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Using a mouse model of TSC renal cystogenesis that we have previously described, the current studies delineate the metabolic changes in the kidney and their relation to alterations in renal gene expression. To accomplish this, we compared the metabolome and transcriptome of kidneys from 28-day-old wildtype (Wt) and principal cell-specific Tsc1 KO (Tsc1 KO) mice using targeted 1H nuclear magnetic resonance targeted metabolomic and RNA-seq analyses. The significant changes in the kidney metabolome of Tsc1 KO mice included reductions in the level of several amino acids and significant decreases in creatine, NADH, inosine, UDP-galactose, GTP and myo-inositol levels. These derangements may affect energy production and storage, signal transduction and synthetic pathways. The pertinent derangement in the transcriptome of Tsc1 KO mice was associated with increased collecting duct acid secretion, active cell division and the up-regulation of signaling pathways (e.g., MAPK and AKT\/PI3K) that suppress the TSC2 GTPase-activating function. The combined renal metabolome and transcriptome alterations observed in these studies correlate with the unregulated growth and predominance of genotypically normal A-intercalated cells in the epithelium of renal cysts in Tsc1 KO mice.<\/jats:p>","DOI":"10.3390\/ijms231810601","type":"journal-article","created":{"date-parts":[[2022,9,13]],"date-time":"2022-09-13T21:06:52Z","timestamp":1663103212000},"page":"10601","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Renal Transcriptome and Metabolome in Mice with Principal Cell-Specific Ablation of the Tsc1 Gene: Derangements in Pathways Associated with Cell Metabolism, Growth and Acid Secretion"],"prefix":"10.3390","volume":"23","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4257-5107","authenticated-orcid":false,"given":"Kamyar","family":"Zahedi","sequence":"first","affiliation":[{"name":"Department of Internal Medicine, Division of Nephrology, School of Medicine, University of New Mexico, Albuquerque, NM 87131, USA"},{"name":"Research Services, New Mexico Veterans Health Care Center, Albuquerque, NM 87108, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5710-292X","authenticated-orcid":false,"given":"Sharon","family":"Barone","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, Division of Nephrology, School of Medicine, University of New Mexico, Albuquerque, NM 87131, USA"},{"name":"Research Services, New Mexico Veterans Health Care Center, Albuquerque, NM 87108, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marybeth","family":"Brooks","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, Division of Nephrology, School of Medicine, University of New Mexico, Albuquerque, NM 87131, USA"},{"name":"Research Services, New Mexico Veterans Health Care Center, Albuquerque, NM 87108, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8679-6414","authenticated-orcid":false,"given":"Tracy","family":"Murray Stewart","sequence":"additional","affiliation":[{"name":"The Sidney Kimmel Comprehensive Cancer Center, School of Medicine, Johns Hopkins University, Baltimore, MD 21287, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5653-3306","authenticated-orcid":false,"suffix":"Jr.","given":"Robert","family":"Casero","sequence":"additional","affiliation":[{"name":"The Sidney Kimmel Comprehensive Cancer Center, School of Medicine, Johns Hopkins University, Baltimore, MD 21287, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4909-4469","authenticated-orcid":false,"given":"Manoocher","family":"Soleimani","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, Division of Nephrology, School of Medicine, University of New Mexico, Albuquerque, NM 87131, USA"},{"name":"Research Services, New Mexico Veterans Health Care Center, Albuquerque, NM 87108, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,9,13]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1345","DOI":"10.1056\/NEJMra055323","article-title":"The tuberous sclerosis complex","volume":"355","author":"Crino","year":"2006","journal-title":"N. 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J."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"23433","DOI":"10.1074\/jbc.C500169200","article-title":"Rheb binding to mammalian target of rapamycin (mTOR) is regulated by amino acid sufficiency","volume":"280","author":"Long","year":"2005","journal-title":"J. Biol. Chem."