name":"Center for the Study of Liver and Gastrointestinal Diseases, Carlos III National Institute of Health, 28029 Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Raquel","family":"Garc\u00eda-Hern\u00e1ndez","sequence":"additional","affiliation":[{"name":"Instituto de Parasitolog\u00eda y Biomedicina \u201cL\u00f3pez-Neyra\u201d, Consejo Superior de Investigaciones Cient\u00edficas, PTS Granada, 18016 Granada, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0552-5837","authenticated-orcid":false,"given":"Jos\u00e9 M.","family":"P\u00e9rez-Victoria","sequence":"additional","affiliation":[{"name":"Instituto de Parasitolog\u00eda y Biomedicina \u201cL\u00f3pez-Neyra\u201d, Consejo Superior de Investigaciones Cient\u00edficas, PTS Granada, 18016 Granada, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,9,10]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"S10","DOI":"10.1016\/j.jinf.2014.07.016","article-title":"Leishmaniasis","volume":"69","author":"Pace","year":"2014","journal-title":"J. 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