{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,25]],"date-time":"2026-03-25T16:35:05Z","timestamp":1774456505962,"version":"3.50.1"},"reference-count":40,"publisher":"MDPI AG","issue":"18","license":[{"start":{"date-parts":[[2022,9,12]],"date-time":"2022-09-12T00:00:00Z","timestamp":1662940800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Cell Logistics and Research Center, National Research Foundation of Korea","award":["NRF-2016R1A5A1007318"],"award-info":[{"award-number":["NRF-2016R1A5A1007318"]}]},{"name":"Cell Logistics and Research Center, National Research Foundation of Korea","award":["NRF-2020R1A2C2010791"],"award-info":[{"award-number":["NRF-2020R1A2C2010791"]}]},{"name":"Cell Logistics and Research Center, National Research Foundation of Korea","award":["2018R1A6A03025124"],"award-info":[{"award-number":["2018R1A6A03025124"]}]},{"name":"Cell Logistics and Research Center, National Research Foundation of Korea","award":["NRF-2021R1A6A3A13044869"],"award-info":[{"award-number":["NRF-2021R1A6A3A13044869"]}]},{"name":"Cell Logistics and Research Center, National Research Foundation of Korea","award":["20210633"],"award-info":[{"award-number":["20210633"]}]},{"name":"Cell Logistics and Research Center, National Research Foundation of Korea","award":["21-BR-03-05"],"award-info":[{"award-number":["21-BR-03-05"]}]},{"DOI":"10.13039\/501100003566","name":"Ministry of Oceans and Fisheries","doi-asserted-by":"publisher","award":["NRF-2016R1A5A1007318"],"award-info":[{"award-number":["NRF-2016R1A5A1007318"]}],"id":[{"id":"10.13039\/501100003566","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003566","name":"Ministry of Oceans and Fisheries","doi-asserted-by":"publisher","award":["NRF-2020R1A2C2010791"],"award-info":[{"award-number":["NRF-2020R1A2C2010791"]}],"id":[{"id":"10.13039\/501100003566","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003566","name":"Ministry of Oceans and Fisheries","doi-asserted-by":"publisher","award":["2018R1A6A03025124"],"award-info":[{"award-number":["2018R1A6A03025124"]}],"id":[{"id":"10.13039\/501100003566","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003566","name":"Ministry of Oceans and Fisheries","doi-asserted-by":"publisher","award":["NRF-2021R1A6A3A13044869"],"award-info":[{"award-number":["NRF-2021R1A6A3A13044869"]}],"id":[{"id":"10.13039\/501100003566","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003566","name":"Ministry of Oceans and Fisheries","doi-asserted-by":"publisher","award":["20210633"],"award-info":[{"award-number":["20210633"]}],"id":[{"id":"10.13039\/501100003566","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003566","name":"Ministry of Oceans and Fisheries","doi-asserted-by":"publisher","award":["21-BR-03-05"],"award-info":[{"award-number":["21-BR-03-05"]}],"id":[{"id":"10.13039\/501100003566","id-type":"DOI","asserted-by":"publisher"}]},{"name":"KBRI basic research program through the Korea Brain Research Institute","award":["NRF-2016R1A5A1007318"],"award-info":[{"award-number":["NRF-2016R1A5A1007318"]}]},{"name":"KBRI basic research program through the Korea Brain Research Institute","award":["NRF-2020R1A2C2010791"],"award-info":[{"award-number":["NRF-2020R1A2C2010791"]}]},{"name":"KBRI basic research program through the Korea Brain Research Institute","award":["2018R1A6A03025124"],"award-info":[{"award-number":["2018R1A6A03025124"]}]},{"name":"KBRI basic research program through the Korea Brain Research Institute","award":["NRF-2021R1A6A3A13044869"],"award-info":[{"award-number":["NRF-2021R1A6A3A13044869"]}]},{"name":"KBRI basic research program through the Korea Brain Research Institute","award":["20210633"],"award-info":[{"award-number":["20210633"]}]},{"name":"KBRI basic research program through the Korea Brain Research Institute","award":["21-BR-03-05"],"award-info":[{"award-number":["21-BR-03-05"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Alzheimer\u2019s disease (AD), a common form of dementia, is caused in part by the aggregation and accumulation in the brain of amyloid \u03b2 (A\u03b2), a product of the proteolytic cleavage of amyloid precursor protein (APP) in endosomes. Trafficking of APP, such as surface-intracellular recycling, is an early critical step required for A\u03b2 generation. Less is known, however, about the molecular mechanism regulating APP trafficking. This study investigated the mechanism by which SPIN90, along with Rab11, modulates APP trafficking, A\u03b2 motility and accumulation, and synaptic functionality. Brain A\u03b2 deposition was lower in the progeny of 5xFAD-SPIN90KO mice than in 5xFAD-SPIN90WT mice. Analysis of APP distribution and trafficking showed that the surface fraction of APP was locally distinct in axons and dendrites, with these distributions differing significantly in 5xFAD-SPIN90WT and 5xFAD-SPIN90KO mice, and that neural activity-driven APP trafficking to the surface and intracellular recycling were more actively mobilized in 5xFAD-SPIN90KO neurons. In addition, SPIN90 was found to be cotrafficked with APP via axons, with ablation of SPIN90 reducing the intracellular accumulation of APP in axons. Finally, synaptic transmission was restored over time in 5xFAD-SPIN90KO but not in 5xFAD-SPIN90WT neurons, suggesting SPIN90 is implicated in A\u03b2 production through the regulation of APP trafficking.<\/jats:p>","DOI":"10.3390\/ijms231810563","type":"journal-article","created":{"date-parts":[[2022,9,12]],"date-time":"2022-09-12T22:53:46Z","timestamp":1663023226000},"page":"10563","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["SPIN90 Deficiency Ameliorates Amyloid \u03b2 Accumulation by Regulating APP Trafficking in AD Model Mice"],"prefix":"10.3390","volume":"23","author":[{"given":"Youngsoo","family":"Oh","sequence":"first","affiliation":[{"name":"Cell Logistics Research Center, School of Life Science, Gwangju Institute of Science and Technology, Gwangju 61005, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2731-3245","authenticated-orcid":false,"given":"Wongyoung","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Neuroscience, Graduate School, Kyung Hee University, Seoul 02447, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"So Hee","family":"Kim","sequence":"additional","affiliation":[{"name":"Cell Logistics Research Center, School of Life Science, Gwangju Institute of Science and Technology, Gwangju 61005, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sooji","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Medicine, School of Medicine, Kyung Hee University, Seoul 02447, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6827-6730","authenticated-orcid":false,"given":"Byeong C.","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Neurology, Chonnam National University Medical School, Gwangju 61469, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4144-8162","authenticated-orcid":false,"given":"Kun Ho","family":"Lee","sequence":"additional","affiliation":[{"name":"Gwangju Alzheimer\u2019s Disease and Related Dementia Cohort Research Center, Chosun University, Gwangju 61452, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3166-9941","authenticated-orcid":false,"given":"Sung Hyun","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Neuroscience, Graduate School, Kyung Hee University, Seoul 02447, Korea"},{"name":"Department of Physiology, School of Medicine, Kyung Hee University, Seoul 02447, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Woo Keun","family":"Song","sequence":"additional","affiliation":[{"name":"Cell Logistics Research Center, School of Life Science, Gwangju Institute of Science and Technology, Gwangju 61005, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,9,12]]},"reference":[{"key":"ref_1","first-page":"a006189","article-title":"Neuropathological alterations in Alzheimer disease","volume":"1","author":"Frosch","year":"2011","journal-title":"Cold Spring Harb. 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