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1457","DOI":"10.1016\/S1097-2765(03)00220-X","article-title":"Insulin activation of Rheb, a mediator of mTOR\/S6K\/4E-BP signaling, is inhibited by TSC1 and 2","volume":"11","author":"Garami","year":"2003","journal-title":"Mol. Cell"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1829","DOI":"10.1101\/gad.1110003","article-title":"Rheb GTPase is a direct target of TSC2 GAP activity and regulates mTOR signaling","volume":"17","author":"Inoki","year":"2003","journal-title":"Genes Dev."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"460","DOI":"10.1177\/0148607115624084","article-title":"Human Protein and Amino Acid Requirements","volume":"40","author":"Hoffer","year":"2016","journal-title":"J. Parenter. Enter. Nutr."},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Amemiya, Y., Nakamura, N., Ikeda, N., Sugiyama, R., Ishii, C., Maki, M., Shibata, H., and Takahara, T. (2021). Amino Acid-Mediated Intracellular Ca(2+) Rise Modulates mTORC1 by Regulating the TSC2-Rheb Axis through Ca(2+)\/Calmodulin. Int. J. Mol. Sci., 22.","DOI":"10.3390\/ijms22136897"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"272","DOI":"10.1016\/j.devcel.2020.08.006","article-title":"The Rag GTPase Regulates the Dynamic Behavior of TSC Downstream of Both Amino Acid and Growth Factor Restriction","volume":"55","author":"Yang","year":"2020","journal-title":"Dev. Cell"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"2342","DOI":"10.1093\/bioinformatics\/btq418","article-title":"MetPA: A web-based metabolomics tool for pathway analysis and visualization","volume":"26","author":"Xia","year":"2010","journal-title":"Bioinformatics"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"2113","DOI":"10.1093\/hmg\/ddy118","article-title":"Metabolomic studies identify changes in transmethylation and polyamine metabolism in a brain-specific mouse model of tuberous sclerosis complex","volume":"27","author":"McKenna","year":"2018","journal-title":"Hum. Mol. Genet."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1137","DOI":"10.1016\/j.chest.2019.05.038","article-title":"Alterations in Polyamine Metabolism in Patients With Lymphangioleiomyomatosis and Tuberous Sclerosis Complex 2-Deficient Cells","volume":"156","author":"Tang","year":"2019","journal-title":"Chest"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"771","DOI":"10.1016\/j.cell.2013.11.049","article-title":"Spatial control of the TSC complex integrates insulin and nutrient regulation of mTORC1 at the lysosome","volume":"156","author":"Menon","year":"2014","journal-title":"Cell"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"11818","DOI":"10.1073\/pnas.1716173114","article-title":"Twenty-five years of mTOR: Uncovering the link from nutrients to growth","volume":"114","author":"Sabatini","year":"2017","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"C836","DOI":"10.1152\/ajpcell.00554.2007","article-title":"A GSK-3\/TSC2\/mTOR pathway regulates glucose uptake and GLUT1 glucose transporter expression","volume":"295","author":"Buller","year":"2008","journal-title":"Am. J. Physiol. Cell Physiol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"487","DOI":"10.1016\/j.molcel.2010.05.007","article-title":"Glucose addiction of TSC null cells is caused by failed mTORC1-dependent balancing of metabolic demand with supply","volume":"38","author":"Choo","year":"2010","journal-title":"Mol. Cell"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"612","DOI":"10.1111\/dmcn.13352","article-title":"Causes of mortality in individuals with tuberous sclerosis complex","volume":"59","author":"Amin","year":"2017","journal-title":"Dev. Med. Child Neurol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"792","DOI":"10.1016\/S0025-6196(12)61196-3","article-title":"Causes of death in patients with tuberous sclerosis","volume":"66","author":"Shepherd","year":"1991","journal-title":"Mayo Clin. Proc."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"e2020190118","DOI":"10.1073\/pnas.2020190118","article-title":"Kidney intercalated cells and the transcription factor FOXi1 drive cystogenesis in tuberous sclerosis complex","volume":"118","author":"Barone","year":"2021","journal-title":"Proc Natl. Acad. Sci. USA"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"e13983","DOI":"10.14814\/phy2.13983","article-title":"Tuberous sclerosis complex exhibits a new renal cystogenic mechanism","volume":"7","author":"Bissler","year":"2019","journal-title":"Physiol. Rep."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"14707","DOI":"10.1038\/s41598-019-51276-9","article-title":"Urinary myo-inositol is associated with the clinical outcome in focal segmental glomerulosclerosis","volume":"9","author":"An","year":"2019","journal-title":"Sci. Rep."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"F755","DOI":"10.1152\/ajprenal.00164.2015","article-title":"Renal depletion of myo-inositol is associated with its increased degradation in animal models of metabolic disease","volume":"309","author":"Chang","year":"2015","journal-title":"Am. J. Physiol. Renal Physiol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.mehy.2016.03.014","article-title":"Does myo-inositol effect on PCOS follicles involve cytoskeleton regulation?","volume":"91","author":"Bizzarri","year":"2016","journal-title":"Med. Hypotheses"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1080\/09513590.2016.1247797","article-title":"Combining treatment with myo-inositol and D-chiro-inositol (40:1) is effective in restoring ovary function and metabolic balance in PCOS patients","volume":"33","author":"Monastra","year":"2017","journal-title":"Gynecol. Endocrinol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"475","DOI":"10.2174\/138920208786241243","article-title":"Genetics and molecular biology of tuberous sclerosis complex","volume":"9","author":"Napolioni","year":"2008","journal-title":"Curr. Genom."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"424","DOI":"10.1016\/j.clnu.2010.02.005","article-title":"Amino acid and protein metabolism in the human kidney and in patients with chronic kidney disease","volume":"29","author":"Garibotto","year":"2010","journal-title":"Clin. Nutr."},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Verzola, D., Picciotto, D., Saio, M., Aimasso, F., Bruzzone, F., Sukkar, S.G., Massarino, F., Esposito, P., Viazzi, F., and Garibotto, G. (2020). Low Protein Diets and Plant-Based Low Protein Diets: Do They Meet Protein Requirements of Patients with Chronic Kidney Disease?. Nutrients, 13.","DOI":"10.3390\/nu13010083"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"32493","DOI":"10.1074\/jbc.C300226200","article-title":"Rheb binds tuberous sclerosis complex 2 (TSC2) and promotes S6 kinase activation in a rapamycin- and farnesylation-dependent manner","volume":"278","author":"Castro","year":"2003","journal-title":"J. Biol. Chem."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1225","DOI":"10.1016\/S0021-9258(17)49882-6","article-title":"Human UDP-galactose 4\u2019-epimerase (GALE) is required for cell-surface glycome structure and function","volume":"295","author":"Broussard","year":"2020","journal-title":"J. Biol. Chem."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"525","DOI":"10.1091\/mbc.E18-04-0247","article-title":"Life is sweet: The cell biology of glycoconjugates","volume":"30","author":"Broussard","year":"2019","journal-title":"Mol. Biol. Cell"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"D608","DOI":"10.1093\/nar\/gkx1089","article-title":"HMDB 4.0: The human metabolome database for 2018","volume":"46","author":"Wishart","year":"2018","journal-title":"Nucleic Acids Res."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1016\/S0378-4347(00)83621-X","article-title":"Solid-phase extraction and determination of dansyl derivatives of unconjugated and acetylated polyamines by reversed-phase liquid chromatography: Improved separation systems for polyamines in cerebrospinal fluid, urine and tissue","volume":"380","author":"Kabra","year":"1986","journal-title":"J. Chromatogr."},{"key":"ref_39","first-page":"3715","article-title":"Correlations between polyamine analogue-induced increases in spermidine\/spermine N1-acetyltransferase activity, polyamine pool depletion, and growth inhibition in human melanoma cell lines","volume":"51","author":"Porter","year":"1991","journal-title":"Cancer Res."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"2496","DOI":"10.1016\/S0021-9258(18)32953-3","article-title":"Changes in mouse kidney ornithine decarboxylase activity are brought about by changes in the amount of enzyme protein as measured by radioimmunoassay","volume":"258","author":"Seely","year":"1983","journal-title":"J. Biol. Chem."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"158","DOI":"10.1016\/S0076-6879(83)94025-9","article-title":"Ornithine decarboxylase (mouse kidney)","volume":"94","author":"Seely","year":"1983","journal-title":"Methods Enzymol."}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/23\/18\/10601\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T00:30:34Z","timestamp":1760142634000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/23\/18\/10601"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,9,13]]},"references-count":41,"journal-issue":{"issue":"18","published-online":{"date-parts":[[2022,9]]}},"alternative-id":["ijms231810601"],"URL":"https:\/\/doi.org\/10.3390\/ijms231810601","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,9,13]]}}}